Smart ungual biopenetrant from the roots of Beta vulgaris

The main objective of the current research was to isolate biopenetrant from Beta vulgaris and formulate bioadhesive layers loaded with terbinafine using this biopenetrant for delivery through nail. Form the preparation of films, Rosa centifolia and Vitis vinifera were used as a biopolymer. Beta vulgaris was used as biopenetrant. The formulations prepared using Beta vulgaris biopenetrant were compared with formulations containing papain as standard penetrant. Four bio-adhesive films of different ratios were prepared by film casting method using each penetrant. The formulated films were evaluated for various parameters like weight, thickness, nail adhesivity, content uniformity, surface pH, folding endurance, and in-vitro drug permeation. The formulation LB4 (containing 1:4 Beta vulgaris biopenetrant) was found to be the best formulation having R2 value 0.990 with zero order as best fit model. The results obtained conclude that biopenetrants can be used as natural penetration enhancer

DEVELOPMENT, CHARACTERIZATION, AND EVALUATION OF SELEGILINE BIONANOSUSPENSIONS USING BUCHANANIA LANZAN AS BIOSTABILIZER

Objective: Development and evaluation of selegiline-loaded bio-nanosuspensions using biopolymer which was isolated from seeds of Buchanania lanzan (Chironji), used as biostabilizer and compared with standard polymer. Methods: The selegiline-loaded bio-nanosuspensions were prepared using novel biopolymer and standard stabilizer (hydroxypropyl methylcellulose) by sonication solvent evaporation method with different ratios (1%, 2%, 3%, 4%, and 5%) and evaluated for particle size, polydispersity index, zeta potential, pH stability studies, percentage entrapment efficacy, in vitro drug release, and stability studies. Results: The prepared selegiline bio-nanosuspensions were subjected to the best formulation based on comparison of above-mentioned evaluation parameters, so Fb2 (2%) formulation was found to be the best formulation showing an R2=0.9842, T50% of 32 h and T80% of 70 h, respectively. According to the release kinetics, the best fit model was found to be Peppas-Korsmeyer with Fickian diffusion (Higuchi matrix) as the mechanism of drug release, and Fs5 (5%) formulation was found to be the best formulation showing an R2=0.9564, T50% of 25 h and T80% of 60 h, respectively. According to the release kinetics, the best fit model was found to be Peppas-Korsmeyer with Fickian diffusion (Higuchi matrix) as the mechanism of drug release. The biopolymer provided excellent stability for the formulation and resulting particle size for the best formulation was found to be 360 nm. The best formulation was found to be polydispersity index of 0.43 with zeta potential of −5.12 mV. Conclusion: The prepared bio-nanosuspensions using biopolymer were found to be safe and compatible with the novel drug delivery for the treatment of depression in comparison of standard polymer

SMART INNOVATIVE APPROACH FOR DESIGNING FLUVOXAMINE LOADED BIO-NANOSUSPENSION FOR THE MANAGEMENT OF DEPRESSION

Objective: Design and evaluation of fluvoxamine loaded bio-nanosuspensions using biopolymer which was isolated from the wood of Santalum album used as the stabilizer.Methods: The main aim of the present investigation was to obtain an ocular drug delivery system with improved stability using biopolymer. The fluvoxamine loaded Bio-nanosuspension was prepared using novel biopolymer isolated from Santalum album by sonication solvent evaporation method with different ratios (1%, 2%, 3%, 4% and 5%) and evaluated for particle size, polydispersity index, zeta potential, pH stability studies, %entrapment efficacy, in vitro drug release, stability studies.Results: The prepared bio-nanosuspension was subjected to the best formulation based on the comparison of above-mentioned evaluation parameters, so Fb3 (3%) formulation was found to be the best formulation showing an R2 value of 0.9744, T50% of 31.3 h and T80% of 50.1 h respectively. According to the release kinetics, the best fit model was found to be Peppas Korsmeyer with Fickian Diffusion (Higuchi Matrix) as the mechanism of drug release. Santalum album provided excellent stability for the formulation, and resulting particle size for the best formulation was found to be 196 nm. The bio-nanosuspension had Polydispersity Index (PDI) of 0.19 with zeta potential of-20mV.Conclusion: The prepared bio-nanosuspension was found to be safe and compatible with the ophthalmic delivery for treatment of depression

SAFETY AND EFFICACY STUDY OF HERBAL POLYPHYTO FORMULATIONS: FOR ITS LEARNING AND MEMORY ENHANCING PROPERTIES

Objective: The present study was designed to evaluate the effectiveness of the poly photo formulation for its learning and memory activity.Methods: The Indian origin test drug, FM7 phyto formulation compose of Convolulus pluricaulis, Habiscus rosasinnsis, Withania somnifera, Terminalia arjuna and Emblica officinalis, having the potential effect to improving memory studied at a dose of 50 mg and 100 mg/kg p. o. by using three different animal model like Elevated plus maze (EPM), Morris water maze (MWM) and Pole Climbing apparatus (PCA) for the effect of nootropic action; against standard drug Bacopa monnieri evaluated on the basis of transfer latency reduction on a rat, before and after drug administration.Results: On treatment with polyherbal formulation FM7 showed a significant effect on enhancing learning and memory properties. It was observed that significant (p<0.001) reduction in transfer latency in Elevated Plus Maze, Morris Water Maze test and escape latency in Pole Climbing Apparatus test as compared with the control; as well as standard Bacopa monnieri. Conclusion: The polyphyto formulation FM7 composed of (Convolulus pluricaulis 20%, Habiscus rosasinnsis 20%, Withania somnifera 20%, Terminalia arjuna 20% and Emblica officinalis 20%) found to be safe and effective in enhancing learning and memory properties.Keywords: Learning and memory, Convolulus pluricaulis, Phyllanthus Emblica, Withania somnifera, Habiscus rosasinnsis, Terminalia arjun

EXTRACTION AND CHARACTERIZATION OF NOVEL BIOADHESANT BIOMATERIAL OBTAINED FROM THE PHASEOLUS VULGARIS

Objective: The objective of the present study was to isolate bioadhesive biomaterial from the pulp of Phaseolus vulgaris and further characterization includes preliminary phytochemical screening, physicochemical parameter micromeritic properties, spectral analysis (like NMR, X-Ray diffraction, DSC and IR spectroscopy) and acute toxicity study. The work also emphasizes to evaluate the mucoadhesive and in built properties of isolated biopolymer. Methods: Phaseolus vulgaris biopolymer was obtained using non solvent addition method. Pharmacopoeial procedures were used to study the micromeritic properties, solubility, organoleptic properties, pH, viscosity, swelling index and surface characteristics of isolated biopolymer. The mucoadhesive property was determined by Shear stress method, falling sphere method and rotating basket method and data obtained were compared with standard. Results: The result showed that isolated biomaterial exhibited good flow behavior and pH was found 5.6 showed that this can be used in oral formulations without any irritation. SEM analysis suggests that the biomaterial has irregular particle size and XRD pattern of the biopolymer indicated a completely amorphous structure. The formation of hydrogen bond by natural bioadhesive agent with nasal mucosa was confirmed by FTIR spectra showing carboxyl and hydroxyl groups further mucoadhesive assessment suggest that the isolated biomaterial exhibited polymeric characteristics with promising inbuilt mucoadhesive property. Conclusion: The research study revealed that the biomaterial isolated from pulp of Phaseolus vulgaris exhibited a promishing potent natural mucoadhesive property and may be used to develop mucoadhesive transmucosal drug delivery systems

Cell line toxicity study and pharmacological screening of effective nootropic herbal formulation in rat

The polyphyto drugs FM27; compose of Mentha piperita (10%), Ribes nigrum (20%), Cinnamomum zeylanicum (20%), Aloe vera (10%), Zingiber officinalis (5%), Glycine max (5%) and Avena sativa (30%) on memory acquisition and retention was studied using EPM, MWM and PCA test in rat using two different routes, one is per oral (FM27O) and another one is transcranial (FM27T). The test drug studied for in vitro safety profile screening by MTT assay in a different cell line. As per the result, cell line toxicity confirmed that formulation FM27 was non toxic in SH-SY-5Y human neuroblastoma cell line, Vero monkey normal kidney epithelial cells, and Chang liver cell line. Test drug FM27O studied at a dose of (PO 50 and 100 mg/kg), showed graded dose response and it was observed significantly (p <0.01) effect of memory improvement comparing standard group containing Bacopa monnieri (PO 50 and 100 mg/kg), whereas transcranially applied FM27T (10 mg/kg) showed a significant (p <0.001) effect of memory improvement comparing control group and observed similar effect like standard drug. Herbal formulations FM27 was found to be potent for treating dementia to improve memory. The cell line toxicity study report confirmed it was safe in higher dose also

Cell line toxicity study and pharmacological screening of effective nootropic herbal formulation in rat

23-30The polyphyto drugs FM27; compose of Mentha piperita (10%), Ribes nigrum (20%), Cinnamomum zeylanicum (20%), Aloe vera (10%), Zingiber officinalis (5%), Glycine max (5%) and Avena sativa (30%) on memory acquisition and retention was studied using EPM, MWM and PCA test in rat using two different routes, one is per oral (FM27O) and another one is transcranial (FM27T). The test drug studied for in vitro safety profile screening by MTT assay in a different cell line. As per the result, cell line toxicity confirmed that formulation FM27 was non toxic in SH-SY-5Y human neuroblastoma cell line, Vero monkey normal kidney epithelial cells, and Chang liver cell line. Test drug FM27O studied at a dose of (PO 50 and 100 mg/kg), showed graded dose response and it was observed significantly (p Bacopa monnieri (PO 50 and 100 mg/kg), whereas transcranially applied FM27T (10 mg/kg) showed a significant (p <0.001) effect of memory improvement comparing control group and observed similar effect like standard drug. Herbal formulations FM27 was found to be potent for treating dementia to improve memory. The cell line toxicity study report confirmed it was safe in higher dose also