Implicitly Constrained Semi-Supervised Least Squares Classification

We introduce a novel semi-supervised version of the least squares classifier. This implicitly constrained least squares (ICLS) classifier minimizes the squared loss on the labeled data among the set of parameters implied by all possible labelings of the unlabeled data. Unlike other discriminative semi-supervised methods, our approach does not introduce explicit additional assumptions into the objective function, but leverages implicit assumptions already present in the choice of the supervised least squares classifier. We show this approach can be formulated as a quadratic programming problem and its solution can be found using a simple gradient descent procedure. We prove that, in a certain way, our method never leads to performance worse than the supervised classifier. Experimental results corroborate this theoretical result in the multidimensional case on benchmark datasets, also in terms of the error rate.Comment: 12 pages, 2 figures, 1 table. The Fourteenth International Symposium on Intelligent Data Analysis (2015), Saint-Etienne, Franc

Puromycin Sensitivity of Ribosomal Label after Incorporation of 14C-Labelled Amino Acids into Isolated Mitochondria from Neurospora crassa

Radioactive amino acids were incorporated into isolated mitochondria from Neurospora crassa. Then the mitochondrial ribosomes were isolated and submitted to density gradient centrifugation. A preferential labelling of polysomes was observed. However, when the mitochondrial suspension was treated with puromycin after amino acid incorporation, no radioactivity could be detected in either the monosomes or the polysomes. The conclusion is drawn that isolated mitochondria under these conditions do not incorporate significant amounts of amino acids into proteins of their ribosomes

A Unifying View of Multiple Kernel Learning

Recent research on multiple kernel learning has lead to a number of approaches for combining kernels in regularized risk minimization. The proposed approaches include different formulations of objectives and varying regularization strategies. In this paper we present a unifying general optimization criterion for multiple kernel learning and show how existing formulations are subsumed as special cases. We also derive the criterion's dual representation, which is suitable for general smooth optimization algorithms. Finally, we evaluate multiple kernel learning in this framework analytically using a Rademacher complexity bound on the generalization error and empirically in a set of experiments

Foundation and empire : a critique of Hardt and Negri

In this article, Thompson complements recent critiques of Hardt and Negri's Empire (see Finn Bowring in Capital and Class, no. 83) using the tools of labour process theory to critique the political economy of Empire, and to note its unfortunate similarities to conventional theories of the knowledge economy

Precursors of Cytochrome Oxidase in Cytochrome-Oxidase-Deficient Cells of Neurospora crassa

Three different cell types of Neurospora crassa deficient in cytochrome oxidase were studied: the nuclear mutant cni-1, the cytoplasmic mutant mi-1 and copper-depleted wild-type cells. * 1. The enzyme-deficient cells have retained a functioning mitochondrial protein synthesis. It accounted for 12–16% of the total protein synthesis of the cell. However, the analysis of mitochondrial translation products by gel electrophoresis revealed that different amounts of individual membrane proteins were synthesized. Especially mutant cni-1 produced large amounts of a small molecular weight translation product, which is barely detectable in wild-type. * 2. Mitochondrial preparations of cytochrome-oxidase-deficient cells were examined for precursors of cytochrome oxidase. The presence of polypeptide components of cytochrome oxidase in the mitochondria was established with specific antibodies. On the other hand, no significant amounts of heme a could be extracted. * 3. Radioactively labelled components of cytochrome oxidase were isolated by immunoprecipitation and analysed by gel electrophoresis. All three cell types contained the enzyme components 4–7, which are translated on cytoplasmic ribosomes. The mitochondrially synthesized components 1–3 were present in mi-1 mutant and in copper-depleted wild-type cells. In contrast, components 2 and 3 were not detectable in the nuclear mutant cni-1. Both relative and absolute amounts of these polypeptides in the enzyme-deficient cells were quite different from those in wild-type cells. * 4. The components of cytochrome oxidase found in the enzyme-deficient cells were tightly associated with the mitochondrial membranes. * 5. Processes, which affect and may control the production of enzyme precursors or their assembly to a functional cytochrome oxidase are discussed

Design with a Positive Lens: An affirmative approach to designing information and organizations

Design forms one critical paradigmatic view that pervades organizational studies, management, and information systems research. Building on the discussions in the First Working Conference on Designing Information and Organizations with a Positive Lens, we chart the potential contribution of positive design to the shaping of organizations, work processes, artifacts, communication networks, and information technologies. The figure of speech "Design with a Positive Lens," or in short, "Positive Design," connotes here a distinctive perspective on design that is less focused on the detection of errors associated with gaining control and more concerned with human-centered design associated with the shaping of hopeful organizations and a thriving future. The paper examines how positive design can contribute to the design of information systems and organizations as related to five broad-scale areas: design of high performance work processes; positive design methods and techniques: cooperation and collaboration across boundaries to promote positive change; positive organizational design, and design science and practice. In this paper we aspire to promote the emerging cross-disciplinary discourse between scholars and designers that will foster positive organizational and technological design

Low-frequency characterization of quantum tunneling in flux qubits

We propose to investigate flux qubits by the impedance measurement technique (IMT), currently used to determine the current--phase relation in Josephson junctions. We analyze in detail the case of a high-quality tank circuit coupled to a persistent-current qubit, to which IMT was successfully applied in the classical regime. It is shown that low-frequency IMT can give considerable information about the level anticrossing, in particular the value of the tunneling amplitude. An interesting difference exists between applying the ac bias directly to the tank and indirectly via the qubit. In the latter case, a convenient way to find the degeneracy point in situ is described. Our design only involves existing technology, and its noise tolerance is quantitatively estimated to be realistic.Comment: 6 pages, 11 figures, to appear in Phys.Rev.

Communications Biophysics

Contains reports on four research projects.National Institutes of Health (Grant 5 PO1 GM14940-05)National Aeronautics and Space Administration (Grant NGL 22-009-304)National Institutes of Health (Grant 5 TO1 GM01555-05)Boston City Hospital Purchase Order 10656B-D Electrodyne Division, Becton Dickinson and Compan