12 research outputs found
Polyphenols of <i>Perilla frutescens</i> of the family Lamiaceae identified by tandem mass spectrometry
Perilla frutescens is mainly cultivated as an oilseed crop. Perilla seeds contain 40β53 % of oil, 28 % of protein. The growing season is 100β150 days. In Russia, perilla is grown in the Far East, where the yield is 0.8β1.2 t/ha. Perilla of different geographical origin has its own special, sharply different features that characterize two geographical groups: Japanese and Korean-Chinese. These groups differ from each other in the length of the growing season, the height of plants, the color of the stem, the surface and the size of the leaves, the shape of the bush, the shape and size of the inflorescences, the size of the cups, the size and color of the seeds. P. frutescens contains a large number of polyphenolic compounds that are biologically active components. The purpose of this research was a metabolomic study of extracts from leaves of P. frutescens obtained from the collection of Federal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources, grown on the fields of the Far East Experiment Station β Branch of Federal Research Center (Primorsky Krai, Russia). To identify target analytes in extracts, HPLC was used in combination with an ion trap. Preliminary results showed the presence of 23 biologically active compounds corresponding to P. frutescens. In addition to the reported metabolites, a number of metabolites were newly annotated in P. frutescens. There were hydroxycoumarin Umbelliferone; triterpene Squalene; omega-3 fatty acid Stearidonic [Moroctic] acid; higher-molecular-weight carboxylic acid: Tetracosenoic acid and Salvianic acid C; lignan Syringaresinol and cyclobutane lignan Sagerinic acid, etc. A wide range of biologically active compounds opens up rich opportunities for the creation of new drugs and dietary supplements based on extracts of perilla of the family Lamiaceae, subfamily Lamioideae, tribe Satureji and subtribe Perillinae
ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΠ»ΠΎΠ΄ΠΎΠ² ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² ΡΠΎΠΌΠ°ΡΠ° Solanum lycopersicum L., ΠΏΡΠΎΠΈΡΡ ΠΎΠ΄ΡΡΠΈΡ ΠΈΠ· ΡΠ°Π·Π½ΡΡ ΠΊΠ»ΠΈΠΌΠ°ΡΠΎ-Π³Π΅ΠΎΠ³ΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΡ Π·ΠΎΠ½, ΠΈ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΠΊΠ°ΡΠΈΡ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠΎΠ² ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠ°Π½Π΄Π΅ΠΌΠ½ΠΎΠΉ ΠΌΠ°ΡΡ-ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠΈΠΈ
Relevance. A number of scientific studies confirm that consumption of fruits and vegetables can reduce the risk of certain chronic diseases, such as cancer and cardiovascular diseases, for example, consumption of fresh tomatoes and tomato products is inversely proportional to the development of certain types of cancer. Tomato Solanum lycopersicum L. contains a large number of polyphenolic complexes, which are biologically active compounds. In this article, the authors have attempted for the first time to present the complete metabolomic composition of Solanum lycopersicum extracts.Materials and methods: As an object of research, authors used the extracts of Solanum lycopersicum L., from the collection of the Federal Research Center N.I. Vavilov All-Russian Institute of Plant Genetic Resources, grown and collected at the Far Eastern Experiment Station Branch of the Federal State Budgetary Scientific Institution in September 2020 (varieties: k-5351 Ont77 13, Canada; k-3149 Rehovoth, Israel; 2698 Ukraine). High performance liquid chromatography (HPLC) in combination with a BRUKER DALTONIKS ion trap (tandem mass spectrometry) was used to identify target analytes in extracts obtained by the maceration method.Discussion: The results of initial studies revealed the presence of 36 biologically active compounds, of which 22 were identified for the first time in Solanum lycopersicum L. These are Apigenin, Luteolin, Kaempferol, Taxifolin, Myricetin, Coutaric acid, Caffeoylmalic acid, Caftaric acid, Dicaffeoylquinic acid, coumarins Fraxetin, and Fraxetin-7-O-beta-glucuronide, Pelargonidin, Salvianolic acid D, Rosmanol, Colnelenic acid, Ethyl rosemary, lignan Medioresinol-O-hexoside, Squalene, etc. The findings will help to intensify future research into the development and production of various functional food products containing targeted extracts of Solanum lycopersicum L.ΠΠΊΡΡΠ°Π»ΡΠ½ΠΎΡΡΡ. Π¦Π΅Π»ΡΠΉ ΡΡΠ΄ Π½Π°ΡΡΠ½ΡΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π°Π΅Ρ, ΡΡΠΎ ΠΏΠΎΡΡΠ΅Π±Π»Π΅Π½ΠΈΠ΅ ΡΡΡΠΊΡΠΎΠ² ΠΈ ΠΎΠ²ΠΎΡΠ΅ΠΉ ΠΌΠΎΠΆΠ΅Ρ ΡΠ½ΠΈΠ·ΠΈΡΡ ΡΠΈΡΠΊ Π½Π΅ΠΊΠΎΡΠΎΡΡΡ
Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΈΡ
Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ, ΡΠ°ΠΊΠΈΡ
ΠΊΠ°ΠΊ ΡΠ°ΠΊ ΠΈ ΡΠ΅ΡΠ΄Π΅ΡΠ½ΠΎ- ΡΠΎΡΡΠ΄ΠΈΡΡΡΠ΅ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ, Π½Π°ΠΏΡΠΈΠΌΠ΅Ρ, ΠΏΠΎΡΡΠ΅Π±Π»Π΅Π½ΠΈΠ΅ ΡΠ²Π΅ΠΆΠΈΡ
ΠΏΠΎΠΌΠΈΠ΄ΠΎΡΠΎΠ² ΠΈ ΠΏΡΠΎΠ΄ΡΠΊΡΠΎΠ² ΠΈΠ· ΡΠΎΠΌΠ°ΡΠΎΠ² ΠΎΠ±ΡΠ°ΡΠ½ΠΎ ΠΏΡΠΎΠΏΠΎΡΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎ ΡΠ°Π·Π²ΠΈΡΠΈΡ Π½Π΅ΠΊΠΎΡΠΎΡΡΡ
Π²ΠΈΠ΄ΠΎΠ² ΡΠ°ΠΊΠ°. ΠΠ»ΠΎΠ΄Ρ ΡΠΎΠΌΠ°ΡΠ° Solanum lycopersicumL. ΡΠΎΠ΄Π΅ΡΠΆΠ°Ρ Π±ΠΎΠ»ΡΡΠΎΠ΅ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ ΠΏΠΎΠ»ΠΈΡΠ΅Π½ΠΎΠ»ΡΠ½ΡΡ
ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΠ², ΡΠ²Π»ΡΡΡΠΈΡ
ΡΡ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈ Π°ΠΊΡΠΈΠ²Π½ΡΠΌΠΈ ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΡΠΌΠΈ. Π Π΄Π°Π½Π½ΠΎΠΉ ΡΡΠ°ΡΡΠ΅ Π°Π²ΡΠΎΡΡ Π²ΠΏΠ΅ΡΠ²ΡΠ΅ ΠΏΠΎΠΏΡΡΠ°Π»ΠΈΡΡ ΠΏΡΠ΅Π΄ΡΡΠ°Π²ΠΈΡΡ ΠΏΠΎΠ»Π½ΡΠΉ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΎΠΌΠ½ΡΠΉ ΡΠΎΡΡΠ°Π² ΡΠΊΡΡΡΠ°ΠΊΡΠΎΠ² ΠΏΠ»ΠΎΠ΄ΠΎΠ² Solanum lycopersicum.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ: Π ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΠΎΠ±ΡΠ΅ΠΊΡΠ° ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π±ΡΠ»ΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Ρ ΠΏΠ»ΠΎΠ΄Ρ ΡΠΎΠΌΠ°ΡΠΎΠ² Solanum lycopersicum L. ΠΈΠ· ΠΊΠΎΠ»Π»Π΅ΠΊΡΠΈΠΈ ΠΡΠ΅ΡΠΎΡΡΠΈΠΉΡΠΊΠΎΠ³ΠΎ ΠΈΠ½ΡΡΠΈΡΡΡΠ° Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ΅ΡΡΡΡΠΎΠ² ΠΈΠΌ. Π.Π. ΠΠ°Π²ΠΈΠ»ΠΎΠ²Π°, Π²ΡΡΠ°ΡΠ΅Π½Π½ΡΠ΅ ΠΈ ΡΠΎΠ±ΡΠ°Π½Π½ΡΠ΅ Π½Π° ΠΠ°Π»ΡΠ½Π΅Π²ΠΎΡΡΠΎΡΠ½ΠΎΠΉ ΠΎΠΏΡΡΠ½ΠΎΠΉ ΡΡΠ°Π½ΡΠΈΠΈ Π€ΠΈΠ»ΠΈΠ°Π»Π΅ ΠΠΠ Π² ΡΠ΅Π½ΡΡΠ±ΡΠ΅ 2020 Π³ΠΎΠ΄Π° (ΡΠΎΡΡΠ°: ΠΊ-5351 Ont77 13, ΠΠ°Π½Π°Π΄Π°; ΠΊ-3149 Rehovoth, ΠΠ·ΡΠ°ΠΈΠ»Ρ; 2698 Π£ΠΊΡΠ°ΠΈΠ½Π°). ΠΠ»Ρ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ ΡΠ΅Π»Π΅Π²ΡΡ
Π°Π½Π°Π»ΠΈΡΠΎΠ² Π² ΡΠΊΡΡΡΠ°ΠΊΡΠ°Ρ
, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ
ΠΌΠ°ΡΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΡΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ, ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Π° Π²ΡΡΠΎΠΊΠΎΡΡΡΠ΅ΠΊΡΠΈΠ²Π½Π°Ρ ΠΆΠΈΠ΄ΠΊΠΎΡΡΠ½Π°Ρ Ρ
ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΠΈΡ (ΠΠΠΠ₯) Π² ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ΅ Ρ ΠΈΠΎΠ½Π½ΠΎΠΉ Π»ΠΎΠ²ΡΡΠΊΠΎΠΉ BRUKER DALTONIKS (ΡΠ°Π½Π΄Π΅ΠΌΠ½Π°Ρ ΠΌΠ°ΡΡ-ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠΈΡ).Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ: Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ Π½Π°ΡΠ°Π»ΡΠ½ΡΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ Π²ΡΡΠ²ΠΈΠ»ΠΈ ΠΏΡΠΈΡΡΡΡΡΠ²ΠΈΠ΅ 36 ΠΏΠΎΠ»ΠΈΡΠ΅Π½ΠΎΠ»ΡΠ½ΡΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ ΠΈ ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ Π΄ΡΡΠ³ΠΈΡ
ΠΊΠ»Π°ΡΡΠΎΠ², ΠΈΠ· Π½ΠΈΡ
22 ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½ΠΎ Π²ΠΏΠ΅ΡΠ²ΡΠ΅ Π² Solanum lycopersicumL. ΠΡΠΎ Π°ΠΏΠΈΠ³Π΅Π½ΠΈΠ½, Π»ΡΡΠ΅ΠΎΠ»ΠΈΠ½, ΠΊΠ°ΠΌΠΏΡΠ΅ΡΠΎΠ», ΡΠ°ΠΊΡΠΈΡΠΎΠ»ΠΈΠ½, ΠΌΠΈΡΠΈΡΠ΅ΡΠΈΠ½, ΠΊΡΡΠ°ΡΠΎΠ²Π°Ρ ΠΊΠΈΡΠ»ΠΎΡΠ°, ΠΊΠΎΡΠ΅ΠΈΠ»ΠΌΠ°Π»Π΅Π²Π°Ρ ΠΊΠΈΡΠ»ΠΎΡΠ°, ΠΊΠ°ΡΡΠ°ΡΠΎΠ²Π°Ρ ΠΊΠΈΡΠ»ΠΎΡΠ°, Π΄ΠΈΠΊΠ°ΡΡΠ΅ΠΎΠΈΠ»Ρ
ΠΈΠ½ΠΎΠ²Π°Ρ ΠΊΠΈΡΠ»ΠΎΡΠ°, ΠΊΡΠΌΠ°ΡΠΈΠ½Ρ ΡΡΠ°ΠΊΡΠ΅ΡΠΈΠ½ ΠΈ Π³Π»ΡΠΊΠΎΡΠΎΠ½ΠΈΠ΄ ΡΡΠ°ΠΊΡΠ΅ΡΠΈΠ½Π°, Π°Π½ΡΠΎΡΠΈΠ°Π½ΠΈΠ½ ΠΏΠ΅Π»Π°ΡΠ³ΠΎΠ½ΠΈΠ΄ΠΈΠ½, ΡΠ°Π»ΡΠ²ΠΈΠ°Π½ΠΎΠ»ΠΎΠ²Π°Ρ ΠΊΠΈΡΠ»ΠΎΡΠ° D, ΡΠΎΠ·ΠΌΠ°Π½ΠΎΠ», ΠΊΠΎΠ»Π½Π΅Π»Π΅Π½ΠΎΠ²Π°Ρ ΠΊΠΈΡΠ»ΠΎΡΠ°, ΡΡΠΈΠ» ΡΠΎΠ·ΠΌΠ°ΡΠΈΠ½Π°Ρ, Π»ΠΈΠ³Π½Π°Π½ ΠΌΠ΅Π΄ΠΈΠΎΡΠ΅ΡΠΈΠ½ΠΎΠ», ΡΠΊΠ²Π°Π»Π΅Π½ ΠΈ Π΄Ρ. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ Π΄Π°Π½Π½ΡΠ΅ ΠΏΠΎΠΌΠΎΠ³ΡΡ ΠΈΠ½ΡΠ΅Π½ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°ΡΡ Π±ΡΠ΄ΡΡΠΈΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΏΠΎ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠ΅ ΠΈ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΡΡΠ²Ρ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΠΏΡΠΎΠ΄ΡΠΊΡΠΎΠ² ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΏΠΈΡΠ°Π½ΠΈΡ, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ
ΡΠ΅Π»Π΅Π²ΡΠ΅ ΡΠΊΡΡΡΠ°ΠΊΡΡ Solanum lycopersicumL
Research of 5 extracts of wild Amur grape
Vitis amurensis Ruprecht contains a large number of polyphenolic compounds which are biologically active components. For the most efficient and safe extraction supercritical carbon dioxide was used. In this work, for the first time, a comparative metabolomic study of biologically active substances of wild grapes collected from five different places of the Primorsky and Khabarovsk territories is carried out. To identify target analytes in ethanol extracts of grape berries, high performance liquid chromatography (HPLC) was used in combination with an amaZon SL ion trap (manufactured by BRUKER DALTONIKS, Germany) equipped with an ESI electrospray ionization source in negative and positive ion modes. The mass spectrometer was used in the scan range m / z 100 - 1.700 for MS and MS / MS. Used fragmentation of the 4th order. Primary mass spectrometric results showed the presence of 89 biologically active compounds corresponding to the species V. amurensis, moreover, salvianolic acids F, D and G, oleanoic, ursolic, myristoleic acids, berbericinin, mearnsetin, esculin, nevadensin, stigmasterol, fucosterol, phlorizin, L-tryptophan identified for the first time in V. amurensis
Simultaneous determination of polyphenol content
Vitis amurensis Ruprecht contains a large number of polyphenolic compounds which are biologically active components. For the most efficient and safe extraction supercritical carbon dioxide was used. In this work, for the first time, a comparative metabolomic study of biologically active substances of wild grapes collected from five different places of the Primorsky and Khabarovsk territories is carried out. To identify target analytes in ethanol extracts of grape berries, high performance liquid chromatography (HPLC) was used in combination with an amaZon SL ion trap (manufactured by BRUKER DALTONIKS, Germany) equipped with an ESI electrospray ionization source in negative and positive ion modes. The mass spectrometer was used in the scan range m / z 100 - 1.700 for MS and MS / MS. Used fragmentation of the 4th order. Primary mass spectrometric results showed the presence of 94 biologically active compounds corresponding to the species V. amurensis, moreover, salvianolic acids F, D and G, oleanoic, ursolic, myristoleic acids, berbericinin, mearnsetin, esculin, nevadensin, stigmasterol, fucosterol, phlorizin, L-tryptophan identified for the first time in V. amurensis
Comparison of Wild and Introduced <i>Dracocephalum jacutense</i> P.: Significant Differences of Multicomponent Composition
Dracocephalum jacutense is endemic to eastern Siberia of Russia and is accepted in the rare and endangered category. The plant was first collected by K.S. Baikov in 1985 in the vicinity of the village Sangar (Kobyaysky district, Yakutia) and then described by G.A. Peshkova in βFlora of Siberiaβ in 1997. D. jacutense has been introduced in the Botanical Garden of Yakutia since 2009. The aim of this work is to conduct a comparative analysis of the chemical composition of aerial parts (leaves, inflorescences, stems) of D. jacutense Peschkova collected both in controlled conditions (the Botanical Garden of Yakutia) and in a natural-grown area (the vicinity of the village of Sangar, Kobyaysky district of Yakutia). A total of 156 bioactive compounds were successfully characterized in extracts of D. jacutense based on their accurate MS (Mass Spectrometry) fragment ions by searching online databases and the reported literature. The detailed study of the composition by tandem mass spectrometry revealed a significant difference in the polyphenol composition of the samples. Wild-grown plant samples had a higher number of polyphenolic compounds (92 compounds) than plant samples grown in the Botanical Garden (56 compounds), which were not previously described in the genus Dracocephalum. In addition, a total of 37 compounds of other chemical groups were identified that were not previously identified in the genus Dracocephalum. In general, the extract of D. jacutense, which was grown in wild conditions, was found to be a richer source of flavones, flavanols, flavan-3-ols, phenolic acids, and anthocyanidins compared to plants grown in controlled conditions in the Botanical Garden. Our results build on the current understanding of the biochemical richness of wild-grown samples over controlled-grown ones and preserve a rare and endangered D. jacutense in the flora of Yakutia. We proposed to be preserved on the basis of the development of an in vitro micropropagation protocol in our lab in the near future
Zostera marina L.: Supercritical CO2-extraction and mass spectrometric characterization of chemical constituents recovered from seagrass
Three types of Zostera marina L. collection were extracted using the supercritical CO2-extraction method. For the purposes of supercritical CO2-extraction, old seagrass ejection on the surf edge, fresh seagrass ejection on the surf edge and seagrass collected in water were used. Several experimental conditions were investigated in the pressure range 50-350 bar, with the used volume of co-solvent ethanol in the amount of 1% in the liquid phase at a temperature in the range of 31-70 degrees C. The most effective extraction conditions are: pressure 250 Bar and temperature 60 degrees C for Z. marina collected in sea water. Z. marina contain various phenolic compounds and sulfated polyphenols with valuable biological activity. Tandem mass-spectrometry (HPLC-ESI-ion trap) was applied to detect target analytes. 77 different biologically active components have been identified in Z. marina supercritical CO2-extracts. 38 polyphenols were identified for the first time in Z. marina
Dracocephalum palmatum S. and Dracocephalum ruyschiana L. originating from Yakutia : a high-resolution mass spectrometric approach for the comprehensive characterization of phenolic compounds
Dracocephalum palmatum S. and Dracocephalum ruyschiana L. contain a large number of target analytes, which are biologically active compounds. High performance liquid chromatography (HPLC) in combination with an ion trap (tandem mass spectrometry) was used to identify target analytes in extracts of D. palmatum S. and D. ruyschiana L. originating from Yakutia. The results of initial studies revealed the presence of 114 compounds, of which 92 were identified for the first time in the genus Dracocephalum. New identified metabolites belonged to 17 classes, including 16 phenolic acids and their conjugates, 18 flavones, 5 flavonols, 2 flavan-3-ols, 1 flavanone, 2 stilbenes, 10 anthocyanins, 1 condensed tannin, 2 lignans, 6 carotenoids, 3 oxylipins, 2 amino acids, 3 sceletium alkaloids, 3 carboxylic acids, 8 fatty acids, 1 sterol, and 3 terpenes, along with 6 miscellaneous compounds. It was shown that extracts of D. palmatum are richer in the spectrum of polyphenolic compounds compared with extracts of D. ruyschiana, according to a study of the presence of these compounds in extracts, based on the results of mass spectrometric studies
Identification and Spatial Distribution of Bioactive Compounds in Seeds Vigna unguiculata (L.) Walp. by Laser Microscopy and Tandem Mass Spectrometry
The research presents a comparative metabolomic study of extracts of Vigna unguiculata seed samples from the collection of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources. Analyzed samples related to different areas of use in agricultural production, belonging to different cultivar groups sesquipedalis (vegetable accessions) and unguiculata (grain accessions). Metabolome analysis was performed by liquid chromatography combined with ion trap mass spectrometry. Substances were localized in seeds using confocal and laser microscopy. As a result, 49 bioactive compounds were identified: flavonols, flavones, flavan-3-ols, anthocyanidin, phenolic acids, amino acids, monocarboxylic acids, aminobenzoic acids, fatty acids, lignans, carotenoid, sapogenins, steroids, etc. Steroidal alkaloids were identified in V. unguiculata seeds for the first time. The seed coat (palisade epidermis and parenchyma) is the richest in phenolic compounds. Comparison of seeds of varieties of different directions of use in terms of the number of bioactive substances identified revealed a significant superiority of vegetable accessions over grain ones in this indicator, 36 compounds were found in samples from cultivar group sesquipedalis, and 24 in unguiculata. The greatest variety of bioactive compounds was found in the vegetable accession k-640 from China
Identification of phenolic constituents in
The purpose of this work was a comparative metabolomic study of extracts of Blueberried honeysuckle Lonicera caerulea L.: β1043-11 (St. Petersburg); β1043-08 (St. Petersburg) β863; (Japan); β860 (Wild Lonicera from Amur river) from the collection of N.I. Vavilov All-Russian Institute of Plant Genetic Resources. To identify target analytes in extracts HPLC was used in combination with a BRUKER DALTONIKS ion trap. The results showed the presence of 82 target analytes corresponding to family Caprifoliaceae. In addition to the reported metabolites, a number of metabolites were newly annotated in Lonicera caerulea L. There were flavonols: Dihydrokaempferol, Rhamnetin I, Rhamnetin II, Taxifolin-3O-glucoside, Mearnsetin-hexoside, Horridin; flavones: Chrysoeriol, Apigenin-O-pentoside, Chrysoeriol-7-Oglucoside; flavanone Naringenin; flavan-3-ols: Catechin, Epicatechin, Biochanin A-7-O-glucoside; essential amino acids: L-Pyroglutamic acid, Tyrosine; polypeptide 5-Oxo-L-propyl-L-isoleucine; sterols: Ergosterol, Fucosterol, Beta-Sitosterin; triterpenoids: Betunolic acid, Oleanoic acid; anabolic steroid Vebonol, indole sesquiterpene alkaloid Sespendole; iridoids: Monotropein, p-Coumaroyl monotropein, p-Coumaroyl monotropein hexoside; Myristoleic acid, etc