375 research outputs found
Fabrication and characterization of polyurethane foam prepared from liquefied oil palm mesocarp fibre with renewable monomer made from waste cooking oil
This study aims performance characteristics of polyurethane foams prepared by the reaction of biopolyol prepared from liquefied oil palm mesocarp fibre and renewable monomer with methylene diphenyl diisocyanate. The effect of prepared oil palm mesocarp biopolyol with incorporation of renewable monomer of PU foam on the thermal stability, mechanical properties and was analyzed by thermo gravimetric analysis, tensile and compressive tests and Fourier transform infrared spectroscopy. The improved thermal properties were achieved at a composition of oil palm mesocarp fibre foams (PMF). Oil PMF foams showed mechanical strength as compared to renewable monomer foams. PU foam prepared from oil palm mesocarp biopolyol with incorporation of renewable monomer improved the foams strength. An infrared spectroscopy study demonstrated the formation of urethane linkage
Dynamic mechanical analysis of Dwimatrix Biopolymer from waste oil blended with thermoplastic; LDPE/HDPE
This paper presents results from an experimental study on the dynamic mechanical and viscoelastic properties
of Biopolymer (BP) from Waste vegetable oils (WVO) blended with two types of thermoplastic polyethylene (PE) which
are Low Density Polyethylene (LDPE) and High Density Polyethylene (HDPE). BP blended containing 10–30wt.% of BP
were injection moulded. MDI were used as crosslinker agent for modifying the matrices. Dynamic mechanical analysis
(DMA) of the blended were performed over a temperature range of 30–120°C for BL and 30-140°C for BH under frequency
of 1 Hz. DMA revealed no noticeable changes in a-transition temperature when the BP content was increased or were
added. The BP blended revealed better temperature resistance at higher BP content. However, the increase in storage
modulus was negligible at BL10 and BH20; due to the agglomeration of the BP. The results of the damping ratio analysis
revealed that higher interfacial bonding was achieved by addition of MDI crosslinker agent
BIODIESEL PRODUCTION FROM WASTE COOKING OIL BY USING ULTRASONIC TUBULAR REACTOR
The aim of this research is to find an optimum of synthesis biodiesel from waste cooking oil (WCO) using ultrasonic tubular reactor. The experimental studies explored the variations in reaction time, molar ratio WCO to methanol (MeOH), amount of catalyst, frequency of ultrasonic and output power ultrasonic on the ester contents. Comparisons of type ultrasonic and also mechanical stirring method based on time reaction were investigated. The optimum results of biodiesel process is the reaction time of 5 minute, NaOH catalyst 1%wt of WCO, molar ratio WCO to MeOH of 1:6, frequency ultrasonic of 20 KHz and output power ultrasonic of 650 W. The reaction time reduced 12-24 times compared to both of method and the yield of ester contents was obtained at 96.54%wt
BIODIESEL PRODUCTION FROM WASTE COOKING OIL BY USING ULTRASONIC TUBULAR REACTOR
The aim of this research is to find an optimum of synthesis biodiesel from waste cooking oil (WCO) using ultrasonic tubular reactor. The experimental studies explored the variations in reaction time, molar ratio WCO to methanol (MeOH), amount of catalyst, frequency of ultrasonic and output power ultrasonic on the ester contents. Comparisons of type ultrasonic and also mechanical stirring method based on time reaction were investigated. The optimum results of biodiesel process is the reaction time of 5 minute, NaOH catalyst 1%wt of WCO, molar ratio WCO to MeOH of 1:6, frequency ultrasonic of 20 KHz and output power ultrasonic of 650 W. The reaction time reduced 12-24 times compared to both of method and the yield of ester contents was obtained at 96.54%wt
Dynamic mechanical analysis and morphology of petroleum-based and bio-epoxy foams with wood filler
Current challenges highlight the need for polymer research using renewable natural sources as a substitute for
petroleum-based polymers. In this study, consequently, the fabrication of green polyurethane (PU) foams and its composites
is to be demonstrated dependent on synthesis in the laboratory scale of hydroxylated bio-epoxy (B) and petroleum-based
synthetic-epoxy (E), crosslinker and wood fillers. Polyurethane foams were modified with two type of wood fiber fillers,
powder (P) and flakes (L) with specific percentage ratios of 0, 5, 10, 15 and 20 %wt. Bio-epoxy (B) and synthetic-epoxy
(E) foam and its composite were exposed to UV irradiation for a period of 2000 hours and 4000 hours by UV
Whetherometer apparatus. The morphology structure and viscoelastic properties such as storage modulus, E', damping
behavior, ta
Study of tensile properties for aluminum 6061 reinforced with AISI 1060 steel machining chips
The present study focuses on the tensile properties of Aluminum 6061 reinforced with AISI1060 Steel
machining chips. Three different types of samples of aluminium 6061 reinforced with AISI 1060 steel machining
chips were prepared using stir casting. The tensile test was conducted using universal test machine (UTM) for
fabricated composites to study the tensile properties of aluminium 6061 reinforced with steel machining chips AISI
1060.The samples were prepared with different composition of AISI 1060 steel machining chip of 5%, 10% and
15% according to the weight fraction of parameter variable. Two casting processes were applied in this project
namely stir casting and sand casting process. The stir casting was used to mix the two different metals to become a
composite at temperature between 750℃ to 800℃ in a furnace while sand casting was used to fabricate the
samples into ASTM-E8M standard for tensile testing. The results showed that the samples with higher
reinforcement percentages of steel machining (15%) showed higher tensile stress and higher modulus value
characteristic compared to other samples. The experiment proved that the reinforcement material has good
mechanical properties compared to the pure material
Tensile property of melt mixing co-polypropylene with waste polymer at different composition ratios
In order to implement economical way of managing unwanted substances, waste polymer (WP) can be recycled instead of throwing it away. It can be reused via "sink-float" technique and mixed with another binder like co-polypropylene (co-PP) to enhance the mechani-cal properties of the material. This is because co-PP is a bit softer but has better impact strength, much tougher and more durable than polypropylene (PP). Two materials underwent injection moulding with 170°C temperature to produce dog bone samples for tensile test. The test started with 10% up until 80% of WP content mixed with co-PP. Tensile test speed used for this experiment was 5mm/s. Stress vs. strain graph was obtained from the test and the modulus of elasticity was obtained by using the stress over strain formula. The stress versus strain result for the composite with WP is lower than that of co-PP, proving that WP is more brittle compared to the more elastic co-PP. Morphological analysis of surface structure based on Optical Microscope (OM) indicates that co-PP has smooth surface while WP has a rough surface
Molecular Analysis Of Cyp21 Gene In Patients Presenting With Ambiguous Genitalia.
Congenital adrenal hyperplasia (CAH) is a group of autosomal recessive disorders of adrenal steroidogenesis
Antisense PMO Found in Dystrophic Dog Model Was Effective in Cells from Exon 7-Deleted DMD Patient
BACKGROUND: Antisense oligonucleotide-induced exon skipping is a promising approach for treatment of Duchenne muscular dystrophy (DMD). We have systemically administered an antisense phosphorodiamidate morpholino oligomer (PMO) targeting dystrophin exons 6 and 8 to a dog with canine X-linked muscular dystrophy in Japan (CXMD(J)) lacking exon 7 and achieved recovery of dystrophin in skeletal muscle. To date, however, antisense chemical compounds used in DMD animal models have not been directly applied to a DMD patient having the same type of exon deletion. We recently identified a DMD patient with an exon 7 deletion and tried direct translation of the antisense PMO used in dog models to the DMD patient's cells. METHODOLOGY/PRINCIPAL FINDINGS: We converted fibroblasts of CXMD(J) and the DMD patient to myotubes by FACS-aided MyoD transduction. Antisense PMOs targeting identical regions of dog and human dystrophin exons 6 and 8 were designed. These antisense PMOs were mixed and administered as a cocktail to either dog or human cells in vitro. In the CXMD(J) and human DMD cells, we observed a similar efficacy of skipping of exons 6 and 8 and a similar extent of dystrophin protein recovery. The accompanying skipping of exon 9, which did not alter the reading frame, was different between cells of these two species. CONCLUSION/SIGNIFICANCE: Antisense PMOs, the effectiveness of which has been demonstrated in a dog model, achieved multi-exon skipping of dystrophin gene on the FACS-aided MyoD-transduced fibroblasts from an exon 7-deleted DMD patient, suggesting the feasibility of systemic multi-exon skipping in humans
Targeted Skipping of Human Dystrophin Exons in Transgenic Mouse Model Systemically for Antisense Drug Development
Antisense therapy has recently been demonstrated with great potential for targeted exon skipping and restoration of dystrophin production in cultured muscle cells and in muscles of Duchenne Muscular Dystrophy (DMD) patients. Therapeutic values of exon skipping critically depend on efficacy of the drugs, antisense oligomers (AOs). However, no animal model has been established to test AO targeting human dystrophin exon in vivo systemically. In this study, we applied Vivo-Morpholino to the hDMD/mdx mouse, a transgenic model carrying the full-length human dystrophin gene with mdx background, and achieved for the first time more than 70% efficiency of targeted human dystrophin exon skipping in vivo systemically. We also established a GFP-reporter myoblast culture to screen AOs targeting human dystrophin exon 50. Antisense efficiency for most AOs is consistent between the reporter cells, human myoblasts and in the hDMD/mdx mice in vivo. However, variation in efficiency was also clearly observed. A combination of in vitro cell culture and a Vivo-Morpholino based evaluation in vivo systemically in the hDMD/mdx mice therefore may represent a prudent approach for selecting AO drug and to meet the regulatory requirement
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