4 research outputs found
Взаимосвязь показателей эритроцитарного звена гемостаза со структурно-функциональными параметрами сердца у больных гипертензивной энцефалопатией
Investigation aim is to expose sex features of interdependence between erythrocytes homeostasis indices and heart remodeling in essential hypertension (EH) and hypertensive encephalopathy (HE) patients. 168 patients (110 men) with EH 2th staid, 2th degree and HE 1—2th staid were enrolled. It was executed blood test, echocardiography by standard method. In men erythrocytes homeostasis indices correlated with structural and functional alterations. In women erythrocytes homeostasis indices correlated with diastolic function. There were exposed sex-specific interdependence between erythrocytes homeostasis indices and heart remodeling.Исследованы половые особенности взаимосвязи показателей эритроцитарного гомеостаза с ремоделированием сердца у больных гипертонической болезнью (ГБ) и гипертензивной энцефалопатией (ГЭ).Обследовано 168 больных (110 мужчин, 58 женщин) ГБ II стадии II степени, осложненной ГЭ 1—2-й стадии. Проводились общеклиническое исследование крови, эхокардиография по стандартной методике.У мужчин показатели эритроцитарного гомеостаза коррелировали со структурно-функциональными изменениями, у женщин — с диастолической функцией.Выявлены пол-специфичные взаимосвязи эритроцитарного гомеостаза с ремоделированием сердца
A new class of glycomimetic drugs to prevent free fatty acid-induced endothelial dysfunction
Background: Carbohydrates play a major role in cell signaling in many biological processes. We have developed a set of glycomimetic drugs that mimic the structure of carbohydrates and represent a novel source of therapeutics for endothelial dysfunction, a key initiating factor in cardiovascular complications. Purpose: Our objective was to determine the protective effects of small molecule glycomimetics against free fatty acidinduced endothelial dysfunction, focusing on nitric oxide (NO) and oxidative stress pathways. Methods: Four glycomimetics were synthesized by the stepwise transformation of 2,5dihydroxybenzoic acid to a range of 2,5substituted benzoic acid derivatives, incorporating the key sulfate groups to mimic the interactions of heparan sulfate. Endothelial function was assessed using acetylcholineinduced, endotheliumdependent relaxation in mouse thoracic aortic rings using wire myography. Human umbilical vein endothelial cell (HUVEC) behavior was evaluated in the presence or absence of the free fatty acid, palmitate, with or without glycomimetics (1µM). DAF2 and H2DCFDA assays were used to determine nitric oxide (NO) and reactive oxygen species (ROS) production, respectively. Lipid peroxidation colorimetric and antioxidant enzyme activity assays were also carried out. RTPCR and western blotting were utilized to measure Akt, eNOS, Nrf2, NQO1 and HO1 expression. Results: Ex vivo endotheliumdependent relaxation was significantly improved by the glycomimetics under palmitateinduced oxidative stress. In vitro studies showed that the glycomimetics protected HUVECs against the palmitateinduced oxidative stress and enhanced NO production. We demonstrate that the protective effects of preincubation with glycomimetics occurred via upregulation of Akt/eNOS signaling, activation of the Nrf2/ARE pathway, and suppression of ROSinduced lipid peroxidation. Conclusion: We have developed a novel set of small molecule glycomimetics that protect against free fatty acidinduced endothelial dysfunction and thus, represent a new category of therapeutic drugs to target endothelial damage, the first line of defense against cardiovascular disease
Interdependence between erythrocytes homeostasis indices and structural and functional heart parameters in hypertensive encephalopathy patients
Investigation aim is to expose sex features of interdependence between erythrocytes homeostasis indices and heart remodeling in essential hypertension (EH) and hypertensive encephalopathy (HE) patients. 168 patients (110 men) with EH 2th staid, 2th degree and HE 1—2th staid were enrolled. It was executed blood test, echocardiography by standard method. In men erythrocytes homeostasis indices correlated with structural and functional alterations. In women erythrocytes homeostasis indices correlated with diastolic function. There were exposed sex-specific interdependence between erythrocytes homeostasis indices and heart remodeling