390 research outputs found
Fusion of secretory vesicles isolated from rat liver
Secretory vesicles isolated from rat liver were found to fuse after exposure to Ca2+. Vescle fusion is characterized by the occurrence of twinned vesicles with a continuous cleavage plane between two vesicles in freeze-fracture electron microscopy. The number of fused vesicles increases with increasing Ca2+-concentrations and is half maximal around 10â6 m. Other divalent cations (Ba2+, Sr2+, and Mg2+) were ineffective. Mg2+ inhibits Ca2+-induced fusion. Therefore, the fusion of secretory vesiclesin vitro is Ca2+ specific and exhibits properties similar to the exocytotic process of various secretory cells.
Various substances affecting secretionin vivo (microtubular inhibitors, local anethetics, ionophores) were tested for their effect on membrane fusion in our system.
The fusion of isolated secretory vesicles from liver was found to differ from that of pure phospholipid membranes in its temperature dependence, in its much lower requirement for Ca2+, and in its Ca2+-specificity. Chemical and enzymatic modifications of the vesicle membrane indicate that glycoproteins may account for these differences
Inhomogeneous chiral symmetry breaking in noncommutative four fermion interactions
The generalization of the Gross-Neveu model for noncommutative 3+1 space-time
has been analyzed. We find indications that the chiral symmetry breaking occurs
for an inhomogeneous background as in the LOFF phase in condensed matter.Comment: 17 pages, 2 figures, published version, minor correction
The outer halos of elliptical galaxies
Recent progress is summarized on the determination of the density
distributions of stars and dark matter, stellar kinematics, and stellar
population properties, in the extended, low surface brightness halo regions of
elliptical galaxies. With integral field absorption spectroscopy and with
planetary nebulae as tracers, velocity dispersion and rotation profiles have
been followed to ~4 and ~5-8 effective radii, respectively, and in M87 to the
outer edge at ~150 kpc. The results are generally consistent with the known
dichotomy of elliptical galaxy types, but some galaxies show more complex
rotation profiles in their halos and there is a higher incidence of
misalignments, indicating triaxiality. Dynamical models have shown a range of
slopes for the total mass profiles, and that the inner dark matter densities in
ellipticals are higher than in spiral galaxies, indicating earlier assembly
redshifts. Analysis of the hot X-ray emitting gas in X-ray bright ellipticals
and comparison with dynamical mass determinations indicates that non-thermal
components to the pressure may be important in the inner ~10 kpc, and that the
properties of these systems are closely related to their group environments.
First results on the outer halo stellar population properties do not yet give a
clear picture. In the halo of one bright galaxy, lower [alpha/Fe] abundances
indicate longer star formation histories pointing towards late accretion of the
halo. This is consistent with independent evidence for on-going accretion, and
suggests a connection to the observed size evolution of elliptical galaxies
with redshift.Comment: 8 pages. Invited review to appear in the proceedings of "Galaxies and
their Masks" eds. Block, D.L., Freeman, K.C. & Puerari, I., 2010, Springer
(New York
Inflation from Warped Space
A long period of inflation can be triggered when the inflaton is held up on
the top of a steep potential by the infrared end of a warped space. We first
study the field theory description of such a model. We then embed it in the
flux stabilized string compactification. Some special effects in the throat
reheating process by relativistic branes are discussed. We put all these
ingredients into a multi-throat brane inflationary scenario. The resulting
cosmic string tension and a multi-throat slow-roll model are also discussed.Comment: 39 pages; v4, added reference, to appear in JHE
Spontaneous Creation of Inflationary Universes and the Cosmic Landscape
We study some gravitational instanton solutions that offer a natural
realization of the spontaneous creation of inflationary universes in the brane
world context in string theory. Decoherence due to couplings of higher
(perturbative) modes of the metric as well as matter fields modifies the
Hartle-Hawking wavefunction for de Sitter space. Generalizing this new
wavefunction to be used in string theory, we propose a principle in string
theory that hopefully will lead us to the particular vacuum we live in, thus
avoiding the anthropic principle. As an illustration of this idea, we give a
phenomenological analysis of the probability of quantum tunneling to various
stringy vacua. We find that the preferred tunneling is to an inflationary
universe (like our early universe), not to a universe with a very small
cosmological constant (i.e., like today's universe) and not to a 10-dimensional
uncompactified de Sitter universe. Such preferred solutions are interesting as
they offer a cosmological mechanism for the stabilization of extra dimensions
during the inflationary epoch.Comment: 52 pages, 7 figures, 1 table. Added discussion on supercritical
string vacua, added reference
Cosmic F- and D-strings
Macroscopic fundamental and Dirichlet strings have several potential
instabilities: breakage, tachyon decays, and confinement by axion domain walls.
We investigate the conditions under which metastable strings can exist, and we
find that such strings are present in many models. There are various
possibilities, the most notable being a network of (p,q) strings. Cosmic
strings give a potentially large window into string physics.Comment: 27 pages, 5 figures; v. 5: JHEP style, added comments in section 2.
Pretreatment dietary intake is associated with tumor suppressor DNA methylation in head and neck squamous cell carcinomas
Diet is associated with cancer prognosis, including head and neck cancer (HNC), and has been hypothesized to influence epigenetic state by determining the availability of functional groups involved in the modification of DNA and histone proteins. The goal of this study was to describe the association between pretreatment diet and HNC tumor DNA methylation. Information on usual pretreatment food and nutrient intake was estimated via food frequency questionnaire (FFQ) on 49 HNC cases. Tumor DNA methylation patterns were assessed using the Illumina Goldengate Methylation Cancer Panel. First, a methylation score, the sum of individual hypermethylated tumor suppressor associated CpG sites, was calculated and associated with dietary intake of micronutrients involved in one-carbon metabolism and antioxidant activity, and food groups abundant in these nutrients. Second, gene specific analyses using linear modeling with empirical Bayesian variance estimation were conducted to identify if methylation at individual CpG sites was associated with diet. All models were controlled for age, sex, smoking, alcohol and HPV status. Individuals reporting in the highest quartile of folate, vitamin B12 and vitamin A intake, compared with those in the lowest quartile, showed significantly less tumor suppressor gene methylation, as did patients reporting the highest cruciferous vegetable intake. Gene specific analyses identified differential associations between DNA methylation and vitamin B12 and vitamin A intake when stratifying by HPV status. These preliminary results suggest that intake of folate, vitamin A and vitamin B12 may be associated with the tumor DNA methylation profile in HNC and enhance tumor suppression
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Pretreatment dietary intake is associated with tumor suppressor DNA methylation in head and neck squamous cell carcinomas
Diet is associated with cancer prognosis, including head and neck cancer (HNC), and has been hypothesized to influence epigenetic state by determining the availability of functional groups involved in the modification of DNA and histone proteins. The goal of this study was to describe the association between pretreatment diet and HNC tumor DNA methylation. Information on usual pretreatment food and nutrient intake was estimated via food frequency questionnaire (FFQ) on 49 HNC cases. Tumor DNA methylation patterns were assessed using the Illumina Goldengate Methylation Cancer Panel. First, a methylation score, the sum of individual hypermethylated tumor suppressor associated CpG sites, was calculated and associated with dietary intake of micronutrients involved in one-carbon metabolism and antioxidant activity, and food groups abundant in these nutrients. Second, gene specific analyses using linear modeling with empirical Bayesian variance estimation were conducted to identify if methylation at individual CpG sites was associated with diet. All models were controlled for age, sex, smoking, alcohol and HPV status. Individuals reporting in the highest quartile of folate, vitamin B12 and vitamin A intake, compared with those in the lowest quartile, showed significantly less tumor suppressor gene methylation, as did patients reporting the highest cruciferous vegetable intake. Gene specific analyses identified differential associations between DNA methylation and vitamin B12 and vitamin A intake when stratifying by HPV status. These preliminary results suggest that intake of folate, vitamin A and vitamin B12 may be associated with the tumor DNA methylation profile in HNC and enhance tumor suppression
Brane Inflation, Solitons and Cosmological Solutions: I
In this paper we study various cosmological solutions for a D3/D7 system
directly from M-theory with fluxes and M2-branes. In M-theory, these solutions
exist only if we incorporate higher derivative corrections from the curvatures
as well as G-fluxes. We take these corrections into account and study a number
of toy cosmologies, including one with a novel background for the D3/D7 system
whose supergravity solution can be completely determined. This new background
preserves all the good properties of the original model and opens up avenues to
investigate cosmological effects from wrapped branes and brane-antibrane
annihilation, to name a few. We also discuss in some detail semilocal defects
with higher global symmetries, for example exceptional ones, that could occur
in a slightly different regime of our D3/D7 model. We show that the D3/D7
system does have the required ingredients to realise these configurations as
non-topological solitons of the theory. These constructions also allow us to
give a physical meaning to the existence of certain underlying homogeneous
quaternionic Kahler manifolds.Comment: Harvmac, 115 pages, 9 .eps figures; v2: typos corrected, references
added and the last section expanded; v3: Few minor typos corrected and
references added. Final version to appear in JHE
Oralism: a sign of the times? The contest for deaf communication in education provision in late nineteenth-century Scotland
Disability history is a diverse field. In focussing upon children within deaf education in late nineteenth-century Scotland, this essay reflects some of that diversity. In 1880, the International Congress on the Education of the Deaf in Milan stipulated that speech should have âpreferenceâ over signs in the education of deaf children. The mode of achieving this, however, effectively banned sign language. Endeavours to teach deaf children to articulate were not new, but this decision placed pressures on deaf institutions to favour the oral system of deaf communication over other methods. In Scotland efforts were made to adopt oralism, and yet educators were faced with the reality that this was not good educational practice for most pupils. This article will consider responses of Scottish educators of deaf children from the 1870s until the beginning of the twentieth century
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