Radiation Hardness Tests Of Piezoelectric Actuators With Fast Neutrons At Liquid Helium Temperature

Piezoelectric actuators, which are integrated into the cold tuning system and used to compensate the small mechanical deformations of the cavity wall induced by Lorentz forces due to the high electromagnetic surface field, may be located in the radiation environment during particle accelerator operation. In order to provide for a reliable operation of the accelerator, the performance and life time of piezoelectric actuators (~24.000 units for ILC) should not show any significant degradation for long periods (i.e. machine life duration: ~20 years), even when subjected to intense radiation (i.e. gamma rays and fast neutrons). An experimental program, aimed at investigating the effect of fast neutrons radiation on the characteristics of piezoelectric actuators at liquid helium temperature (i.e. T~4.2 K), was proposed for the working package WP#8 devoted to tuners development in the frame of CARE project. A neutrons irradiation facility, already installed at the CERI cyclotron located at Orléans (France), was upgraded and adapted for actuators irradiations tests purpose. A deuterons beam (maximum energy and beam current: 25 MeV and 35µA) collides with a thin (thickness: 3 mm) beryllium target producing a high neutrons flux with low gamma dose (~20%): a neutrons fluence of more than 1014 n/cm2 is achieved in ~20 hours of exposure. A dedicated cryostat was developed at IPN Orsay and used previously for radiation hardness test of calibrated cryogenic thermometers and pressure transducers used in LHC superconducting magnets. This cryostat could be operated either with liquid helium or liquid argon. This irradiation facility was upgraded for allowing fast turn-over of experiments and a dedicated experimental set-up was designed, fabricated, installed at CERI and successfully operated for radiation hardness tests of several piezoelectric actuators at T~4.2 K. This new apparatus allows on-line automatic measurements of actuators characteristics and the cryogenic parameters. Further, the test-cell and actuators are equipped with high purity Ni foils for measuring the total neutrons dose by an activation method. In this report, the details of the irradiation test facility will be described then the experimental data will be analyzed and discussed

Electromechanical, Thermal Properties And Radiation Hardness Tests Of Piezoelectric Actuators At Low Temperature

IPN Orsay participates, in the frame of the CARE project activities supported by EU, to the development of a fast cold tuning system for SRF cavities. The main task of IPN is the full characterization of piezoelectric actuators at low temperature T, and the study of their behaviour when subjected to fast neutrons radiation at T=4.2 K. In order to compare the performance of various industrial piezoelectric actuators, a new apparatus was developed and successfully used for measuring their electromechanical and thermal properties for T in the range 1.8 K-300 K. Different parameters were investigated as function of T: piezoelectric constant, dielectric and thermal properties including heating ΔT due to dielectric losses vs. modulating voltage Vmod and frequency f. We observed a decrease of the maximum displacement ΔX of the actuators tested from ΔX ~40μm @ 300K down to 1.8μm-3.5 μm @ 1.8K, depending on both material and fabrication process of the piezostacks. Besides, both material and fabrication process have a strong influence on the shape of the characteristics ΔX vs. T dependence. Finally a dedicated facility located at CERI institute (Orléans, France) for radiation hardness tests of actuators with fast neutrons at T=4.2 K was developed and the first beam tests results are summarized

Somatic embryogenesis and plant regeneration of Vitis vinifera cultivars 'Macabeo' and 'Tempranillo'

Different experimental conditions have been compared to achieve a high efficiency in embryogenic calli initiation from 'Macabeo' and 'Tempranillo' anthers. Specifically, two stages of anther development were tested (corresponding to tetrad cells or uninucleate pollen), and direct culture of anthers was compared to culture after a cold treatment of inflorescences (4 °C during 48 h). In addition, two induction media (C1 P and B2), mainly differing by microelement and cytokinin levels, were evaluated. Experiment repeatability was also examined with a repetition of anther culture one week later. Callus initiation was similar in all media and treatments for both cultivars, usually starting from the anther filament. A simple protocol for efficient induction of embryogenesis in 'Macabeo' and 'Tempranillo' consisted in:selecting the first inflorescence from hardwood cutting,excising anthers at uninucleate pollen stage without cold treatment of the inflorescences,incubating anthers on C1 P medium.The procedure used for embryo germination and plant regeneration, allowed to obtain a conversion rate up to 75 % in 'Macabeo' and 60 % in 'Tempranillo'. The protocol proposed represents the first regeneration system developed for the Spanish cultivars 'Macabeo' and 'Tempranillo'.

Clinical impact of anti-inflammatory microglia and macrophage phenotypes at glioblastoma margins

Glioblastoma is a devastating brain cancer for which effective treatments are required. Tumour-associated microglia and macrophages promote glioblastoma growth in an immune-suppressed microenvironment. Most recurrences occur at the invasive margin of the surrounding brain, yet the relationships between microglia/macrophage phenotypes, T cells and programmed death-ligand 1 (an immune checkpoint) across human glioblastoma regions are understudied. In this study, we performed a quantitative immunohistochemical analysis of 15 markers of microglia/macrophage phenotypes (including anti-inflammatory markers triggering receptor expressed on myeloid cells 2 and CD163, and the low-affinity-activating receptor CD32a), T cells, natural killer cells and programmed death-ligand 1, in 59 human IDH1-wild-type glioblastoma multi-regional samples (n = 177; 1 sample at tumour core, 2 samples at the margins: the infiltrating zone and leading edge). Assessment was made for the prognostic value of markers; the results were validated in an independent cohort. Microglia/macrophage motility and activation (Iba1, CD68), programmed death-ligand 1 and CD4+ T cells were reduced, and homeostatic microglia (P2RY12) were increased in the invasive margins compared with the tumour core. There were significant positive correlations between microglia/macrophage markers CD68 (phagocytic)/triggering receptor expressed on myeloid cells 2 (anti-inflammatory) and CD8+ T cells in the invasive margins but not in the tumour core (P < 0.01). Programmed death-ligand 1 expression was associated with microglia/macrophage markers (including anti-inflammatory) CD68, CD163, CD32a and triggering receptor expressed on myeloid cells 2, only in the leading edge of glioblastomas (P < 0.01). Similarly, there was a positive correlation between programmed death-ligand 1 expression and CD8+ T-cell infiltration in the leading edge (P < 0.001). There was no relationship between CD64 (a receptor for autoreactive T-cell responses) and CD8+/CD4+ T cells, or between the microglia/macrophage antigen presentation marker HLA-DR and microglial motility (Iba1) in the tumour margins. Natural killer cell infiltration (CD335+) correlated with CD8+ T cells and with CD68/CD163/triggering receptor expressed on myeloid cells 2 anti-inflammatory microglia/macrophages at the leading edge. In an independent large glioblastoma cohort with transcriptomic data, positive correlations between anti-inflammatory microglia/macrophage markers (triggering receptor expressed on myeloid cells 2, CD163 and CD32a) and CD4+/CD8+/programmed death-ligand 1 RNA expression were validated (P < 0.001). Finally, multivariate analysis showed that high triggering receptor expressed on myeloid cells 2, programmed death-ligand 1 and CD32a expression at the leading edge were significantly associated with poorer overall patient survival (hazard ratio = 2.05, 3.42 and 2.11, respectively), independent of clinical variables. In conclusion, anti-inflammatory microglia/macrophages, CD8+ T cells and programmed death-ligand 1 are correlated in the invasive margins of glioblastoma, consistent with immune-suppressive interactions. High triggering receptor expressed on myeloid cells 2, programmed death-ligand 1 and CD32a expression at the human glioblastoma leading edge are predictors of poorer overall survival. Given substantial interest in targeting microglia/macrophages, together with immune checkpoint inhibitors in cancer, these data have major clinical implications

Understanding the Distributions of Benthic Foraminifera in the Adriatic Sea with Gradient Forest and Structural Equation Models

Abstract: In the last three decades, benthic foraminiferal ecology has been intensively investigated to improve the potential application of these marine organisms as proxies of the effects of climate change and other global change phenomena. It is still challenging to define the most important factors affecting foraminiferal communities and derived faunistic parameters. In this study, we examined the abiotic-biotic relationships of foraminiferal communities in the central-southern area of the Adriatic Sea using modern machine learning techniques. We combined gradient forest (Gf) and structural equation modeling (SEM) to test hypotheses about determinants of benthic foraminiferal assemblages. These approaches helped determine the relative effect of sizes of different environmental variables responsible for shaping living foraminiferal distributions. Four major faunal turnovers (at 13–28 m, 29–58 m, 59–215 m, and &gt;215 m) were identified along a large bathymetric gradient (13–703 m water depth) that reflected the classical bathymetric distribution of benthic communities. Sand and organic matter (OM) contents were identified as the most relevant factors influencing the distribution of foraminifera either along the entire depth gradient or at selected bathymetric ranges. The SEM supported causal hypotheses that focused the factors that shaped assemblages at each bathymetric range, and the most notable causal relationships were direct effects of depth and indirect effects of the Gf-identified environmental parameters (i.e., sand, pollution load Index–PLI, organic matter–OM and total nitrogen–N) on foraminifera infauna and diversity. These results are relevant to understanding the basic ecology and conservation of foraminiferal communitie

Characteristics of air showers created by extremely high energy gamma-rays

The technique of adjoint cascade equations has been applied to calculate the properties of extremely high energy gamma-rays in the energy range 10^18--10^22 eV with taking into account the LPM effect and interactions of gamma-rays with the geomagnetic field. Such characteristics are analysed as the electron and muon contents at the observation level, the electron cascade curves, the lateral distribution functions of photoproduced muons.Comment: 36 pages, 19 figures, submitted to J.Phys.G: Nucl.Part.Phy

Phthalates: Potential sources and control measures

The issue of endocrine disruptors is a subject of debate in the agri-food sector and questioning for consumers through the media. Among these compounds, some of the family of phthalates, are used in the composition of some materials used in wineries. This makes it a worrying issue in terms of image and safety. The objective ok this work was to identify the sources of phthalates and the factors favoring their diffusion in wines to propose preventive and curative solutions to the wine makers. The modalities were therefore selected in different French wine regions to represent the diversity of oenological practices. This survey also led to examine more thoroughly the parameters involved in the situations that favored migration of the highest levels. Additional experiments were performed specifically on certain sources or contributing factors. The first identified sources include epoxy resin, fiberglass reinforced polyester as well as PVC materials (pipes …). Favorable factors include alcohol content, temperature, duration and contact area

Physical properties and biocompatibility of Chitosan/soy blended membranes

Blends of polysaccharides and proteins are a source for the development of novel materials with interesting and tailorable properties, with potential to be used in a range of biomedical applications. in this work a series of blended membranes composed by chitosan and soy protein isolate was prepared by solvent casting methodology. in addition, cross-linking was performed in situ with glutaraldehyde solutions in the range 5 × 10–3 – 0.1 M. Furthermore, the influence of the composition and cross-linking on the degradation behaviour, water uptake and cell adhesion was investigated. The obtained results showed that the incorporation of chitosan, associated to network formation by cross linking, promoted a slight decrease of water absorption and a slower degradability of the membranes. Moreover, direct contact biocompatibility studies, with L929 cells, indicate that the cross-linking enhances the capability of the material to support cell growth.Fundação para a Ciência e a Tecnologia (FCT