Source and target enzyme signature in serine protease inhibitor active site sequences

Amino acid sequences of proteinaceous proteinase inhibitors have been extensively analysed for deriving information regarding the molecular evolution and functional relationship of these proteins. These sequences have been grouped into several well defined families. It was found that the phylogeny constructed with the sequences corresponding to the exposed loop responsible for inhibition has several branches that resemble those obtained from comparisons using the entire sequence. The major branches of the unrooted tree corresponded to the families to which the inhibitors belonged. Further branching is related to the enzyme specificity of the inhibitor. Examination of the active site loop sequences of trypsin inhibitors revealed that here are strong preferences for specific amino acids at different positions of the loop. These preferences are inhibitor class specific. Inhibitors active against more than one enzyme occur within a class and confirm to class specific sequence in their loops. Hence, only a few positions in the loop seem to determine the specificity. The ability to inhibit the same enzyme by inhibitors that belong to different classes appears to be a result of convergent evolution

An Application of Analytic Hierarchy Process in Vehicle Routing Problem

Vehicle Routing Problem (VRP) facilitates on finding a set of trips, one for each vehicle and to deliver known quantities of goods from a single depot to a set of geographically dispersed customers. This paper proposes an effective hybrid approach that combines customer prioritization with the Clarke and Wright's savings algorithm to solve the capacitated vehicle routing problem. In this model, in addition to traditional objective of resolving vehicle routing problem, the customer satisfaction have been taken into account. Initially, all the customers have been clustered with the help of Clarke and Wright's saving algorithm and later the customers have been prioritized on assigning optimal route using Analytic Hierarchy Process (AHP) as a Multi Criteria Decision Making (MCDM) tool. The highlight of this research is to diminish the total transportation cost without violating the vehicle capacity and ultimately improve the customer satisfaction

Achieving Efficient Strong Scaling with PETSc using Hybrid MPI/OpenMP Optimisation

The increasing number of processing elements and decreas- ing memory to core ratio in modern high-performance platforms makes efficient strong scaling a key requirement for numerical algorithms. In order to achieve efficient scalability on massively parallel systems scientific software must evolve across the entire stack to exploit the multiple levels of parallelism exposed in modern architectures. In this paper we demonstrate the use of hybrid MPI/OpenMP parallelisation to optimise parallel sparse matrix-vector multiplication in PETSc, a widely used scientific library for the scalable solution of partial differential equations. Using large matrices generated by Fluidity, an open source CFD application code which uses PETSc as its linear solver engine, we evaluate the effect of explicit communication overlap using task-based parallelism and show how to further improve performance by explicitly load balancing threads within MPI processes. We demonstrate a significant speedup over the pure-MPI mode and efficient strong scaling of sparse matrix-vector multiplication on Fujitsu PRIMEHPC FX10 and Cray XE6 systems

Isolation and screening of proteolytic bacteria from freshwater fish Cyprinus carpio

The present study was undertaken to isolate and characterize the protease producing potent bacteria from gastro intestinal tract of the freshwater fish Cyprinus carpio. The protease producer was characterized as Bacillus sp. morphologically and biochemically. The optimization studies for growth and proteases production of the isolate Bacillus sp. was carried out. The optimum temperature for the isolated Bacillus sp. was 350C but the optimum temperature for the protease production was 450C, the optimum pH was 7.0 in which the isolate produced 18 mMol/ml and showed a remarkable number of Colony Forming Unit. Hence, it was focused to study the intestinal Bacillus sp. in freshwater fish and its protease enzyme.&nbsp

Modelización por Cadenas de Markov de la evolución de la deformación en vigas de hormigón armado flexotraccionadas

From the analysis of experimentally observed variations in surface strains with loading in reinforced concrete beams, it is noted that there is a need to consider the evolution of strains (with loading) as a stochastic process. Use of Markov Chains for modeling stochastic evolution of strains with loading in reinforced concrete flexural beams is studied in this paper. A simple, yet practically useful, bi-level homogeneous Gaussian Markov Chain (BLHGMC) model is proposed for determining the state of strain in reinforced concrete beams. The BLHGMC model will be useful for predicting behavior/response of reinforced concrete beams leading to more rational design.A través del análisis de la evolución de la deformación superficial observada experimentalmente en vigas de hormigón armado al entrar en carga, se constata que dicho proceso debe considerarse estocástico. En este trabajo se estudia la utilización de cadenas de Markov para modelizar la evolución estocástica de la deformación de vigas flexotraccionadas. Se propone, para establecer el estado de deformación de estas, un modelo con distribución gaussiana tipo cadena de Markov homogénea de dos niveles (BLHGMC por sus siglas en inglés), cuyo empleo resulta sencillo y práctico. Se comprueba la utilidad del modelo BLHGMC para prever el comportamiento de estos elementos, lo que determina a su vez una mayor racionalidad a la hora de su cálculo y diseñ

Trend analysis and variability of satellite-based soil moisture data for the Lower Bhavani basin, Tamil Nadu using Google Earth Engine

Soil moisture is a significant hydrological component that is dynamic in nature. The variation in soil moisture in the basin scale would affect the vegetation, ecology and environment. Soil moisture trend analysis aids in providing the variation of soil moisture over the basin. The present study aimed to analyse the soil moisture trend in Lower Bhavani basin, Tamil Nadu from 2003-2022. Satellite-based soil moisture Global Land Data Assimilation System (GLDAS) data was extracted from the Google Earth Engine (GEE) platform to analyse the variation and trend over the period of time. The highest and lowest soil moisture was observed during monsoon and summer months and its percentage variation was studied. Using Man-Kendall test and Sen’s slope, trend analysis was calculated for two decades (2003-2012 and 2013-2022). In 2003-2012, an increasing trend of soil moisture was observed during winter (October to February); from 2013-2022, an increasing trend was observed during both winter (October to February) and monsoon seasons (June to September). The remaining season did not follow any trend, and there was no decreasing trend in soil moisture. The trend analysis of the study will help to monitor and manage the environmental system across the Lower Bhavani basin

Secure Transmission by Using Mobile Ad hoc Networks

The use of communication security protocols originally developed for wire line and WiFi networks can also place a heavy burden on the limited network resources of a MANET. The framework is designed to allow existing network and routing protocols to perform their functions, whilst providing node authentication, access control, and communication security mechanisms.Simulation results comparing SUPERMAN with IPsec, SAODV and SOLSR are provided to demonstrate the proposed frameworks suitability for wireless communication security. It provide secure transmission of data at the time the hackers hit the process received only empty data. This paper presents a novel security framework for MANETs, SUPERMAN. To overcome the traffic issues, delay of transmission nodes and protect from attackers.The data transmission in secure path

Generation of large flavor mixing from radiative corrections

We provide a model independent criterion which would guarantee a large flavor mixing of two quasi-degenerate Majorana neutrinos at the low scale, irrespective of the mixing at the high scale. We also show that such a situation is realizable for a phenomenologically interesting range of parameters of the weak scale theory. We further show that for a similar condition to be implementable for the three generation case, the CP parity of one of the neutrinos needs to be opposite to that of the others.Comment: 14 pages RevTeX, 2 eps figures. Minor changes made, a few references adde

Online Polling and Feedback System

Online polling and feedback system enable users to cast their vote and opinion in a secure and private way which is intuitive.It is a system in which Voter can cast their votes from anywhere in the country without visiting to voting booths, in a highly secured way which makes voting a valiant of violence and that increases the percentage of voting. The main goal of voting is to come up with leaders of people's choice. It needs to be secretive, anonymous, fast, and reliable. It also creates and manages the voting and an election detail as the user login by email id, voter id and security key as details and click on his favorable candidate to cast his/her vote. This will increase the voting percentage in India. By applying high security, false votes will be reduced. The proposed software is developed and tested to work on Ethernet and allows online voting. It provides improved features of voting system over traditional voting system such as accuracy, handiness, flexibility, privacy, verifiability and mobility

Large Neutrino Mixing from Renormalization Group Evolution

The renormalization group evolution equation for two neutrino mixing is known to exhibit nontrivial fixed point structure corresponding to maximal mixing at the weak scale. The presence of the fixed point provides a natural explanation of the observed maximal mixing of $\nu_{\mu}-\nu_{\tau}$ if the $\nu_{\mu}$ and $\nu_{\tau}$ are assumed to be quasi-degenerate at the seesaw scale without constraining on the mixing angles at that scale. In particular, it allows them to be similar to the quark mixings as in generic grand unified theories. We discuss implementation of this program in the case of MSSM and find that the predicted mixing remains stable and close to its maximal value, for all energies below the $O$(TeV) SUSY scale. We also discuss how a particular realization of this idea can be tested in neutrinoless double beta decay experiments.Comment: Latex file, 21 pages and 4 ps figures include