Coping with the pressure: Microbial adaptation to high hydrostatic pressure in deep sea hydrothermal vents

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Homeoviscous Adaptation of Membranes in Archaea

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Methanization of fossil fuel: a possible sustainable future energy source for mankind

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Corrigendum to “Caballeronia mineralivorans sp. nov., isolated from oak-Scleroderma citrinum mycorrhizosphere” [Syst. Appl. Microbiol. 40 (2017) 345–351]

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iGEM Europe 2013: Des gènes et des machines

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Adaptation of the membrane in Archaea

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Screening for N-AHSL-Based-Signaling Interfering Enzymes

Quorum sensing (QS)-based signaling is a widespread pathway used by bacteria for the regulation of functions involved in their relation to the environment or their host. QS relies upon the production, accumulation and perception of small diffusable molecules by the bacterial population, hence linking high gene expression with high cell population densities.Among the different QS signal molecules, an important class of signal molecules is the N-acyl homoserine lactone (N-AHSL). In pathogens such as Erwinia or Pseudomonas, N-AHSL based QS is crucial to overcome the host defenses and ensure a successful infection. Interfering with QS-regulation allows the algae Delisea pulcra to avoid surface colonization by bacteria.Thus, interfering the QS-regulation of pathogenic bacteria is a promising antibiotic-free antibacterial therapeutic strategy. To date, two N-AHSL lactonases and one amidohydrolase families of N-ASHL degradation enzymes have been characterized and have proven to be efficient in vitro to control N-AHSL-based QS-regulated functions in pathogens.In this chapter, we provide methods to screen individual clones or bacterial strains as well as pool of clones for genomic and metagenomic libraries, that can be used to identify strains or clones carrying N-ASHL degradation enzymes

Corrigendum to “Caballeronia mineralivorans sp. nov., isolated from oak-Scleroderma citrinum mycorrhizosphere” [Syst. Appl. Microbiol. 40 (2017) 345–351]

International audienc