Cytotoxicity and antitumoral activity of dichloromethane extract and its fractions from Pothomorphe umbellata

The cytotoxicity of the dichloromethane crude extract (DCE), obtained from the aerial parts of Pothomorphe umbellata (L.) Miq (Piperaceae), was evaluated against nine human cancer cell lines (MCF-7, NCI-ADR/RES, OVCAR-3, PC-3, HT-29, NCI-H460, 786-O, UACC-62, K-562). The DCE presented antiproliferative activity with good potency against all cell lines at low concentrations (between 4.0 and 9.5 µg/mL) and with selectivity (1.55 µg/mL) for the leukemia cell line (K-652). DCE (100, 200, 300 and 400 mg/kg, ip) was also evaluated in the Ehrlich ascites tumor model. Both the survival number and the life span of the animals that died increased by at least 45 and 50%, respectively (8 animals per group), demonstrating P. umbellata extract potential anticancer activity. The results of the in vivo antitumor activity prompted the fractionation of the crude extract. The crude extract was submitted to dry column chromatography with dichloromethane-methanol (99:1). The column effluent fractions were extracted with methanol, dried under vacuum yielding fractions FR1 (less polar), FR2 (medium polarity), and FR3 (polar), which were analyzed for their growth inhibition or cytotoxic properties by a 48-h sulforhodamine B cell viability assay by measuring the total protein content. FR1 demonstrated high potency and cytotoxicity, a result compatible with the high toxicity of oxalic acid; FR2, containing 4-nerolidylcathecol, presented the lowest cytotoxic activity compared to the other two fractions but with selectivity for prostate cancer cell line; FR3, containing a mixture of steroids described in the literature as possessing various biological activities, also presented potent anticancer in vitro activity. These results suggest that P. umbellata DCE in vivo antitumor activity may be a consequence of the activity of different active principles.41141

Correlação entre caracteres agronômicos, fisiológicos e fitoquímicos em variedades de Artemisia annua L

The aim of this study was to estimate the correlations among agronomic, physiological and phytochemical traits of two varieties of Artemisia annua and to identify correlations that can be used in selection and breeding processes for this species. The design was completely randomized and the treatments were for Artemisia annua varieties 2/39x5x3M and 2/39x1V, which were subjected to agronomic, physiological and phytochemical evaluations. The relationship among the studied traits was estimated with Pearson's correlation coefficient, and the significance level of correlations was established by the t-test using Genes software. A significant positive correlation was found between the traits canopy volume and essential oil yield for 2/39x5x3M variety, showing that the selection of plants with a larger canopy volume would facilitate indirect selection of the trait essential oil yield. This correlation was not found for the 2/39x1V variety. In both varieties, there was a significant negative correlation between canopy volume and artemisinin content, which suggested that the selection of plants with a large canopy volume and a large number of branches should be avoided if the goal is to increase artemisinin content.O objetivo deste trabalho foi estimar as correlações existentes entre caracteres agronômicos, fisiológicos e fitoquímicos em duas variedades de Artemisia annua e identificar correlações que possam ser utilizadas em processos de seleção e melhoramento da espécie. O delineamento foi inteiramente casualizado e os tratamentos foram às variedades 2/39x5x3M e 2/39x1V de Artemisia annua, submetidas a avaliações agronômicas, fisiológicas e fitoquímicas. A relação existente entre os caracteres estudados foi estimada através do coeficiente de correlação de Pearson e o nível de significância das correlações pelo teste t, realizadas no programa Genes. Encontrou-se correlação positiva e significativa entre os caracteres volume de dossel e rendimento de óleo essencial na variedade 2/39x5x3M, evidenciando que a seleção de plantas com maior volume de dossel possibilitaria a seleção indireta para o cárater rendimento de óleo essencial. Esta correlação não foi encontrada na variedade 2/39x1V. Em ambas as variedades, observou-se correlação negativa e significativa entre volume de dossel e teor de artemisinina indicando que deve-se evitar a seleção de plantas com grande volume de dossel e número de ramificações, se o interesse do melhoramento for maior teor de artemisinina.135141Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

Synthesis, in vitro antiproliferative and anti-mycobacterium tuberculosis activities of novel β-carboline derivatives

A series of β-carboline derivatives with amino or guanidinium were synthesized and evaluated in vitro against anti-Mycobacterium tuberculosis and for antiproliferative activities against nine human cancer cell lines. The compounds 1-(4-hydroxyphenyl)-3-carboxamide(ethylamine) β-carboline (24.9 μg mL-1) and 1-(4-methoxyphenyl)-3-carboxamide(ethylamine) β-carboline (26.9 μg mL-1) were the most active against M. Tuberculosis (MTB). Compounds 1-(4-hydroxyphenyl)-3-carboxamide(ethylamine) β-carboline and 1-(4-methoxyphenyl)-3-carboxamide(propylamine) β-carboline, which had the same substituted groups, inhibited the growth of all human tumor cell lines with growth inhibitory activity (GI50) values from 1.37 to 9.20 mmol L-1. Also in this series, compounds 1-(4-hydroxyphenyl)-3-carboxamide(propylamine) β-carboline and 1-(3-nitrophenyl)-3-carboxamide(propylamine) β-carboline demonstrated significant activity against NCI/ADR cells. Among compounds with a terminal guanidine group, compounds 1-(4-hydroxyphenyl)-3-carboxamide(ethyl)guanidine β-carboline (27.8 μg mL-1) and 1-(3-nitrophenyl)-3-carboxamide(ethyl) guanidine β-carboline (37.4 μg mL-1) demonstrated the greatest activity against MTB. Additionally, compounds 1-(4-methoxyphenyl)-3-carboxamide(ethyl)guanidine β-carboline (GI50 = 0.45 mmol L-1) effectively inhibited growth and was highly selective against NCI/ADR. The in silico study revealed that 1-(4-hydroxyphenyl)-3-carboxamide(ethylamine) β-carboline, 1-(4-methoxyphenyl)-3-carboxamide(ethylamine) β-carboline, 1-(4-hydroxyphenyl)-3-carboxamide(propylamine) β-carboline, 1-(4-methoxyphenyl)-3-carboxamide(propylamine) β-carboline and 1-(3-nitrophenyl)-3-carboxamide(propylamine) β-carboline compounds follow the rules established by Lipinski, suggesting that this compound has no problems with oral bioavailability.27813981405CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESSem informaçãoSem informaçã

Docosahexaenoic Acid Ethyl Esther (dhaee) Microcapsule Production By Spray-drying: Optimization By Experimental Design [obtenção De Microcápsulas Do éster Etílico Do ácido Docosa-hexaenoico (dhaee) Por Atomização: Otimização Através De Planejamento Experimental]

Docosahexaenoic acid is an essential polyunsaturated fatty acid with important metabolic activities. Its conjugated double bonds make it susceptible to decomposition. Its stability may be improved through fatty acid entrapment with a spray-drying technique; however, the many parameters involved in this technique must be considered to avoid affecting the final product quality. Therefore, this study aimed to evaluate the entrapment conditions and yields of fish oil enriched with docosahexaenoic acid ethyl ester. Microcapsules were obtained from Acacia gum using a spray-drying technique. The experimental samples were analyzed by chromatography and delineated by Statistica software, which found the following optimum entrapment conditions: an inlet temperature of 188 °C; 30% core material; an N 2 flow rate of 55 mm; and a pump flow rate of 12.5 mL/minute. These conditions provided a 66% yield of docosahexaenoic acid ethyl ester in the oil, corresponding to 19.8% of entrapped docosahexaenoic acid ethyl ester (w/w). This result was considered significant since 30% corresponded to wall material.313589596Ahn, J.H., Optimization of microencapsulation of seed oil by response surface methodology (2008) Food Chemistry, 107, pp. 98-105. , http://dx.doi.org/10.1016/j.foodchem.2007.07.067Bertolini, A.C., Siani, A.C., Grosso, C.R.F., Stability of monoterpenes of encapsulated in gum arabic by spray drying (2001) Journal of Agricultural and Food Chemistry, 49 (2), pp. 780-785. , http://dx.doi.org/10.1021/jf000436y, PMid:11262028Botelho, M.L.R., (1999) Propriedades Físico- Químicas Do Exsudato De Anacardium Occidentale L Para Indústria De Alimentos, , 113 f. Dissertação (Mestrado em Quimica)-Universidade Federal do Rio de Janeiro, Rio de Janeiro, 1999Buffo, R.A., Reineccius, G.A., Oehlert, G.W., Factors affecting the emulsifying and rheological properties of gum acacia in beverages emulsions (2001) Food Hydrocolloids, 15, pp. 53-66. , http://dx.doi.org/10.1016/S0268-005X(00)00050-3Cappi, A., de Jesus, S.S., Maciel, F., Secagem de α-amilase em spray dryer (2001) CONGRESSO INTERNO DE INICIAÇÃO CIENTIFICA DA UNICAMP, 9, p. 2001. , Campinas. Anais... Campinas: UnicampChang, Y.I., Scire, J., Jacobs, B., Effect of particle size and microstructure properties on encapsulated orange oil (1988) Flavor Encapsulation. Washington: American Chemical Society, pp. 87-102. , RISCH, S. J.REINECCIUS,G. A, cap. 10. (ACS Symposium Series 370Chattopadhyaya, S., Singhal, R.S., Kulkarn, P.R., Oxidized starch as gum Arabic substitute for encapsulation of flavours (1998) Carbohydrate Polymers, 37, p. 144. , http://dx.doi.org/10.1016/S0144-8617(98)00054-Xde Paula, R.C.M., Rodrigues, J.F., Composition and rheological properties of cashew tree gum, the exudates polysaccharide from Anacardium occidentale L (1995) Carbohydrate Polymers, 26, pp. 177-181. , http://dx.doi.org/10.1016/0144-8617(95)00006-Sde Jesus, S.S., (2002) Desenvolvimento E Analise Do Processo De Secagem De [alfa]-amilase Por Microondas a Vácuo, , 161 f. Dissertação (Mestrado em Engenharia Quimica)-Universidade Estadual de Campinas, Campinas, 2002Dziezak, J.D., A focus on gums. Building in texture, stability, and emulsification are just some of ways in which gums aid product development: Special Report (1991) Food Technology, 45 (3), pp. 116-132Filková, I., Mujumdar, A.S., (1995) Industrial Spray Drying Systems In Handbook of Industrial Drying, p. 742. , New York: Marcel Dekker IncGoubet, I., Quere, J.L., Voilley, A.J., Retention of aroma compounds by carbohydrates: Influence of their physicochemical characteristics and of their physical state. A review (1998) Journal Agricultural of Food Chemistry, 46, pp. 1981-1990. , http://dx.doi.org/10.1021/jf970709yInhamuns, A.J., Franco, M.R.B., EPA and DHA quantification in two species of freshwater fish from Central Amazonia (2008) Food Chemistry, 107, pp. 587-591. , http://dx.doi.org/10.1016/j.foodchem.2007.07.032Krabbendam, L., Relationship between DHA status at birth and child problem behaviour at 7 years of age. Prostaglandins (2007) Leukotrienes and Essential Fatty Acids, 76, pp. 29-34. , http://dx.doi.org/10.1016/j.plefa.2006.09.004, PMid:17074476Lamprecht, A., Schäfer, U., Lehr, C.M., Influences of process parameters on preparation of microparticle used as a carrier system for Ω-3 unsaturated fatty acid ethyl esters used in supplementary nutrition (2001) Journal of Microencapsulation, 18, pp. 347-357. , http://dx.doi.org/10.1080/02652040010000433, PMid:11308225Leite, J.T.C., (2001) Obtenção De Extrato De Inulina De Chicória (Cichorium Intybus) Por Abaixamento De Temperatura E Secagem Por Spray Dryer, , 120 f. Dissertação (Mestrado em Engenharia Agrícola)-Universidade Estadual de Campinas, Campinas, 2001Martin, C.A., Ácidos graxos poliinsaturados ômega-3 e ômega-6: Importância e ocorrência em alimentos (2006) Revista De Nutrição, 19 (6), pp. 761-770Narayan, B., Miyashita, K., Hosakawa, M., Physiological effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) - A review (2006) Food Reviews International, 22 (3), pp. 291-307. , http://dx.doi.org/10.1080/87559120600694622Nori, M.A., Ingredientes e aditivos microencapsulados na indústria de alimentos (1996) Engenharia De Alimentos, 1 (6), pp. 33-34Pauletti, M.S., Amestoy, P., Butter microencapsulation as affected by composition of wall material and fat (1999) Journal of Food Science, 64, pp. 279-282. , http://dx.doi.org/10.1111/j.1365-2621.1999.tb15882.xRé, M.I., Microencapsulation by spray drying (1998) Drying Technology, 16 (6), pp. 1195-1236. , http://dx.doi.org/10.1080/07373939808917460Ré, M.I., Microencapsulação: Em busca de produtos' inteligentes (2000) Ciência Hoje, 27 (162), pp. 24-29Robbers, J.E., Speedie, M.K., Tyler, V.E., (1997) Farmacognosia E Farmacobiotecnologia, p. 372. , Tradução de Benedetti, I. C. Supervisão Científica de Bastos, J. K. e outros. 1 a ed., Editorial Premier. São PauloRodrigues, R.A.F., (2004) Preparo, Caracterização E Avaliação Functional De Microcápsulas Obtidas Por Spray Drying, Contendo Extrato De Café Crioconcentrado, , 239 f. Tese (Doutorado em Engenharia de Alimentos)-Universidade Estadual de Campinas, Campinas, 2004Seaton, T., The Omega 3 Explosion (2006) Nutraceuticals World MagazineShahidi, F., Wanasundara, U.N., Omega 3-fatty acids: Nutritional aspects and production technologies (1998) Trends Food Science Technology, 9, pp. 230-240. , http://dx.doi.org/10.1016/S0924-2244(98)00044-2, PMid:21299575Shahidi, F., Han, X., Encapsulation of food ingredients (1993) Boca Raton, 33 (6), pp. 501-547. , http://dx.doi.org/10.1080/10408399309527645, Critical Review in Food Science and Nutrition PMid:8216812Simopoulos, A.P., Omega-3 fatty acids in wild plants, nuts and seeds (2002) Asia Pacific Journal of Clinical Nutrition, 11 (6), pp. 163-173. , http://dx.doi.org/10.1046/j.1440-6047.11.s.6.5.xUauy, R., Valenzuela, A., Marine oils: The health benefits of n-3 fatty acids (2000) Nutrition, 6 (7-8), pp. 680-684. , http://dx.doi.org/10.1016/S0899-9007(00)00326-9Wareing, M.V., (1997) Exudate Gums, pp. 87-118. , IMESON, A. Thickening and gelling agents for food. 2th ed. London: Blackie Academic & Professional, Chapman & HallWendel, S., Çelik, M., Uma visão geral sobre o uso da tecnologia de Spray-Drying (1998) Pharmaceutical Technology, pp. 31-45Zakaria, M.B., Rahman, Z.A., Rheological properties of cashew gum (1996) Carbohydrate Polymers, 29 (1), pp. 25-27. , http://dx.doi.org/10.1016/0144-8617(95)00132-

Synthesis, In Vitro Antiproliferative And Anti-mycobacterium Tuberculosis Activities Of Novel β-carboline Derivatives

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)A series of β-carboline derivatives with amino or guanidinium were synthesized and evaluated in vitro against anti-Mycobacterium tuberculosis and for antiproliferative activities against nine human cancer cell lines. The compounds 1-(4-hydroxyphenyl)-3-carboxamide(ethylamine) β-carboline (24.9 μg mL-1) and 1-(4-methoxyphenyl)-3-carboxamide(ethylamine) β-carboline (26.9 μg mL-1) were the most active against M. Tuberculosis (MTB). Compounds 1-(4-hydroxyphenyl)-3-carboxamide(ethylamine) β-carboline and 1-(4-methoxyphenyl)-3-carboxamide(propylamine) β-carboline, which had the same substituted groups, inhibited the growth of all human tumor cell lines with growth inhibitory activity (GI50) values from 1.37 to 9.20 mmol L-1. Also in this series, compounds 1-(4-hydroxyphenyl)-3-carboxamide(propylamine) β-carboline and 1-(3-nitrophenyl)-3-carboxamide(propylamine) β-carboline demonstrated significant activity against NCI/ADR cells. Among compounds with a terminal guanidine group, compounds 1-(4-hydroxyphenyl)-3-carboxamide(ethyl)guanidine β-carboline (27.8 μg mL-1) and 1-(3-nitrophenyl)-3-carboxamide(ethyl) guanidine β-carboline (37.4 μg mL-1) demonstrated the greatest activity against MTB. Additionally, compounds 1-(4-methoxyphenyl)-3-carboxamide(ethyl)guanidine β-carboline (GI50 = 0.45 mmol L-1) effectively inhibited growth and was highly selective against NCI/ADR. The in silico study revealed that 1-(4-hydroxyphenyl)-3-carboxamide(ethylamine) β-carboline, 1-(4-methoxyphenyl)-3-carboxamide(ethylamine) β-carboline, 1-(4-hydroxyphenyl)-3-carboxamide(propylamine) β-carboline, 1-(4-methoxyphenyl)-3-carboxamide(propylamine) β-carboline and 1-(3-nitrophenyl)-3-carboxamide(propylamine) β-carboline compounds follow the rules established by Lipinski, suggesting that this compound has no problems with oral bioavailability. © 2016 Sociedade Brasileira de Química.27813981405CAPES, Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorCNPq, Conselho Nacional de Desenvolvimento Científico e TecnológicoCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

In Vitro Activity Of Artemisia Annua L (asteraceae) Extracts Against Rhipicephalus (boophilus) Microplus [atividade In Vitro De Extratos De Artemisia Annua L (asteraceae) Sobre Rhipicephalus (boophilus) Microplus]

The activity of plant extracts on parasites may indicate groups of substances that are potentially useful for controlling Rhipicephalus (Boophilus) microplus. The aim of the present study was to investigate the in vitro action of Artemisia annua extracts on this tick. The concentrations of the sesquiterpene lactones artemisinin and deoxyartemisinin present in plant extracts were quantified via high-performance liquid chromatography. Four extracts produced from the concentrated crude extract (CCE) were evaluated on larvae using the impregnated paper method, with readings after 24 hours of incubation. The engorged females were immersed in the CCE and in its four derived extracts for five minutes, with incubation for subsequent analysis of biological parameters. The extracts were not effective on the larvae at the concentrations tested (3.1 to 50 mg.mL-1). The CCE showed greater efficacy on engorged females (EC50 of 130.6 mg.mL-1 and EC90 of 302.9 mg.mL-1) than did the derived extracts. These results tend to confirm that the action of artemisinin on engorged females of R. (B.) microplus is conditional to their blood intake. In this case, in vitro methods would be inadequate for effective evaluation of the action of A. annua on R. (B.) microplus.2013135Amaral, N.K., Guidelines for the evaluation of ixodicides against the cattle tick Boophilus microplus (Canestrini, 1887) (Acari: Ixodidae) (1993) Revista Brasileira De Parasitologia Veterinária, 2 (2), pp. 144-151(2009) Anuário Da Pecuária Brasileira, p. 360. , ANUALPEC, Sao Paulo: Angra FNP PesquisasBorges, L.M.F., Acao do extrato hexanico de frutos maduros de Melia azedarach (Meliaceae) sobre Boophilus microplus (Acari: Ixodidae) em bezerros infestados artificialmente (2005) Revista De Patologia Tropical, 34 (1), pp. 53-59Cala, A.C., (2010) Avaliação Da Atividade De Artemisia Annua L., Melia Azedarach L. E Trichilia Claussenii C. Sobre Nematódeos Gastrintestinais De Ovinos, p. 64. , Dissertacao (Mestrado)- Universidade Estadual Paulista, Faculdade de Ciencias Agrarias e Veterinarias, JaboticabalCeleghini, R.M.S., Desenvolvimento e validacao de metodologia analitica por CLAE-IR para determinacao de artemisinina em Artemisia annua L (2009) Química Nova, 32 (4), pp. 875-878Chagas, A.C.S., Efeito acaricida de oleos essenciais e concentrados emulsionaveis de Eucalyptus em Boophilus microplus (2002) Brazilian Journal of Veterinary Research and Animal Science, 39 (5), pp. 247-253Chagas, A.C.S., Sensibilidade do carrapato Boophilus microplus a solventes (2003) Ciência Rural, 33 (1), pp. 109-114Creek, D.J., Kinetics of ironmediated artemisinin degradation:Effect of solvent composition and iron salt (2005) Journal of Pharmaceutical Sciences, 94 (8), pp. 1820-1829Drummond, R.O., Boophilus annulatus and B. Microplus: Laboratory tests of insecticides (1973) Journal of Economic Entomology, 66 (1), pp. 130-133Ekanem, A.P., Brisibe, E.A., Effects of ethanol extract of Artemisia annua L. Against monogenean parasites of Heterobranchus longifilis (2010) Parasitology Research, 106 (5), pp. 1135-1139Fernandes, F.F., Freitas, E.P.S., Acaricidal activity of an oleoresinous extract from Copaifera reticulata (Leguminosae: Caesalpinioideae against larvae of the southern cattle tick, Riphicephalus (Boophilus) microplus (Acari:Ixodidae) (2007) Veterinary Parasitology, 147 (1-2), pp. 150-154Ferreira, J.F.S., Agrotechnological aspects of the anti-malarial plant Artemisia annua and its potential use in animal health in Appalachia (2006) International Symposium on Perfume, Aromatic and Medicinal Plants, (2-4), pp. 797-804. , Revue de Regions Arides - Numero special - Actes du seminaire international les Plants a Parfum, Aromatiques et Medicinales (English version: International Center for Agricultural Research in Dry AreasFerreira, J.F.S., Nutrient Deficiency in the Production of Artemisinin, Dihydroartemisinic Acid, and Artemisinic Acid in Artemisia annua L (2007) Journal of Agricultural and Food Chemistry, 55 (5), pp. 1686-1694Ferreira, J.F.S., Gonzalez, J.M., Chemical and biological stability of artemisinin in bovine rumen fluid and its kinetics in goats (Capra hircus) (2008) Revista Brasileira De Parasitologia Veterinária, 17 (SUPPL. 1), pp. 103-109Furlong, J., O carrapato dos bovinos e a resistencia: Temos o que comemorar? (2007) A Hora Veterinária, 159, pp. 26-32Grisi, L., Impacto economico das principais ectoparasitoses em bovinos no Brasil (2002) A Hora Veterinária, 21 (125), pp. 8-10Heimerdinger, A., Extrato alcoolico de Capim-cidreira (Cymbopogon citratus) no controle do Boophilus microplus em bovinos (2006) Revista Brasileira De Parasitologia Veterinária, 15 (1), pp. 37-39Idris, U.A., Adam, S.E., Tartour, G., The anthelmintic efficacy of Artemisia herba-alba against Haemonchus contortus infection in goats (1982) Animal Health Quarterly, 22 (3), pp. 138-143Iqbal, Z., Anthelmintic activity of Artemisia brevifolia in sheep (2004) Journal of Ethnopharmacology, 93 (2), pp. 265-268Keiser, J., Artesunate and artemether are effective fasciolicides in the rat model and in vitro (2006) Journal of Antimicrobial Chemotherapy, 57 (6), pp. 1139-1145Keiser, J., Efficacy and safety of artemether against a natural Fasciola hepatica infection in sheep (2008) Parasitology Research, 103 (3), pp. 517-522Keiser, J., Utzinger, J., Food-borne trematodiasis: Current chemotherapy and advances with artemisinins and synthetic trioxolanes (2007) Trends In Parasitology, 23 (11), pp. 555-562Klayman, D.L., Qinghaosu (Artemisinin): Na antimalarial drug from China (1985) Science, 228 (4703), pp. 1049-1055La-Scalea, M.A., Silva, H.S.R.C., Ferreira, E., Reducao voltametrica de artemisinina e sua interacao com grupo heme (hemina) (2007) Brazilian Journal of Pharmaceutical Sciences, 43 (3), pp. 371-383Martins, R.M., Estudio in vitro de la accion acaricida del aceite esencial de la graminea Citronela de Java (Cymbopogon winterianus Jowitt) en la garrapata Boophilus microplus (2006) Revista Brasileira De Plantas Medicinais De Botucatu, 8 (2), pp. 71-78Meshnick, S.R., Second-generation antimamalarial endoperoxides (1996) Parasitology Today, 12 (2), pp. 79-82Ribeiro, V.L.S., Acaricidal properties of extracts from the aerial parts of Hypericum polyanthemum on the cattle tick Boophilus microplus (2007) Veterinary Parasitology, 147 (1-2), pp. 199-203Rodrigues, R.A.F., Otimizacao do processo de extracao e isolamento do antimalarico artemisinina a partir de Artemisia annua L (2006) Química Nova, 29 (2), pp. 368-372Silva, H.S.R.C., (2006) Antimaláricos Potenciais: Pró-fármacos Poliméricos E Formas De Liberação Controlada De Artemisinina, p. 209. , Tese (Doutorado)-Universidade de Sao Paulo, Sao PauloSimoes, C.M., (2004) Farmacognosia: Da Planta Ao Medicamento, p. 1102. , 5. ed. Porto Alegre: Editora da UFRGSFlorianopolis: Editora da UFSCTaranto, A.G., Estudo sobre o mecanismo de acao da artemisinina e dos endoperoxidos, a mais nova classe de agents antimalaricos (2006) Sitientibus, 34, pp. 47-58Tariq, K.A., Anthelmintic activity of extracts of Artemisia absinthium against ovine nematodes (2009) Veterinary Parasitology, 160 (1-2), pp. 83-8

Fano resonance in electronic transport through a quantum wire with a side-coupled quantum dot: X-boson treatment

The transport through a quantum wire with a side coupled quantum dot is studied. We use the X-boson treatment for the Anderson single impurity model in the limit of $U=\infty$. The conductance presents a minimum for values of T=0 in the crossover from mixed-valence to Kondo regime due to a destructive interference between the ballistic channel associated with the quantum wire and the quantum dot channel. We obtain the experimentally studied Fano behavior of the resonance. The conductance as a function of temperature exhibits a logarithmic and universal behavior, that agrees with recent experimental results.Comment: 6 pages, 10 eps figs., revtex

Synthesis And Evaluation Of New β-carboline-3-(4-benzylidene)- 4h-oxazol-5-one Derivatives As Antitumor Agents

In the present work, we report the synthesis and in vitro anticancer and antimicrobial activity evaluation of a new series of 1-substituted-β- carboline derivatives bearing a 4-benzylidene-4H-oxazol-5-one unity at C-3. The compound 2-[1-(4- methoxyphenyl)-9H-β-carbolin-3-yl]-4-(benzylidene)-4H- oxazol-5-one (11) was the most active derivative, exhibiting a potent cytotoxic activity against glioma (U251), prostate (PC-3) and ovarian (OVCAR-03) cancer cell lines with IC 50 values of 0.48, 1.50 and 1.07 μM, respectively. An in silico study of the ADME properties of the novel synthesized β-carboline derivatives was also performed. © 2012 by the Authors.17561006113Cao, R., Peng, W., Wang, Z., Xu, A., B-carboline alkaloids: Biochemical and pharmacological functions (2007) Curr. Med. 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Correction: Caryocar Brasiliense Supercritical Co2 Extract Possesses Antimicrobial And Antioxidant Properties Useful For Personal Care Products [bmc Complement Altern Med, 14, 73, (2014)]

[No abstract available]14Lilian, F.B.A., Patricia, M., Mary Ann, F., Priscila, G.M., Caryocar brasiliense supercritical CO2 extract possesses antimicrobial and antioxidant properties useful for personal care products (2014) BMC Complement Altern Med, 14, p. 73. , 10.1186/1472-6882-14-73, 3940022, 2456530