Ultrastructural Changes and Bacterial Localization in Buffalo Calves Following Oral Exposure to Pasteurella multocida B:2

This study describes the ultrastructural changes and distribution of P. multocida B:2 in the respiratory and gastrointestinal tracts of buffalo calves following oral exposure and compared with intratracheal exposure. Nine 8-month old buffalo calves were divided into 3 groups. At the start of the experiment, dexamethasone (1mg/kg) was injected intramuscularly into buffaloes of all groups for three consecutive days. Then, calves of Group 1 were exposed orally to 50ml of the inoculum containing 109 colony-forming unit (CFU)/mL of live P. multocida B:2. Calves of Group 2 were exposed intra-tracheal to the same inoculum while calves of Group 3 were exposed orally to PBS. Transient and mild clinical signs were observed in calves of Groups 1 and 2. Pasteurella multocida B:2 was isolated from the nasal mucosa for up to 5 days post-infection. Only calf that was killed at 48 h post-infection had P. multocida B:2 in both respiratory and gastro-intestinal tracts showing ultrastructural changes typical of acute cellular injury, with degeneration of endothelium and vascular walls. There were deciliation in the respiratory and microvilli degeneration in the gastrointestinal tracts. The study concludes that oral exposure may not play major role in the development of hemorrhagic septicemia. Nevertheless, the buffalo calf that succumbed to hemorrhagic septicemia carried P. multocida B:2 in the gastro-intestinal organs

Disease Conditions in Farm and Smallholder Buffaloes of Serdang, Malaysia

Analysis of records revealed that there were no significant differences in the number of disease Conditions between buffaloes of the smallholders and Universiti Pertanian Malaysia (UPM) farm, and between the calves and adult buffaloes of both groups. Significant differences were, however, observed on the type of disease conditions involved. The differences were probably associated with the system of management

Growth and reproductive performances of farmed timorensis deer, Cervus timorensis

A retrospective study was conducted to evaluate the growth and reproductive performances of timorensis deer, Cervus timorensis, that were kept in farm. All 140 animals were allowed to graze at all times while supplemented feed of approximately 1 kg/animal/day was provided except during the rainy season between October and December when supplementation was 2 kg/animal/day. Available farm records between 2011 and 2014 were collected and analysed for growth and reproductive performances. Most fawning occurred between October and December (average rainfall 413 mm) with an apparent peak in December. Average annual fawning percentage for the past four years was 57% while the average rate of mortality for fawns and adults was 5.4% and 13.5%, respectively. The average daily weight gain for up to one year of age was 0.042±0.02 kg but after one year it was 0.052±0.01 kg. The average body weight of adult timorensis at 24 months old was 48.5±1.4 and 44.5±0.9 kg for male and female, respectively. Monthly rainfall showed significant (p<0.05) negative correlation with the ADG but showed positive correlation (p<0.05) with fawning. Body weight gain was high during the dry months of May and August (average rainfall 150 mm). There seemed to be a clear breeding season for timorensis deer in this study, which was within the moderate months of March to April (average rainfall 236 mm) when body weight started to increase

Experimental infection of goats by Pasteurella multocida B:2

An experiment was carried out to determine whether goats can be infected by Pasteurella multocida B:2, the causative agent of haemorrhagic septicaemia of cattle and buffaloes. Thirty-six healthy local goats were divided into four groups consisting of nine goats per group. Goats of groups 1 and 3 were inoculated intranasally with 1ml inoculum containing 109 CFU of live P. multocida B:2. Goats of groups 2 and 4 were not infected, but were exposed to the infected animals by keeping goats of group 2 together with group 1 while group 4 together with group 3. Immediately post-infection, goats in groups 3 and 4 were treated with dexamethasone at the rate of 1mg/kg for three consecutive days. Three goats from each of the four groups were killed on days 7,14 and 21 post-infection. One goat from group 3 died percutely on day 2 post-infection, showing lesions typical ofhaemorrhagic septicaemia. Other two goats from the same group were weak. None of the goats in groups 1, 2 and 4 succumbed to the disease but most exhibited signs of mild respiratory tract infection. Post-mortem examinations revealed that 20 (53 %) goats had mild, acute pneumonia affecting less than 7% of the lung, suggesting that P. multocida B:2 is not a primary pathogen of the respiratory tract. Four (11 %) goats of group 3 had pulmonary oedema and congestion, indicating that dexamethasone enhanced chances of goats being infected by P. multocida B:2. P. multocida was successfully re-isolated from lungs and nasal swabs of goats from all groups, and heart blood of goats from group 3 but not from the lymph nodes and tonsils. The re-isolation of P. multocida B:2 was successfully made for up to 14 days p.i, in groups without dexamethasone but for up to 21 days following dexamethasone treatment. Transmission of P. multocida B:2 to the in-contact goats occurred at a rate of 40%

Seroprevalence and detection of contagious bovine pleuropneumonia (CBPP) in northeast states of Peninsular Malaysia

Contagious Bovine Pleuropneumonia (CBPP) is a highly infectious disease of cattle caused by Mycoplasma mycoides subsp. mycoides Small Colony (MmmSC). It is a disease recognised by OIE that needs to be controlled or eradicated through surveillance system. This study establishes the sero-prevalence of CBPP, as well as attempts to isolate and identify the agent of CBPP from cattle in Kelantan and Terengganu, the northeastern states of Peninsular Malaysia, where cattle movement was high. A total of 3,242 sera from 428 farms were processed between 2011 and 2014 using the competitive ELISA (c-ELISA). The animal-level prevalence ranged between 5% (46/917) in Terengganu and 9% (220/2325) in Kelantan, whereas the herd prevalence ranged between 12% (22/210) in Terengganu and 24% (53/218) in Kelantan. The overall animal-level prevalence was 8% (266/3242), while the herd prevalence was 17% (75/428). Two hundred and four nasal swabs from 18 positive herds in Kelantan and 163 lung and mediastinal lymph node samples were processed for isolation of MmmSc before confirmation by PCR and immunoperoxidase. Forty-one samples showed turbidity in PPLO broth and 15 lung and mediastinal lymph node samples had 'fried-egg colony' growth on the PPLO agar, which were suggestive of Mycoplasma infection. However, all were negative for MmmSC by PCR and immunoperoxidase. Thus, the northeastern part of Peninsular Malaysia remains positive serology for CBPP without isolation

Ultrastructural pathology of the upper respiratory tract of rabbits experimentally infected with Pasteurella multocida A:3

Twenty-four 8 to 9 week-old Pasteurella multocida-free rabbits were divided into three equal groups, the first group was pretreated with hydrocortisone and inoculated intranasally with pasteurella multocida serotype A:3. The second group was inoculated intranasally with P. multocida without hydrocortisone treatment. The third group was inoculated with phosphate buffered saline only and used as a control group. Pasteurella multocida was isolated from the nasal cavity of all infected rabbits in group 1 and 2 and from the trachea of seven rabbits in group 1 and five rabbits in group 2. This study was conducted to observe the ultrastructural changes of the upper respiratory tract of hydrocortisone treated and non-treated rabbits infected with P. multocida serotype A:3. The ultrastructural changes detected in infected rabbits were ciliary destruction and deciliation of the ciliated epithelial cells, cellular swelling, goblet cell hyperplasia and endothelial cell damage. Pasteurella multocida was observed attached to the degenerated cilia,microvilli and mucus. Pasteurella multocida infection was associated with inflammatory responses, which may have caused tissue damage. It is possible that hydrocortisone modulates the severity of infection as an immune suppressor and an inhibitor of goblet cell secretion

Water Thermocline Confirms Susceptibility of Tilapia Cultured in Lakes to Streptococcus agalactiae

A study was conducted on water quality profiling to confirm susceptibility of tilapia cultured in lakes to Streptococcus agalactiae infection. A total of 1,010 and 719 tilapias of different sizes were collected from two lakes; the Kenyir and Pedu lakes, respectively. They were randomly sampled for a period of 24 months. Swabs of brain, eye and kidney were streaked directly onto blood agar before S. agalactiae was identified by the API 20 STREP kit, Slidex Strepto-kit and PCR technique. The water temperature (thermocline) and dissolved oxygen profiling were determined at 1 m intervals for up to 20 m deep. Water clarity and flow rate were also recorded using Secchi disk and a current meter. S. agalactiae was successfully isolated from both lakes throughout the year, ranging between 2 and 78%. Isolation was more frequent during the hot and dry months of both years. During this period, the mean water temperature was >29 degrees C for up to 8 m deep due to the significantly (p12 m deep. This and the slow water flow kept the water temperature at 4 m deep where tilapias under the cage culture system were kept to remain high causing stress to tilapia and increases susceptibility to S. agalactiae. Dissolved oxygen profiling, however remained high at >5 mg L-1 for up to 8 m deep and did not give adverse effects to susceptibility of tilapia to S. agalactiae

Water Thermocline Confirms Susceptibility of Tilapia Cultured in Lakes to Streptococcus agalactiae

A study was conducted on water quality profiling to confirm susceptibility of tilapia cultured in lakes to Streptococcus agalactiae infection. A total of 1,010 and 719 tilapias of different sizes were collected from two lakes; the Kenyir and Pedu lakes, respectively. They were randomly sampled for a period of 24 months. Swabs of brain, eye and kidney were streaked directly onto blood agar before S. agalactiae was identified by the API 20 STREP kit, Slidex Strepto-kit and PCR technique. The water temperature (thermocline) and dissolved oxygen profiling were determined at 1 m intervals for up to 20 m deep. Water clarity and flow rate were also recorded using Secchi disk and a current meter. S. agalactiae was successfully isolated from both lakes throughout the year, ranging between 2 and 78%. Isolation was more frequent during the hot and dry months of both years. During this period, the mean water temperature was >29 degrees C for up to 8 m deep due to the significantly (p12 m deep. This and the slow water flow kept the water temperature at 4 m deep where tilapias under the cage culture system were kept to remain high causing stress to tilapia and increases susceptibility to S. agalactiae. Dissolved oxygen profiling, however remained high at >5 mg L-1 for up to 8 m deep and did not give adverse effects to susceptibility of tilapia to S. agalactiae

Clinical, biochemical and histological changes during developement of pregnancy ketosis in goats

Thin does or very over-conditioned does and does carrying with multiple pregnancies are more likely to develop pregnancy ketosis (pregnancy toxaemia). Pregnancy ketosis is one of the metabolic disorders in ruminant animal such as does and ewes during the last weeks of pregnancy (5 – 6 weeks before gestation) and beside high ketone body levels characterised by free fatty acids levels (FFA). These FFA are derived from the adipose tissue. The ability of adipocytes to respond to changes in the energy balance, by secretion of FFA makes this parameter a main indicator for disorder that coincide with changes in energy balance. It is biochemically and endocrinologically characterized by ketosis, hypoinsulinemia and an elevation of B- hydroxybtyrate concentration in the plasma, free fatty acids (FFA), and cortisol. However, the effect of plasma free fatty acid on pathophysiology, metabolites and histological changes of this disorder remain poorly understood. Clinical signs, often with a slow onset, are characterised by neuorological signs like teeth grinding, stress and dull eyes. Ketosis also affected on pathological part which characterized by fatty liver. In this review, we are focusing various key aspects of the disease with special reference on the clinical, biochemical and histological changes during development of pregnancy ketosis in goat population

Immunohistochemical evaluation of lesions in the gastrointestinal tract of buffalo (Bubalus bubalis) calves orally exposed to Pasteurella multocida B:2

The gastrointestinal lesions and bacterial distribution of buffalo calves were evaluated histologically using immunoperoxidase, following oral exposure to wild-type Pasteurella multocida B:2 at 109cfu/mL in   phosphate buffered saline. The lesions were basically of mild to severe mucohaemorrhagic abomasitis and  enteritis.The lesions were confirmed to be associated with the inoculated P. multocida B:2, using the   immunoperoxidase technique. P. multocida B:2 antigen was detected not only in the bacterial clusters in  the gastric pits, intestinal epithelia and capillaries, Brünner’s glands and Crypt of Lieberkühn but was also  seen interacting with infiltrating neutrophils and macrophages intracellularly and on the surface of  erythrocyte in congested vessels and haemorrhages. We observed higher localization and distribution of the  immunoperoxidase reaction with increased severity of lesions along the gastrointestinal tract. This suggest intensity increases with increased amount of P. multocida B:2 or antigen in the tissue, which  possibly leads to increase tissue damage.Keywords: Buffalo calves, Gastrointestinal lesions, Immunoperoxidase, Oral infection, Pasteurella  multocida B: