Cloning and expression of highly pathogenic avian influenza virus full-length nonstructural gene in Pichia pastoris.

Avian influenza (AI) is a highly contagious and rapidly evolving pathogen of major concern to the poultry industry and human health. Rapid and accurate detection of avian influenza virus is a necessary tool for control of outbreaks and surveillance. The AI virus A/Chicken/Malaysia/5858/2004 (H5N1) was used as a template to produce DNA clones of the full-length NS1 genes via reverse transcriptase synthesis of cDNA by PCR amplification of the NS1 region. Products were cloned into pCR2.0 TOPO TA plasmid and subsequently subcloned into pPICZA vector to construct a recombinant plasmid. Recombinant plasmid designated as pPICZA-NS1 gene was confirmed by PCR colony screening, restriction enzyme digestion, and nucleotide sequence analysis. The recombinant plasmid was transformed into Pichia pastoris GS115 strain by electroporation, and expressed protein was identified by SDS-PAGE and western blotting. A recombinant protein of approximately ~28kDa was produced. The expressed protein was able to bind a rabbit polyclonal antibody of nonstructural protein (NS1) avian influenza virus H5N1. The result of the western blotting and solid-phase ELISA assay using H5N1 antibody indicated that the recombinant protein produced retained its antigenicity. This further indicates that Pichia pastoris could be an efficient expression system for a avian influenza virus nonstructural (NS1)

Review of Manufacturing Process of Natural Fiber Reinforced Polymer Composites

Lately, natural fibers have been gaining consideration in development of composites due to its biodegradability, availability and low-cost compared to synthetic fibers. Natural fibers are renewable sources, biodegradable, environmentally friendly and effortless in machinery. In this paper, the manufacturing of production for natural fiber reinforced polymer composite (NFRC) will be reviewed. These including injection molding, resin transfer molding (RTM), pultrusion, sheet molding compound (SMC) and compression molding processing technique. Injection molding and compression molding are mostly used in the industry. This is due to the machines give a good finishing surface of the products and low cos

Mechanical, thermal and morphological properties of epoxy resin toughened with epoxidized soybean oil

Biobased toughened thermosetting polymer blend was prepared by incorporating epoxidized soybean oil (ESO) into a petroleum-based epoxy (DGEBA) in different composition ratios. The mechanical properties (tensile and flexural tests) of the ESO/DGEBA thermoset blends were determined. Thermal properties of the blends were characterized using thermogravimetric analysis. The result showed that, the tensile and flexural properties decreased with increasing of ESO content. However, a slight increase in the strength properties was observed at 10% of ESO content. A significant enhancement in impact strength proves the role of ESO acting as a plasticizer in the blends as well as improve the toughness properties of ESO/DGEBA thermoset blend. As the ESO content increase, the thermal stability of ESO/DGEBA thermoset blend has decreased might be due to reduced cross-linking density of the epoxy network. Further investigations on morphological properties were also done to correlate the mechanical properties of ESO/DGEBA thermoset blend

Effect of polymer composition on the physiochemical properties of microencapsulated fish oil using spray drying

Fish oil are considered as a good source of omega-3 polyunsaturated fatty acids (PUFAs) which have been shown to reduce the risk of coronary heart disease, hypertension, thrombosis, inflammations, rheumatoid arthritis, symptoms of allergies (atopic eczema), some types of cancer and the rate of ageing, and promote the development and functions of central nervous system. In the current study, HPMC 15 cP and HPMC 5 cP were chosen with different concentration as a carrier material