Induction of tetraploidy in rainbow trout (Oncorhynchus mykiss) by heat shock

This study has been conducted for determination of optimum time and duration of thermal shock on tetraploidy induction in Rainbow trout. This study was carried out by induction of 28°C thermal shock in different times after fertilization (49.5, 54.0, 58.5, 63.0, 67.5, 72.0, 76.5 and 81,0°C/hour and different duration (8, 10 and 12 min). Nuclear erythrocytes, cellular area and volume measurement were determined among blood smears of fingerling and statistic analysis showed that, induction of tetraploidy was conducted between 0-75% on various treatment, but highest tetraploidy yield (8.4%) was achieved by thermal shock on 28°C within 12 minutes, 74 degree-hour after fertilization. Moreover, tetra ploidy diagnostic was also approved by determination of nucleolus number in fishes. In this respect tetraploid and diploid fishes have 3-4 and 1-2 nucleolus, respectively

An investigation on the micropyle number in the ova of the strugeon species in Caspian Sea

The micropyle number in the ova of sturgeon species from the South Caspian Sea was investigated. The study was conducted on female broodfishes of three species of sturgeon (Persian sturgeon Acipenser persicus, Stetates sturgeon A. stellatus and great sturgeon Huso huso) included 44 Persian sturgeon, 13 stellate sturgeon and 8 great sturgeon specimens. Fifty eggs were randomly collected from each broodfish and the micropyle number of totally 3250 eggs was determined. Out of 44 Persian sturgeon used, 14 specimens were collected from the south-east Caspian region (Golestan Province, Shaid Marjani Center) and 30 were from the south-west Caspian region (Guilan Province, Shahid Dr. Beheshti Center). The stellate sturgeon specimens were taken from the south-west and the great sturgeon specimens from the south-east Caspian region. The obtained result showed that the mean micropyle number in Persian sturgeon caught from the south-east Caspian region was 8 whereas in those caught from the south-west region was 9. There was no significant difference (p>0.95) in the micropyle number of Persian sturgeon collected from the south-east and south-west regions. The mean micropyle number determined for stellate and great sturgeon was 5. The outer diameter of the micropyle in the Persian, stellate, and great sturgeon were 22 μm, 17 μm and 19 μm respectively

The possibility of sex identification in beluga (Huso huso) by using PCR-RAPD technique

The goal of this study was to find a sex-specific DNA sequences in the male and female genomes of beluga (Huso huso), by random amplified polymorphic DNA (RAPD) assays performed on pooled DNA samples. Of the 310 different primers was tested, 7 primers produced no band. The most likely possibility is that PCR conditions have not been suitable for these 7 primers. It is also possible that these RAPD primers did not find suitable binding position to generate fragments. A single band was produced by primer 295 (OPT -17) which was specific to the male DNA. Further analysis of individual male and female beluga demonstrated that this primer yielded a product that were in fact variable and reproducible, but were the result of variation in some individual males and females and not male-specific. Therefore, of the 4146 RAPDs scored, none were tightly linked to a sex-determining gene. The results support the idea that sex chromosomes are either not present or they weakly differentiated in the genome of beluga

Comparison of digestive enzyme activity in the stomach, pyloric caeca and intestine in diploid and triploid female of rainbow trout (Oncorhynchus mykiss)

The effects of chromosome manipulation on the digestive enzyme activity in the rainbow trout were studied. The enzymes included Pepsin, Trypsin, Chymotrypsin, cileimylase, Lipase and Alkaline Phosphatase which were assessed in diploid and triploid female of rainbow trout. Pepsin activity in the stomach of the assessed fish showed no significant difference between the diploid and triploid fish (P>0.05). The measurement of Trypsin and Chymotrypsin activity in the intestine and pyloric caeca revealed no significant difference in the treated and untreated fish (P>0.05). The activity of a-Amylase, Lipase and Alkaline Phosphatase showed no significant difference in the intestine and pyloric caeca of the diploid and triploid fish (P>0.05).The results indicated that chromosome manipulation in rainbow trout had no effects on digestive enzyme activity

Clinicopathologic Characteristics of Multifocal Gastric Adenocarcinoma

BACKGROUNDANDOBJECTIVE: Gastric cancer is the fifth prevalent cancer in worldwide. Among of this, adenocarcinoma is more than 95% of all case and most prevalent. According to gastric adenocarcinoma is resistant to adjuvant therapy, surgical resection is the cardinal strategies of treatment. One of the problems with treating adenocarcinoma of the stomach is that its main causes are malignant or pre-malignant changes in the residual gastric tissue that may be neglected during resection. By recognizing these lesions and resection, they can be expected to reduce the malignancy. Therefore, this study was conducted to investigate the characteristics of non-tumoral gastric tissue in terms of malignant or pre-malignant changes. METHODS: In this retrospective cross-sectional study, 112 patients who had undergone total gastrectomy in the hospitals affiliated to Babol University of Medical Sciences during the period of 2013-2017 were examined and analyzed for age, sex, type and location of tumor. FINDINGS: Of 112 cases that studied,72 cases (64.3%) were male and 40 cases (35.7%) were female. The mean of ages was 64.7±8.6 year. Poorly differentiated pathology in 60.7% and well differentiated pathology in 39.3% of patients were seen. In 64 patients (57%), there was synchronous lesions in addition to primary pathology. These synchronous lesions were related statistically significant to pathology, cancer stage and gender. CONCLUSION: Based on the results of this study, it can be concluded that resection should be accompanied by considering simultaneous lesions in addition to primary pathology, which can be a reason for total gastrectomy as a standard treatment for gastric cancer

Harmonizing semantic annotations for computational models in biology

Life science researchers use computational models to articulate and test hypotheses about the behavior of biological systems. Semantic annotation is a critical component for enhancing the interoperability and reusability of such models as well as for the integration of the data needed for model parameterization and validation. Encoded as machine-readable links to knowledge resource terms, semantic annotations describe the computational or biological meaning of what models and data represent. These annotations help researchers find and repurpose models, accelerate model composition and enable knowledge integration across model repositories and experimental data stores. However, realizing the potential benefits of semantic annotation requires the development of model annotation standards that adhere to a community-based annotation protocol.Without such standards, tool developers must account for a variety of annotation formats and approaches, a situation that can become prohibitively cumbersome and which can defeat the purpose of linking model elements to controlled knowledge resource terms. Currently, no consensus protocol for semantic annotation exists among the larger biological modeling community. Here, we report on the landscape of current annotation practices among the Computational Modeling in BIology NEtwork community and provide a set of recommendations for building a consensus approach to semantic annotation

Extraction of uranium with TBP in an electrostatic apparatus

SIGLEAvailable from British Library Document Supply Centre- DSC:D37903/81 / BLDSC - British Library Document Supply CentreGBUnited Kingdo

A fine-grained pipelined 2-D convolver for high-performance applications

2-D convolution has been used as a subsystem for filtering and enhancement in a wide range of signal and image processing applications. It is not only a memory intensive operation but also a compute intensive process. The previous efforts to improve the performance of 2-D convolution have primarily focused on the memory access challenges. However, the convolver's performance is highly dependent on the efficient design of the computation units, which can be enhanced significantly by employing some techniques such as pipelining. In this brief, a pipelined with low pixel access rate architecture for implementation of 2-D convolution is presented. Compared to the conventional convolvers, the proposed design involves a fixed (independent to the problem/kernel) size, and a significantly shorter critical path specifically for large kernel sizes where the proposed convolver works with 283-MHz clock frequency, on a Xilinx Virtex-7 (XC7V2000t) field-programmable gate array, for a 3 × 3 kernel. Additionally, the required pixel access rate of the new scheme is less than that of the state-of-the-art methods, which is only 849 Mb/s for a 3 × 3 kernel and 8-bit pixels. The improvements in the critical path delay and the required pixel access rate are obtained without significant increase in the resource utilization.Mahdi Kalbasi, Hooman Nikmeh