Richness and Abundance of Carabidae and Staphylinidae (Coleoptera), in Northeastern Dairy Pastures Under Intensive Grazing

Dairy cattle has become popular to dairy farmers in the Northeast looking for management schemes to cut production costs. Carabidae (ground beetles) and Staphylinidae (rove beetles) are indicators of habitat disturbances, such as drainage of wetlands, or grassland for grazing animals, and their monitoring could provide one measure of ecosystem sustainability if intensive management systems expand or intensify in the future. Our objective was assess the abundance and species richness of these two beetle families under intensive grazing throughout Pennsylvania, southern New York and Vermont. We collected 4365 ground beetles (83 species) and 4,027 rove beetles (79 species) by pitfall traps in three years in Pennsylvania. Nine ground beetle species, Amara aenea, Poecilus chalcites, Pterostichus melanarius, Bembidion quadrimaculatum oppositum, Amara familiaris, Poecilus lucublandus, Agonum muelleri, Bembidion obtusum and Bembidion mimus represented 80% of the Carabidae collected. Five other species were new to Pennsylvania. Four rove beetle species, Philonthus cognatus, Meronera venustula, Amischa analis, and Philonthus various = (carbonarius), comprised 74% of the total Staphylinidae collected. Yearly distributions of the dominant species did not change significantly in the three years with A. aenea and P. cognatus being most abundant every year. A parasitic rove beetle, Aleochara tristis, was recovered for the first time in Pennsylvania and Vermont since its release in the 1960\u27s to control face fly, Musca autumnalis. Similar results were found in New York and Vermont. We collected 1,984 ground beetles (68 species). Pterostichus melanarius was most abundant. Pterostichus vernalis was detected for the first time in the United States (Vermont). It was previously reported from Montreal, Canada. We collected 843 rove beetles (45 species). Philonthus cognatus was the most abundant rove beetle. In addition, Tachinus corticinus, previously known only from Canada, was discovered for the first time in the United States in Vermont. Pastures in Pennsylvania were diverse, containing 14 species of forage plants and 17 weed species. Botanical composition was similar in New York and Vermont. Sixteen species of grasses and legumes made up 90% of the plant composition and 36 species of weeds made up the remainder. This di­verse plant ecosystem may explain the richness of ground and rove beetles in northeastern U.S. pastures because the heterogeneity in the plant population provided additional resources which can support a rich assemblage of beetles. Monitoring richness and abundance of Carabidae and Staphylinidae over three years in Pennsylvania suggests intensive grazing systems are eco­logically sustainable

AGMIAL: implementing an annotation strategy for prokaryote genomes as a distributed system

We have implemented a genome annotation system for prokaryotes called AGMIAL. Our approach embodies a number of key principles. First, expert manual annotators are seen as a critical component of the overall system; user interfaces were cyclically refined to satisfy their needs. Second, the overall process should be orchestrated in terms of a global annotation strategy; this facilitates coordination between a team of annotators and automatic data analysis. Third, the annotation strategy should allow progressive and incremental annotation from a time when only a few draft contigs are available, to when a final finished assembly is produced. The overall architecture employed is modular and extensible, being based on the W3 standard Web services framework. Specialized modules interact with two independent core modules that are used to annotate, respectively, genomic and protein sequences. AGMIAL is currently being used by several INRA laboratories to analyze genomes of bacteria relevant to the food-processing industry, and is distributed under an open source license

Effects of sphingosine and sphingosine analogues on the free radical production by stimulated neutrophils: ESR and chemiluminescence studies

Sphingolipids inhibit the activation of the neutrophil (PMN) NADPH oxidase by protein kinase C pathway. By electron spin resonance spectroscopy (ESR) and chemiluminescence (CL), we studied the effects of sphingosine (SPN) and ceramide analogues on phorbol 12-myristate 13-acetate (PMA, 5 × 10-7M) stimulated PMN (6 × 106 cells). By ESR with spin trapping (100 mM DMPO: 5,5-dimethyl-1-pyrroline-Noxide), we showed that SPN (5 to 8 × 10-6M), C2-ceramide (N-acetyl SPN) and C6-ceramide (N-hexanoyl SPN) at the final concentration of 2 × 10-5 and 2 × 10-4M inhibit the production of free radicals by stimulated PMN. The ESR spectrum of stimulated PMN was that of DMPO-superoxide anion spin adduct. Inhibition by 5 × 10-6M SPN was equivalent to that of 30 U/ml SOD. SPN (5 to 8 × 10-6M) has no effect on in vitro systems generating superoxide anion (xanthine 50 mM/xanthine oxidase 110 mU/ml) or hydroxyl radical (Fenton reaction: 88 mM H2O2, 0.01 mM Fe2+ and 0.01 mM EDTA). SPN and N-acetyl SPN also inhibited the CL of PMA stimulated PMN in a dose dependent manner (from 2 × 10-6 to 10-5M), but N-hexanoyl SPN was less active (from 2 × 10-5 to 2 × 10-4M). These effects were compared with those of known PMN inhibitors, superoxide dismutase, catalase and azide. SPN was a better inhibitor compared with these agents. The complete inhibition by SPN of ESR signal and CL of stimulated PMN confirms that this compound or one of its metabolites act at the level of NADPH-oxidase, the key enzyme responsible for production of oxygen-derived free radicals

Richness and Abundance of Carabidae and Staphylinidae (Coleoptera), in Northeastern Dairy Pastures Under Intensive Grazing

Dairy cattle has become popular to dairy farmers in the Northeast looking for management schemes to cut production costs. Carabidae (ground beetles) and Staphylinidae (rove beetles) are indicators of habitat disturbances, such as drainage of wetlands, or grassland for grazing animals, and their monitoring could provide one measure of ecosystem sustainability if intensive management systems expand or intensify in the future. Our objective was assess the abundance and species richness of these two beetle families under intensive grazing throughout Pennsylvania, southern New York and Vermont. We collected 4365 ground beetles (83 species) and 4,027 rove beetles (79 species) by pitfall traps in three years in Pennsylvania. Nine ground beetle species, Amara aenea, Poecilus chalcites, Pterostichus melanarius, Bembidion quadrimaculatum oppositum, Amara familiaris, Poecilus lucublandus, Agonum muelleri, Bembidion obtusum and Bembidion mimus represented 80% of the Carabidae collected. Five other species were new to Pennsylvania. Four rove beetle species, Philonthus cognatus, Meronera venustula, Amischa analis, and Philonthus various = (carbonarius), comprised 74% of the total Staphylinidae collected. Yearly distributions of the dominant species did not change significantly in the three years with A. aenea and P. cognatus being most abundant every year. A parasitic rove beetle, Aleochara tristis, was recovered for the first time in Pennsylvania and Vermont since its release in the 1960\u27s to control face fly, Musca autumnalis. Similar results were found in New York and Vermont. We collected 1,984 ground beetles (68 species). Pterostichus melanarius was most abundant. Pterostichus vernalis was detected for the first time in the United States (Vermont). It was previously reported from Montreal, Canada. We collected 843 rove beetles (45 species). Philonthus cognatus was the most abundant rove beetle. In addition, Tachinus corticinus, previously known only from Canada, was discovered for the first time in the United States in Vermont. Pastures in Pennsylvania were diverse, containing 14 species of forage plants and 17 weed species. Botanical composition was similar in New York and Vermont. Sixteen species of grasses and legumes made up 90% of the plant composition and 36 species of weeds made up the remainder. This di­verse plant ecosystem may explain the richness of ground and rove beetles in northeastern U.S. pastures because the heterogeneity in the plant population provided additional resources which can support a rich assemblage of beetles. Monitoring richness and abundance of Carabidae and Staphylinidae over three years in Pennsylvania suggests intensive grazing systems are eco­logically sustainable

In-band network monitoring technique to support SDN-based wireless networks

Most industrial applications demand determinism in terms of latency, reliability, and throughput. This goes hand in hand with the increased complexity of real-time network programability possibilities. To ensure network performance low-overhead, high-granularity, and timely network verification techniques need to be deployed. The first cornerstone of network verification ability is to enable end-to-end network monitoring, including end devices too. To achieve this, this article shows a novel and low overhead in-band network telemetry and monitoring technique for wireless networks focusing on IEEE 802.11 networks. A design of in-band network telemetry enabled node architecture is proposed and its proof of concept implementation is realized. The PoC realization is used to monitor a real-life SDN-based wireless network, enabling on-the-fly (re)configuration capabilities based on monitoring data. In addition, the proposed monitoring technique is validated in terms of monitoring accuracy, monitoring overhead, and network (re)configuration accuracy. It is shown that the proposed in-band monitoring technique has 6 times lower overhead than other active monitoring techniques on a single-hop link. Besides this, it is demonstrated that (re)configuration decisions taken based on monitored data fulfill targeted application requirements, validating the suitability of the proposed monitoring technique

AGMIAL: implementing an annotation strategy for prokaryote genomes as a distributed system

We have implemented a genome annotation system for prokaryotes called AGMIAL. Our approach embodies a number of key principles. First, expert manual annotators are seen as a critical component of the overall system; user interfaces were cyclically refined to satisfy their needs. Second, the overall process should be orchestrated in terms of a global annotation strategy; this facilitates coordination between a team of annotators and automatic data analysis. Third, the annotation strategy should allow progressive and incremental annotation from a time when only a few draft contigs are available, to when a final finished assembly is produced. The overall architecture employed is modular and extensible, being based on the W3 standard Web services framework. Specialized modules interact with two independent core modules that are used to annotate, respectively, genomic and protein sequences. AGMIAL is currently being used by several INRA laboratories to analyze genomes of bacteria relevant to the food-processing industry, and is distributed under an open source license

Long-term implications and global impact of paediatric surgery

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