Quality-controlled meteorological datasets from SIGMA automatic weather stations in northwest Greenland, 2012–2020

In situ meteorological data are essential to better understand ongoing environmental changes in the Arctic. Here, we present a dataset of quality-controlled meteorological observations from two automatic weather stations in northwest Greenland from July 2012 to the end of August 2020. The stations were installed in the accumulation area on the Greenland Ice Sheet (SIGMA-A site, 1490 m a.s.l.) and near the equilibrium line of the Qaanaaq Ice Cap (SIGMA-B site, 944 m a.s.l.). We describe the two-step sequence of quality-controlling procedures that we used to create increasingly reliable datasets by masking erroneous data records. Those datasets are archived in the Arctic Data archive System (ADS) (SIGMA-A – https://doi.org/10.17592/001.2022041303, Nishimura et al., 2023f; SIGMA-B – https://doi.org/10.17592/001.2022041306, Nishimura et al., 2023c). We analyzed the resulting 2012–2020 time series of air temperature, surface height, and surface albedo and histograms of longwave radiation (a proxy of cloudiness). We found that surface height increased, and no significant albedo decline in summer was observed at the SIGMA-A site. In contrast, high air temperatures and frequent clear-sky conditions in the summers of 2015, 2019, and 2020 at the SIGMA-B site caused significant albedo and surface lowering. Therefore, it appears that these weather condition differences led to the apparent surface height decrease at the SIGMA-B site but not at the SIGMA-A site. We anticipate that this quality-controlling method and these datasets will aid in climate studies of northwest Greenland and will contribute to the advancement of broader polar climate studies.</p

Prognostic value of tissue-type plasminogen activator (tPA) and its complex with the type-1 inhibitor (PAI-1) in breast cancer

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Heart Failure-Inducible Gene Therapy Targeting Protein Phosphatase 1 Prevents Progressive Left Ventricular Remodeling

BACKGROUND: The targeting of Ca(2+) cycling has emerged as a potential therapy for the treatment of severe heart failure. These approaches include gene therapy directed at overexpressing sarcoplasmic reticulum (SR) Ca(2+) ATPase, or ablation of phospholamban (PLN) and associated protein phosphatase 1 (PP1) protein complexes. We previously reported that PP1β, one of the PP1 catalytic subunits, predominantly suppresses Ca(2+) uptake in the SR among the three PP1 isoforms, thereby contributing to Ca(2+) downregulation in failing hearts. In the present study, we investigated whether heart-failure-inducible PP1β-inhibition by adeno-associated viral-9 (AAV9) vector mediated gene therapy is beneficial for preventing disease progression in genetic cardiomyopathic mice. METHODS: We created an adeno-associated virus 9 (AAV9) vector encoding PP1β short-hairpin RNA (shRNA) or negative control (NC) shRNA. A heart failure inducible gene expression system was employed using the B-type natriuretic protein (BNP) promoter conjugated to emerald-green fluorescence protein (EmGFP) and the shRNA sequence. AAV9 vectors (AAV9-BNP-EmGFP-PP1βshRNA and AAV9-BNP-EmGFP-NCshRNA) were injected into the tail vein (2×10(11) GC/mouse) of muscle LIM protein deficient mice (MLPKO), followed by serial analysis of echocardiography, hemodynamic measurement, biochemical and histological analysis at 3 months. RESULTS: In the MLPKO mice, BNP promoter activity was shown to be increased by detecting both EmGFP expression and the induced reduction of PP1β by 25% in the myocardium. Inducible PP1βshRNA delivery preferentially ameliorated left ventricular diastolic function and mitigated adverse ventricular remodeling. PLN phosphorylation was significantly augmented in the AAV9-BNP-EmGFP-PP1βshRNA injected hearts compared with the AAV9-BNP-EmGFP-NCshRNA group. Furthermore, BNP production was reduced, and cardiac interstitial fibrosis was abrogated at 3 months. CONCLUSION: Heart failure-inducible molecular targeting of PP1β has potential as a novel therapeutic strategy for heart failure

Bactericidal Action of Photogenerated Singlet Oxygen from Photosensitizers Used in Plaque Disclosing Agents

Photodynamic therapy (PDT) has been suggested as an efficient clinical approach for the treatment of dental plaque in the field of dental care. In PDT, once the photosensitizer is irradiated with light of a specific wavelength, it transfers the excitation energy to molecular oxygen, which gives rise to singlet oxygen., a major causative pathogen of caries, followed by erythrosine and phloxine, both of which showed activity similar to each other. One of the reasons for the discrepancy between the singlet oxygen generating ability and bactericidal activity was the incorporation efficiency of the photosensitizers into the bacterial cells. The incorporation rate of rose bengal was the highest among the three photosensitizers examined in the present study, likely leading to the highest bactericidal activity. Meanwhile, the addition of L-histidine, a singlet oxygen quencher, cancelled the bactericidal activity of any of the three photoactivated photosensitizers, proving that singlet oxygen was responsible for the bactericidal action.It is strongly suggested that rose bengal is a suitable photosensitizer for the plaque disclosing agents as compared to the other two photosensitizers, phloxine and erythrosine, when used for PDT