Evaluation of 3 rapid influenza diagnostic tests during the 2012-2013 epidemic: influences of subtype and viral load.

&lt;p&gt;This article evaluates the performance of 3 rapid influenza diagnostic tests (RIDTs), in correlation with the influenza subtypes and the viral load. A total of 236 samples were prospectively analyzed with BinaxNOW Influenza A/B, Directigen EZ Flu A and B, and bioNexia Influenza A+B. The results were compared to cell cultures and real-time polymerase chain reaction. Positive samples were further subtyped. Thirty-seven samples were positive for influenza A, and 57, for influenza B. For A(H1N1), the sensitivities were 71.42% for BinaxNOW, 78.57% for Directigen, and 67.85% for bioNexia. Eight samples were positive for A(H3N2), and only the bioNexia test had 1 false-negative result. Lowest sensitivities were observed for influenza B/Yamagata, (56.86% for BinaxNOW and Directigen and 39.21% for bioNexia). The 3 evaluated RIDTs were more efficient at detecting influenza A(H3N2) than for A(H1N1) and B/Yamagata. Highest viral loads in the samples were associated with better rate of detection.&lt;/p&gt;</p

Abolition of Herpes simplex cytopathic effect after treatment with peroxidase generated hypothiocyanite

HSV1 suspensions were pre-incubated in a lactoperoxidase thiocyanate oxidizing system before inoculation in MRC5 fibroblast cultures. This pre-treatment could either delay or even abolish the HSV1 cytopathic potentiality. The effect was found to be time-dependent.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Observation of a cytopathogenic effect on cell lines used for routine viral cultures led to the diagnosis of lymphogranuloma venereum.

OBJECTIVES: This article reports the fortuitous recovery of nine Chlamydia trachomatis serovar L strains in cell cultures (Vero and LLC-MK(2) cell line) designed for viral culture. METHODS: Nine ano-genital swabs were inoculated on confluent Vero, MRC5 and LLC-MK(2) cell cultures. They were collected from HIV-positive patients who were primarily men who have sex with men (MSM) presenting ulcerations that mimicked herpes simplex infections. RESULTS: A cytopathogenic effect was observed on Vero and LLC-MK(2) cells on day 14. The presence of C trachomatis serovar L in the cell lines was confirmed by Real Time-PCR. CONCLUSIONS: C trachomatis serovar L can grow on Vero and LLC-MK(2) cell lines designed for viral cultures. Lymphogranuloma venereum must be considered as a differential diagnosis for herpes-like lesions, particularly in MSM with high-risk behaviours.JOURNAL ARTICLESCOPUS: ar.jinfo:eu-repo/semantics/publishe

Prospective evaluation of Coris Influ-A&amp;B Respi-Strip and of BinaxNOW Influenza A&amp;B assay against viral culture and real-time PCR assay for detection of 2009 pandemic influenza A/H1N1v in Belgian patients33848

PURPOSE: Evaluation of the performance of two rapid (15&#039;) antigen detection tests (RAT), BinaxNOW Influenza A&amp;B and Coris Influ-A&amp;B Respi-Strip for the detection of A(H1N1)v2009. STUDY DESIGN: Between July 2009 and November 2009, 4105 respiratory specimens from patients with influenza-like illness attending seven public hospitals in Brussels were prospectively examined by two immunochromatographic RAT, followed by viral culture and/or specific real-time RT-PCR. RESULTS: Samples consisted predominantly of nasopharyngeal aspirates (NPA-41%), nasopharyngeal (NPS-37%) and throat swabs (TS-14%). The sensitivity and specificity of Coris RAT and BinaxNOW RAT were 36.6% and 99.7%, and 47% and 98.7% respectively compared to culture; and 33.7% and 99.6%; and 46.5% and 98.8% compared to RT-PCR. Significant differences in sensitivity could be observed when splitting up the samples by sample type and patient&#039;s age. NPA gave by far the highest sensitivities: 51.1- 62% for Coris compared to culture and 62.6-78.4% for BinaxNOW. Sensitivities in paediatric NPS varied less between different hospitals (34-41.9%) being still much higher than in adult NPS (11.4-20%). TS resulted in unsatisfactory results: 13% sensitivity in children and 10.5% in adults. CONCLUSIONS: Both RAT showed excellent specificities, but insufficient sensitivities. Consequently, negative results should be confirmed. NPA are clearly superior to NPS orTS, and they stay the sample of choice for viral diagnosis</p

Prospective evaluation of Coris Influ-A&B Respi-Strip and of BinaxNOW Influenza A&B assay against viral culture and real-time PCR assay for detection of 2009 pandemic influenza A/H1N1v in Belgian patients.

Evaluation of the performance of two rapid (15') antigen detection tests (RAT), BinaxNOW Influenza A&B and Coris Influ-A&B Respi-Strip for the detection of A(H1N1)v2009.Evaluation StudiesJournal ArticleMulticenter StudySCOPUS: ar.jinfo:eu-repo/semantics/publishe

Performance evaluation of the Binaxnow influenza a & b kit for detection of h1n1v pandemic influenza in belgian paediatric patients

[abstract 1135]info:eu-repo/semantics/nonPublishe

Prospective evaluation of diagnostic tools for respiratory viruses in children and adults

Aim: To compare the performances of molecular and non-molecular tests to diagnose respiratory viral infections and to evaluate the pros and contras of each technique. Methods: Two hundred ninety-nine respiratory samples were prospectively explored using multiplex molecular techniques (FilmArray Respiratory Panel, Clart Pneumovir), immunological techniques (direct fluorescent assay, lateral flow chromatography) and cell cultures. Findings: Molecular techniques permitted the recovery of up to 50% more respiratory pathogens in comparison to non-molecular methods. FilmArray detected at least 30% more pathogens than Clart Pneumovir which could be explained by the differences in their technical designs. The turnaround time under 2 hours for the FilmArray permitted delivery of results when patients were still in the emergency room.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Contribution of the FilmArray respiratory panel in the management of adult and pediatric patients attending the emergency room during 2015-2016 influenza epidemics: an interventional study.

To evaluate the contribution of a multiplex PCR for respiratory viruses on antibiotic and antiviral prescription, ancillary test prescription, admission and length of stay of patients.SCOPUS: ar.jinfo:eu-repo/semantics/publishe