Immunohistochemical localization of mdm-2, p27Kip1 and bcl-2 in Warthin's tumor of the parotid gland

<p>Abstract</p> <p>Background</p> <p>Warthin's tumor is a benign monomorphic adenoma with unclear origin that almost occur exclusively in the parotid gland. Etiology of Warthin's tumor as well as its malignant potential are still unclear. Therefore immunohistochemical assessment of Warthin's tumor may be useful to detect its origin or its malignant transformation potential.</p> <p>Aims and objectives</p> <p>The present study aims to investigate the immunohistochemical expression of murine double minute-2 (mdm-2), p27^Kip1and B cell lymphoma-2 (bcl-2) in Warthin's tumor of parotid gland and also to clarify the role of these proteins in the behavior of that tumor.</p> <p>Methods</p> <p>Twenty paraffin blocks of cases previously diagnosed as Warthin's tumor were collected for immunohistochemical staining with primary antibodies against mdm-2, p27^Kip1and bcl-2 using streptavidin-biotin immunoperoxidase staining system.</p> <p>Results</p> <p>All cases showed immunopositivity for mdm-2 and p27^Kip1while 18/20 showed bcl-2 immunopositivity. Both layers of the neoplastic epithelial cells that line the cystic spaces showed immunopositivity with all antibodies used. Goblet cells were mdm-2 immunonegative while myoepithelial cells were p27^Kip-1immunonegative. Areas of epithelial proliferation that formed buds were p27^Kip-1and bcl-2 immunopositive.</p> <p>Conclusion</p> <p>Mdm-2 played a tumor-suppressor role that might be implicated with the benign behavior of Warthin's tumor. The mutual expression of both p27^Kip1and bcl-2 suggested a protective role of these slowly proliferating cells from apoptosis to maintain their survival and elevated bcl-2 expression offers a significant protection against p27^Kip1-mediated apoptosis.</p

Cyber-Physical Systems: A Model-Based Approach

In this concise yet comprehensive Open Access textbook, future inventors are introduced to the key concepts of Cyber-Physical Systems (CPS). Using modeling as a way to develop deeper understanding of the computational and physical components of these systems, one can express new designs in a way that facilitates their simulation, visualization, and analysis. Concepts are introduced in a cross-disciplinary way. Leveraging hybrid (continuous/discrete) systems as a unifying framework and Acumen as a modeling environment, the book bridges the conceptual gap in modeling skills needed for physical systems on the one hand and computational systems on the other. In doing so, the book gives the reader the modeling and design skills they need to build smart, IT-enabled products. Starting with a look at various examples and characteristics of Cyber-Physical Systems, the book progresses to explain how the area brings together several previously distinct ones such as Embedded Systems, Control Theory, and Mechatronics. Featuring a simulation-based project that focuses on a robotics problem (how to design a robot that can play ping-pong) as a useful example of a CPS domain, Cyber-Physical Systems: A Model-Based Approach demonstrates the intimate coupling between cyber and physical components, and how designing robots reveals several non-trivial control problems, significant embedded and real-time computation requirements, and a need to consider issues of communication and preconceptions

Graphene Functionalization towards Developing Superior Supercapacitors Performance

Graphene is known as the miracle material of the 21st century for the wide band of participating applications and epic properties. Unlike the CVD monolayer graphene, Reduced graphene oxide (RGO) is a commercial form with mass production accessibility via numerous numbers of methods in preparation and reduction terms. Such RGO form showed exceptional combability in supercapacitors (SCs) where RGO is participated to promote flexibility, lifetime and performance. The chapter will illustrate 4 critical milestones of using graphene derivatives for achieving SC’s superior performance. The first is using oxidized graphene (GO) blind with polymer for super dielectric spacer. The other three types are dealing with electrolytic SCs based on RGO. Polyaniline (PANI) was grown on GO for exceptionally stable SCs of 100% retention. Silver decoration of RGO was used for all-solid-state printable device. The solid-state gel electrolyte was developed by adding GO to promote current rating. Finally, laser reduced graphene is presented as a one-step and versatile technique for micropatterning processing. The RGO reduction was demonstrated from a laser GO interaction perspective according to two selected key parameters; wavelength and pulse duration

Cyber-Physical Systems: A Model-Based Approach

In this concise yet comprehensive Open Access textbook, future inventors are introduced to the key concepts of Cyber-Physical Systems (CPS). Using modeling as a way to develop deeper understanding of the computational and physical components of these systems, one can express new designs in a way that facilitates their simulation, visualization, and analysis. Concepts are introduced in a cross-disciplinary way. Leveraging hybrid (continuous/discrete) systems as a unifying framework and Acumen as a modeling environment, the book bridges the conceptual gap in modeling skills needed for physical systems on the one hand and computational systems on the other. In doing so, the book gives the reader the modeling and design skills they need to build smart, IT-enabled products. Starting with a look at various examples and characteristics of Cyber-Physical Systems, the book progresses to explain how the area brings together several previously distinct ones such as Embedded Systems, Control Theory, and Mechatronics. Featuring a simulation-based project that focuses on a robotics problem (how to design a robot that can play ping-pong) as a useful example of a CPS domain, Cyber-Physical Systems: A Model-Based Approach demonstrates the intimate coupling between cyber and physical components, and how designing robots reveals several non-trivial control problems, significant embedded and real-time computation requirements, and a need to consider issues of communication and preconceptions

CD10 and osteopontin expression in dentigerous cyst and ameloblastoma

<p>Abstract</p> <p>Aims and Objectives</p> <p>To investigate the expression of CD10 and osteopontin in dentigerous cyst and ameloblastoma and to correlate their expression with neoplastic potentiality of dentigerous cyst and local invasion and risk of local recurrence in ameloblastoma.</p> <p>Methods</p> <p>CD10 and osteopontin expression was studied by means of immunohistochemistry in 9 cases of dentigerous cysts (DC) and 17 cases of ameloblastoma. There were 7 unicystic ameloblastoma (UCA) and 10 multicystic ameloblastoma (MCA). Positive cases were included in the statistical analysis, carried on the tabulated data using the Open Office Spreadsheet 3.2.1 under Linux operating system. Analysis of variance and correlation studies were performed using "R" under Linux operating system (R Development Core Team (2010). Tukey post-hoc test was also performed as a pair-wise test. The significant level was set at 0.05.</p> <p>Results</p> <p>High CD10 and osteopontin expression was observed in UCA and MCA, and low CD10 and osteopontin expression was observed in DC. Significant correlation was seen between CD10 and osteopontin expression and neoplastic potentiality of DC and local invasion and risk of recurrences in ameloblastoma.</p> <p>Conclusions</p> <p>In DC, high CD10 and osteopontin expression may indicate the neoplastic potentiality of certain areas. In UCA & MCA, high CD10 and osteopontin expression may identify areas with locally invasive behavior and high risk of recurrence.</p

Preparation and Characterization of Magnetite Talc (Fe3_{3}O4_{4}@Talc) Nanocomposite as an Effective Adsorbent for Cr(VI) and Alizarin Red S Dye

In this work, the adsorption of Cr(VI) ions and the organic dye Alizarin Red S (ARS) was investigated using magnetite talc (Fe$_{3}$O$_{4}$@Talc) nanocomposite. Different characterization techniques such as scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray Diffraction (XRD), and thermogravimetric analysis (TGA) were used to demonstrate the physical and chemical properties of the fabricated Fe$_{3}$O$_{4}$@Talc nanocomposite. In addition, the adsorption isothermic, kinetic, and thermodynamic properties were illustrated. The results demonstrate that the investigated adsorption processes obeyed the Langmuir isotherm model for Cr(VI) and the Freundlich isotherm model for ARS dye, with a maximum adsorption capacity of 13.5 and 11.76 mg·g$^{-1}$, respectively, controlled by pseudo second-order kinetics. Regeneration and reusability studies demonstrated that the prepared Fe$_{3}$O$_{4}$@Talc nanocomposite is a promising and stable adsorbent with considerable reusability potential

Histopathological and immunohistochemical study of the protective effect of triptorelin on the neurocytes of the hippocampus and the cerebral cortex of male albino rats after short-term exposure to cyclophosphamide

AbstractChemotherapy treats many types of cancer effectively but it often causes side effects. Chemotherapy works on active cells, such as cancer cells, and some healthy cells. Side effects happen when chemotherapy damages these healthy cells. Today, many more drugs are available to treat side effects than in the past. Triptorelin (Decapeptyl) is a gonadotropin-releasing hormone agonist that is reported to have many therapeutic effects besides being an anti-cancer agent. In the current study, intraperitoneal cyclophosphamide (65mg/kg/day) was administered for 4 weeks to induce marked dystrophic changes in the cerebral cortex and hippocampus of male albino rats. After 4 weeks, we observed significant degeneration of neurocytes with dystrophic changes. Subcutaneous triptorelin (0.05mg/kg/day) for 4 weeks significantly improved histological signs of degeneration and apoptosis. Anti-Bcl2 staining of sections of the cerebral cortex and hippocampus showed that the apoptotic index was increased. This finding was confirmed by the anti-p53 staining, which showed a significant decrease in the apoptotic index. Ultimately, such improvements were accompanied by significant restoration of normal brain histology, as revealed by hematoxylin and eosin. In conclusion, triptorelin can reverse the apoptotic changes induced by cyclophosphamide therapy, which is more marked in the hippocampus than cerebral cortex

A Critical Review of the Performance, Combustion, and Emissions ‎Characteristics of PCCI Engine Controlled by Injection Strategy and ‎Fuel Properties

As internal combustion engines (ICEs) produce serious emissions and a big part of greenhouse gases from fuel combustion. Due to the universal concerns about degradation in the ambient environment, limitations on exhaust emissions, depletion of petroleum reserves, and global warming, many strict regulations have been launched on the standard emissions released from engines. Premixed charge compression ignition (PCCI) is a promising technique to overcome these challenges in recent years which can simultaneously reduce NOx and soot emissions and substantially improve thermal efficiency. The PCCI combustion concept has the advantages of both SI and CI engines, like SI engines as the charge is premixed which produces low emissions and like CI engines the fuel-air mixture is auto-ignited as a result of compression which leads o high thermal efficiency. Normally, PCCI combustion is a single-stage combustion process achieved by employing early injection timing to increase the time available for mixing fuel and air by using single-fuel and split fuel (pilot/main) injection tactics, in which a large fraction of fuel burns in premixed combustion phase resulting in relatively lower in-cylinder temperatures compared to compression ignition (CI) combustion. Thus, the objective of this paper is to provide an inclusive review of the effects of fuel injection timings, ratios, pressure, and fuel properties on the PCCI engine combustion performance improvement and emission reduction, this review has been analyzed extensively based on the published studies to provide and discuss different strategies for the control of PCCI technique of combustion at wide range of speed and load

Synthesis of Ni-Fe-CO3_3 layered double hydroxide as Effective Adsorbent to remove Cr(VI) and ARS-dye from aqueous media

Ni$^2$/Fe$^{3＋}$LDH, (Ni-Fe-CO3 LDH) with Ni/Fe molar ratio 3.0 was synthesized by co-precipitation for the remediation of chromium (VI), and Alizarine Red-S (ARS-dye) as anionic species. The investigated adsorbent was characterized by TGA, SEM, XRD, BET and FTIR. The effect of the hydrogen ion concentration of the medium, shaking time, ARS-dye and/or Cr(VI) concentration and adsorbents mass on the process was studied. The results of Ni-Fe-CO$_3$ LDH fitted well with the pseudo-second-order model. Langmuir isotherm is more favor than the Freundlich isotherm with maximum capacity ($Q_{max}$) of 69.9 and 6.1 mg/g for ARS-dye and Cr(VI), respectively

Biochemical Studies on Moringa Oleifera Leaves Extract

Phytochemical screening were determined in Moringa oleifera leaves. The total polyphenols and flavonoids contents of Moringa oleifera leaves have the highest of total polyphenols and flavonoids contents, which were 129.44mgGAE/g and 20.43mgGAE/g, respectively. Methanolic and aqueous extracts of plant leaves was antioxidant activity by used (FRAP, LPO, OH, DPPH and ABTS) The methanolic extract of Moringa oleifera leaves have the highest of reducing power which was ranged from 0.818 to 3.021 at the concentrations of 20 and 80 mg/ml, respectively. Also, by used (LPO, OH, DPPH and ABTS), were the highest antioxidants activity for methanolic extract respectively. Moreover, The methanolic extract of Moringa oleifera leaves produced the highest growth inhibition (20 and 17mm) for against Escherichia coli and Bacillus subtillis at 4mg/ml, respectively. While, the aqueous extracts highest growth inhibition (13mm) of against St.coccus aureus at 4mg/ml. Keywords: Phytochemical , polyphenols, flavonoids, plant extracts, antioxidant activity and antibacterial