Quality assessment of fish burgers from deep flounder (Pseudorhombus elevatus) and brushtooth lizardfish (Saurida undosquamis) during storage at -18ºC

Microbiological, chemical and sensory changes of fish burgers prepared from deep flounder (Pseudorhombus elevatus) and brushtooth lizardfish (Saurida undosquamis) were determined during storage at -18ºC for 5 months. Microbiological counts were including total plate count (TPC), total coliform (TC), Staphylococcus aureus, Psychotropic and Escherichia coli decreased throughout the frozen storage . Reduction of microbial load in brushtooth lizardfish was higher than that in deep flounder, except for Staphylococcus aureus counts that was almost equal in both groups. There was a significant increase in pH value in both groups (P<0.05) in first and second months of storage only. Moisture content increased in both groups at the end of 5th month, with increase of moisture in deep flounder fish burgers being higher than that in brushtooth lizardfish burgers. TVB-N values in both groups increased significantly (P<0.05 and P<0.008 for deep flounder and brushtooth lizardfish burgers, respectively) at the end of the second month, however, there was a decrease or no significant change afterward. TBA value of deep flounder fish burgers had a significant decrease (P<0.05) as storage time continued, however, it increased significantly in brushtooth lizardfish burgers at the end of second month (P<0.006) following by a decrease at the end of storage period. Peroxide value (PV) in both groups increased significantly (P<0.05 and P<0.002 in deep flounder and brushtooth lizardfish burgers, respectively) during storage time but a significant decrease was observed at the end of third and fourth months (P<0.005 and P<0.001 in deep flounder and brushtooth lizardfish burgers, respectively). Sensory parameters (color, texture, taste and general acceptability) for two groups decreased significantly (P<0.003 for all parameters in 2 groups) during storage with deep flounder fish burgers receiving higher scores than brushtooth lizardfish burgers at the beginning and end of the storage period

Evaluation and comparison of SYBR Green I Real-Time PCR and TaqMan Real-Time PCR methods for quantitative assay of Listeria monocytogenes in nutrient broth and milk

Specific traditional plate count method and real-time PCR systems based on SYBR Green I and TaqMan technologies using a specific primer pair and probe for amplification of iap-gene were used for quantitative assay of Listeria monocytogenes in seven decimal serial dilution series of nutrient broth and milk samples containing 1.58 to 1.58×107 cfu /ml and the real-time PCR methods were compared with the plate count method with respect to accuracy and sensitivity. In this study, the plate count method was performed using surface-plating of 0.1 ml of each sample on Palcam Agar. The lowest detectable level for this method was 1.58×10 cfu/ml for both nutrient broth and milk samples. Using purified DNA as a template for generation of standard curves, as few as four copies of the iap-gene could be detected per reaction with both real-time PCR assays, indicating that they were highly sensitive. When these real-time PCR assays were applied to quantification of L. monocytogenes in decimal serial dilution series of nutrient broth and milk samples, 3.16×10 to 3.16×105 copies per reaction (equals to 1.58×103 to 1.58×107 cfu/ml L. monocytogenes) were detectable. As logarithmic cycles, for Plate Count and both molecular assays, the quantitative results of the detectable steps were similar to the inoculation levels

Radiotherapy to the primary tumour for newly diagnosed, metastatic prostate cancer (STAMPEDE): a randomised controlled phase 3 trial.

Based on previous findings, we hypothesised that radiotherapy to the prostate would improve overall survival in men with metastatic prostate cancer, and that the benefit would be greatest in patients with a low metastatic burden. We aimed to compare standard of care for metastatic prostate cancer, with and without radiotherapy.This article is freely available via Open Access

Soybean and canola oils induced changes in fatty acids’ profile of chicken Lyoner during storage

Chicken sausage is among popular meat products which in turn, has a share in daily diets.Accordingly, study on meat products and the variation of their characteristics during processing and storage conditions are of the uppermost importance. In this study, chicken sausages containing 90% chicken meat were manufactured under similar conditions except for the added oil type. That is to say, the two batches of chicken sausages were manufactured by the addition of canola and soybean oils in their formula. The samples were stored at 4 to 5 °C for 45 days. With 15-day interval, the samples were analyzed for the fatty acid profile (palmitic, stearic, oleic, linoleic and linolenic acids), microbial (total psychrotrophic bacteria and lactobacillus) and chemical [moisture content, pH, peroxide and thiobarbituric acid (TBA) values as well as free fatty acid (FFA)] properties. Except for the peroxide value, all parameters showed statistically significant (

Rapamycin inhibits oxidative/nitrosative stress and enhances angiogenesis in high glucose-treated human umbilical vein endothelial cells: role of autophagy

Chronic hyperglycemia is a potent risk factor of abnormal angiogenesis with various tissue diseases. Autophagy, as an alternative cell response, is mostly generated by a vast array of insults. Applying autophagic response contributes to normal cell retrieval circumstance during various insults. We aimed to show whether stimulation/inhibition of autophagy could reduce or exacerbate oxidative status and angiogenic potential in endothelial cells after exposure to 30&nbsp;mM glucose. HUVECs were incubated with the combined regime of 100&nbsp;nM Rapamycin and 30&nbsp;mM glucose over a period of 72&nbsp;h. The effect of rapamycin on cell viability, malondialdehyde levels, and nitric oxide were monitored by convenient assays. Intracellular ROS level was measured by flow cytometric analysis and DCFDA. HUVECs migration and angiogenic properties were assessed using scratch test and tubulogenesis assay. The expression of autophagic modulators LC3, Becline-1 and P62 was measured by using western blotting. Data showed 30&nbsp;mM glucose reduced cell viability, migration and in vitro tubulogenesis and level of ROS and nitric oxide were found to increased (p&nbsp;&lt;&nbsp;0.05). Rapamycin had potential to increase cell survival and significantly decreased the total levels of oxidative stress markers after cell exposure to 30&nbsp;mM glucose (p&nbsp;&lt;&nbsp;0.05). Rapamycin potentially improved the detrimental effect of 30&nbsp;mM glucose on cell migration and tubulogenesis capacity (p&nbsp;&lt;&nbsp;0.05). Effective autophagic response was stimulated by rapamycin by increasing beclin-1, and the LC3-II/I ratio and reducing intracellular P62 level (p&nbsp;&lt;&nbsp;0.05), resulting in the improvement of cell health and function. Together, rapamycin protected HUVECs from damages caused by high glucose concentration. This effect was possibly mediated by autophagy-dependent pathway

Surface immobilization of engineered nanomaterials for in situ study of their environmental transformations and fate

The transformation and environmental fate of engineered nanomaterials (ENMs) is the focus of intense research due to concerns about their potential impacts in the environment as a result of their uniquely engineered properties. Many approaches are being applied to investigate the complex interactions and transformation processes ENMs may undergo in aqueous and terrestrial environments. However, major challenges remain due to the difficulties in detecting, separating, and analyzing ENMs from environmental matrices. In this work, a novel technique capable of in situ study of ENMs is presented. By exploiting the functional interactions between surface modified silver nanoparticles (AgNPs) and plasma-deposited polymer films, AgNPs were immobilized on to solid supports that can be deployed in the field and retrieved for analysis. Either negatively charged citrate or polyethylene glycol, or positively charged polyethyleneimine were used to cap the AgNPs, which were deployed in two field sites (lake and marina), two standard ecotoxicity media, and in primary sewage sludge for a period of up to 48 h. The chemical and physical transformations of AgNPs after exposure to different environments were analyzed by a combination of XAS and SEM/EDX, taken directly from the substrates. Cystine- or glutathione-bound Ag were found to be the dominant forms of Ag in transformed ENMs, but different extents of transformation were observed across different exposure conditions and surface charges. These results successfully demonstrate the feasibility of using immobilized ENMs to examine their likely transformations in situ in real environments and provide further insight into the short-term fate of AgNPs in the environment. Both the advantages and the limitations of this approach are discussed. © 2013 American Chemical Society

Chemical characterisation, antibacterial activity, and (nano)silver transformation of commercial personal care products exposed to household greywater

The objective of this study was to test the original speciation of silver (Ag) in eight different commercially available personal care products and investigate the chemical transformation of Ag during exposure to two types of synthetic greywater. The antimicrobial activity of the products was examined to determine the relationship between Ag content and speciation with the antibacterial functionality of the products. The Ag content of each product was quantified and X-ray absorption near-edge structure (XANES) analysis was used to investigate the initial speciation in the products and the changes occurring upon mixture with greywater. The results showed that the total Ag concentration in the products ranged from 17 to 30 mg kg-1, and was usually below the value reported on the label. Analyses revealed the complexity of Ag speciation in these products and highlighted the importance of characterisation studies to help elucidate the potential risks of nano-Ag in the environment. The antibacterial results confirmed that the antibacterial efficacy of the products depends on the concentration, form and speciation of Ag in the products, but is also significantly affected by product formulation. For instance, many of the products contained additional bactericidal ingredients, making it difficult to determine how much of the bactericidal effect was due directly to the Ag content/species. This paper offers some suggestions for standard methodologies to facilitate cross-comparison of potential risks across different studies and nano-enabled products. This journal is © The Royal Society of Chemistry

Geological controls on overpressure in the Northern Carnarvon Basin

A small, but significant fraction of wells drilled in the Northern Carnarvon Basin have encountered problems with overpressure: better pore pressure prediction would improve safety and economy for drilling operations. In the Northern Carnarvon Basin the occurrence of overpressure and likely mechanisms are under investigation as part of the Australian Petroleum Cooperative Research Centre (APCRC) Research Program on Pore Pressure Prediction. Previous workers have proposed a number of mechanisms as the main cause of overpressure including undercompaction, hydrocarbon generation, horizontal stress and clay reactions.A preliminary regional study was undertaken incorporating over 400 well completion reports which identified approximately 60 wells with mud weights greater than 1.25 S.G. A subset of these wells was investigated and more reliable but much scarcer pressure indicators such as kicks or direct pressure measurements were examined. Depth-pressure profiles of wells across the region are variable and commonly show pressure compartmentalisation. Using a range of indicators, it was observed that overpressured strata in the Barrow Subbasin:occur over a wide depth range (2,500 to 4,000+ mbsl);occur over a wide stratigraphic range (Late Triassic to Late Cretaceous);are not regionally limited by major structural boundaries;are associated with sequences dominated by finegrained sediments with variable clay mineralogy; and in depositionally, or structurally, isolated sandstones; andmainly to the west of the Barrow and Dampier Subbasins around the Alpha Arch and Rankin Trend, coinciding with thickest Tertiary deposition.Previous published work in the study area has tended to support hydrocarbon generation as the primary cause of overpressure, though more recent publications have emphasised compaction disequilibrium. The log response (DT, RHOB and NPHI) of overpressured clay-rich strata has been investigated to constrain the type of overpressure mechanism. A normal compaction trend has been derived for four stratigraphic groupings; Muderong Shale, Barrow Group, Jurassic and Triassic. All overpressure occurrences were accompanied by an increase in sonic transit time. Not all wells have suitable log data for evaluation, but all stratigraphic groups show some evidence of elevated porosity associated with overpressure consistent with disequillibrium compaction as a dominant mechanism. Overpressures in the Barrow Group in Minden-1 and the Jurassic section within Zeepaard–1 do not have accompanying porosity anomalies suggesting a different overpressure mechanism model is needed.</jats:p