Towards Sustainable Development of Small-Scale Fisheries in the Philippines: Experiences and Lessons Learned from Eight Regional Sites

The focus of this paper is on the governance of small-scale or municipal fisheries in the Philippines in light of the critical role they play in the livelihoods of coastal communities and in the nation as a whole. The information and insights presented in this lessons learned brief derive from the project entitled Strengthening Governance and Sustainability of Small-Scale Fisheries Management in the Philippines: An Ecosystem Approach. The project was funded principally by the Department of Agriculture's Bureau of Agricultural Research (DA-BAR), and implemented from 2008 to 2011 by WorldFish in collaboration with the Department of Science and Technology (DOST) and selected partners. The underlying project's goal was to 'strengthen governance and sustainability of small-scale fisheries management in the Philippines.' There were a variety of objectives spread across two project phases but the primary objectives relevant to this brief include: (1) identifying issues at project sites and assessing potential for an ecosystem based approach to fisheries management, and (2) assessing current fisheries management practices at different levels of governance and identifying best practices. The purposes of this paper are twofold. First, it aims to provide brief highlights of the project findings; second, it aims to present the lessons learned in project implementation covering substantive sectoral concerns as well as methodological issues. It wraps up with some strategic directions that need to be undertaken to reverse the deteriorating conditions of small-scale fisheries (SSF) while at the same time promoting their sustainable development

Fisheries rehabilitation in post-tsunami Aceh: Status and needs from participatory appraisals

The widespread and long-term nature of the tsunami damage in Aceh province, Indonesia has threatened the continued use of coastal and fisheries resources. This article describes the application of the Rapid Appraisal of Fisheries Management System (RAFMS) methodology and presents key findings from the participatory appraisals in 15 study sites. The focus is on changes in the number and types of fishing boats and fishing effort, consumption and marketing flow patterns and community perspectives on livelihood options. The level of aid (for new boats), mainly from international organizations, has been unevenly distributed with the number of boats in 13 of 15 villages still being well below the pre-tsunami levels. A focus on supplying small vessels may put increased fishing pressure on the near-shore zone. Consumption data and marketing flows suggest that most fishing villages are supplying outside markets and adding considerably to the wider food security of the province. Despite the tsunami, marine fisheries-related livelihoods are still preferred, although there are indications for the potential expansion of livelihoods into the culture of new species. Alternative resource-based livelihoods need to be tested and refined to fit the needs of the current conditions in Aceh to provide viable options for eliminating hunger and reducing poverty

Fisheries rehabilitation in post-tsunami Aceh: Status and needs from participatory appraisals

The widespread and long-term nature of the tsunami damage in Aceh province, Indonesia has threatened the continued use of coastal and fisheries resources. This article describes the application of the Rapid Appraisal of Fisheries Management System (RAFMS) methodology and presents key findings from the participatory appraisals in 15 study sites. The focus is on changes in the number and types of fishing boats and fishing effort, consumption and marketing flow patterns and community perspectives on livelihood options. The level of aid (for new boats), mainly from international organizations, has been unevenly distributed with the number of boats in 13 of 15 villages still being well below the pre-tsunami levels. A focus on supplying small vessels may put increased fishing pressure on the near-shore zone. Consumption data and marketing flows suggest that most fishing villages are supplying outside markets and adding considerably to the wider food security of the province. Despite the tsunami, marine fisheries-related livelihoods are still preferred, although there are indications for the potential expansion of livelihoods into the culture of new species. Alternative resource-based livelihoods need to be tested and refined to fit the needs of the current conditions in Aceh to provide viable options for eliminating hunger and reducing poverty.Disaster, Tsunami

Acquisition of pneumococci specific effector and regulatory Cd4+ T cells localising within human upper respiratory-tract mucosal lymphoid tissue

The upper respiratory tract mucosa is the location for commensal Streptococcus (S.) pneumoniae colonization and therefore represents a major site of contact between host and bacteria. The CD4(+) T cell response to pneumococcus is increasingly recognised as an important mediator of immunity that protects against invasive disease, with data suggesting a critical role for Th17 cells in mucosal clearance. By assessing CD4 T cell proliferative responses we demonstrate age-related sequestration of Th1 and Th17 CD4(+) T cells reactive to pneumococcal protein antigens within mucosal lymphoid tissue. CD25(hi) T cell depletion and utilisation of pneumococcal specific MHCII tetramers revealed the presence of antigen specific Tregs that utilised CTLA-4 and PDL-1 surface molecules to suppress these responses. The balance between mucosal effector and regulatory CD4(+) T cell immunity is likely to be critical to pneumococcal commensalism and the prevention of unwanted pathology associated with carriage. However, if dysregulated, such responses may render the host more susceptible to invasive pneumococcal infection and adversely affect the successful implementation of both polysaccharide-conjugate and novel protein-based pneumococcal vaccines

Transient Nature of Long-Term Nonprogression and Broad Virus-Specific Proliferative T-Cell Responses with Sustained Thymic Output in HIV-1 Controllers

HIV-1(+) individuals who, without therapy, conserve cellular anti-HIV-1 responses, present with high, stable CD4(+) T-cell numbers, and control viral replication, facilitate analysis of atypical viro-immunopathology. In the absence of universal definition, immune function in such HIV controllers remains an indication of non-progression.CD4 T-cell responses to a number of HIV-1 proteins and peptide pools were assessed by IFN-gamma ELISpot and lymphoproliferative assays in HIV controllers and chronic progressors. Thymic output was assessed by sjTRECs levels. Follow-up of 41 HIV-1(+) individuals originally identified as "Long-term non-progressors" in 1996 according to clinical criteria, and longitudinal analysis of two HIV controllers over 22 years, was also performed. HIV controllers exhibited substantial IFN-gamma producing and proliferative HIV-1-specific CD4 T-cell responses to both recombinant proteins and peptide pools of Tat, Rev, Nef, Gag and Env, demonstrating functional processing and presentation. Conversely, HIV-specific T-cell responses were limited to IFN-gamma production in chronic progressors. Additionally, thymic output was approximately 19 fold higher in HIV controllers than in age-matched chronic progressors. Follow-up of 41 HIV-1(+) patients identified as LTNP in 1996 revealed the transitory characteristics of this status. IFN-gamma production and proliferative T-cell function also declines in 2 HIV controllers over 22 years.Although increased thymic output and anti-HIV-1 T-cell responses are observed in HIV controllers compared to chronic progressors, the nature of nonprogressor/controller status appears to be transitory

Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients

<p>Abstract</p> <p>Background</p> <p>T-cell immunodeficiency is a common feature in cancer patients, which may relate to initiation and development of tumor. Based on our previous finding, to further characterize the immune status, T cell proliferative history was analyzed in CD4+ and CD8+ T cells from chronic myeloid leukemia (CML) patients.</p> <p>Methods</p> <p>Quantitative analysis of δRec-ψJα signal joint T cell receptor excision circles (sjTRECs) was performed in PBMCs, CD3+, CD4+ and CD8+T cells by real-time PCR, and the analysis of 23 <it>TRBV-D1 </it>sjTRECs was performed by semi-nested PCR. Forty eight CML cases in chronic phase (CML-CP) were selected for this study and 17 healthy individuals served as controls.</p> <p>Results</p> <p>The levels of δRec-ψJα sjTRECs in PBMCs, CD3+, CD4+, and CD8+ T cells were significantly decreased in CML patients, compared with control groups. Moreover, the numbers of detectable <it>TRBV </it>subfamily sjTRECs, as well as the frequency of particular <it>TRBV-BD</it>1 sjTRECs in patients with CML were significantly lower than those from healthy individuals.</p> <p>Conclusions</p> <p>We observed decreased levels of recent thymic emigrants in CD4+ and CD8+ T cells that may underlay the persistent immunodeficiency in CML patients.</p

Exposure to Apoptotic Activated CD4+ T Cells Induces Maturation and APOBEC3G- Mediated Inhibition of HIV-1 Infection in Dendritic Cells

Dendritic cells (DCs) are activated by signaling via pathogen-specific receptors or exposure to inflammatory mediators. Here we show that co-culturing DCs with apoptotic HIV-infected activated CD4+ T cells (ApoInf) or apoptotic uninfected activated CD4+ T cells (ApoAct) induced expression of co-stimulatory molecules and cytokine release. In addition, we measured a reduced HIV infection rate in DCs after co-culture with ApoAct. A prerequisite for reduced HIV infection in DCs was activation of CD4+ T cells before apoptosis induction. DCs exposed to ApoAct or ApoInf secreted MIP-1α, MIP-1β, MCP-1, and TNF-α; this effect was retained in the presence of exogenous HIV. The ApoAct-mediated induction of co-stimulatory CD86 molecules and reduction of HIV infection in DCs were partially abrogated after blocking TNF-α using monoclonal antibodies. APOBEC3G expression in DCs was increased in co-cultures of DCs and ApoAct but not by apoptotic resting CD4+ T cells (ApoRest). Silencing of APOBEC3G in DC abrogated the HIV inhibitory effect mediated by ApoAct. Sequence analyses of an env region revealed significant induction of G-to-A hypermutations in the context of GG or GA dinucleotides in DNA isolated from DCs exposed to HIV and ApoAct. Thus, ApoAct-mediated DC maturation resulted in induction of APOBEC3G that was important for inhibition of HIV-infection in DCs. These findings underscore the complexity of differential DC responses evoked upon interaction with resting as compared with activated dying cells during HIV infection

Trends in the incidence of primary liver cancer in Central Uganda, 1960–1980 and 1991–2005

Primary liver cancer (PLC) incidence trends from Africa are unknown. Using Kampala Cancer Registry data from 1960 to 1980 and 1991 to 2005, we identified 771 PLCs. Although rates were stable among men, PLC incidence among women increased >50%. Investigations of viral hepatitis, aflatoxin, obesity, and human immunodeficiency virus (HIV) may help to explain the increasing incidence of hepatocellular carcinomas (HCCs)

The Contribution of Viral Genotype to Plasma Viral Set-Point in HIV Infection

Disease progression in HIV-infected individuals varies greatly, and while the environmental and host factors influencing this variation have been widely investigated, the viral contribution to variation in set-point viral load, a predictor of disease progression, is less clear. Previous studies, using transmission-pairs and analysis of phylogenetic signal in small numbers of individuals, have produced a wide range of viral genetic effect estimates. Here we present a novel application of a population-scale method based in quantitative genetics to estimate the viral genetic effect on set-point viral load in the UK subtype B HIV-1 epidemic, based on a very large data set. Analyzing the initial viral load and associated pol sequence, both taken before anti-retroviral therapy, of 8,483 patients, we estimate the proportion of variance in viral load explained by viral genetic effects to be 5.7% (CI 2.8-8.6%). We also estimated the change in viral load over time due to selection on the virus and environmental effects to be a decline of 0.05 log10 copies/mL/year, in contrast to recent studies which suggested a reported small increase in viral load over the last 20 years might be due to evolutionary changes in the virus. Our results suggest that in the UK epidemic, subtype B has a small but significant viral genetic effect on viral load. By allowing the analysis of large sample sizes, we expect our approach to be applicable to the estimation of the genetic contribution to traits in many organisms