Complex expression dynamics and robustness in C. elegans insulin networks

Gene families expand by gene duplication and resulting paralogs diverge through mutation. Functional diversification can include neo-functionalization as well as sub-functionalization of ancestral functions. In addition, redundancy in which multiple genes fulfill overlapping functions is often maintained. Here, we use the family of 40 Caenorhabditis elegans insulins to gain insight into the balance between specificity and redundancy. The insulin/insulin-like growth factor (IIS) pathway comprises a single receptor, DAF-2. To date, no single insulin-like peptide recapitulates all DAF-2-associated phenotypes, likely due to redundancy between insulin-like genes. To provide a first-level annotation of potential patterns of redundancy, we comprehensively delineate the spatiotemporal and conditional expression of all 40 insulins in living animals. We observe extensive dynamics in expression that can explain the lack of simple patterns of pair-wise redundancy. We propose a model in which gene families evolve to attain differential alliances in different tissues and in response to a range of environmental stresses

DNA barcoding confirmed the occurrence of invasive vegetable leaf miner, Liriomyza sativae Blanchard (Diptera:Agromyzidae) in Northeast India

The vegetable leaf miner, Liriomyza sativae (Diptera:Agromyzidae), is an invasive polyphagous species originally known to be found in America and now spread in many parts of Africa, Asia, and the Pacific region. During 2016, L. sativae was observed for the first time infesting tomato (Solanum lycopersicum L.) leaves in experimental farms of an institute at Umiam (Meghalaya state of northeastern India). Based on museum specimens, this species was reported from India on tomato during 1994. Nevertheless, no further information is hitherto available from India apart from just new record. Considering the pest status of L. sativae across the globe, it is crucial to understand its expansion range, severity, biological attributes, and seasonal incidence on tomato in India. Taxonomic identification of different species of Liriomyza leaf miners is very complex due to morphological resemblance, and consequently, species-level identification is often done incorrectly by mistaking one species for another. Therefore, we characterized L. sativae at the molecular level and developed species specific DNA barcodes by using mitochondrial cytochrome oxidase gene. Moreover, the information on the correct distribution, seasonal incidence, and basic biological attributes of different stages of L. sativae is reported and discussed

Synthesis, spectral characterization, theoretical, antimicrobial, DNA interaction and in vitro anticancer studies of Cu(II) and Zn(II) complexes with pyrimidine-morpholine based Schiff base ligand

Novel Cu(II) (1) and Zn(II) (2) complexes with 4-(1-(4-morpholinophenyl)ethylideneamino)pyrimidine-5-carbonitrile) (L) have been synthesized and characterized by various spectroscopic and analytical techniques. DFT (density functional theory) studies result confirms that, LMCT mechanism have been done between L and M(II) ions. The antimicrobial studies indicate that the ligand L and complexes 1 & 2 exhibit higher activity against the E. coli bacteria and C. albicans fungi. The groove binding mode of ligand L and complexes 1 & 2 with CT-DNA have been confirmed by electronic absorption, competitive binding, viscometric and cyclic voltammetric studies. The electronic absorption titration of ligand L and complexes 1 & 2 with DNA have been carried out in different buffer solutions (pH 4.0, 7.0 & 10.0). The Kb values of ligand L and complexes 1 & 2 are higher in acidic buffer at pH 4.0 (Kb = 2.42 × 105, L; 2.8 × 105, 1; 2.65 × 105, 2) and the results revealed that, the interaction of synthesized compounds with DNA were higher in the acidic medium than basic and neutral medium. Furthermore, CT-DNA cleavage studies of ligand L and complexes 1 & 2 have been studied. The in vitro anticancer activities results show that complexes 1 & 2 have moderate cytotoxicity against cancer cell lines and low toxicity on normal cell line than ligand L. Keywords: Pyrimidine, Morpholine, DFT, Antimicrobial, DNA binding, Anticancer studie