2,209 research outputs found
Landscape as mediator, landscape as commons: an introduction
Il contributo propone una trattazione dei due temi chiavi che si intersecano nel volume, andando a costituire non solo una introduzione ai saggi ivi contenuti, ma una pi\uf9 ampia trattazione delle questioni attorno a cui questi si muovono: le potenzialit\ue0 del concetto di paesaggio considerato come intermediario/mediatore e come bene comune/commons. Il volume raccoglie i migliori contributi internazionali presentati nelle sessioni sul paesaggio del congresso Eugeo 2013 (Roma) coordinate dai curatori dell'opera, e si conclude con una approfondita postfazione redatta da Kenneth R. Olwig, che ha partecipato come discussant ai lavori congressuali
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Use of genetic markers for the detection of off-types for DUS phenotypic traits in the inbreeding crop, barley
Detection of crop off-types is of interest for
multiple uses, including the assessment of uniformity
for new plant variety applications during distinctness,
uniformity and stability (DUS) testing for the awarding
of plant breeders’ rights (PBR). Here, we investigate
whether genetic markers, in this case Kompetitive
Allele-Specific PCR (KASP), can be used for the identification
off-types for phenotypes assessed for DUS in
the inbreeding cereal crop, barley (Hordeum vulgare).
To demonstrate proof of principle, KASP markers diagnostic
for phenotypic expression of nine DUS phenotypes,
and DNA from two barley varieties (‘Pelican’ and
‘Felicie’) carrying contrasting alleles at each marker
were used. We found that for the majority of markers,
it was possible to robustly call alleles down to template
DNA concentrations of 2 ng, but not ≤ 0.2 ng. When
used in mixtures of DNA consisting of ‘Felicie’ DNA
spiked with different concentrations of ‘Pelican’ DNA,
robust allele calling was possible in DNA mixtures
down to 18 ng:2 ng. Collectively, this demonstrates that
where diagnostic markers are available, molecular identification
of a single off-type for a given DUS trait
within a bulk of ten individuals should be possible. We
validated this assumption, with all of the diagnostic
genetic markers investigated found to robustly detect
DUS off-types at a frequency of 10% in DNA extracted
from tissue collected from pools of 10 individuals. Ultimately,
this work demonstrates that, where diagnostic
polymorphisms are known for DUS traits, KASP
markers should be able to robustly detect off-types or
cross-contamination within DNA samples from a diploid
inbred species down to 10%. While just two varieties
that contrasted for the eight DUS targeted were
investigated in this study, as the markers used are diagnostic
for their relevant phenotype (or a proportion of
the variation observed for that phenotype), in theory the
approach should be valid for any variety studied—
although the introduction of novel alleles via spontaneous
mutation or more exotic germplasm pools may
mean that marker sets would need to be periodically
added to or updated. However, we nevertheless demonstrate
the principle that, for a subset of DUS traits,
molecular markers can now be robustly used as a tool
towards determining all three components of the DUS
testing process in barley. These results are relevant for
the assessment of varietal uniformity by crop breeders,
crop testing authorities and germplasm maintenance, as
well as highlighting the potential use of bulk samples
rather than individual plant samples for assessment of
distinctness by molecular methods
Clitics in imperative: proclisis, enclisis and mesoclisis in Albanian and in Italo-Romance varieties of Lausberg area.
The relation between morpho-syntactic structure and its externalization into interpretive levels is the topic of this article. In many languages, typically in Romance and Albanian varieties, modal contexts, specifically imperative and infinitive, and negation, give rise to phenomena of clitic reordering and an interesting micro-variation. Imperative differs from declarative sentences in selecting enclisis except in negative contexts. Moreover, in Albanian mesoclisis appears in the 2nd plural person of imperative, between the verbal base and the person inflection. A similar distribution characterizes Calabro-Lucanian varieties spoken in Lausberg area, in contact with Arbëresh (Italo-Albanian) dialects. This article proposes to analyze the influence of modal contexts on the lexicalization of object clitics (OCls) and their different behavior in connection to their referential properties. Our descriptive and theoretical starting point is the representational morpho-syntactic approach adopted in Manzini and Savoia (2011 and subsequent works; see Section 5)
From blue star-forming to red passive: galaxies in transition in different environments
Exploiting a mass complete (M_*>10^(10.25)M_sun) sample at 0.03<z<0.11 drawn
from the Padova Millennium Galaxy Group Catalog (PM2GC), we use the (U-B)_rf
color and morphologies to characterize galaxies, in particular those that show
signs of an ongoing or recent transformation of their star formation activity
and/or morphology - green galaxies, red passive late types, and blue
star-forming early types. Color fractions depend on mass and only for
M_*<10^(10.7)M_sun on environment. The incidence of red galaxies increases with
increasing mass, and, for M_*<10^(10.7)M_sun, decreases toward the group
outskirts and in binary and single galaxies. The relative abundance of green
and blue galaxies is independent of environment, and increases monotonically
with galaxy mass. We also inspect galaxy structural parameters, star-formation
properties, histories and ages and propose an evolutionary scenario for the
different subpopulations. Color transformations are due to a reduction and
suppression of SFR in both bulges and disks which does not noticeably affect
galaxy structure. Morphological transitions are linked to an enhanced
bulge-to-disk ratio due to the removal of the disk, not to an increase of the
bulge. Our modeling suggests that green colors might be due to star formation
histories declining with long timescales, as an alternative scenario to the
classical "quenching" processes. Our results suggest that galaxy
transformations in star formation activity and morphology depend neither on
environment nor on being a satellite or the most massive galaxy of a halo. The
only environmental dependence we find is the higher fast quenching efficiency
in groups giving origin to post-starburst signatures.Comment: 20 pages, 12 figures, accepted for publication in Ap
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