Non-destructive Assessment Of Chlorophyll-deficient Mutants Of Maize (zea Mays L.) By Photoacoustic Spectroscopy

Photoacoustic spectroscopy is an efficient tool for the non-destructive assessment of chlorophyll and carotenoid deficient mutants of Zea mays L. Intact leaf samples taken from the mutants Albina, Luteus I and Luteus II were compared with those from the corresponding isogenic normals. The results are compared with those obtained applying some commonly used destructive techniques. © 1985.3814751Prioli, (1981) M.Sc. Thesis, , 2nd edn., State University of Campinas, San Paulo, Brazil, (in Portuguese)Holden, Chlorophylls (1976) Chemistry and Biochemistry of Plant Pigments, 2. , 2nd edn., T.W. Goodwin, Academic Press, New YorkDavies, Carotenoids (1976) Chemistry and Biochemistry of Plant Pigments, 2. , 2nd edn., T.W. Goodwin, Academic Press, New YorkBell, (1980) Am. J. Sci., 20, p. 305Viengerov, (1938) Dokl. Akad. Nauk SSSR, 19, p. 687Rosencwaig, (1973) Science, 181, p. 657Pao, (1980) Optoacoustic spectroscopy and detection, , Academic Press, New YorkRosencwaig, (1980) Photoacoustics and Photoacoustic Spectroscopy, , J. Wiley and Sons, New YorkKinney, Staley, (1982) Annu. Rev. Mater. Sci., 12, p. 295McClelland, Photoacoustic spectroscopy (1983) Analytical Chemistry, 55, p. 89Cahen, Bults, Garty, Malkin, (1980) J. Biochem. Biophys. Methods, 3, p. 293Lima, Mattos, Miranda, Penna, von Bulow, Ghizoni, (1982) J. Photoacoustics, 1, p. 61Lasser-Ross, Malkin, Cahen, (1980) Biochim. Biophys. Acta, 593, p. 330Bults, Horwitz, Malkin, Cahen, (1982) Biochim. Biophys. Acta, 679, p. 452Poulet, Cahen, Malkin, (1983) Biochim. Biophys. Acta, 724, p. 433Kanstad, Cahen, Malkin, (1983) Biochim. Biophys. Acta, 722, p. 18

Fonsecaea pedrosoi cerebral phaeohyphomycosis ("chromoblastomycosis"): first human culture-proven case reported in Brazil Feohifomicose cerebral ("cromoblastomicose") por Fonsecaea pedrosoi: primeiro caso demonstrado por cultura do fungo no Brasil

Cerebral phaeohyphomycosis ("chromoblastomycosis") is a rare intracranial lesion. We report the first human culture-proven case of brain abscesses due to Fonsecaea pedrosoi in Brazil. The patient, a 28 year-old immunocompetent white male, had ocular manifestations and a hypertensive intracranial syndrome. Magnetic resonance imaging (MRI) of the brain revealed a main tumoral mass involving the right temporo-occipital area and another smaller apparently healed lesion at the left occipital lobe. A cerebral biopsy was performed and the pathological report was cerebral chromoblastomycosis. The main lesion was enucleated surgically and culture of the necrotic and suppurative mass grew a fungus identified as Fonsecaea pedrosoi. The patient had received a knife wound sixteen years prior to his hospitalization and, more recently, manifested a pulmonary granulomatous lesion in the right lung with a single non-pigmented form of a fungus present. It was speculated that the fungus might have gained entrance to the host through the skin lesion, although a primary respiratory lesion was not excluded. The patient was discharged from the hospital still with ocular manifestations and on antimycotic therapy and was followed for eight months without disease recurrence. Few months after he had complications of the previous neuro-surgery and died. A complete autopsy was performed and no residual fungal disease was found.<br>A Feohifomicose cerebral ("cromoblastomicose") é uma lesão rara. Apresentamos o primeiro caso desta entidade com cultura do abscesso cerebral, devido a Fonsecaea pedrosoi. O paciente, um homem de 28 anos de idade, imunocompetente, apresentou manifestações oculares e síndrome de hipertensão intracraniana. Imagens de ressonância magnética (MRI) cerebral mostraram massa tumoral envolvendo a área temporo-occipital direita e outra lesão menor, possivelmente cicatricial, no lobo occipital esquerdo. Biopsia cerebral mostrou cromoblastomicose cerebral. A lesão principal foi enucleada cirurgicamente e Fonsecaea pedrosoi foi cultivado da massa necrótica e supurada. O paciente tinha tido um ferimento por faca 16 anos antes de ser visto no nosso hospital e, mais recentemente, uma lesão pulmonar granulomatosa no pulmão direito onde foi identificada uma forma isolada, não pigmentada, de fungo. A possibilidade de que o fungo tenha penetrado no organismo do paciente através da lesão de pele foi considerada, mas não se pode excluir uma eventual lesão primária pulmonar. O paciente teve alta hospitalar e foi seguido durante oito meses sem recorrência da doença. Meses depois, entretanto, desenvolveu complicações relacionadas à neurocirurgia, vindo a óbito. Uma autopsia completa foi feita, não demonstrando doença fúngica remanescente