Toll-like Receptor 2 and 4 (TLR2 and TLR4) Agonists Differentially Regulate Secretory Interleukin-1 Receptor Antagonist Gene Expression in Macrophages

Treatment of macrophages with lipopolysaccharide (LPS) from Gram-negative bacteria or peptidoglycan (PGN) from Gram-positive bacteria activates multiple intracellular signaling pathways and a large, diverse group of nuclear transcription factors. The signaling receptors for PGN and LPS are now known to be the Toll-like receptors 2 and 4 (TLR2 and -4, respectively). While a large body of literature indicates that the members of the TLR family activate nearly identical cytoplasmic signaling programs, several recent reports have suggested that the functional outcomes of signaling via TLR2 or TLR4 are not equivalent. In the current studies, we compared the responses of the secretory IL-1 receptor antagonist (sIL-1Ra) gene to both LPS and PGN. Both LPS and PGN induced IL-1Ra gene expression; however, the combination of both stimuli synergistically increased sIL-1Ra mRNA expression and promoter activity, suggesting that the signals induced by PGN and LPS are not equivalent. While both LPS and PGN utilized the PU.1-binding sites in the proximal sIL-1Ra promoter region to generate a full response, additional distinct promoter elements were utilized by LPS or PGN. Activation of p38 stress-activated protein kinase was required for LPS- or PGN-induced IL-1Ra gene expression, but the p38-responsive promoter elements localized to distinct regions of the sIL-1Ra gene. Additionally, while the LPS-induced, p38-dependent response was dependent upon PU.1 binding, the PGN-induced, p38 response was not. Collectively, these data indicated that while some of the intracellular signaling events by TLR2 and TLR4 agonists are similar, there are clearly distinct differences in the responses elicited by these two bacterial products

Eta invariants for flat manifolds

Using H. Donnelly result from the article "Eta Invariants for G-Spaces" we calculate the eta invariants of the signature operator for almost all 7-dimensional flat manifolds with cyclic holonomy group. In all cases this eta invariants are an integer numbers. The article was motivated by D. D. Long and A. Reid article "On the geometric boundaries of hyperbolic 4-manifolds, Geom. Topology 4, 2000, 171-178Comment: 18 pages, a new version with referees comment

Representations and KK-theory of Discrete Groups

Let $\Gamma$ be a discrete group of finite virtual cohomological dimension with certain finiteness conditions of the type satisfied by arithmetic groups. We define a representation ring for $\Gamma$, determined on its elements of finite order, which is of finite type. Then we determine the contribution of this ring to the topological $K$-theory $K^*(B\Gamma)$, obtaining an exact formula for the difference in terms of the cohomology of the centralizers of elements of finite order in $\Gamma$.Comment: 4 page

Accelerating the Universe with Gravitational Waves

Inflation generically produces primordial gravitational waves with a red spectral tilt. In this paper we calculate the backreaction produced by these gravitational waves on the expansion of the universe. We find that in radiation domination the backreaction acts as a relativistic fluid, while in matter domination a small dark energy emerges with an equation of state w=-8/9.Comment: 18 pages, 4 figures. Replaced with version published by JCAP - some discussion and references added concerning second-order gravitational waves, typeset in JHEP styl

Gauges and Cosmological Backreaction

We present a formalism for spatial averaging in cosmology applicable to general spacetimes and coordinates, and allowing the easy incorporation of a wide variety of matter sources. We apply this formalism to a Friedmann-LeMaitre-Robertson-Walker universe perturbed to second-order and present the corrections to the background in an unfixed gauge. We then present the corrections that arise in uniform curvature and conformal Newtonian gauges.Comment: 13 pages. Updated: reference added, typos corrected, exposition clarified. Version 3: Replaced with version published by JCA

Inundation of Stormwater Infrastructure Is Common and Increases Risk of Flooding in Coastal Urban Areas Along the US Atlantic Coast

Stormwater infrastructure can manage precipitation-driven flooding when there are no obstructions to draining. Coastal areas increasingly experience recurrent flooding due to elevated water levels from storms or tides, but the inundation of coastal stormwater infrastructure by elevated water levels has not been broadly assessed. We conservatively estimated stormwater infrastructure inundation in municipalities along the Atlantic United States coast by using areas of high-tide flooding (HTF) on roads as a proxy. We also modeled stormwater infrastructure inundation in four North Carolina municipalities and measured infrastructure inundation in one of the modeled municipalities. Combining methodologies at different scales provides context and allows the scope of stormwater infrastructure inundation to be broadly estimated. We found 137 census-designated urban areas along the Atlantic coast with road area impacted by HTF, with a median percent of total road area subject to HTF of 0.16% (IQR: 0.02%–0.53%). Based on 2010 census block data, the median number of people per urban area that live in census blocks with HTF on roads was 1,622 (IQR: 366–5,779). In total, we estimate that over 2 million people live in census blocks where HTF occurs on roadways along the US Atlantic coast. Modeling results and water level measurements indicated that extensive inundation of underground stormwater infrastructure likely occurs at water levels within the mean tidal range. These results suggest that stormwater infrastructure inundation along the US Atlantic coast is likely widespread, affects a large number of people, occurs frequently, and increases the occurrence of urban flooding

Anisotropic Superparamagnetism of Monodispersive Cobalt-Platinum Nanocrystals

Based on the high-temperature organometallic route (Sun et al. Science 287, 1989 (2000)), we have synthesized powders containing CoPt_3 single crystals with mean diameters of 3.3(2) nm and 6.0(2) nm and small log-normal widths sigma=0.15(1). In the entire temperature range from 5 K to 400 K, the zero-field cooled susceptibility chi(T) displays significant deviations from ideal superparamagnetism. Approaching the Curie temperature of 450(10) K, the deviations arise from the (mean-field) type reduction of the ferromagnetic moments, while below the blocking temperature T_b, chi(T) is suppressed by the presence of energy barriers, the distributions of which scale with the particle volumes obtained from transmission electron microscopy (TEM). This indication for volume anisotropy is supported by scaling analyses of the shape of the magnetic absorption chi''(T,omega) which reveal distribution functions for the barriers being also consistent with the volume distributions observed by TEM. Above 200 K, the magnetization isotherms M(H,T) display Langevin behavior providing 2.5(1) mu_B per CoPt_3 in agreement with reports on bulk and thin film CoPt_3. The non-Langevin shape of the magnetization curves at lower temperatures is for the first time interpreted as anisotropic superparamagnetism by taking into account an anisotropy energy of the nanoparticles E_A(T). Using the magnitude and temperature variation of E_A(T), the mean energy barriers and 'unphysical' small switching times of the particles obtained from the analyses of chi''(T,omega) are explained. Below T_b hysteresis loops appear and are quantitatively described by a blocking model, which also ignores particle interactions, but takes the size distributions from TEM and the conventional field dependence of E_A into account.Comment: 12 pages with 10 figures and 1 table. Version accepted for publication in Phys. Rev. B . Two-column layou