46 research outputs found
The carpase-like sites of proteasomes: substrate specificity, new inhibitors and substrates, and allosteric interactions with the trypsin-like sites
Bio-Organische synthes
A switchable controlled-NOT gate in a spin-chain NMR quantum computer
A method of switching a controlled-NOT gate in a solid-stae NMR quantum
computer is presented. Qubits of I=1/2 nuclear spins are placed periodically
along a quantum spin chain (1-D antiferromagnet) having a singlet ground state
with a finite spin gap to the lowest excited state caused by some quantum
effect. Irradiation of a microwave tuned to the spin gap energy excites a
packet of triplet magnons at a specific part of the chain where control and
target qubits are involved. The packet switches on the Suhl-Nakamura
interaction between the qubits, which serves as a controlled NOT gate. The
qubit initialization is achieved by a qubit initializer consisting of
semiconducting sheets attached to the spin chain, where spin polarizations
created by the optical pumping method in the semiconductors are transferred to
the spin chain. The scheme allows us to separate the initialization process
from the computation, so that one can optimize the computation part without
being restricted by the initialization scheme, which provides us with a wide
selection of materials for a quantum computer.Comment: 8 pages, 5 figure
Kv1.3 Channel Up-Regulation in Peripheral Blood T Lymphocytes of Patients With Multiple Sclerosis
Voltage-gated Kv1.3 potassium channels are key regulators of T lymphocyte activation, proliferation and cytokine production, by providing the necessary membrane hyper-polarization for calcium influx following immune stimulation. It is noteworthy that an accumulating body of in vivo and in vitro evidence links these channels to multiple sclerosis pathophysiology. Here we studied the electrophysiological properties and the transcriptional and translational expression of T lymphocyte Kv1.3 channels in multiple sclerosis, by combining patch clamp recordings, reverse transcription polymerase chain reaction and flow cytometry on freshly isolated peripheral blood T lymphocytes from two patient cohorts with multiple sclerosis, as well as from healthy and disease controls. Our data demonstrate that T lymphocytes in MS, manifest a significant up-regulation of Kv1.3 mRNA, Kv1.3 membrane protein and Kv1.3 current density and therefore of functional membrane channel protein, compared to control groups (p < 0.001). Interestingly, patient sub-grouping shows that Kv1.3 channel density is significantly higher in secondary progressive, compared to relapsing-remitting multiple sclerosis (p < 0.001). Taking into account the tight connection between Kv1.3 channel activity and calcium-dependent processes, our data predict and could partly explain the reported alterations of T lymphocyte function in multiple sclerosis, while they highlight Kv1.3 channels as potential therapeutic targets and peripheral biomarkers for the disease. © Copyright © 2021 Markakis, Charitakis, Beeton, Galani, Repousi, Aggeloglou, Sfikakis, Pennington, Chandy and Poulopoulou