Biological, physical and morphological factors for the programming of a novel microbial hygromorphic material

The urgency for energy efficient, responsive architectures has propelled smart material development to the forefront of scientific and architectural research. This paper explores biological, physical, and morphological factors influencing the programming of a novel microbial-based smart hybrid material which is responsive to changes in environmental humidity. Hygromorphs respond passively, without energy input, by expanding in high humidity and contracting in low humidity.Bacillus subtilisdevelops environmentally robust, hygromorphic spores which may be harnessed within a bilayer to generate a deflection response with potential for programmability. The bacterial spore-based hygromorph biocomposites (HBCs) were developed and aggregated to enable them to open and close apertures and demonstrate programmable responses to changes in environmental humidity. This study spans many fields including microbiology, materials science, design, fabrication and architectural technology, working at multiple scales from single cells to \u27bench-top\u27 prototype.Exploration of biological factors at cellular and ultracellular levels enabled optimisation of growth and sporulation conditions to biologically preprogramme optimum spore hygromorphic response and yield. Material explorations revealed physical factors influencing biomechanics, preprogramming shape and response complexity through fabrication and inert substrate interactions, to produce a palette of HBCs. Morphological aggregation was designed to harness and scale-up the HBC palette into programmable humidity responsive aperture openings. This culminated in pilot performance testing of a humidity-responsive ventilation panel fabricated with aggregatedBacillusHBCs as a bench-top prototype and suggests potential for this novel biotechnology to be further developed

Atmospheres of digital technology: wireless spectres and ghosts outside the machine

This paper examines the atmospheres of invisible technologies in the built environment. Starting with the, often cited, notion of technologies of disappearance, this paper suggest that, in the absence of physically tangible infrastructure, we understand and frame technologies through myths and historic forms of interpretation. Using the specific example of Wi-Fi networks the paper draws parallels between the operation and interpretation of modern wireless infrastructure and the spiritualism of the nineteenth century. Furthermore, it shows how through creative exploration, using a visualisation device and a photographic method developed by the authors, perceptions of these invisible infrastructures and the spaces they occupy can be played with. The work acts as an extension and critique of the way wireless infrastructure is currently described and perceived and through playful exploration may lead to new types of technology and spatial design

Effect of (in)organic additives on microbially induced calcium carbonate precipitation

\ua9 The Author(s) 2023. Aim: This study aimed to understand the morphological effects of (in)organic additives on microbially induced calcium carbonate precipitation (MICP). Methods and results: MICP was monitored in real time in the presence of (in)organic additives: bovine serum albumin (BSA), biofilm surface layer protein A (BslA), magnesium chloride (MgCl2), and poly-L-lysine. This monitoring was carried out using confocal microscopy to observe the formation of CaCO3 from the point of nucleation, in comparison to conditions without additives. Complementary methodologies, namely scanning electron microscopy, energy-dispersive X-ray spectroscopy and X-ray diffraction, were employed to assess the visual morphology, elemental composition, and crystalline structures of CaCO3, respectively, following the crystals’ formation. The results demonstrated that in the presence of additives, more CaCO3 crystals were produced at 100 min compared to the reaction without additives. The inclusion of BslA resulted in larger crystals than reactions containing other additives, including MgCl2. BSA induced a significant number of crystals from the early stages of the reaction (20 min) but did not have a substantial impact on crystal size compared to conditions without additives. All additives led to a higher content of calcite compared to vaterite after a 24-h reaction, with the exception of MgCl2, which produced a substantial quantity of magnesium calcite. Conclusions: The work demonstrates the effect of several (in)organic additives on MICP and sets the stage for further research to understand additive effects on MICP to achieve controlled CaCO3 precipitation

Microbially induced calcium carbonate precipitation through CO₂ sequestration via an engineered <em>Bacillus subtilis</em>

\ua9 The Author(s) 2024. Background: Microbially induced calcium carbonate precipitation has been extensively researched for geoengineering applications as well as diverse uses within the built environment. Bacteria play a crucial role in producing calcium carbonate minerals, via enzymes including carbonic anhydrase—an enzyme with the capability to hydrolyse CO2, commonly employed in carbon capture systems. This study describes previously uncharacterised carbonic anhydrase enzyme sequences capable of sequestering CO2 and subsequentially generating CaCO3 biominerals and suggests a route to produce carbon negative cementitious materials for the construction industry. Results: Here, Bacillus subtilis was engineered to recombinantly express previously uncharacterised carbonic anhydrase enzymes from Bacillus megaterium and used as a whole cell catalyst allowing this novel bacterium to sequester CO2 and convert it to calcium carbonate. A significant decrease in CO2 was observed from 3800 PPM to 820 PPM upon induction of carbonic anhydrase and minerals recovered from these experiments were identified as calcite and vaterite using X-ray diffraction. Further experiments mixed the use of this enzyme (as a cell free extract) with Sporosarcina pasteurii to increase mineral production whilst maintaining a comparable level of CO2 sequestration. Conclusion: Recombinantly produced carbonic anhydrase successfully sequestered CO2 and converted it into calcium carbonate minerals using an engineered microbial system. Through this approach, a process to manufacture cementitious materials with carbon sequestration ability could be developed

TellEat: sharing experiences on the move

In a context where, due to the proliferation of mobile devices, virtual social environments on the Web are taking up a very concrete role in the way people experience their surroundings, the Future Internet seems to be headed toward a mixture of Social Web, Semantic Web and Augmented Reality. As a part of a larger project that aims at building a social network of both people and things, we designed and developed TellEat, an iPhone-based application that allows users in mobility to share facts concerning people or objects that participate in the social network, and to discover pertinent events that have been told by others. In this paper we discuss both the client application, with the interaction model and interface metaphors that have been designed to make the experience as playful as possible for users, and the server-side services that provide the necessary knowledge and reasoning mechanisms. We also present the results of preliminary tests with users

Architectural User Interfaces: Themes, Trends and Directions in the Evolution of Architectural Design and Human Computer Interaction

This paper reviews the historical and contemporary relationships between architectural design and Human Computer Interaction (hereafter HCI).Through this discussion the paper focuses on the enduring use of architecture as a metaphor in interaction design and the growing recognition that architectural space shapes the territory within which we interact with computational information.The paper begins with a brief discussion of the History of HCI before examining the relationship between the development of the computer Graphical User Interfaces (hereafter GUIs) and more recent work on Ubiquitous and Pervasive Computing.The paper then explores some current themes in HCI with a view to looking for potential overlaps between architectural design and new trends in the design of computational systems