2 research outputs found
Effective Phone Calls
“Effective Phone Calls” is a practical and accessible course specifically designed to develop students` essential communication and language skills. It is divided into six units, which contain face-to-face with teacher and self-study materials. The course aims to develop both competence and confi-dence in a variety of situations, so that by the end of the period of study students will have acquired the necessary skills to handle almost any kind of call in business setting
Identification and Characterization of a Secondary Sodium-Binding Site and the Main Selectivity Determinants in the Human Concentrative Nucleoside Transporter 3
The family of concentrative Na<sup>+</sup>/nucleoside cotransporters
in humans is constituted by three subtypes, namely, hCNT1, hCNT2,
and hCNT3. Besides their different nucleoside selectivity, hCNT1 and
hCNT2 have a Na<sup>+</sup>/nucleoside stoichiometry of 1:1, while
for hCNT3 it is 2:1. This distinct stoichiometry of subtype 3 might
hint the existence of a secondary sodium-binding site that is not
present in the other two subtypes, but to date their three-dimensional
structures remain unknown and the residues implicated in Na<sup>+</sup> binding are unclear. In this work, we have identified and characterized
the Na<sup>+</sup> binding sites of hCNT3 by combining molecular modeling
and mutagenesis studies. A model of the transporter was obtained by
homology modeling, and key residues of two sodium-binding sites were
identified and verified with a mutagenesis strategy. The structural
model explains the altered sodium-binding properties of the hCNT3C602R
polymorphic variant and supports previously generated data identifying
the determinant residues of nucleoside selectivity, paving the way
to understand how drugs can target this plasma membrane transporter