46 research outputs found
Effect of urokinase gene knockout on tissue levels of biogenic amines in mice with melanoma
The research aim was to study the dynamics of biogenic amines in the brain, tumor and intact skin of urokinase (uPA) gene knockout mice on day 21 of the B16/F10 melanoma growth.Material and methods. The study included male and female uPA gene knockout (-uPA, n = 38) and wild type mice (+uPA, n = 61). Melanoma was transplanted subcutaneously. Levels of biogenic amines were studied by ELISA in tissues obtained on day 21 of carcinogenesis.Results and discussion. Intact (-uPA) mice showed an increased total content of biogenic amines: in the skin - due to noradrenaline increase by 4.8 times in males and by 4.9 times in females, histamine - by 3.6 times in males and by 1.6 times (p < 0.05) in females, serotonin - by 3.4 times in males and by 8.3 times in females; in the brain - due to noradrenaline increase by 3.5 times in males and by 3.2 times in females, dopamine by 2.1 times in males and by 2.9 times in females, while histamine content decreased. Melanoma development in (-uPA) mice was characterized by: lower levels of adrenaline with high NA concentrations and an increase in the serotonin metabolism in the brain; higher histamine concentrations in the tumor and higher serotonin levels in the skin; similar to (+uPA) mice levels of adrenaline (males) and noradrenaline in the tumor and higher levels of adrenaline in the tumor and histamine in the skin in (-uPA) females.Conclusions. The uPA gene knockout limits the development of stress at the central regulatory level due to lower levels of A together with increasing serotoninergic mediation in the brain, as well as modulates the immune antitumor response due to higher levels of histamine in the tumor and 5 serotonin in the skin, as a result of lower monoamine oxidase activity, in mice with B16/F10 melanoma
ΠΠΎΠ»ΡΡΠ΅Π½ΠΈΠ΅ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ½ΡΡ ΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΠΎΠ² ΡΠΎ ΡΠ»ΠΎΠΈΡΡΡΠΌΠΈ ΡΠΈΠ»ΠΈΠΊΠ°ΡΠ°ΠΌΠΈ ΠΈΠ· Π»Π°ΡΠ΅ΠΊΡΠ° Π½Π°ΡΡΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΊΠ°ΡΡΡΠΊΠ°
The structure and mechanical properties of polymer films obtained from natural latex and filled by clay suspensions were investigated. It was shown that surface modification of the clay particles by alkylbenzyldimethylammonium chloride decrease film properties. Mixing of latex with untreated bentonite lead to the inorganic layers reagregation and dehydratation at the drying stage but supports high mechanical properties due to parallel orientation of the clay particles.ΠΠ·ΡΡΠ΅Π½Π° ΡΡΡΡΠΊΡΡΡΠ° ΠΈ ΡΠΈΠ·ΠΈΠΊΠΎ-ΠΌΠ΅Ρ
Π°Π½ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π° ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ½ΡΡ
ΠΏΠ»Π΅Π½ΠΎΠΊ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π½Π°ΡΡΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΊΠ°ΡΡΡΠΊΠ° Β«Π Π΅Π²ΡΠ»ΡΠ΅ΠΊΡΒ», Π½Π°ΠΏΠΎΠ»Π½Π΅Π½Π½ΡΡ
ΡΠ»ΠΎΠΈΡΡΡΠΌΠΈ ΡΠΈΠ»ΠΈΠΊΠ°ΡΠ°ΠΌΠΈ Π½Π° ΡΡΠ°Π΄ΠΈΠΈ Π»Π°ΡΠ΅ΠΊΡΠ°. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΏΡΠΈ Π²Π²Π΅Π΄Π΅Π½ΠΈΠΈ Π² Π»Π°ΡΠ΅ΠΊΡ Π½Π΅ΠΎΠ±ΡΠ°Π±ΠΎΡΠ°Π½Π½ΠΎΠ³ΠΎ ΡΠ»ΠΎΠΈΡΡΠΎΠ³ΠΎ ΡΠΈΠ»ΠΈΠΊΠ°ΡΠ° ΠΏΡΠΎΠΈΡΡ
ΠΎΠ΄ΠΈΡ Π°Π³ΡΠ΅Π³Π°ΡΠΈΡ ΡΠ°ΡΡΠΈΡ, Π΄Π΅Π³ΠΈΠ΄ΡΠ°ΡΠ°ΡΠΈΡ ΠΌΠ΅ΠΆΡΠ»ΠΎΠ΅Π²ΠΎΠ³ΠΎ ΠΏΡΠΎΡΡΡΠ°Π½ΡΡΠ²Π° Π½Π° ΡΡΠ°Π΄ΠΈΠΈ Π²ΡΡΡΡΠΈΠ²Π°Π½ΠΈΡ ΠΏΠ»Π΅Π½ΠΊΠΈ ΠΈ Π½Π°Π±Π»ΡΠ΄Π°Π΅ΡΡΡ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΠΌΠΎΠ΄ΡΠ»Π΅ΠΉ Π·Π° ΡΡΠ΅Ρ ΠΏΠ°ΡΠ°Π»Π»Π΅Π»ΡΠ½ΠΎΠΉ ΠΎΡΠΈΠ΅Π½ΡΠ°ΡΠΈΠΈ ΡΠ°ΡΡΠΈΡ ΡΠ»ΠΎΠΈΡΡΡΡ
ΡΠΈΠ»ΠΈΠΊΠ°ΡΠΎΠ²
ΠΠ½ΡΠ΅Π½ΡΠΈΠ²Π½ΠΎΡΡΡ Ρ Π΅ΠΌΠΈΠ»ΡΠΌΠΈΠ½Π΅ΡΡΠ΅Π½ΡΠΈΠΈ, ΡΠΎΡΡΠΎΡΠ½ΠΈΠ΅ Π°Π½ΡΠΈΠΎΠΊΡΠΈΠ΄Π°Π½ΡΠ½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ ΠΈ ΠΎΠΊΠΈΡΠ»ΠΈΡΠ΅Π»ΡΠ½Π°Ρ ΠΌΠΎΠ΄ΠΈΡΠΈΠΊΠ°ΡΠΈΡ Π±Π΅Π»ΠΊΠΎΠ² ΠΏΠ»Π°Π·ΠΌΡ ΠΊΡΠΎΠ²ΠΈ ΠΏΡΠΈ ΡΠ°Π·Π²ΠΈΡΠΈΠΈ ΡΠ΅ΡΠΈΠ΄ΠΈΠ²Π° ΡΠ°ΠΊΠ° ΡΠΈΡΠ½ΠΈΠΊΠΎΠ²
The state of antioxidant system (the activity of catalase and ceruloplasmine), the intensity of chemiluminescence, and oxidative modification of proteins were studied in blood plasma of 61 patients with ovarian cancer (cystadenocarcinoma) IIIβIV stage in state of remission and ones with a local recurrent tumor. The dynamics of these indices was analyzed in dependence of recurrent tumor vascularization. The statistically significant changes of the activity of some links of antioxidant system were found, as well as chemiluminescence intensity increase in blood plasma. The level of oxidative modificated protein molecules was raised, the most expressed for products of the main character (530 nm). The dynamics of level of carbonyl derivatives of the neutral and main character (370 and 530 nm) was opposite directed during intensification of recurrent tumour vascularization and increase in speed of blood-groove in a recurrent tumorΠ£ 61 Π±ΠΎΠ»ΡΠ½ΠΎΠΉ ΡΠ°ΠΊΠΎΠΌ ΡΠΈΡΠ½ΠΈΠΊΠΎΠ² (ΡΠΈΡΡΠ°Π΄Π΅Π½ΠΎΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΠ°) IIIβIV ΡΡΠ°Π΄ΠΈΠΉ Π² ΡΠΎΡΡΠΎΡΠ½ΠΈΠΈ ΡΠ΅ΠΌΠΈΡΡΠΈΠΈ ΠΈ Ρ Π»ΠΎΠΊΠ°Π»ΡΠ½ΡΠΌ ΡΠ΅ΡΠΈΠ΄ΠΈΠ²ΠΎΠΌ ΠΈΡ- ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Ρ Π² ΠΏΠ»Π°Π·ΠΌΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΈΠ½ΡΠ΅Π½ΡΠΈΠ²Π½ΠΎΡΡΡ Ρ
Π΅ΠΌΠΈΠ»ΡΠΌΠΈΠ½Π΅ΡΡΠ΅Π½ΡΠΈΠΈ, ΡΠΎΡΡΠΎΡΠ½ΠΈΠ΅ ΡΡΠ΄Π° Π·Π²Π΅Π½ΡΠ΅Π² Π°Π½ΡΠΈΠΎΠΊΡΠΈΠ΄Π°Π½ΡΠ½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ (Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΊΠ°ΡΠ°Π»Π°Π·Ρ, ΡΠ΅ΡΡΠ»ΠΎΠΏΠ»Π°Π·ΠΌΠΈΠ½Π°) ΠΈ ΡΡΠΎΠ²Π΅Π½Ρ ΠΎΠΊΠΈΡΠ»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ ΠΌΠΎΠ΄ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ Π±Π΅Π»ΠΊΠΎΠ² ΠΎΠ±ΡΠ΅ΠΏΡΠΈΠ½ΡΡΡΠΌΠΈ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΎΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ. ΠΡΠΎΠ°Π½Π°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π° Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠ° ΠΈΠ·ΡΡΠ΅Π½Π½ΡΡ
ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ Π² ΠΊΡΠΎΠ²ΠΈ Π±ΠΎΠ»ΡΠ½ΡΡ
Ρ ΡΠ΅ΡΠΈΠ΄ΠΈΠ²ΠΎΠΌ Π² Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ ΠΎΡ ΠΈΠ½ΡΠ΅Π½ΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΊΡΠΎΠ²ΠΎΡΠΎΠΊΠ° Π² ΡΠ΅ΡΠΈΠ΄ΠΈΠ²Π½ΠΎΠΉ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ. ΠΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΎ Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΠΎΠ΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π½Π΅ΠΊΠΎΡΠΎΡΡΡ
Π·Π²Π΅Π½ΡΠ΅Π² Π°Π½ΡΠΈΠΎΠΊΡΠΈΠ΄Π°Π½ΡΠ½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ, ΡΡΠΈΠ»Π΅Π½ΠΈΠ΅ Ρ
Π΅ΠΌΠΈΠ»ΡΠΌΠΈΠ½Π΅ΡΡΠ΅Π½ΡΠΈΠΈ ΠΏΠ»Π°Π·ΠΌΡ ΠΊΡΠΎΠ²ΠΈ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ ΠΎΠΊΠΈΡΠ»Π΅Π½Π½ΠΎΡΡΠΈ Π±Π΅Π»ΠΊΠΎΠ²ΡΡ
ΠΌΠΎΠ»Π΅ΠΊΡΠ», Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΠ΅ Π΄Π»Ρ ΠΏΡΠΎΠ΄ΡΠΊΡΠΎΠ² ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠ³ΠΎ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠ° (530 Π½ΠΌ). ΠΠΎ ΠΌΠ΅ΡΠ΅ ΠΏΡΠΎΠ³ΡΠ΅ΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΎΠ½ΠΊΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ° ΠΏΡΠΈ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ ΡΠ΅ΡΠΈΠ΄ΠΈΠ²Π½ΠΎΠΉ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ, ΡΡΠΈΠ»Π΅Π½ΠΈΠΈ Π΅Π΅ Π²Π°ΡΠΊΡΠ»ΡΡΠΈΠ·Π°ΡΠΈΠΈ ΠΈ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠΈ ΡΠΊΠΎΡΠΎΡΡΠΈ ΠΊΡΠΎΠ²ΠΎΡΠΎΠΊΠ° Π½Π°Π±Π»ΡΠ΄Π°Π΅ΡΡΡ ΠΏΡΠΎΡΠΈΠ²ΠΎΠΏΠΎΠ»ΠΎΠΆΠ½ΠΎ Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½Π°Ρ Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠ° ΡΡΠΎΠ²Π½Ρ ΠΊΠ°ΡΠ±ΠΎΠ½ΠΈΠ»ΡΠ½ΡΡ
ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΡ
Π½Π΅ΠΉΡΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΈ ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠ³ΠΎ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠ° (370 ΠΈ 530 Π½ΠΌ
EFFECT OF UROKINASE GENE-KNOCKOUT ON GROWTH OF MELANOMA IN EXPERIMENT
The purpose of the study was to reveal special features of the Π16/F10 melanoma growth in urokinase (uPA) geneΒ knockout mice with and without chronic neurogenic pain (CNP). Material and methods. The study included male andΒ female Π‘57ΠL/6 mice (n = 102) and C57BL/6-Plautm1.1BugThisPlauGFDhu/GFDhu mice with uPA gene knockoutΒ (n = 48). Mice of the main subgroups underwent subcutaneous transplantation of Π16/F10 melanoma 2 weeks afterΒ bilateral ligation of sciatic nerves (CNP model); mice of the same strain with standard melanoma transplantation servedΒ as controls. Results and discussion. Survival of uPA gene knockout mice did not differ from that of normal animals βΒ 1.5 times higher in females than in males (p < 0.05), with melanoma onset in gene-deficient mice a week earlier. TheΒ dynamics of tumor growth had pronounced gender differences: in females, the tumor did not grow and its maximalΒ volume prior to death was 1.0 cm3, while tumors in males were characterized by an active growth with two peaks ofΒ volume increase (weeks 2 and 4). Melanoma was weakly metastatic β solitary metastases to the lungs (in females) orΒ no metastases, but pulmonary and heart hemorrhages were noted (in males). CNP decreased the survival of uPA geneΒ knockout females, as well as of normal animals, but did not influence the survival of males; primary tumors in genedeficientΒ mice appeared a few days later than in controls but their growth was more intense, with diminished genderΒ differences. Increased metastasis was manifested by the initiation of metastatic lesions to the lungs and liver in males,Β with maintained pulmonary hemorrhages, and by increased number of metastatic foci in the lungs together with theΒ appearance of pulmonary hemorrhages in females. Conclusions. The influence of uPA gene knockout on the courseΒ of Π16/F10 melanoma differs in male and female mice. CNP enhances malignant tumor growth, diminishing genderΒ differences, and activates melanoma metastasis
Π Π΅Π΄ΠΎΠΊΡ ΡΠΎΡΠΌΡ Π³Π»ΡΡΠ°ΡΠΈΠΎΠ½Π° ΠΏΡΠΈ Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠΌ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΠΈ ΠΆΠ΅Π»ΡΠ΄ΠΊΠ° ΡΠ°Π·Π½ΠΎΠΉ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ Π°Π³ΡΠ΅ΡΡΠΈΠ²Π½ΠΎΡΡΠΈ
Aim. To study the levels of reduced and oxidized glutathione (GSH and GSSG, respectively), as well as the thiolΒ status in gastric cancer (GC) tumors of various histological types and grades.Materials and methods. The indicators were determined by ELISA methods in tumor, peritumoral and visuallyΒ intact tissues obtained during surgery from 52 patients with GC: 18 patients had a G1-2 adenocarcinoma (AC), 8Β with G3 AC, 6 with signet ring cell cancer (SRCC), 14 with combined gastric lesions (CGL) β AC with signet ringΒ cell fragments, 6 with patients with a component of undifferentiated cancer, G4.Results. In the groups of patients with low-differentiated and undifferentiated tumors, the GSH content in the tumorΒ tissue and the peritumoral zone was higher than in the group of patients with well- and moderately-differentiatedΒ tumors. Tumor GSH levels in G3 AC and SRCC exceeded the values in visually intact tissues. Moreover, in theΒ visually intact tissue of patients with SRCC, GSH level was reduced relative to G1-2 AC and CGL. GSH in allΒ tissues of patients with CGL was higher than in patients with G1-2 AC. The lowest level of GSSG in the tumorΒ tissue was registered in SRCC: 27.5% lower than in G1-2 AC and 30.3% lower than in G3 AC. Patients withΒ undifferentiated tumors (G4 AC) had the highest GSH content in all studied tissues: by 29.9% in tumor; by 40.7%Β in peritumoral zone; and in visually intact tissue not only GSH, but also GSSG was increased by 22.5β25.5% inΒ comparison with AC G1-2. G4 AC was also characterized by a sharp increase in the thiol status in tumor tissuesΒ by 80.2 and 89.9% higher than in visually intact and peritumoral tissues, and it was statistically higher than in ACΒ G1-2, AC G3, SRCC and CGL. The ratio of GSH and GSSG was the most informative.Conclusion. Poor AC differentiation (in the row G1-2, G3, G4) and a change of histological tumor type (AC, SPLΒ and SRCC), i.e. an increase in tumor aggressiveness, were accompanied by the enhancement of reductive processesΒ in tumor tissue, as evidenced by the statistically significant increase in the GSH/GSSG coefficient and a sharpΒ increase in the thiol status in G4 AC.Π¦Π΅Π»Ρ. ΠΠ·ΡΡΠΈΡΡ ΡΡΠΎΠ²Π΅Π½Ρ Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π½ΠΎΠ³ΠΎ ΠΈ ΠΎΠΊΠΈΡΠ»Π΅Π½Π½ΠΎΠ³ΠΎ Π³Π»ΡΡΠ°ΡΠΈΠΎΠ½Π° (GSH ΠΈ GSSG ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ), Π° ΡΠ°ΠΊΠΆΠ΅ ΡΠΈΠΎΠ»ΠΎΠ²ΡΠΉ ΡΡΠ°ΡΡΡ Π² ΠΎΠΏΡΡ
ΠΎΠ»ΡΡ
ΡΠ°ΠΊΠ° ΠΆΠ΅Π»ΡΠ΄ΠΊΠ° (Π Π) ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
Π³ΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠΈΠΏΠΎΠ² ΠΈ ΡΠ°Π·Π½ΠΎΠΉ ΡΡΠ΅ΠΏΠ΅Π½ΠΈΒ Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΡΠΎΠ²ΠΊΠΈ.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Ρ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π² ΠΎΠ±ΡΠ°Π·ΡΠ°Ρ
Β ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ, ΠΏΠ΅ΡΠΈΡΡΠΌΠΎΡΠ°Π»ΡΠ½ΠΎΠΉ Π·ΠΎΠ½Ρ ΠΈ Π²ΠΈΠ·ΡΠ°Π»ΡΠ½ΠΎ ΠΈΠ½ΡΠ°ΠΊΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ. ΠΠ±ΡΠ°Π·ΡΡ ΠΏΠΎΠ»ΡΡΠ΅Π½Ρ Π²ΠΎ Π²ΡΠ΅ΠΌΡ ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΈΒ Ρ 52 Π±ΠΎΠ»ΡΠ½ΡΡ
Π Π, Π² ΡΠΎΠΌ ΡΠΈΡΠ»Π΅ Ρ 18 β Ρ Π°Π΄Π΅Π½ΠΎΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΠΎΠΉ (ΠΠ) G1-2, 8 β Ρ ΠΠ G3, 6 β Ρ ΠΏΠ΅ΡΡΡΠ½Π΅Π²ΠΈΠ΄Π½ΠΎΠΊΠ»Π΅ΡΠΎΡΠ½ΡΠΌΒ ΡΠ°ΠΊΠΎΠΌ (ΠΠΠ ), 14 β Ρ ΡΠΎΡΠ΅ΡΠ°Π½Π½ΡΠΌ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΠ΅ΠΌ ΠΆΠ΅Π»ΡΠ΄ΠΊΠ° (Π‘ΠΠ) ΠΈ 6 β Ρ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠΎΠΌ Π½Π΅Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎΒ ΡΠ°ΠΊΠ° G4.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π Π³ΡΡΠΏΠΏΠ°Ρ
Π±ΠΎΠ»ΡΠ½ΡΡ
Ρ Π½ΠΈΠ·ΠΊΠΎΠ΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠΌΠΈ ΠΈ Π½Π΅Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠΌΠΈ ΠΎΠΏΡΡ
ΠΎΠ»ΡΠΌΠΈΒ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ GSH Π² ΡΠΊΠ°Π½ΠΈ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΈ ΠΏΠ΅ΡΠΈΡΠΎΠΊΠ°Π»ΡΠ½ΠΎΠΉ Π·ΠΎΠ½Ρ Π±ΡΠ»ΠΎ Π²ΡΡΠ΅, ΡΠ΅ΠΌ Π² Π³ΡΡΠΏΠΏΠ΅ Π±ΠΎΠ»ΡΠ½ΡΡ
Ρ Π²ΡΡΠΎΠΊΠΎ- ΠΈΒ ΡΠΌΠ΅ΡΠ΅Π½Π½ΠΎ Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠΌΠΈ ΠΎΠΏΡΡ
ΠΎΠ»ΡΠΌΠΈ. ΠΡΠΈ ΠΠ G3 ΠΈ ΠΠΠ ΡΡΠΎΠ²Π΅Π½Ρ GSH Π² ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ Π·Π½Π°ΡΠΈΠΌΠΎΒ ΠΏΡΠ΅Π²ΡΡΠ°Π» ΡΡΠΎΠ²Π΅Π½Ρ Π² Π²ΠΈΠ·ΡΠ°Π»ΡΠ½ΠΎ ΠΈΠ½ΡΠ°ΠΊΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ. ΠΡΠΈ ΡΡΠΎΠΌ Π² Π²ΠΈΠ·ΡΠ°Π»ΡΠ½ΠΎ ΠΈΠ½ΡΠ°ΠΊΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ Π±ΠΎΠ»ΡΠ½ΡΡ
ΠΠΠ Β ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ GSH Π±ΡΠ»ΠΎ Π½ΠΈΠΆΠ΅, ΡΠ΅ΠΌ ΠΏΡΠΈ ΠΠ G1-2 ΠΈ Π‘ΠΠ. ΠΡΠΈ Π‘ΠΠ ΡΡΠΎΠ²Π΅Π½Ρ GSH Π²ΠΎ Π²ΡΠ΅Ρ
ΡΠΊΠ°Π½ΡΡ
Π±ΡΠ» Π²ΡΡΠ΅,Β ΡΠ΅ΠΌ ΠΏΡΠΈ ΠΠ G1-2.ΠΠ°ΠΈΠ±ΠΎΠ»Π΅Π΅ Π½ΠΈΠ·ΠΊΠΈΠΉ ΡΡΠΎΠ²Π΅Π½Ρ GSSG Π² ΡΠΊΠ°Π½ΠΈ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΎΡΠΌΠ΅ΡΠ΅Π½ ΠΏΡΠΈ ΠΠΠ : Π½Π° 27,5% Π½ΠΈΠΆΠ΅, ΡΠ΅ΠΌ ΠΏΡΠΈ ΠΠ G1-2, ΠΈ Π½Π° 30,3% ΠΎΡΠ½ΠΎΡΠΈΡΠ΅Π»ΡΠ½ΠΎ ΠΠ G3. ΠΡΠΈ ΠΠ G4 Π½Π°Π±Π»ΡΠ΄Π°Π»ΠΎΡΡ ΡΠ°ΠΌΠΎΠ΅ Π²ΡΡΠΎΠΊΠΎΠ΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ GSH Π²ΠΎ Π²ΡΠ΅Ρ
Β ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Π½ΡΡ
ΡΠΊΠ°Π½ΡΡ
: Π² ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ β Π½Π° 29,9%, Π² ΠΏΠ΅ΡΠΈΡΠΎΠΊΠ°Π»ΡΠ½ΠΎΠΉ Π·ΠΎΠ½Π΅ β Π½Π° 40,7%, Π° Π² Π²ΠΈΠ·ΡΠ°Π»ΡΠ½ΠΎ ΠΈΠ½ΡΠ°ΠΊΡΠ½ΠΎΠΉΒ ΡΠΊΠ°Π½ΠΈ Π½Π΅ ΡΠΎΠ»ΡΠΊΠΎ GSH, Π½ΠΎ ΠΈ GSSG Π½Π° 22,5β25,5% ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ ΡΠΎ Π·Π½Π°ΡΠ΅Π½ΠΈΡΠΌΠΈ Ρ Π±ΠΎΠ»ΡΠ½ΡΡ
ΠΠ G1-2. ΠΠ»Ρ G4Β ΡΠ°ΠΊΠΆΠ΅ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠ΅Π½ Π²ΡΡΠΎΠΊΠΈΠΉ ΡΡΠΎΠ²Π΅Π½Ρ ΡΠΈΠΎΠ»ΠΎΠ²ΠΎΠ³ΠΎ ΡΡΠ°ΡΡΡΠ° Π² ΡΠΊΠ°Π½ΠΈ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ β Π½Π° 80,2 ΠΈ 89,9%Β Π²ΡΡΠ΅, ΡΠ΅ΠΌ Π² Π²ΠΈΠ·ΡΠ°Π»ΡΠ½ΠΎ ΠΈΠ½ΡΠ°ΠΊΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ ΠΈ ΠΏΠ΅ΡΠΈΡΡΠΌΠΎΡΠ°Π»ΡΠ½ΠΎΠΉ Π·ΠΎΠ½Π΅, ΠΈ ΠΎΠ½ Π±ΡΠ» Π·Π½Π°ΡΠΈΠΌΠΎΒ Π²ΡΡΠ΅ (Π½Π° 68β96%), ΡΠ΅ΠΌ ΠΏΡΠΈ ΠΠ G1-2,Β ΠΠ G3, ΠΠΠ ΠΈ Π‘ΠΠ. ΠΠ°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠ²Π½ΡΠΌ ΠΎΠΊΠ°Π·Π°Π»ΠΎΡΡ ΡΠΎΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠ΅ Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π½ΠΎΠΉ ΠΈ ΠΎΠΊΠΈΡΠ»Π΅Π½Π½ΠΎΠΉΒ ΡΠΎΡΠΌ Π³Π»ΡΡΠ°ΡΠΈΠΎΠ½Π°.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΡΠΈ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠΈ Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΡΠΎΠ²ΠΊΠΈ ΠΠ (Π² ΡΡΠ΄Ρ G1-2, G3, G4) ΠΈ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠΈ Π³ΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎΒ ΡΠΈΠΏΠ° ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ (ΠΠ, Π‘ΠΠ ΠΈ ΠΠΠ ), Ρ.Π΅. ΠΏΡΠΈ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠΈ Π°Π³ΡΠ΅ΡΡΠΈΠ²Π½ΠΎΡΡΠΈ Π½Π΅ΠΎΠΏΠ»Π°Π·ΠΌΡ, ΠΏΡΠΎΠΈΡΡ
ΠΎΠ΄ΠΈΡ ΡΡΠΈΠ»Π΅Π½ΠΈΠ΅Β Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²ΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ² Π² ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ, ΠΎ ΡΠ΅ΠΌ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΠ΅Ρ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π½Π°ΡΠΈΠΌΠΎ Π±ΠΎΠ»Π΅Π΅Β Π²ΡΡΠΎΠΊΠΈΠΉ ΠΊΠΎΡΡΡΠΈΡΠΈΠ΅Π½Ρ GSH/GSSG ΠΈ ΡΡΠΎΠ²Π΅Π½Ρ ΡΠΈΠΎΠ»ΠΎΠ²ΠΎΠ³ΠΎ ΡΡΠ°ΡΡΡΠ° Π² ΡΠ»ΡΡΠ°Π΅ ΠΠ G4
ΠΠΠΠ―ΠΠΠ Π₯Π ΠΠΠΠ§ΠΠ‘ΠΠΠ ΠΠΠΠ ΠΠΠΠΠΠΠ ΠΠΠΠ ΠΠ ΠΠΠΠΠΠ¬ΠΠΠ Π‘ΠΠΠΠ ΠΠΠΠΠ ΠΠΠΠΠΠ«Π₯ ΠΠΠ ΠΠΠΠΠ Π Π ΠΠ¦ΠΠΠ’ΠΠ ΠΠ Π ΠΠΠΠ, ΠΠΠ£Π₯ΠΠΠ Π ΠΠΠ ΠΠ€ΠΠΠΠΠ¬ΠΠΠ ΠΠΠΠ Π£ Π‘ΠΠΠ¦ΠΠ Π‘ ΠΠΠ ΠΠΠΠΠΠΠ ΠΠΠΠΠΠΠΠΠ Π16/F10
Local synthesis and metabolism of sex hormones allow the skin to regulate autonomously both nociceptive reactions and proliferative processes in various pathologies. The purpose of the study was to reveal theΒ influence of chronic neurogenic pain on the balance of sex hormones in the skin, tumor and perifocal area inΒ male mice in the dynamics of Π16/F10 melanoma growth. Material and methods. The study included Π‘57ΠL/6Β male mice. Animals were divided into the main group β animals with Π16/F10 melanoma transplanted afterΒ the development of chronic neurogenic pain (n=25), comparison group (n=27) β animals with standard tumorΒ transplantation, controls β males with chronic neurogenic pain (n=8), and intact mice (n=8). Levels of estradiolΒ (E2), estrone (E1), testosterone (T), progesterone (P4) (CUSABIO, China), estrogen receptors β ERΞ±, ERΞ²Β (Π‘loud-Clone Corp. USA, China), progesterone (RP4) and androgens (RA) (CUSABIO, China) were determined in 10 % homogenates of skin, tumor and perifocal tissues 1, 2 and 3 weeks after Π16/F10 melanomaΒ transplantation using standard ELISA test systems adapted for experimental animals. Results. Chronic neurogenic pain causes an imbalance of sex steroids with marked hyperestrogenism. The imbalance is achievedΒ by increased content of estradiol and receptors of estrogen, increased concentration of progesterone withΒ inhibition of its receptor apparatus, as well as reduced levels of testosterone with increased concentration ofΒ androgen receptors. Melanoma in animals with chronic neurogenic pain grows and develops in a target organΒ with an altered steroid balance, which leads to changes in the tumor characteristics. Chronic neurogenic painΒ and B16/F10 melanoma has a unidirectional effect on the local hormonal status of the skin.ΠΠΎΠΊΠ°Π»ΡΠ½ΡΠΉ ΡΠΈΠ½ΡΠ΅Π· ΠΈ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌ ΠΏΠΎΠ»ΠΎΠ²ΡΡ
Π³ΠΎΡΠΌΠΎΠ½ΠΎΠ² ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡ ΠΊΠΎΠΆΠ΅ ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΡΡ Π°Π²ΡΠΎΠ½ΠΎΠΌΠ½ΡΡ ΡΠ΅Π³ΡΠ»ΡΡΠΈΡ ΠΊΠ°ΠΊ Π½ΠΎΡΠΈΡΠ΅ΠΏΡΠΈΠ²Π½ΡΡ
ΡΠ΅Π°ΠΊΡΠΈΠΉ, ΡΠ°ΠΊ ΠΈ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΡΡ
ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ² ΠΏΡΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
Β ΡΠΎΡΡΠΎΡΠ½ΠΈΡΡ
. Π¦Π΅Π»ΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΠ²ΠΈΠ»ΠΎΡΡ ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ Π²Π»ΠΈΡΠ½ΠΈΡ Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π½Π΅ΠΉΡΠΎΠ³Π΅Π½Π½ΠΎΠΉ Π±ΠΎΠ»ΠΈ Π½Π°Β Π±Π°Π»Π°Π½Ρ ΠΏΠΎΠ»ΠΎΠ²ΡΡ
Π³ΠΎΡΠΌΠΎΠ½ΠΎΠ² ΠΈ ΠΈΡ
ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΠΎΠ² Π² ΠΊΠΎΠΆΠ΅, ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΈ ΠΏΠ΅ΡΠΈΡΠΎΠΊΠ°Π»ΡΠ½ΠΎΠΉ Π·ΠΎΠ½Π΅ Ρ ΡΠ°ΠΌΡΠΎΠ² ΠΌΡΡΠ΅ΠΉΒ Π² Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠ΅ ΡΠΎΡΡΠ° ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ Π16/F10. ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. Π ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ΅ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈΡΡ ΡΠ°ΠΌΡΡΒ ΠΌΡΡΠ΅ΠΉ Π»ΠΈΠ½ΠΈΠΈ Π‘57ΠL/6. ΠΡΠ½ΠΎΠ²Π½ΡΡ Π³ΡΡΠΏΠΏΡ ΡΠΎΡΡΠ°Π²ΠΈΠ»ΠΈ ΠΆΠΈΠ²ΠΎΡΠ½ΡΠ΅, Ρ ΠΊΠΎΡΠΎΡΡΡ
ΠΌΠ΅Π»Π°Π½ΠΎΠΌΠ° Π16/F10 ΠΏΠ΅ΡΠ΅Π²ΠΈΠ²Π°Π»Π°ΡΡ ΠΏΠΎΡΠ»Π΅ ΡΠ°Π·Π²ΠΈΡΠΈΡ Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π½Π΅ΠΉΡΠΎΠ³Π΅Π½Π½ΠΎΠΉ Π±ΠΎΠ»ΠΈ (n=25), Π³ΡΡΠΏΠΏΡ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ (n=27) β ΠΆΠΈΠ²ΠΎΡΠ½ΡΠ΅ ΡΠΎΒ ΡΡΠ°Π½Π΄Π°ΡΡΠ½ΠΎΠΉ ΠΏΠ΅ΡΠ΅Π²ΠΈΠ²ΠΊΠΎΠΉ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ, ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ β ΡΠ°ΠΌΡΡ Ρ Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π½Π΅ΠΉΡΠΎΠ³Π΅Π½Π½ΠΎΠΉ Π±ΠΎΠ»ΡΡ (n=8), Π° ΡΠ°ΠΊΠΆΠ΅Β ΠΈΠ½ΡΠ°ΠΊΡΠ½ΡΠ΅ ΠΌΡΡΠΈ (n=8). Π§Π΅ΡΠ΅Π· 1, 2 ΠΈ 3 Π½Π΅Π΄ ΠΏΠΎΡΠ»Π΅ ΠΏΠ΅ΡΠ΅Π²ΠΈΠ²ΠΊΠΈ ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ Π16/F10 Π² 10 % Π³ΠΎΠΌΠΎΠ³Π΅Π½Π°ΡΠ°Ρ
Β ΠΊΠΎΠΆΠΈ, ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΈ ΠΏΠ΅ΡΠΈΡΠΎΠΊΠ°Π»ΡΠ½ΠΎΠΉ Π·ΠΎΠ½Π΅ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΡΡΠ°Π½Π΄Π°ΡΡΠ½ΡΡ
ΡΠ΅ΡΡ-ΡΠΈΡΡΠ΅ΠΌ ΠΠ€Π, Π°Π΄Π°ΠΏΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Π΄Π»ΡΒ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΡ
ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
, ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ ΡΡΠΎΠ²Π΅Π½Ρ ΡΡΡΡΠ°Π΄ΠΈΠΎΠ»Π° (Π2), ΡΡΡΡΠΎΠ½Π° (Π1), ΡΠ΅ΡΡΠΎΡΡΠ΅ΡΠΎΠ½Π° (Π’),Β ΠΏΡΠΎΠ³Π΅ΡΡΠ΅ΡΠΎΠ½Π° (Π 4) (CUSABIO, ΠΠΈΡΠ°ΠΉ), ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΠΎΠ² ΡΡΡΡΠΎΠ³Π΅Π½ΠΎΠ² β ERΞ±, ERΞ² (Π‘loud-Clone Corp. Π‘Π¨Π, ΠΠΈΡΠ°ΠΉ), ΠΏΡΠΎΠ³Π΅ΡΡΠ΅ΡΠΎΠ½Π° (RP4) ΠΈ Π°Π½Π΄ΡΠΎΠ³Π΅Π½ΠΎΠ² (RA) (CUSABIO, ΠΠΈΡΠ°ΠΉ). Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π₯ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠ°Ρ Π½Π΅ΠΉΡΠΎΠ³Π΅Π½Π½Π°ΡΒ Π±ΠΎΠ»Ρ Π²ΡΠ·ΡΠ²Π°Π΅Ρ Π΄ΠΈΡΠ±Π°Π»Π°Π½Ρ ΠΏΠΎΠ»ΠΎΠ²ΡΡ
ΡΡΠ΅ΡΠΎΠΈΠ΄ΠΎΠ² Ρ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΠΉ Π³ΠΈΠΏΠ΅ΡΡΡΡΡΠΎΠ³Π΅Π½ΠΈΠ΅ΠΉ. ΠΠ°ΡΡΡΠ΅Π½ΠΈΠ΅ ΡΠ°Π²Π½ΠΎΠ²Π΅ΡΠΈΡΒ ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΠ΅ΡΡΡ Π·Π° ΡΡΠ΅Ρ ΡΠΎΡΡΠ° ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΡΡΡΡΠ°Π΄ΠΈΠΎΠ»Π° ΠΈ ΡΡΡΡΠΎΠ³Π΅Π½ΠΎΠ²ΡΡ
ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΠΎΠ², ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΠΏΡΠΎΠ³Π΅ΡΡΠ΅ΡΠΎΠ½Π° Ρ ΡΠ³Π½Π΅ΡΠ΅Π½ΠΈΠ΅ΠΌ Π΅Π³ΠΎ ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΠ½ΠΎΠ³ΠΎ Π°ΠΏΠΏΠ°ΡΠ°ΡΠ°, Π° ΡΠ°ΠΊΠΆΠ΅ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ΠΌ ΡΡΠΎΠ²Π½Ρ ΡΠ΅ΡΡΠΎΡΡΠ΅ΡΠΎΠ½Π°Β Π½Π° ΡΠΎΠ½Π΅ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΠΎΠ² Π°Π½Π΄ΡΠΎΠ³Π΅Π½ΠΎΠ². Π ΠΎΡΡ ΠΈ ΡΠ°Π·Π²ΠΈΡΠΈΠ΅ ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ Ρ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
ΡΒ Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π½Π΅ΠΉΡΠΎΠ³Π΅Π½Π½ΠΎΠΉ Π±ΠΎΠ»ΡΡ ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΠ΅ΡΡΡ Π² ΠΎΡΠ³Π°Π½-ΠΌΠΈΡΠ΅Π½Ρ Ρ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½Π½ΡΠΌ ΡΡΠ΅ΡΠΎΠΈΠ΄Π½ΡΠΌ Π±Π°Π»Π°Π½ΡΠΎΠΌ, ΡΡΠΎ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡΠΌ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠΈ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ. Π₯ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠ°Ρ Π½Π΅ΠΉΡΠΎΠ³Π΅Π½Π½Π°Ρ Π±ΠΎΠ»Ρ ΠΎΠΊΠ°Π·ΡΠ²Π°Π΅ΡΒ ΠΎΠ΄Π½ΠΎΠ½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΠΎΠ΅ Ρ ΠΌΠ΅Π»Π°Π½ΠΎΠΌΠΎΠΉ Π16/F10 Π²Π»ΠΈΡΠ½ΠΈΠ΅ Π½Π° Π»ΠΎΠΊΠ°Π»ΡΠ½ΡΠΉ Π³ΠΎΡΠΌΠΎΠ½Π°Π»ΡΠ½ΡΠΉ ΡΡΠ°ΡΡΡ ΠΊΠΎΠΆΠΈ.Β
ΠΠ»ΠΈΡΠ½ΠΈΠ΅ Π²Π°ΡΠΈΠ°Π½ΡΠ° ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ B16/F10 Π½Π° ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ ΡΠΈΡΠΎΡ ΡΠΎΠΌΠ° Π‘ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΡΡ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ ΠΎΡΠ³Π°Π½ΠΎΠ² ΡΠ°ΠΌΠΎΠΊ ΠΌΡΡΠ΅ΠΉ
Introduction. Cytochrome C in mitochondria transfers electrons from complex III to complex IV, and it is a signaling molecule in the apoptosis realization.The objective was to evaluate the level of cytochrome C in cell mitochondria in various organs of female mice with standard and stimulated growth of experimental B16/F10 melanoma.Methods and materials. The experiment was performed on female C57BL/6 mice (n=168). The groups were: intact animals (n=21); controls with a model of chronic neurogenic pain (CNP) (n = 21); group M β standard B16/F10 melanoma transplantation (n=63), group CNP + M β B16/F10 melanoma transplantation 3 weeks after CNP model creation (n=63). The level of cytochrome C (ng / mg protein) were measured by ELISA (Bioscience, Austria). Statistical analysis of results was performed using the Β«Statistica 10.0Β» program.Results. After 1 week of standard melanoma growth, an increase in the level of cytochrome C by 2.7 and 1.7 times was detected in mitochondria of the brain and liver; by the 3rd week, it decreased in the liver and skin by 1.7 times. In melanoma mitochondria, the level of cytochrome C was lower than in the skin of intact animals: by 2.5 times after week 1, by 4.5 times after week 2, and by 4.6 times after week 3. After 1 week of stimulated melanoma growth, the level of cytochrome C decreased compared control values: by 2.2 times in the brain, by 1.9 times in the liver, by 1.4 times in the skin; by week 3, it decreased by 4.8 times in mitochondria of the brain, by 4.7 times β in the liver, by 2.3 times β in the heart, by 1.9 times β in the skin. In melanoma mitochondria, the level of cytochrome C was lower than in the skin of intact animals: by 15.3 times after week 1, by 10.3 times after week 2, and by 8.8 times after week 3.Conclusion. Low level of cytochrome C were found in melanoma mitochondria in standard and stimulated tumor growth. The data can be used in the experiment and in clinic for using exogenous cytochrome C as an agent slowing down the malignant process.ΠΠ²Π΅Π΄Π΅Π½ΠΈΠ΅. Π¦ΠΈΡΠΎΡ
ΡΠΎΠΌ Π‘ Π² ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΡΡ
ΠΏΠ΅ΡΠ΅Π½ΠΎΡΠΈΡ ΡΠ»Π΅ΠΊΡΡΠΎΠ½Ρ ΠΎΡ III ΠΊ IV ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΡ ΠΈ ΡΠ²Π»ΡΠ΅ΡΡΡ ΡΠΈΠ³Π½Π°Π»ΡΠ½ΠΎΠΉ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΠΎΠΉ Π² ΡΠ΅Π°Π»ΠΈΠ·Π°ΡΠΈΠΈ Π°ΠΏΠΎΠΏΡΠΎΠ·Π°.Π¦Π΅Π»Ρ β ΠΈΠ·ΡΡΠΈΡΡ ΡΡΠΎΠ²Π΅Π½Ρ ΡΠΈΡΠΎΡ
ΡΠΎΠΌΠ° Π‘ Π² ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΠΎΡΠ³Π°Π½ΠΎΠ² ΠΌΡΡΠ΅ΠΉ-ΡΠ°ΠΌΠΎΠΊ ΠΏΡΠΈ ΡΡΠ°Π½Π΄Π°ΡΡΠ½ΠΎΠΌ ΠΈ ΡΡΠΈΠΌΡΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΌ ΡΠΎΡΡΠ΅ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ B16/F10.ΠΠ΅ΡΠΎΠ΄Ρ ΠΈ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ. Π ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ΅ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ ΠΌΡΡΠ΅ΠΉ-ΡΠ°ΠΌΠΎΠΊ Π»ΠΈΠ½ΠΈΠΈ C57BL/6 (n=168). ΠΡΡΠΏΠΏΡ: ΠΈΠ½ΡΠ°ΠΊΡΠ½Π°Ρ (n = 21); ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½Π°Ρ β ΠΌΠΎΠ΄Π΅Π»Ρ Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π½Π΅ΠΉΡΠΎΠ³Π΅Π½Π½ΠΎΠΉ Π±ΠΎΠ»ΠΈ (Π₯ΠΠ) (n=21); Π³ΡΡΠΏΠΏΠ° Π β ΡΡΠ°Π½Π΄Π°ΡΡΠ½Π°Ρ ΡΡΠ°Π½ΡΠΏΠ»Π°Π½ΡΠ°ΡΠΈΡ ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ B16/F10 (n=63); Π³ΡΡΠΏΠΏΠ° Π₯ΠΠ + Π β ΡΡΠ°Π½ΡΠΏΠ»Π°Π½ΡΠ°ΡΠΈΡ ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ B16/F10 ΡΠ΅ΡΠ΅Π· 3 Π½Π΅Π΄Π΅Π»ΠΈ ΠΏΠΎΡΠ»Π΅ ΡΠΎΠ·Π΄Π°Π½ΠΈΡ ΠΌΠΎΠ΄Π΅Π»ΠΈ Π₯ΠΠ (n=63). ΠΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ ΡΡΠΎΠ²Π΅Π½Ρ ΡΠΈΡΠΎΡ
ΡΠΎΠΌΠ° Π‘ (Π½Π³/ΠΌΠ³ Π±Π΅Π»ΠΊΠ°) (Bioscience, Austria). Π‘ΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°Π½Π°Π»ΠΈΠ· ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ² Π²ΡΠΏΠΎΠ»Π½Π΅Π½ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΡ Β«Statistica 10.0Β».Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π§Π΅ΡΠ΅Π· 1 Π½Π΅Π΄Π΅Π»Ρ ΡΡΠ°Π½Π΄Π°ΡΡΠ½ΠΎΠ³ΠΎ ΡΠΎΡΡΠ° ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ Π²ΡΡΠ²ΠΈΠ»ΠΈ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ ΡΡΠΎΠ²Π½Ρ ΡΠΈΡΠΎΡ
ΡΠΎΠΌΠ° Π‘ Π² ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΡΡ
ΠΌΠΎΠ·Π³Π° ΠΈ ΠΏΠ΅ΡΠ΅Π½ΠΈ Π² 2,7 ΠΈ 1,7 ΡΠ°Π·Π°, ΠΊ 3-ΠΉ Π½Π΅Π΄Π΅Π»Π΅ ΡΠΎΡΡΠ° β ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ Π² ΠΏΠ΅ΡΠ΅Π½ΠΈ ΠΈ ΠΊΠΎΠΆΠΈ Π² 1,7 ΡΠ°Π·Π°. Π ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΡΡ
ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ ΡΡΠΎΠ²Π΅Π½Ρ ΡΠΈΡΠΎΡ
ΡΠΎΠΌΠ° Π‘ Π±ΡΠ» Π½ΠΈΠΆΠ΅ ΠΈΠ½ΡΠ°ΠΊΠ½ΡΡ
Π²Π΅Π»ΠΈΡΠΈΠ½ ΠΊΠΎΠΆΠΈ: ΡΠ΅ΡΠ΅Π· 1 Π½Π΅Π΄Π΅Π»Ρ β Π² 2,5 ΡΠ°Π·Π°, 2 Π½Π΅Π΄Π΅Π»ΠΈ β Π² 4,5 ΡΠ°Π·Π°, 3 Π½Π΅Π΄Π΅Π»ΠΈ β Π² 4,6 ΡΠ°Π·Π°. Π§Π΅ΡΠ΅Π· 1 Π½Π΅Π΄Π΅Π»Ρ ΡΡΠΈΠΌΡΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ ΡΠΎΡΡΠ° ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ ΡΡΠΎΠ²Π΅Π½Ρ ΡΠΈΡΠΎΡ
ΡΠΎΠΌΠ° Π‘ ΡΠ½ΠΈΠ·ΠΈΠ»ΡΡ ΠΎΡΠ½ΠΎΡΠΈΡΠ΅Π»ΡΠ½ΠΎ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΡΡ
Π²Π΅Π»ΠΈΡΠΈΠ½: Π² ΠΌΠΎΠ·Π³Π΅ β Π² 2,2 ΡΠ°Π·Π°, ΠΏΠ΅ΡΠ΅Π½ΠΈ β Π² 1,9 ΡΠ°Π·Π°, ΠΊΠΎΠΆΠΈ β Π² 1,4 ΡΠ°Π·Π°, ΠΊ 3-ΠΉ Π½Π΅Π΄Π΅Π»Π΅ Π² ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΡΡ
ΠΌΠΎΠ·Π³Π° β Π² 4,8 ΡΠ°Π·Π°, ΠΏΠ΅ΡΠ΅Π½ΠΈ β Π² 4,7 ΡΠ°Π·Π°, ΡΠ΅ΡΠ΄ΡΠ° β Π² 2,3 ΡΠ°Π·Π°, ΠΊΠΎΠΆΠΈ β Π² 1,9 ΡΠ°Π·Π°. Π ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΡΡ
ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ ΡΠΈΡΠΎΡ
ΡΠΎΠΌΠ° Π‘ Π±ΡΠ»ΠΎ Π½ΠΈΠΆΠ΅ Π·Π½Π°ΡΠ΅Π½ΠΈΠΉ Π² ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ ΠΊΠΎΠΆΠ΅: ΡΠ΅ΡΠ΅Π· 1 Π½Π΅Π΄Π΅Π»Ρ β Π² 15,3 ΡΠ°Π·Π°, 2 Π½Π΅Π΄Π΅Π»ΠΈ β Π² 10,3 ΡΠ°Π·Π°, 3 Π½Π΅Π΄Π΅Π»ΠΈ β Π² 8,8 ΡΠ°Π·Π°.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ Π½ΠΈΠ·ΠΊΠΎΠ΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ ΡΡΠΎΠ²Π½Ρ ΡΠΈΡΠΎΡ
ΡΠΎΠΌΠ° Π‘ Π² ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΡΡ
ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ ΠΏΡΠΈ ΡΡΠ°Π½Π΄Π°ΡΡΠ½ΠΎΠΌ ΠΈ ΡΡΠΈΠΌΡΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΌ ΡΠΎΡΡΠ΅ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ Π΄Π°Π½Π½ΡΠ΅ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°ΡΡ Π² ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ΅ ΠΈ ΠΊΠ»ΠΈΠ½ΠΈΠΊΠ΅ ΠΏΠΎ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ ΡΠΊΠ·ΠΎΠ³Π΅Π½Π½ΠΎΠ³ΠΎ ΡΠΈΡΠΎΡ
ΡΠΎΠΌΠ° Π‘ ΠΊΠ°ΠΊ Π°Π³Π΅Π½ΡΠ°, ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΡΡΡΠ΅Π³ΠΎ Π·Π°ΠΌΠ΅Π΄Π»Π΅Π½ΠΈΡ Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ°