Serum biochemical status and morphological changes in mice ovary associated with copper oxide nanoparticles after thiamine therapy

Introduction: Nanoparticles (NPs) can induce inflammatory responses and oxidative stress and are also cytotoxic to the genital organs of animals after exposure. The aim of this study was to assess the effects of copper oxide (CuO) and CuO NPs alone and in combination with thiamine on the ovaries of mice and on antioxidant enzymes.Methods: Sixty adult mice were randomly divided into five groups. Group A served as the control. Group B received CuO NPs and group C received CuO at 0.2 mL/kg intraperitoneally (IP). Mice in groups D and E respectively received CuO and CuO NPs along with thiamine (30 mg/L) therapy. The responses of the ovaries to the treatments were appraised by histopathology studies. The values for catalase (CAT), superoxide dismutase (SOD), and lipid peroxidation were determined after 20 days of treatment.Results: The degree of degeneration and apoptosis of the different zones within the ovaries were recorded in groups B and C. The decrease in CAT value and increase in SOD activity were significant for groups B and C at 20 days compared to the control group. The thiobarbituric acid reactive substances (TBARS) level in groups B, C and E were significantly higher at 20th day when compared with control group. The groups treated with thiamine showed histopathological and enzymatic results that were similar to those of the control group.Conclusion: These findings suggest the combination of CuO NPs and CuO with thiamine improves serum enzyme activity and has positive effects on the ovary

Investigation and determination of marine biotoxins in the shellfish of Persian Gulf and Oman Sea

Marine algal toxins have drawn worldwide attention because of their involvement in human intoxication and the socio-economic impacts. Marine biotoxins have been produced by harmful bloom algae, known as dinoflagellate. In the present study, two groups of toxins, i.e. PSP, ASP analyzed in the muscle of shellfish caught from the north parts of the Persian Gulf (Bandar Abbas, Bandar Lengeh, Boushehr) and Oman Sea (Chabahar). Sample preparation and extraction were done according to AOAC methods and by ELISA. PSP amounts in the shellfish samples ranged from ND-3.962 and ND-1.477 mg/g muscle. The results showed all samples were safe

Adjustment formulae to improve the correlation of white-to-white measurement with direct measurement of the ciliary sulcus diameter by ultrasound biomicroscopy

Purpose: This study evaluates the correlation between horizontal white-to-white (WTW) distance using Caliper and Orbscan IIz with the ciliary sulcus diameter measured by high frequency ultrasound biomicroscopy (UBM) and presents an adjustment formula to improve the correlation. Methods: We measured horizontal sulcus-to-sulcus (STS) dimension of 273 right eyes of 273 high myopic patients with 35 MHz UBM and horizontal WTW using Orbscan IIz and Caliper. Mean WTW diameter, differences, and the correlation of measurement methods were evaluated. Results: The mean spherical equivalent was �8.79 ± 4.87 diopters. Mean horizontal STS dimension with UBM was 12.13 ± 0.45 mm (range, 10.81�13.42 mm). Mean WTW diameter in the Caliper method was 11.70 ± 0.40 mm (range, 10.6�12.8 mm) and 11.70 ± 0.40 mm (range, 10.5�13.1 mm) in the Orbscan method. Mean difference of UBM STS and WTW with Caliper was 0.48 ± 0.28 mm (range, �0.19 to 1.37 mm). Mean difference of UBM STS diameter and Orbscan WTW was 0.38 ± 0.31 mm (range, �0.64 to 1.29 mm). The Pearson correlations of WTW diameter measured by Caliper and Orbscan with UBM's STS diameter were 0.778 and 0.773, respectively. This difference diminished after adjustment. The 95 limit of agreement was almost the same in Caliper and Orbscan (�0.07 to 1.03 compared with �0.23 to 0.99). Conclusion: There is a significant difference in measurements between STS diameter using UBM and WTW diameter utilizing Caliper and Orbscan. This difference diminished after our recommended adjustment. © 2017 Iranian Society of Ophthalmolog

Oncolytic Newcastle disease virus delivered by Mesenchymal stem cells-engineered system enhances the therapeutic effects altering tumor microenvironment

Background: Human papillomavirus (HPV)-associated malignancy remain a main cause of cancer in men and women. Cancer immunotherapy has represented great potential as a new promising cancer therapeutic approach. Here, we report Mesenchymal stem cells (MSCs) as a carrier for the delivery of oncolytic Newcastle disease virus (NDV) for the treatment of HPV-associated tumor. Methods: For this purpose, MSCs obtained from the bone marrow of C57BL mice, then cultured and characterized subsequently by the flow cytometry analysis for the presence of cell surface markers. In this study, we sought out to determine the impacts of MSCs loaded with oncolytic NDV on splenic T cell and cytokine immune responses, caspase-3 and -9 expression, and myeloid and myeloid-derived suppressor cells (MDSCs) by histological and immunohistochemical studies in the tumor microenvironment (TME). Results: Our findings proved that MSCs possess both migratory capacity and tumor tropism toward transplanted tumor tissue after peritumoral administration. Tumor therapy experiments indicated that oncolytic NDV delivered by MSCs-engineered system significantly reduces tumor growth, which is associated with the enhancement of E7-specific lymphocyte proliferation, CD8+ T cell cytolysis responses, and splenic IFN-γ, IL-4 and IL-12 responses compared with control groups. Moreover, the treatment upregulated the concentration of apoptotic proteins (caspase 9) and increased infiltration of tumor microenvironment with CD11b + myeloid and Gr1 + MDSCs cells. Conclusions: Our data suggest MSCs carrying oncolytic NDV as a potentially effective strategy for cancer immunotherapy through inducing splenic Th1 immune responses and apoptosis in the tumor microenvironment. © 2020 The Author(s)

Environmental impacts of shrimp farms on coastal waters in Tiab area, Hormozgan province, south of Iran

Shrimp farming industry has rapidly expanded in the south of Iran and in particular in Honnozgan Province along the coastal line during the past decade. A survey was conducted for evaluation of environmental impacts of effluents from the shrimp farms during culture season (July-December 2003) of Tiab area in Hormozgan Province. The physicochemical parameters such as air and water temperature, salinity, pH, dissolved oxygen, BOD5, nitrite, nitrate, total ammonia, inorganic phosphorus and total phosphorus were recorded monthly from 9 stations. The stations consisted of two inlets (stations 13 3), two outlets of effluents (stations 2, 4) and the remaining 5 stations were located along the coastal lines. The parameters were measured at 21-40°C, 22on39°C, 37-54ppt, 7.85-8.38, 4-8.1mg/L, 0.2-8.5mg/L, 0.11-0 .186mg/L, 0.001-0.029mg/L, 0- 0.016mg/L, Ome0.043mg/L and 0.014-1.4mg/L) respectively. The results showed that the range of most of the measured parameters such as water temperature, salinity, BOD5, ammonia, nitrate, phosphate and TP in the outflow waters (2, 4) were higher than inflow (1, 3) and coastal waters (5, 6, 7, 8 ,9). Statistical analysis of variance (ANOVA) demonstrated a significant difference between outlet effluent and other stations (P<0.05). We also concluded that the nutrients in the culture effluents are somewhat used by fauna of the Tiab creek, hence decreasing the pollutant load of the effluent

CRISPR-Cas systems in Proteus mirabilis

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) is a bacterial defense mechanism against bacteriophages composed of two different parts: the CRISPR array and the Cas genes. The spacer acquisition is done by the adaptation module consisting of the hallmark Cas1 Cas2 proteins, which inserts new spacers into the CRISPR array. Here we aimed to describe the CRISPR-Cas system in Proteus mirabilis (P. mirabilis) isolates. CRISPR loci was observed in 30 genomic contents of 109 P. mirabilis isolates that each locus was consisted of two CRISPR arrays and each array had a different preserved leader sequences. Only the type I-E CRISPR-Cas system was common in these isolates. The source of the spacers was identified, including phages and prophages. CRISPR spacer origin analysis also identified a conserved PAM sequence of 5�-AAG-3� nucleotide stretch. Through collecting spacers, CRISPR arrays of P. mirabilis isolates were expanded mostly by integration of bacteriophageal source of spacers. This study shows novel findings in the area of the P-mirabilis CRISPR-Cas system. In this regard, among analyzed genome of P. mirabilis isolates, Class I CRISR-Cas systems were dominant, and all belonged to type I-E. In the flanks of the CRISPR, some other elements with regulatory role were also found. A motif of 11 nt size was found to be preserved among the analyzed genome. We believe that it might has a CRISPR-Cas system transcription facilitator by targeting the Rho element. © 2021 Elsevier B.V

Non-replicating Newcastle Disease Virus as an adjuvant for DNA vaccine enhances antitumor efficacy through the induction of TRAIL and granzyme B expression

The potential of non-replicating Newcastle Disease Virus (NDV) as an adjuvant for DNA vaccination remains to be elucidated. To assess the therapeutic effects of DNA vaccine (HPV-16 E7 gene) adjuvanted with NDV, female C57/BL6 mice were inoculated with murine TC-1 cells of human papillomavirus (HPV)-related carcinoma, expressing human papillomavirus 16 (HPV-16) E6/E7 antigens, and immunized with DNA vaccine alone or pretreated with NDV. One week after third immunization, Cytotoxic T lymphocytes (CTLs), splenocyte proliferation, cytokine balance (IFN-γ IL-4 and IL-12 secretions) and intratumoral expression of cytotoxicity related proteins in tumor lysates were investigated. The results showed that treatment with non-replicating NDV prior to DNA vaccine induced tumor-specific cytolytic and splenocyte proliferation responses. The levels of cytokines IL-12, IL-4 and IFN�γ after treating with combined E7-DNA -non-replicating NDV (NDV-DNA Vaccine) were significantly higher than those of control groups. The intratumoral granzyme B and Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)-mediated apoptosis was also significantly increased. Tumor therapeutic experiments showed that the NDV pretreatment could reduce the tumor progression of established E7-expressing TC-tumors. Taken together these data suggest that the significant antitumor responses evidenced during treatment with non-replicating NDV prior to DNA vaccine are due, in part, to strong E7-induced cellular immunity and enhanced expression of cytotoxicity related proteins in the tumor microenvironment. These observations indicated the potential of non-replicating NDV as an adjuvant for enhancing therapeutic DNA vaccines -induced immunity and antitumor responses. © 2018 Elsevier B.V

Correction to: Oncolytic Newcastle disease virus delivered by Mesenchymal stem cells-engineered system enhances the therapeutic effects altering tumor microenvironment (Virology Journal, (2020), 17, 1, (64), 10.1186/s12985-020-01326-w)

An amendment to this paper has been published and can be accessed via the original article. © 2020, The Author(s)