14 research outputs found
Calcium oxalate crystal adherence to hyaluronan-, osteopontin-, and CD44-expressing injured/regenerating tubular epithelial cells in rat kidneys
Retention of crystals in the kidney is an essential early step in renal
stone formation. Studies with renal tubular cells in culture indicate that
hyaluronan (HA) and osteopontin (OPN) and their mutual cell surface
receptor CD44 play an important role in calcium oxalate (CaOx) crystal
binding during wound healing. This concept was investigated in vivo by
treating rats for 1, 4, and 8 d with ethylene glycol (0.5 and 0.75%) in
their drinking water to induce renal tubular cell damage and CaOx
crystalluria. Tubular injury was morphologically scored on periodic
acid-Schiff-stained renal tissue sections and tissue repair assessed by
immunohistochemical staining for proliferating cell nuclear antigen. CaOx
crystals were visualized in periodic acid-Schiff-stained sections by
polarized light microscopy, and renal calcium deposits were quantified
with von Kossa staining. HA was visualized with HA-binding protein and OPN
and CD44 immunohistochemically with specific antibodies and quantified
with an image analyzer system. Already after 1 d of treatment, both
concentrations of ethylene glycol induced hyperoxaluria and CaOx
crystalluria. At this point, there was neither tubular injury nor crystal
retention in the kidney, and expression of HA, OPN, and CD44 was
comparable to untreated controls. After 4 and 8 d of ethylene glycol,
however, intratubular crystals were found adhered to injured/regenerating
(proliferating cell nuclear antigen positive) tubular epithelial cells,
expressing HA, OPN, and CD44 at their luminal membrane. In conclusion, the
expression of HA, OPN, and CD44 by injured/regenerating tubular cells
seems to play a role in retention of crystals in the rat kidney
Quantitative Relationships between Body Weight, Kidney Weight and Nephron Size in Mongrel Dogs
Variations in measured alkaline phosphatase activity: influence of isoenzymes and buffer systems109
Propylene glycol-induced side effects during intravenous nitroglycerin therapy111
Propylene glycol, an alcohol frequently used as a solvent in medical preparations, is considered non-toxic. We found that this solvent, used in a commercially available IV nitroglycerin solution, may cause hyperosmolality, hemolysis and lactic acidosis. The influence of kidney function as the main determinant in causing accumulation of this solvent and consequently hyperosmolality is emphasized. A review of the literature dealing with propylene glycol is given. The possible mechanisms of neurological disturbances occurring during IV nitroglycerin therapy are discussed</p