The Organizational Effects of Oxytocin on the Central Expression of Estrogen Receptor Alpha and Oxytocin in Adulthood

Background: Previous studies have demonstrated that neonatal manipulation of oxytocin (OT) has effects on the expression of estrogen receptor alpha (ER alpha) and the central production of oxytocin observed in juveniles ( at weaning, 21 days of age). The goal of this study was to determine whether the effects of neonatal manipulation of OT last into adulthood, and if the effects differ from those observed during the early postnatal period. On the first day of life, prairie voles ( Microtus ochrogaster) received one of three doses of OT (High, 3 mu g; Med, 0.3 mu g; Low, 0.03 mu g), an OT antagonist, or isotonic saline. Another group was handled, but not injected. Then as adults, brains were collected, sectioned, and stained for ERa or OT using immunocytochemistry. Results: In females, treatment with OT increased the expression of ERa immunoreactivity in the ventral lateral septum (0.03 mu g) and the ventromedial nucleus of the hypothalamus and central amygdala ( 0.3 g). In males, OT antagonist increased ERa expression in the bed nucleus of the stria terminalis. There was no apparent effect of OT on the number of cells producing OT in the paraventricular nucleus of the hypothalamus. Conclusion: The current results suggest that neonatal manipulation of OT has long-term organizational effects on the expression of ERa in both males and females. The lack of effect on OT neurons in the paraventricular nucleus suggests that some developmental effects of OT previously observed in weanlings do not persist into adulthood. Developmental effects of OT on ERa patterns were sexually dimorphic, dose-dependent, and site-specific

The organizational effects of oxytocin on the central expression of estrogen receptor α and oxytocin in adulthood

Background: Previous studies have demonstrated that neonatal manipulation of oxytocin (OT) has effects on the expression of estrogen receptor alpha (ER alpha) and the central production of oxytocin observed in juveniles ( at weaning, 21 days of age). The goal of this study was to determine whether the effects of neonatal manipulation of OT last into adulthood, and if the effects differ from those observed during the early postnatal period. On the first day of life, prairie voles ( Microtus ochrogaster) received one of three doses of OT (High, 3 mu g; Med, 0.3 mu g; Low, 0.03 mu g), an OT antagonist, or isotonic saline. Another group was handled, but not injected. Then as adults, brains were collected, sectioned, and stained for ERa or OT using immunocytochemistry. Results: In females, treatment with OT increased the expression of ERa immunoreactivity in the ventral lateral septum (0.03 mu g) and the ventromedial nucleus of the hypothalamus and central amygdala ( 0.3 g). In males, OT antagonist increased ERa expression in the bed nucleus of the stria terminalis. There was no apparent effect of OT on the number of cells producing OT in the paraventricular nucleus of the hypothalamus. Conclusion: The current results suggest that neonatal manipulation of OT has long-term organizational effects on the expression of ERa in both males and females. The lack of effect on OT neurons in the paraventricular nucleus suggests that some developmental effects of OT previously observed in weanlings do not persist into adulthood. Developmental effects of OT on ERa patterns were sexually dimorphic, dose-dependent, and site-specific

Typologies of stress appraisal and problem-focused coping: associations with compliance with public health recommendations during the COVID-19 pandemic.

BACKGROUND: Given prior research finding that young adults are less likely to engage in recommended public health behaviors (PHBs) than older adults, understanding who is and is not likely to engage in PHBs among young adults is crucial to mitigating the effects of the COVID-19 pandemic. Drawing on the Transactional Theory of Stress and Coping, this study examined how typologies of stress appraisal (SA) and problem-focused coping (PFC) among young adults were associated with compliance with public health recommendations during the pandemic. METHODS: An online sample of young adults in the United States, ages 18-35, was recruited during the early phase of the pandemic (April-May 2020). Participants reported their appraisals of how central, threatening, and uncontrollable the pandemic was, their tendencies to engage in instrumental, problem-focused coping strategies, and how frequently they engaged in three recommended PHBs (social distancing, mask wearing, and hand washing). RESULTS: Using latent class analysis, we identified three classes of individuals: Low-SA/Low-PFC, Low-SA/High-PFC, and High-SA/High-PFC. Demographics did not efficiently distinguish membership in the three classes. The former two classes reported less compliance with public health recommendations than did the latter class. Tests of measurement invariance for gender indicated trivial differences in the composition of class membership and relations to compliance. CONCLUSIONS: This research uncovered three qualitatively distinct classes of people who differed in their appraisal of the pandemic and their tendency to engage in PFC. Individuals who view the pandemic as central and threatening and engage in problem-focused coping were more likely than their peers to comply with guidelines recommending social distancing, mask wearing, and hand washing. These results contribute to our understanding of why people do and do not comply with public health guidelines and highlight the importance of attending to psychological variables in public health research. Understanding what drives poor compliance with public health recommendations can contribute to efforts promoting better compliance, and ultimately better health outcomes

Mechanisms of Pacific Summer Water variability in the Arctic's Central Canada Basin

© The Author(s), 2014. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Journal of Geophysical Research: Oceans 119 (2014): 7523–7548, doi:10.1002/2014JC010273.Pacific Water flows northward through Bering Strait and penetrates the Arctic Ocean halocline throughout the Canadian Basin sector of the Arctic. In summer, Pacific Summer Water (PSW) is modified by surface buoyancy fluxes and mixing as it crosses the shallow Chukchi Sea before entering the deep ocean. Measurements from Ice-Tethered Profilers, moorings, and hydrographic surveys between 2003 and 2013 reveal spatial and temporal variability in the PSW component of the halocline in the Central Canada Basin with increasing trends in integrated heat and freshwater content, a consequence of PSW layer thickening as well as layer freshening and warming. It is shown here how properties in the Chukchi Sea in summer control the temperature-salinity properties of PSW in the interior by subduction at isopycnals that outcrop in the Chukchi Sea. Results of an ocean model, forced by idealized winds, provide support to the mechanism of surface ocean Ekman transport convergence maintaining PSW ventilation of the halocline.Funding was provided by the National Science Foundation Division of Polar Programs under award 1107623, 1313614, 1107412, 1107277, 1303644, and 0938137 and by Yale University. ICMMG model development was supported by the Russian Fund for Basic Research (14-05-00730A)

Adenovirus-prime and baculovirus-boost heterologous immunization achieves sterile protection against malaria sporozoite challenge in a murine model.

With the increasing prevalence of artemisinin-resistant malaria parasites, a highly efficacious and durable vaccine for malaria is urgently required. We have developed an experimental virus-vectored vaccine platform based on an envelope-modified baculovirus dual-expression system (emBDES). Here, we show a conceptually new vaccine platform based on an adenovirus-prime/emBDES-boost heterologous immunization regimen expressing the Plasmodium falciparum circumsporozoite protein (PfCSP). A human adenovirus 5-prime/emBDES-boost heterologous immunization regimen consistently achieved higher sterile protection against transgenic P. berghei sporozoites expressing PfCSP after a mosquito-bite challenge than reverse-ordered or homologous immunization. This high protective efficacy was also achieved with a chimpanzee adenovirus 63-prime/emBDES-boost heterologous immunization regimen against an intravenous sporozoite challenge. Thus, we show that the adenovirus-prime/emBDES-boost heterologous immunization regimen confers sterile protection against sporozoite challenge by two individual routes, providing a promising new malaria vaccine platform for future clinical use

Enhanced Si and B diffusion in semiconductor-grade SiO 2 and the effect of strain on diffusion

Abstract We present experimental and simulation results of Si self-diffusion and B diffusion in SiO 2 formed directly on Si substrates by thermal oxidation. We show that both Si and B diffusion in SiO 2 are enhanced by SiO generated at the Si/SiO 2 interface and diffusing into SiO 2 . We also show that the existence of high-concentration B in SiO 2 enhances SiO diffusion, which enhances both Si self-diffusion and B diffusion. This correlated diffusion of Si and B in SiO 2 is consistent with the first-principles calculation results, which show that B diffuses via a complex of BSiO with frequent bond exchanges in the SiO 2 network. Furthermore, based on the results, the enhancement of Si self-diffusion and B diffusion in SiO 2 by compressive strain and their retardation by tensile strain are suggested.

Membrane Potential-Dependent Inactivation of Voltage-Gated Ion Channels in α-Cells Inhibits Glucagon Secretion From Human Islets

OBJECTIVE: To document the properties of the voltage-gated ion channels in human pancreatic alpha-cells and their role in glucagon release. RESEARCH DESIGN AND METHODS: Glucagon release was measured from intact islets. [Ca(2+)](i) was recorded in cells showing spontaneous activity at 1 mmol/l glucose. Membrane currents and potential were measured by whole-cell patch-clamping in isolated alpha-cells identified by immunocytochemistry. RESULT: Glucose inhibited glucagon secretion from human islets; maximal inhibition was observed at 6 mmol/l glucose. Glucagon secretion at 1 mmol/l glucose was inhibited by insulin but not by ZnCl(2). Glucose remained inhibitory in the presence of ZnCl(2) and after blockade of type-2 somatostatin receptors. Human alpha-cells are electrically active at 1 mmol/l glucose. Inhibition of K(ATP)-channels with tolbutamide depolarized alpha-cells by 10 mV and reduced the action potential amplitude. Human alpha-cells contain heteropodatoxin-sensitive A-type K(+)-channels, stromatoxin-sensitive delayed rectifying K(+)-channels, tetrodotoxin-sensitive Na(+)-currents, and low-threshold T-type, isradipine-sensitive L-type, and omega-agatoxin-sensitive P/Q-type Ca(2+)-channels. Glucagon secretion at 1 mmol/l glucose was inhibited by 40-70% by tetrodotoxin, heteropodatoxin-2, stromatoxin, omega-agatoxin, and isradipine. The [Ca(2+)](i) oscillations depend principally on Ca(2+)-influx via L-type Ca(2+)-channels. Capacitance measurements revealed a rapid (<50 ms) component of exocytosis. Exocytosis was negligible at voltages below -20 mV and peaked at 0 mV. Blocking P/Q-type Ca(2+)-currents abolished depolarization-evoked exocytosis. CONCLUSIONS: Human alpha-cells are electrically excitable, and blockade of any ion channel involved in action potential depolarization or repolarization results in inhibition of glucagon secretion. We propose that voltage-dependent inactivation of these channels underlies the inhibition of glucagon secretion by tolbutamide and glucose