Glucosylation of chimeric proteins in the cell wall of Saccharomyces cerevisiae

AbstractExtension of a reporter protein with the carboxyterminal thirty amino acids of the cell wall mannoprotein α-agglutinin of Saccharomyces cerevisiae resulted in incorporation of the chimeric protein in the cell wall. By Western analysis it was shown that the incorporated protein contained β-1,6-glucan similar to endogenous cell wall proteins, whereas excreted reporter protein was not glucosylated. This suggests that β-1,6-glucan is involved in anchoring mannoproteins in the cell wall

Poverty is Bad: Ways forward in livelihood research

In this paper, we take the view that the essence of poverty is the fact that one or several basic conditions for generating a living are not being met. The conditions considered most vital for making a living are to a certain extent context specific and subject to (cultural and social) interpretation and evaluation