Polarization-modulation near-field optical microscope for quantitative local dichroism mapping

A couple of experimental techniques have been implemented to an aperture near-field scanning optical microscopy (NSOM) to obtain reliable measurement of sample dichroism on the local scale. First, a method to test NSOM tapered fiber probes toward polarization conservation into the near optical field is reported. The probes are characterized in terms of the in-plane polarization of the near field emerging from their aperture, by using a thin dichroic layer of chromophore molecules, structured along stretched polymeric chains, to probe such polarization when approached in the near-field region of the probe. Second, to assure that the light intensity coupled in the fiber is polarization independent, an active system operating in real time has been realized. Such combination of techniques allowed quantitative imaging of local dichroism degree and average orientation by means of dual-phase lock-in demodulation of the optical signal. Translation of the coupled light polarization state in the near field has been observed for one-half of the tested probes. For the others, the tip acts as a polarizer, and therefore showed it was not suitable for polarization modulation NSOM measurements

A study of the deep structure of the energy landscape of glassy polystyrene: the exponential distribution of the energy-barriers revealed by high-field Electron Spin Resonance spectroscopy

The reorientation of one small paramagnetic molecule (spin probe) in glassy polystyrene (PS) is studied by high-field Electron Spin Resonance spectroscopy at two different Larmor frequencies (190 and 285 GHz). The exponential distribution of the energy-barriers for the rotational motion of the spin probe is unambigously evidenced at both 240K and 270K. The same shape for the distribution of the energy-barriers of PS was evidenced by the master curves provided by previous mechanical and light scattering studies. The breadth of the energy-barriers distribution of the spin probe is in the range of the estimates of the breadth of the PS energy-barriers distribution. The evidence that the deep structure of the energy landscape of PS exhibits the exponential shape of the energy-barriers distribution agrees with results from extreme-value statistics and the trap model by Bouchaud and coworkers.Comment: Final version in press as Letter to the Editor on J.Phys.:Condensed Matter. Changes in bol

Glassforming Liquids, Amorphous and Semicrystalline Polymers: Exploring their Energy Landscape and Dynamical Heterogeneity by Multi-frequency High-Field EPR

We review past and recent work carried out on viscous liquids, amorphous and semicrystalline polymers by multifrequency high-field electron paramagnetic resonance (HF-EPR) facility in Pisa. The emphasis is on the enhanced ability to provide fine details of the reorientation process of the paramagnetic guest, the spin probe, revealing features driving the dynamics of the host system, including the energy-barrier distribution of glassy polymers, the dynamical heterogeneity of semicrystalline polymers, and the dynamical changes occurring at the critical temperature predicted by the ideal mode-coupling theory

High-field Electron Spin Resonance of Cu_{1-x}Zn_{x}GeO_{3}

High-Field Electron Spin Resonance measurements were made on powder samples of Cu_{1-x}Zn_{x}GeO_{3} (x=0.00, 0.01, 0.02, 0.03 and 0.05) at different frequencies (95, 110, 190, 220, 330 and 440 GHz) at low temperatures. The spectra of the doped samples show resonances whose positions are dependent on Zn concentration, frequency and temperature. The analysis of intensity variation of these lines with temperature allows us to identify them as originating in transitions within states situated inside the Spin Peierls gap. A qualitative explanation of the details of the spectra is possible if we assume that these states in the gap are associated with "loose" spins created near the Zn impurities, as recently theoreticaly predicted. A new phenomenon of quenching of the ESR signal across the Dimerized to Incommensurate phase-boundary is observed.Comment: 4 pages, 5 ps figures in the text, submitted to Phys. Rev. Let

Forest nurseries and the National Recovery and Resilience Plan: the case of Sicily and Apulia (Italy)

In Italy, the National Recovery and Resilience Plan (NRRP) foresees the planting of ca. 6.6 million trees to establish urban and peri-urban forests in 14 metropolitan cities. This ambitious project requires a significant number of native trees and shrubs, currently unavailable in Italian public and private nurseries. This survey analyzes the state of forest nurseries in two administrative regions of southern Italy, i.e. the “Filici” forest nursery (province of Agrigento, Sicily) and the Gargano Mountain Reclamation Consortium (province of Foggia, Apulia), to evaluate the adequacy of Sicilian and Apulian forest nurseries as potential sources of plant material to meet the requirements of the NRRP. The census carried out at the “Filici” nursery revealed the presence of more than 22,000 seedlings in cultivation, comprising 55 species, 26 genera, and 26 families. The autochthonous species are 43 (78.2%), while the exotic ones are 12 (21.8%). The Gargano Mountain Reclamation Consortium forest nursery has 190,876 seedlings in cultivation. A total of 80 species are present, belonging to 59 genera and 32 families. Of these, 68 (85%) are native species, 12 (15%) are exotic. An analysis of the plant material being cultivated in these forest nurseries shows the presence of a share of autochthonous species of interest for urban reforestation initiatives, however these are still insufficient in number

Folding and unfolding phylogenetic trees and networks

Phylogenetic networks are rooted, labelled directed acyclic graphs which are commonly used to represent reticulate evolution. There is a close relationship between phylogenetic networks and multi-labelled trees (MUL-trees). Indeed, any phylogenetic network $N$ can be "unfolded" to obtain a MUL-tree $U(N)$ and, conversely, a MUL-tree $T$ can in certain circumstances be "folded" to obtain a phylogenetic network $F(T)$ that exhibits $T$. In this paper, we study properties of the operations $U$ and $F$ in more detail. In particular, we introduce the class of stable networks, phylogenetic networks $N$ for which $F(U(N))$ is isomorphic to $N$, characterise such networks, and show that they are related to the well-known class of tree-sibling networks.We also explore how the concept of displaying a tree in a network $N$ can be related to displaying the tree in the MUL-tree $U(N)$. To do this, we develop a phylogenetic analogue of graph fibrations. This allows us to view $U(N)$ as the analogue of the universal cover of a digraph, and to establish a close connection between displaying trees in $U(N)$ and reconcilingphylogenetic trees with networks

Different somatic alterations of the HRPT2 gene in a patient with recurrent sporadic primary hyperparathyroidism carrying an HRPT2 germline mutation

Early onset of primary hyperparathyroidism (PHPT) and multiglandular involvement suggest a familial form in which germline mutation of a PHPT-related gene(s) and a somatic event at the same locus can be often demonstrated. We investigated the involvement of multiple endocrine neoplasia type 1 (MEN1) and HRPT2 genes in a 39-year-old man with recurrent PHPT. PHPT was firstly diagnosed at the age of 21 and the patient had two recurrences separated by extended periods of normocalcemia. This unusual history prompted us to investigate other family members and study the MEN1 and HRPT2 genes. An HRPT2 germline missense mutation in exon 3 (R91P) was found in the index case, which was associated with different HRPT2 somatic alterations in each of the three examined parathyroid tumors. These findings are consistent with Knudson's 'two hit' concept of biallelic inactivation of classical tumor suppressor genes. Screening of 15 asymptomatic relatives was negative for the R91P germline mutation. All the three abnormal parathyroid specimens showed cystic features at histology and were negative for parafibromin immunostaining. In one specimen, diffuse parafibromin staining was evident in a rim of normal parathyroid tissue surrounding the adenomatous lesion. Our study shows that different somatic genetic events at the HRPT2 locus are responsible for the asynchronous occurrence of multiple adenomas in a patient carrying an HRPT2 germline mutation. The finding of diffuse parafibromin staining in a rim of normal parathyroid tissue, but not in the contiguous adenomatous lesion, reinforces the concept that loss of parafibromin expression is responsible for the development of parathyroid tumors in this setting

Chemical variability of artificial stone powders in relation to their health effects

The occurrence of highly severe silica-related diseases among the resin- and silica-based artificial stone workers was claimed, associated to an extremely short latency. High levels of exposure and intrinsic properties of AS are thought to modulate the development of silicosis and auto-immune diseases. This study compares parent materials and processed dusts, to shed light on changes of AS occurring in the manufacturing process, through an XRF, EPR and XAS investigation. We point out the extremely wide variability of the materials, the occurrence of chemical signatures impressed by the processing techniques, and the unprecedented generation of stable radicals associated to the lysis of the Si-O chemical bond inside the resin coated respirable crystalline silica. These results suggest that the AS processing in industrial stone workshops can create respirable dusts with peculiar physical and chemical properties, to be correlated to the observed clinical evidences

JAB1 deletion in oligodendrocytes causes senescence-induced inflammation and neurodegeneration in mice

Oligodendrocytes are the primary target of demyelinating disorders, and progressive neurodegenerative changes may evolve in the CNS. DNA damage and oxidative stress are considered key pathogenic events, but the underlying molecular mechanisms remain unclear. Moreover, animal models do not fully recapitulate human diseases, complicating the path to effective treatments. Here we report that mice with cell-autonomous deletion of the nuclear COP9 signalosome component CSN5 (JAB1) in oligodendrocytes develop DNA damage and defective DNA repair in myelinating glial cells. Interestingly, oligodendrocytes lacking JAB1 expression underwent a senescence-like phenotype that fostered chronic inflammation and oxidative stress. These mutants developed progressive CNS demyelination, microglia inflammation, and neurodegeneration, with severe motor deficits and premature death. Notably, blocking microglia inflammation did not prevent neurodegeneration, whereas the deletion of p21CIP1 but not p16INK4a pathway ameliorated the disease. We suggest that senescence is key to sustaining neurodegeneration in demyelinating disorders and may be considered a potential therapeutic target