MORPHOFUNCTIONAL PECULIARITIES OF MUCOSAL IMMUNITY DEPENDING ON METHODS OF OPPORTUNISTIC MICROBIAL INTRODUCTION

Immunophenotypic and morphological peculiarities of immune reactions under intranasal, peroral and subcutaneous introduction of multicomponent vaccine Immunovac VP-4 containing a group of opportunistic bacterial antigens were investigated. The investigated preparation was found to cause marked activation of congenital immunity effectors both in parenteral and mucosal immunization. It is manifested in the expression of differential, costimulatory, adhesive molecules on the surface of mononuclear leukocytes in proliferation of key mucosal immunity effectors (γδТ, В1, NK cells), and changes in structure, cellular composition of immunocompetent organs both regional and distant as to the site of introduction. Currently existing data on effects and mechanisms of vaccine and immunomodulator mucosal introduction allows considering the development of mucosal mono- and associated vaccines as the priority direction in modern vaccinology

Development of potential therapeutic approach for correction of dermatitis of different nature

The aim of the study – to develop a targeted delivery method for correction of skin barrier disorders using an adapted algorithm to recruit additional copies of the Klotho gene into target cells and a method to validate its overexpression.Цель исследования – разработка метода таргетной доставки для коррекции нарушений барьеров кожи с применением ранее разработанного алгоритма, позволяющего вызвать гиперэкспрессию Klotho в клетках- мишенях

New heterocyclic compounds of the azolopyrimidine series with pronounced antitumor activity

The purpose of the study was to study the effect of new azolopyrimidine compounds on the viability of cultured tumor cells and to identify promising compounds for the development of new antitumor drugs.Цель исследования – изучение влияния новых азолопиримидиновых соединений на жизнеспособность культивируемых опухолевых клеток и выявление перспективных соединений для разработки новых противоопухолевых препаратов

ВОССТАНОВЛЕНИЕ ФУНКЦИЙ ВНЕШНЕГО ДЫХАНИЯ У ПОСЛЕОПЕРАЦИОННЫХ ПАЦИЕНТОВ С ПОМОЩЬЮ ТЕХНОЛОГИИ БИОУПРАВЛЕНИЯ, ОРГАНИЗОВАННОГО ПО КОНЦЕНТРАЦИИ УГЛЕКИСЛОГО ГАЗА В ВЫДЫХАЕМОМ ВОЗДУХЕ И ЧАСТОТЕ ДЫХАНИЯ

The methodical, technical and software realization of the biofeedback of expiration parameters are described. The biofeedback training method based on the breath frequency and CO2  concentration regulation is proposed. Preliminary study was conducted in the group of patients after abdominal surgical. It has been shown that the patients master their skills of CO2  concentration self-regulation. Using biofeedback for the postsurgical rehabilitation facilitates the rapid recovery of the expiration parameters.В статье рассматриваются методические, технические и программные аспекты адаптивного биоуправления, организованного по параметрам внешнего дыхания. Предложена методика БОС-тренинга, основанная на регуляции частоты дыхания и содержания углекислого газа в выдыхаемом воздухе. Проведены предварительные исследования в группе пациентов после абдоминальной хирургической операции. Показано, что пациенты осваивают навыки произвольной регуляции содержания углекислого газа в выдыхаемом воздухе. Был сделал вывод о том, что применение БОС-тренинга в послеоперационном периоде способствует быстрому восстановлению параметров внешнего дыхания

CK2 Inhibition and Antitumor Activity of 4,7-Dihydro-6-nitroazolo[1,5-a]pyrimidines

Today, cancer is one of the most widespread and dangerous human diseases with a high mortality rate. Nevertheless, the search and application of new low-toxic and effective drugs, combined with the timely diagnosis of diseases, makes it possible to cure most types of tumors at an early stage. In this work, the range of new polysubstituted 4,7-dihydro-6-nitroazolo[1,5-a]pyrimidines was extended. The structure of all the obtained compounds was confirmed by the data of 1H, 13C NMR spectroscopy, IR spectroscopy, and elemental analysis. These compounds were evaluated against human recombinant CK2 using the ADP-GloTM assay. In addition, the IC50 parameters were calculated based on the results of the MTT test against glioblastoma (A-172), embryonic rhabdomyosarcoma (Rd), osteosarcoma (Hos), and human embryonic kidney (Hek-293) cells. Compounds 5f, 5h, and 5k showed a CK2 inhibitory activity close to the reference molecule (staurosporine). The most potential compound in the MTT test was 5m with an IC50 from 13 to 27 µM. Thus, our results demonstrate that 4,7-dihydro-6-nitroazolo[1,5-a]pyrimidines are promising for further investigation of their antitumor properties. © 2022 by the authors.Ministry of Education and Science of the Russian Federation, Minobrnauka: FEUZ-2020–0058, H687.42B.223/20This work was financially supported by the Ministry of Science and Higher Education of the Russian Federation, State Contract № FEUZ-2020–0058 (H687.42B.223/20)

Investigation of cytotoxic effect of new mono- and as-triazines on malignantly degenerated cells

The purpose of the study is to determinate the effectiveness of the antitumor effect of new acridone and xanthone derivatives on tumor cell lines.Цель исследования – определение эффективности противоопухолевого действия исследуемых аналогов акридонов и ксантонов на опухолевые клеточные линии

Effect of new cycloplatinated complexes on the viability of cultured human glioblastoma and osteosarcoma cells

The article presents the results of studies conducted on cell cultures of glioblastoma and osteosarcoma (lines A-172 and HOS). The effect of new platinum compounds on the viability of cultured cells was studied. As a result, a visible cytotoxic effect was observed.В статье представлены результаты исследований, которые были проведены на клеточных культурах глиобластомы и остеосаркомы (линии А-172 и HOS). Исследовалось влияние новых соединений платины на жизнеспособность культивируемых клеток. В результате был отмечен видимый цитотоксический эффек

Antiretroviral Therapy Initiation Before, During, or After Pregnancy in HIV-1-Infected Women: Maternal Virologic, Immunologic, and Clinical Response

Pregnancy has been associated with a decreased risk of HIV disease progression in the highly active antiretroviral therapy (HAART) era. The effect of timing of HAART initiation relative to pregnancy on maternal virologic, immunologic and clinical outcomes has not been assessed.We conducted a retrospective cohort study from 1997–2005 among 112 pregnant HIV-infected women who started HAART before (N = 12), during (N = 70) or after pregnancy (N = 30).0.01). There were no statistical differences in rates of HIV disease progression between groups.HAART initiation during pregnancy was associated with better immunologic and virologic responses than initiation after pregnancy

INFORMATION-ANALYTICAL MODEL FOR THE SELECTION OF MANAGEMENT METHODS ACCORDING TO THE DEGREE OF THEIR INFLUENCE ON EFFICIENCY OF PRODUCTION ACTIVITY OF CONSTRUCTION ORGANISATIONS

Objectives. Relevant problems related to the adoption of unprogrammed solutions in the process of managing the production  activity of a construction enterprise in unstable, a priori  underdetermined economic environments are addressed.The aim of  the study is todevelop tools and methods for assessing the  effectiveness of the current state of construction production and on  this basis to identify the most effective management of a  construction organisation under unstable market conditions.Methods. The methodology of the conducted research is based on the application of the mathematical apparatus of fuzzy sets for the  processing of expert data and the formation on this basis of linguistic functions that determine the analytical relationship between various  indicators of the production activity of the construction organisation.Results. A method is developed for processing expert data using  fuzzy logic, making it possible to form an analytical relationship  between the performance indicators of the production activity of the  construction organisation and the management parameters that  determine its current state. An alternative means for selecting the  most effective management approach, accounting for its influence on the efficiency of the production activity of the construction organisation, is developed.Conclusion. An information-economic model of situational management of the development of a construction organisation is  developed. The proposed tools and methods allow the effective  management of the production activity of the construction  organisation to be organised, and on this basis to increase the  efficiency of production under different economic conditions in the presence of spontaneously arising perturbative factors. The methodical approach to the organisation of management of the  production activity efficiency of the construction organisation allows the effective subsystems of management of other activities of  the construction organisation concerning the rejection and  indignation in the unstable conditions of the modern market  environment to be formed without fundamental changes in the organisational structure