9 research outputs found
Π£ΡΠΏΠ΅ΡΠ½ΡΠΉ ΠΎΠΏΡΡ Π»Π΅ΡΠ΅Π½ΠΈΡ ΠΏΠ΅ΡΠ²ΠΈΡΠ½ΠΎΠΉ ΠΊΠΎΠΆΠ½ΠΎΠΉ Π°Π½Π°ΠΏΠ»Π°ΡΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΡΡΠΏΠ½ΠΎΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ Π»ΠΈΠΌΡΠΎΠΌΡ, ΠΏΡΠΎΡΠ΅ΠΊΠ°ΡΡΠ΅ΠΉ Ρ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Π½ΡΠΌ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΠ΅ΠΌ ΠΊΠΎΠΆΠ½ΠΎΠ³ΠΎ ΠΏΠΎΠΊΡΠΎΠ²Π° ΠΈ Π»Π΅Π³ΠΎΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ
The aim of the study is to present a successful case in treating primary cutaneous anaplastic large cellΒ lymphoma (PCALCL) occurring with common lesions of the skin and lung tissue.Materials and methods. For the verification of the diagnosis in a patient with three types of skin elementsΒ (spot, thin plaque with and without ulceration), differential diagnosis was performed between ulcerativeΒ pyoderma gangrenosum, PCALCL, large-cell transformation of mycosis fungoides, and secondary skinΒ lesions under the nodal ALK-negtaive ALCL. A complex of studies, including histological, immunohisto -Β chemical, cytogenetic studies of skin tumor biopsy, allowed the verification of the PCALCL diagnosis. ForΒ the treatment of the patient, intensive induction chemotherapy was used followed by high-dose consolidation and autologous transplantation of hematopoietic stem cells.Results. The selected treatment tactics allowed a long-term complete remission of the disease to beΒ achieved in a patient from the poor prognosis group.Conclusion. An algorithm for the differential diagnosis and tactics of treating is presented for a patient withΒ primary anaplastic large cell lymphoma with a widespread skin lesion and extradermal foci.Π¦Π΅Π»Ρ: Π΄Π΅ΠΌΠΎΠ½ΡΡΡΠ°ΡΠΈΡ ΡΡΠΏΠ΅ΡΠ½ΠΎΠ³ΠΎ ΠΎΠΏΡΡΠ° Π»Π΅ΡΠ΅Π½ΠΈΡ ΠΏΠ΅ΡΠ²ΠΈΡΠ½ΠΎΠΉ ΠΊΠΎΠΆΠ½ΠΎΠΉ Π°Π½Π°ΠΏΠ»Π°ΡΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΡΡΠΏΠ½ΠΎΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ Π»ΠΈΠΌΡΠΎΠΌΡ (ΠΏΠΊΠΠΠΠ), ΠΏΡΠΎΡΠ΅ΠΊΠ°ΡΡΠ΅ΠΉ Ρ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Π½ΡΠΌ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΠ΅ΠΌ ΠΊΠΎΠΆΠ½ΠΎΠ³ΠΎ ΠΏΠΎΠΊΡΠΎΠ²Π° ΠΈ Π»Π΅Π³ΠΎΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠ»Ρ Π²Π΅ΡΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ Π΄ΠΈΠ°Π³Π½ΠΎΠ·Π° Ρ Π±ΠΎΠ»ΡΠ½ΠΎΠ³ΠΎ Ρ ΡΡΠ΅ΠΌΡ Π²ΠΈΠ΄Π°ΠΌΠΈ ΠΊΠΎΠΆΠ½ΡΡ
ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ²Β (ΠΏΡΡΠ½ΠΎ, ΡΠΎΠ½ΠΊΠ°Ρ Π±Π»ΡΡΠΊΠ° Ρ ΠΈΠ·ΡΡΠ·Π²Π»Π΅Π½ΠΈΠ΅ΠΌ ΠΈ Π±Π΅Π· Π½Π΅Π³ΠΎ) ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»Π°ΡΡ Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½Π°Ρ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠ°Β ΠΌΠ΅ΠΆΠ΄Ρ ΡΠ·Π²Π΅Π½Π½ΠΎ-Π³Π°Π½Π³ΡΠ΅Π½ΠΎΠ·Π½ΠΎΠΉ ΠΏΠΈΠΎΠ΄Π΅ΡΠΌΠΈΠ΅ΠΉ, ΠΏΠΊΠΠΠΠ, ΠΊΡΡΠΏΠ½ΠΎΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ ΡΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΈΠ΅ΠΉ Π³ΡΠΈΠ±ΠΎΠ²ΠΈΠ΄Π½ΠΎΠ³ΠΎ ΠΌΠΈΠΊΠΎΠ·Π° ΠΈ Π²ΡΠΎΡΠΈΡΠ½ΡΠΌ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΠ΅ΠΌ ΠΊΠΎΠΆΠΈ ΠΏΡΠΈ Π½ΠΎΠ΄Π°Π»ΡΠ½ΠΎΠΉ ΠΠΠΠ, ALK-. ΠΡΠΎΠ²Π΅Π΄Π΅Π½Π½ΡΠΉ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΒ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ, Π²ΠΊΠ»ΡΡΠ°ΡΡΠΈΠΉ Π³ΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ΅, ΠΈΠΌΠΌΡΠ½ΠΎΠ³ΠΈΡΡΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠ΅, ΡΠΈΡΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π±ΠΈΠΎΠΏΡΠ°ΡΠ° ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΊΠΎΠΆΠΈ, ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΠ» Π²Π΅ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°ΡΡ Π΄ΠΈΠ°Π³Π½ΠΎΠ· ΠΏΠΊΠΠΠΠ. ΠΠ»Ρ Π»Π΅ΡΠ΅Π½ΠΈΡ Π±ΠΎΠ»ΡΠ½ΠΎΠ³ΠΎΒ Π±ΡΠ»Π° ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½Π° ΠΈΠ½ΡΠ΅Π½ΡΠΈΠ²Π½Π°Ρ ΠΈΠ½Π΄ΡΠΊΡΠΈΠΎΠ½Π½Π°Ρ Ρ
ΠΈΠΌΠΈΠΎΡΠ΅ΡΠ°ΠΏΠΈΡ Ρ ΠΏΠΎΡΠ»Π΅Π΄ΡΡΡΠ΅ΠΉ Π²ΡΡΠΎΠΊΠΎΠ΄ΠΎΠ·Π½ΠΎΠΉ ΠΊΠΎΠ½ΡΠΎΠ»ΠΈΠ΄Π°ΡΠΈΠ΅ΠΉ ΠΈ Π°ΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ½ΠΎΠΉ ΡΡΠ°Π½ΡΠΏΠ»Π°Π½ΡΠ°ΡΠΈΠ΅ΠΉ Π³Π΅ΠΌΠΎΠΏΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΡΠ²ΠΎΠ»ΠΎΠ²ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΡΠ±ΡΠ°Π½Π½Π°Ρ ΡΠ°ΠΊΡΠΈΠΊΠ° Π»Π΅ΡΠ΅Π½ΠΈΡ ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΠ»Π° Π΄ΠΎΡΡΠΈΠ³Π½ΡΡΡ ΠΏΡΠΎΠ΄ΠΎΠ»ΠΆΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ ΠΏΠΎΠ»Π½ΠΎΠΉ ΡΠ΅ΠΌΠΈΡΡΠΈΠΈΒ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ Ρ Π±ΠΎΠ»ΡΠ½ΠΎΠ³ΠΎ ΠΈΠ· Π³ΡΡΠΏΠΏΡ Π½Π΅Π±Π»Π°Π³ΠΎΠΏΡΠΈΡΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΠ³Π½ΠΎΠ·Π°.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Ρ Π°Π»Π³ΠΎΡΠΈΡΠΌ Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ ΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΠ°ΠΊΡΠΈΠΊΠΈΒ Π»Π΅ΡΠ΅Π½ΠΈΡ Π±ΠΎΠ»ΡΠ½ΠΎΠ³ΠΎ ΠΏΠ΅ΡΠ²ΠΈΡΠ½ΠΎΠΉ ΠΊΠΎΠΆΠ½ΠΎΠΉ Π°Π½Π°ΠΏΠ»Π°ΡΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΡΡΠΏΠ½ΠΎΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ Π»ΠΈΠΌΡΠΎΠΌΠΎΠΉ Ρ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Π½ΡΠΌ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΠ΅ΠΌ ΠΊΠΎΠΆΠ½ΠΎΠ³ΠΎ ΠΏΠΎΠΊΡΠΎΠ²Π° ΠΈ ΡΠΊΡΡΡΠ°Π΄Π΅ΡΠΌΠ°Π»ΡΠ½ΡΠΌΠΈ ΠΎΡΠ°Π³Π°ΠΌΠΈ
Phylogenomics and the rise of the angiosperms
Angiosperms are the cornerstone of most terrestrial ecosystems and human livelihoods1,2. A robust understanding of angiosperm evolution is required to explain their rise to ecological dominance. So far, the angiosperm tree of life has been determined primarily by means of analyses of the plastid genome3,4. Many studies have drawn on this foundational work, such as classification and first insights into angiosperm diversification since their Mesozoic origins5,6,7. However, the limited and biased sampling of both taxa and genomes undermines confidence in the tree and its implications. Here, we build the tree of life for almost 8,000 (about 60%) angiosperm genera using a standardized set of 353 nuclear genes8. This 15-fold increase in genus-level sampling relative to comparable nuclear studies9 provides a critical test of earlier results and brings notable change to key groups, especially in rosids, while substantiating many previously predicted relationships. Scaling this tree to time using 200 fossils, we discovered that early angiosperm evolution was characterized by high gene tree conflict and explosive diversification, giving rise to more than 80% of extant angiosperm orders. Steady diversification ensued through the remaining Mesozoic Era until rates resurged in the Cenozoic Era, concurrent with decreasing global temperatures and tightly linked with gene tree conflict. Taken together, our extensive sampling combined with advanced phylogenomic methods shows the deep history and full complexity in the evolution of a megadiverse clade
Do protein crystals nucleate within dense liquid clusters?
Protein-dense liquid clusters are regions of high protein concentration that have been observed in solutions of several proteins. The typical cluster size varies from several tens to several hundreds of nanometres and their volume fraction remains below 10-3 of the solution. According to the two-step mechanism of nucleation, the protein-rich clusters serve as locations for and precursors to the nucleation of protein crystals. While the two-step mechanism explained several unusual features of protein crystal nucleation kinetics, a direct observation of its validity for protein crystals has been lacking. Here, two independent observations of crystal nucleation with the proteins lysozyme and glucose isomerase are discussed. Firstly, the evolutions of the protein-rich clusters and nucleating crystals were characterized simultaneously by dynamic light scattering (DLS) and confocal depolarized dynamic light scattering (cDDLS), respectively. It is demonstrated that protein crystals appear following a significant delay after cluster formation. The cDDLS correlation functions follow a Gaussian decay, indicative of nondiffusive motion. A possible explanation is that the crystals are contained inside large clusters and are driven by the elasticity of the cluster surface. Secondly, depolarized oblique illumination dark-field microscopy reveals the evolution from liquid clusters without crystals to newly nucleated crystals contained in the clusters to grown crystals freely diffusing in the solution. Collectively, the observations indicate that the protein-rich clusters in lysozyme and glucose isomerase solutions are locations for crystal nucleation
Phylogenomics and the rise of the angiosperms
Angiosperms are the cornerstone of most terrestrial ecosystems and human livelihoods1,2. A robust understanding of angiosperm evolution is required to explain their rise to ecological dominance. So far, the angiosperm tree of life has been determined primarily by means of analyses of the plastid genome3,4. Many studies have drawn on this foundational work, such as classification and first insights into angiosperm diversification since their Mesozoic origins5,6,7. However, the limited and biased sampling of both taxa and genomes undermines confidence in the tree and its implications. Here, we build the tree of life for almost 8,000 (about 60%) angiosperm genera using a standardized set of 353 nuclear genes8. This 15-fold increase in genus-level sampling relative to comparable nuclear studies9 provides a critical test of earlier results and brings notable change to key groups, especially in rosids, while substantiating many previously predicted relationships. Scaling this tree to time using 200 fossils, we discovered that early angiosperm evolution was characterized by high gene tree conflict and explosive diversification, giving rise to more than 80% of extant angiosperm orders. Steady diversification ensued through the remaining Mesozoic Era until rates resurged in the Cenozoic Era, concurrent with decreasing global temperatures and tightly linked with gene tree conflict. Taken together, our extensive sampling combined with advanced phylogenomic methods shows the deep history and full complexity in the evolution of a megadiverse clade