Efficacy of oral appliance therapy in patients following uvulopalatopharyngoplasty failure

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/149311/1/lio2256.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/149311/2/lio2256_am.pd

Tolerância à seca em forrageiras tropicais - Aspectos agronômicos e microbiológicos.

Gramíneas. Leguminosas; Método de seleção de plantas para a seca, através da germinação de sementes em solução de sacarose.bitstream/CNPAB-2010/27202/1/doc009.pd

Sombreamento em forrageiras - Aspectos agronômicos e microbiológicos.

Efeito do sombreamento no crescimento de forrageiras. Rendimento de matéria seca da parte aérea. Porcentagem de matéria seca (M.S) da parte aérea. Rendimento de MS da raiz. Índice de área foliar, relação área foliar e peso e altura da planta. Porcentagem de nitrogênio (N%). Rendimento de N total. Composição mineral. P. K. Ca e Mg. S. Cu e Zn. Porcentagem do N recuperado. Retorno de nutrientes ao solo. Efeito do sombreamento na fixação biológica de nitrogênio.bitstream/CNPAB-2010/27204/1/doc010.pd

Should Endodontic Residents Be Educated About IV‐Sedation? Endodontics Program Directors’ and Endodontists’ Perspectives

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/153555/1/jddjde018077.pd

Efeito do uso da cama de frango no estabelecimento da alfafa (Medicago sativa cv. Crioulo) em Paty de Alfares.

bitstream/CNPAB-2010/27147/1/cot022.pd

Minimally Invasive Approach for Diagnosing TMJ Osteoarthritis

This study’s objectives were to test correlations among groups of biomarkers that are associated with condylar morphology and to apply artificial intelligence to test shape analysis features in a neural network (NN) to stage condylar morphology in temporomandibular joint osteoarthritis (TMJOA). Seventeen TMJOA patients (39.9 ± 11.7 y) experiencing signs and symptoms of the disease for less than 10 y and 17 age- and sex-matched control subjects (39.4 ± 15.2 y) completed a questionnaire, had a temporomandibular joint clinical exam, had blood and saliva samples drawn, and had high-resolution cone beam computed tomography scans taken. Serum and salivary levels of 17 inflammatory biomarkers were quantified using protein microarrays. A NN was trained with 259 other condyles to detect and classify the stage of TMJOA and then compared to repeated clinical experts’ classifications. Levels of the salivary biomarkers MMP-3, VE-cadherin, 6Ckine, and PAI-1 were correlated to each other in TMJOA patients and were significantly correlated with condylar morphological variability on the posterior surface of the condyle. In serum, VE-cadherin and VEGF were correlated with one another and with significant morphological variability on the anterior surface of the condyle, while MMP-3 and CXCL16 presented statistically significant associations with variability on the anterior surface, lateral pole, and superior-posterior surface of the condyle. The range of mouth opening variables were the clinical markers with the most significant associations with morphological variability at the medial and lateral condylar poles. The repeated clinician consensus classification had 97.8% agreement on degree of degeneration within 1 group difference. Predictive analytics of the NN’s staging of TMJOA compared to the repeated clinicians’ consensus revealed 73.5% and 91.2% accuracy. This study demonstrated significant correlations among variations in protein expression levels, clinical symptoms, and condylar surface morphology. The results suggest that 3-dimensional variability in TMJOA condylar morphology can be comprehensively phenotyped by the NN

Lipid droplet levels vary heterogeneously in response to simulated gastrointestinal stresses in different probiotic Saccharomyces cerevisiae strains

AbstractTo exert their therapeutic action, probiotic Saccharomyces cerevisiae strains must survive harsh digestive environments. Lipid droplets accumulate in cells which undergo stress-inducing situations, supposedly having a protective role. We assessed lipid droplet levels, either naturally accumulated or induced in response to digestive challenges, of probiotic strains S. boulardii, S. cerevisiae A-905, S. cerevisiae Sc47 and S. cerevisiae L11, and of non-probiotic strains S. cerevisiae BY4741 and S. cerevisiae BY4743. Strains 905 and Sc47 had lower and higher lipid droplet levels, respectively, when compared to the remaining strains, showing that higher accumulationof these neutral lipids is not a feature shared by all probiotic Saccharomyces strains. When submitted to simulated gastric or bile salts environments, lipid droplet levels increase in all tested probiotic strains, at least for one to the induced stresses, suggesting that lipid droplets participate in the protective mechanisms against gastrointestinal stresses in probiotic Saccharomyces yeasts

Combination of Sleeping Beauty transposition and chemically induced dimerization selection for robust production of engineered cells

The main methods for producing genetically engineered cells use viral vectors for which safety issues and manufacturing costs remain a concern. In addition, selection of desired cells typically relies on the use of cytotoxic drugs with long culture times. Here, we introduce an efficient non-viral approach combining the Sleeping Beauty (SB) Transposon System with selective proliferation of engineered cells by chemically induced dimerization (CID) of growth factor receptors. Minicircles carrying a SB transposon cassette containing a reporter transgene and a gene for the F36VFGFR1 fusion protein were delivered to the hematopoietic cell line Ba/F3. Stably-transduced Ba/F3 cell populations with >98% purity were obtained within 1 week using this positive selection strategy. Copy number analysis by quantitative PCR (qPCR) revealed that CID-selected cells contain on average higher copy numbers of transgenes than flow cytometry-selected cells, demonstrating selective advantage for cells with multiple transposon insertions. A diverse population of cells is present both before and after culture in CID media, although site-specific qPCR of transposon junctions show that population diversity is significantly reduced after selection due to preferential expansion of clones with multiple integration events. This non-viral, positive selection approach is an attractive alternative for producing engineered cells