Comparative analysis of bones, mites, soil chemistry, nematodes and soil micro-Eukaryotes from a suspected homicide to estimate the post-mortem interval

Criminal investigations of suspected murder cases require estimating the post-mortem interval (PMI, or time after death) which is challenging for longer periods. Here we present the case of human remains found in a Swiss forest. We have used a multidisciplinary approach involving the analysis of bones, soil chemical characteristics, mites and nematodes (by microscopy) and micro-Eukaryotes (by Illumina high throughput sequencing). We analysed soil samples collected beneath the remains of the head, upper and lower body and “control” samples taken a few meters away. The PMI estimated on hair 14C-data via bomb peak radiocarbon dating gave a time range of 1 to 2 years before the finding of the remains on site. Cluster analyses for chemical constituents, nematodes, mites and micro- Eukaryotes revealed two clusters 1) head and upper body and 2) lower body and controls. From mite evidence, we conclude that the body was likely to have been brought to the site after death. However, chemical analyses, nematode community analyses and the analyses of micro-Eukaryotes indicate that decomposition took place at least partly on site. This study illustrates the usefulness of combining several lines of evidence for the study of homicide cases to better calibrate PMI inference tools

Th/U dating of frozen peat, Bol'shoy Lyakhovsky Island (northern Siberia)

The chronology of Quaternary paleoenvironment and climate in northeastern Siberia is poorly understood due to a lack of reliable numerical age determinations. The best climatic archives are ice-rich permafrost sequences, which are widely distributed in northeastern Siberia. For this study, 230Th/U-ages were determined by thermal ionization mass spectrometry (TIMS) from frozen peat in a permafrost deposit at the southern cliff of the Bol'shoy Lyakhovsky Island (New Siberian Archipelago), west of the Zimov'e River. These yielded a Pre-Eemian "isochron"-corrected 230Th/U- age of 200,900 ± 3400 yr. This result is reliable because permafrost deposits behave as closed systems with respect to uranium and thorium. Our findings suggest that 230Th/U dating of frozen peat in permafrost deposits is a useful tool for the reconstruction of the Middle Quaternary environment of northern Siberia and of the whole Arctic. © 2002 University of Washington