Application of chicken microarrays for gene expression analysis in other avian species

BACKGROUND: With the threat of emerging infectious diseases such as avian influenza, whose natural hosts are thought to be a variety of wild water birds including duck, we are armed with very few genomic resources to investigate large scale immunological gene expression studies in avian species. Multiple options exist for conducting large gene expression studies in chickens and in this study we explore the feasibility of using one of these tools to investigate gene expression in other avian species. RESULTS: In this study we utilised a whole genome long oligonucleotide chicken microarray to assess the utility of cross species hybridisation (CSH). We successfully hybridised a number of different avian species to this array, obtaining reliable signals. We were able to distinguish ducks that were infected with avian influenza from uninfected ducks using this microarray platform. In addition, we were able to detect known chicken immunological genes in all of the hybridised avian species. CONCLUSION: Cross species hybridisation using long oligonucleotide microarrays is a powerful tool to study the immune response in avian species with little available genomic information. The present study validated the use of the whole genome long oligonucleotide chicken microarray to investigate gene expression in a range of avian species

Sustainable organic plant breeding: Final report - a vision, choices, consequences and steps

In general, the characteristics of organic varieties - and by extension of organic plant breeding - differ from that of conventional breeding systems and conventional varieties. Realising an organic plant breeding system and subsequently steering it to meet changing demands is no less than a mammoth task. The many actions to be undertaken can be divided into short-term commercial and scientific activities, and longer or long-term commercial and scientific activities. Action must be taken in the short-term to ensure adequate quantities of organically propagated plants and seed. This is vital in consideration of Regulation 2092/91/EC which states that, as of 1 January 2000, all propagating material used in organic production must be of organic origin. Additional measures are needed to accelerate the development of organically propagated varieties. Within the breeding sector, variety groups should be established to streamline communication in the chain. Variety groups should have a large contingent of farmers, as well as representatives from the trade branch and breeders. Members should communicate intensively with each other, share experiences, and participate in trials and variety assessments. Questions, wishes and bottlenecks could be recorded by variety groups and passed on to other parties in the chain. The practical details of the plant health concept which is at the basis of organic breeding must be worked out (operationalised). This will require scientific research, for example on: root development and mineral absorption efficiency weed suppressive capacity in situ versus ex situ maintenance resistance breeding in combination with cultivation measures seed-transmitted diseases adaptive capacity alternatives for growth stimulants, silver nitrate and silver thiosulfate in the cultivation of cucumbers and pickles Such research should be carried out by academic institutions (such as Wageningen University and Research Centre) in collaboration with Louis Bolk Institute, Stichting Zaadgoed and private companies. A platform should be established to make an inventory of problems and priorities and to develop research proposals. Farmers could contribute their ideas to the platform through the variety groups. Conclusion A plant breeding system for organic production should be based on the organic concept of plant health and on the organic position on chain relationships. As the total land area under organic production is still relatively small, it is unlikely that commercial breeders will make large investments to develop organic breeding programmes without financial support from other parties, i.e. the government. In this early stage, it is vital that the government provides generous funding and plays an active enabling role. We hope that the action plan to stimulate organic plant breeding, as requested by Parliament, will dovetail with the activities described above

Time-resolved broadband analysis of slow-light propagation and superluminal transmission of electromagnetic waves in three-dimensional photonic crystals

A time-resolved analysis of the amplitude and phase of THz pulses propagating through three-dimensional photonic crystals is presented. Single-cycle pulses of THz radiation allow measurements over a wide frequency range, spanning more than an octave below, at and above the bandgap of strongly dispersive photonic crystals. Transmission data provide evidence for slow group velocities at the photonic band edges and for superluminal transmission at frequencies in the gap. Our experimental results are in good agreement with finite-difference-time-domain simulations.Comment: 7 pages, 11 figure

First Observation of 15Be

The neutron-unbound nucleus 15Be was observed for the first time. It was populated using neutron transfer from a deuterated polyethylene target with a 59 MeV/u 14Be beam. Neutrons were measured in coincidence with outgoing 14Be particles and the reconstructed decay energy spectrum exhibits a resonance at 1.8(1) MeV. This corresponds to 15Be being unbound by 0.45 MeV more then 16Be thus significantly hindering the sequential two-neutron decay of 16Be to 14Be through this state

Healthy Dietary Interventions and Lipoprotein (a) Plasma Levels: Results from the Omni Heart Trial

Background: Increased lipoprotein(a) [Lp(a)] levels are associated with atherosclerotic cardiovascular disease. Studies of dietary interventions on changes in Lp(a) are sparse. We aimed to compare the effects of three healthy dietary interventions differing in macronutrient content on Lp(a) concentration. Methods: Secondary analysis of a randomized, 3-period crossover feeding study including 155 (89 blacks; 66 whites) individuals. Participants were given DASH-type healthy diets rich in carbohydrates [Carb], in protein [Prot] or in unsaturated fat [Unsat Fat] for 6 weeks each. Plasma Lp(a) concentration was assessed at baseline and after each diet. Results: Compared to baseline, all interventional diets increased mean Lp(a) by 2 to 5 mg/dl. Unsat Fat increased Lp(a) less than Prot with a difference of 1.0 mg/dl (95% CI, −0.5, 2.5; p = 0.196) in whites and 3.7 mg/dl (95% CI, 2.4, 5.0; p<0.001) in blacks (p-value between races = 0.008); Unsat Fat increased Lp(a) less than Carb with a difference of −0.6 mg/dl, 95% CI, −2.1, 0.9; p = 0.441) in whites and −1.5 mg/dl (95% CI, −0.2, −2.8; p = 0.021) in blacks (p-value between races = 0.354). Prot increased Lp(a) more than Carb with a difference of 0.4 mg/dl (95% CI, −1.1, 1.9; p = 0.597) in whites and 2.2 mg/dl (95%CI, 0.9, 3.5; p = 0.001) in blacks (p-value between races = 0.082). Conclusion: Diets high in unsaturated fat increased Lp(a) levels less than diets rich in carbohydrate or protein with greater changes in blacks than whites. Our results suggest that substitutions with dietary mono- and polyunsaturated fatty acids in healthy diets may be preferable over protein or carbohydrates with regards to Lp(a). Trial Registration Clinicaltrials.gov NCT0005135

Further Insights into the Reaction Be14(CH2,X)10He

A previously published measurement of the reaction of a 59 MeV/nucleon 14Be beam on a deuterated polyethylene target was further analyzed to search for 12He as well as initial state effects in the population of the 10He ground state. No evidence for either was found. A lower limit of about 1 MeV was determined for a possible resonance in 12He. In addition, the three-body decay energy spectrum of 10He could not be described by a reaction mechanism calculation based on the halo structure of the initial 14Be assuming a direct α-particle removal reaction

Structure and Decay Correlations of Two-Neutron Systems Beyond the Dripline

The two-neutron unbound systems of 16Be, 13Li, 10He, and 26O have been measured using the Modular Neutron Array (MoNA) and 4 Tm Sweeper magnet setup. The correlations of the 3-body decay for the 16Be and 13Li were extracted and demonstrated a strong correlated enhancement between the two neutrons. The measurement of the 10He ground state resonance from a 14Be(−2p2n) reaction provided insight into previous predictions that wavefunction of the entrance channel, projectile, can influence the observed decay energy spectrum for the unbound system. Lastly, the decay-in-target (DiT) technique was utilized to extract the lifetime of the 26O ground state. The measured lifetime of 4.5+1.1 −1.5 (stat.)±3(sys.) ps provides the first indication of two-neutron radioactivity