Molecular characterization of Epstein-Barr virus associated with classical Hodgkin's lymphoma in Tunisia: prevalence of the LMP1 oncegene deletions and A and B viruses strains

Epstein-Barr virus (EBV) is detected in Hodgkin's lymphoma (HL) at variable frequencies, depending on various factors including the geographic location. The prevalence of EBV is high among HL diagnosed in the region of the center of Tunisia. The aim of the current work was to define the genotypic features of EBV in a series of EBV-positive HL in this area, in comparison to those of EBV found in association with benign lymphadenopathies. A 30 bp and a 69 bp deletion within the LMP1 gene were detected in 41% and 11% of HL cases, respectively. In reactive lymph nodes, the 30 bp deletion was found in 43% of the cases. Type A EBV was detected in 84% of HL cases and in 96% of reactive lymphoadenopathies, and type B EBV was detected in others. No significant association was found between histological type of HL, age of the patients, or stage at diagnosis, and the molecular configuration of LMP1. However, LMP1 deletions were found more frequently among male patients. The significance of these results is discussed.Peer reviewe

Epstein-Barr virus in Hodgkin's disease: the example of central Tunisia

The purpose of this study was to evaluate the prevalence of Epstein-Barr virus in Hodgkin disease in Tunisia through a series of 77 cases. Association with Epstein-Barr virus was demonstrated by Epstein-Barr encoded early RNA transcripts (EBER) in situ hybridization in 70 % of cases and by latent membrane protein I (LMPI) immunohistochemistry in 58.4 % of cases. EBER positive cases were more frequent in extreme age clashes ( 54 years) there was no correlation with sex, histologic sub-type and clinical stage. Our findings show a high prevalence for EBV infection in Tunisian Hodgkin's disease particularly among extreme ages

Transferprozesse in der Journalismusforschung : Chancen und Herausforderungen im inter- und transdisziplinären Kontext der Journalismusforschung

Der vorliegende Beitrag setzt sich mit Herausforderungen und Perspektiven von Transfer(prozessen) in der Journalismusforschung auseinander. Transfer wird darin als Handlungsebene definiert, die eine Brücke zwischen Wissenschaft und Praxis ermöglicht. Zur genaueren Systematisierung erfolgt diese Einordnung im Kontext von Interdisziplinarität, Transdisziplinarität und transformativer Wissenschaft. Basierend auf bisherigen Empfehlungen und Reflexionen aus dem Fach sowie eigenen Forschungserfahrungen beschreiben wir Chancen und Herausforderungen, aus denen sich Leitfragen für die Phasen von Transferprozessen entwickeln lassen. Gleichzeitig werden Grenzen und Schwächen solcher Prozesse deutlich. Klar ist: Nicht jede Form der Journalismusforschung braucht Transfer, und das Transferpotenzial ist begrenzt. Die Debatte darüber zeigt aber, dass die Themen Transfer, Interaktion und Kooperation im inter- und transdisziplinären Kontext der Journalismusforschung eine Vielzahl an vielversprechenden Perspektiven und Diskursmöglichkeiten für unser Fach darstellen. Wir sehen in der konstruktiven Diskussion darüber einen wichtigen Impuls für eine sich verändernde Wissenschaft sowie neue Rollenbilder und Herangehensweisen

Structural study, spectroscopic characterization, thermal behavior, DFT calculations and antimicrobial properties of a new hybrid compound, (C7H9N2)2[HgCl4]·H2O

A new bis(benzamidinium)tetrachloridomercurate(II) monohydrate, (C7H9N2)2[HgCl4]·H2O, was synthesized by slow evaporation at room temperature and characterized by powder XRD, IR, Raman, UV-vis spectroscopy and thermal analysis. Single-crystal X-ray diffraction analysis indicates that the asymmetric unit of the title compound is formed by tetrachloridomercurate [HgCl4]2− anion, a protonated organic cation (C7H9N2)2+ and one free water crystallization molecule. The cohesion of the structure is insured by N–H···Cl, O–H···Cl and N–H ···O hydrogen bonds and π-π stacking interactions. The density functional theory (DFT)-optimized structure at the B3LYP/6-311++G(2d,2p) level is compared with the experimentally determined molecular structure. Comprehensive experimental and theoretical structural studies on the compound are carried out through IR and UV-vis spectroscopies. The compound was characterized by thermal analysis to determine its thermal behavior with respect to the temperature. The in vitro antibacterial activity of the compound against different bacterial strains was evaluated. The three-dimensional Hirshfeld surface (3 D-HS) and the two-dimensional fingerprint plots (2 D-FP) reveal that the structure is dominated by H···Cl/Cl···H (51.0%) and H···H (32.3%) contacts.Financial support from the Ministry of Higher Education, Scientific Research and Technology of Tunisia, Spanish Ministerio de Economía y Competitividad (MAT2016-78155-C2-1-R) and Gobierno del Principado de Asturias (GRUPIN-IDI/2018/170) is gratefully acknowledged.Peer reviewe