The genetic history of the Southern Arc: a bridge between West Asia and Europe

By sequencing 727 ancient individuals from the Southern Arc (Anatolia and its neighbors in Southeastern Europe and West Asia) over 10,000 years, we contextualize its Chalcolithic period and Bronze Age (about 5000 to 1000 BCE), when extensive gene flow entangled it with the Eurasian steppe. Two streams of migration transmitted Caucasus and Anatolian/Levantine ancestry northward, and the Yamnaya pastoralists, formed on the steppe, then spread southward into the Balkans and across the Caucasus into Armenia, where they left numerous patrilineal descendants. Anatolia was transformed by intra–West Asian gene flow, with negligible impact of the later Yamnaya migrations. This contrasts with all other regions where Indo-European languages were spoken, suggesting that the homeland of the Indo-Anatolian language family was in West Asia, with only secondary dispersals of non-Anatolian Indo-Europeans from the steppe

Integration patterns of the asymmetric components of the skull: A covariational statistical approach on a modern greek sample

Objectives: The integration patterns of the asymmetric components of the skull can be informative, especially in the diagnosis and surgical treatment planning of patients. This research aims to explore the interactions of the asymmetric components between the mandible, the basicranium, and the upper face. Material and methods: The sample comprises 100 skulls of Greek adults and the three-dimensional coordinates of 35 landmarks, extracted from the cranial and mandibular virtual 3D bones, were analyzed using the generalized least-squares Procrustes method. The integration patterns of the asymmetric components of the shapes of the specific structures were evaluated through partial least-squares correlation analysis with a two-blocks approach. Results: When handling the skull regions separately, there is statistically significant integration of the asymmetric components between the mandible and the basicranium as well as between the mandible and the upper face. Furthermore, the mandible and the basicranium exhibit the strongest interaction among the three pairs examined. Conclusions: The asymmetric variation of the skull regions should be taken into consideration for surgical treatment planning. However, comparing our findings with other studies revealed that the utilization of the skull regions in the context of a structure as a whole while running the analysis, as well as different landmark combinations, may lead to different results. Furthermore, there might be population-specificity to certain integration patterns of the asymmetric component of the total shape variation among skull regions. Hence, surgical bone restoration, especially in the mandible, should consider overall skull asymmetry and population-specific data to ensure optimal integration. © 2021 E. Schweizerbart’sche Verlagsbuchhandlung, 70176 Stuttgart, Germany

Regression equations for the estimation of stature and body mass using a Greek documented skeletal collection

Body size is an important variable in bioarchaeological and forensic studies, making the accurate calculation of stature and body mass imperative. Given that anatomical and morphometric approaches offer accurate results but require a particularly good preservation of the skeletal material, whereas mathematical and mechanical methods are more easily applicable but they are largely population-specific, the present paper uses a ‘hybrid’ approach in order to generate regression equations for the prediction of stature and body mass in a modern Greek sample. Specifically, anatomical and morphometric methods were used to calculate the stature and body mass of the individuals and regression equations using the Ordinary Least Squares and Reduced Major Axis methods were generated with long bone lengths and femoral head breadth as predictors. The obtained equations exhibit low random and directional error and perform better than existing equations designed using different samples from the United States, Europe, and the Balkans. Therefore, these equations are more appropriate for modern Greek material. © 2017 Elsevier Gmb

Advances in Osteometric Sorting: Utilizing Diaphyseal CSG Properties for Lower Limb Skeletal Pair-Matching

Pair-matching of bilateral elements is a major component of resolving commingled remains both in forensic and bioarchaeological contexts. This study presents a new method of osteometric pair-matching of the lower limbs which relies on 3D digital models of the femur and tibia bones. The proposed method, which is accompanied by a freely available open-source implementation, automatically computes a number of osteometric variables including cross-sectional geometric properties from an assemblage of left and right bone antimeres and calculates probabilistically the appropriate matching pairs as well as single elements, whose bones antimere is not present in the given assemblage. The method has been extensively tested on a skeletal sample comprising 396 femurs and 422 tibias from the Athens collection. Our results in testing commingled assemblages with no disparity show that the method’s sensitivity is 1 for sorting femurs and 0.997 for sorting tibias, whereas in assemblages with moderate disparity the sensitivity is 0.999 and 0.992 respectively. Our results further indicate that sensitivity is unaffected by the size of the commingled assemblage although the percentage of identified true matching pairs drops as the number of commingled elements increases. This means that all identified antimeres matched to an individual are still very accurately sorted despite not every individual being identified in very large assemblages. The proposed method can facilitate the sorting process of commingled remains both accurately and efficiently, while leaving a very small percentage of unsorted elements that may require further techniques for further individualization. © 2020 American Academy of Forensic Science

Validation study of osteometric techniques for sorting commingled human skeletal remains in archaeological samples

The biological profile of human remains is fundamental for understanding the structure of past societies and their life histories. However, the assessment of the demographics of an assemblage is hindered when dealing with commingled burials. Consequently, methods for sorting commingled skeletons are essential in a bioarchaeological context. The aim of this study was to provide insight into osteometric sorting techniques when dealing with archaeological commingled skeletal remains. For the purposes of this research, Byrd and LeGarde's pair-matching osteometric sorting and regression models, which use measurements created for fragmented bones, were tested on 61 adult individuals exhumed from single burial plots from five different Greek archaeological sites. The results showed that the regression models exhibit much lower rates of true positive results (18.2–67.6% and 4.8–71.7% for the left and right side, respectively) than the pair-matching model (74.1–89.5%), whereas the rates of the false positive results are almost equivalent (approximately 20–35%). Therefore, the application of the pair-matching osteometric sorting model can provide a set of potential matches in an archaeological context. However, it is essential to utilize other techniques as well, in order to verify the results. © 2019 John Wiley & Sons, Ltd

Secular change in adult stature of modern Greeks

Objectives: In Greece, during the late 19th and early 20th century, the rural population adopted a more or less urban lifestyle. Furthermore, the first half of the 20th century finds Greece involved in five major wars, including a civil war, and consequent financial deprivation. This study investigates how the socioeconomic changes in Greece, during this period of time, have affected the stature of its population. Methods: The Athens collection constitutes our sample with 189 adult individuals (104 males and 85 females). Stature was estimated with regression equations and secular change was evaluated by linear regression of stature with respect to the year of birth. Further analysis of our population sample was based on three time periods to explore the correlation between secular change and historical events. Results: From 1879 to 1965, stature increased for both males and females. The subsequent analysis among different periods revealed that the male group exhibited a small although non-significant decline in stature during the years 1912 to 1950, which coincides with the long inter-war period. However, females appear less affected by the consequent deprivation. Conclusions: Our findings are consistent with earlier studies based on a much smaller time span. The negative effect of the economic and nutritional deprivation on stature as a result of warfare is apparent in our sample, at least for males. Furthermore, the positive effect of economic growth on stature is prominent for the entire population in the postwar period. © 2017 Wiley Periodicals, Inc

A novel method for analyzing long bone diaphyseal cross-sectional geometry. A GNU Octave CSG Toolkit

The diaphyseal cross-sectional geometric properties of the humerus, femur and tibia have been extensively used for studying their adaptation to mechanical loading. To date common practices for such studies involve either computed tomography or the latex cast method in conjunction with image analysis for calculating such properties. With the advent of modern laser scanning and photogrammetry technologies in biological anthropology, the computation of the cross-sectional geometric properties directly from 3D models is a viable and sensible alternative. Nevertheless, such method has not been properly implemented as yet. A dedicated toolkit, named long-bone-diaphyseal-CSG-Toolkit, comprising a set of functions for the GNU Octave programming language, is presented here. Offering a robust analytical implementation and an easy to follow application either for a single bone or in batch-processing mode, the toolkit requires minimum user intervention and also provides functionality for graphical representation of the calculated periosteal contours and their respective cross-sectional geometric properties. Finally, the long-bone-diaphyseal-CSG-Toolkit utilizes advanced optimization algorithms, which eliminate intra- and inter-observer error by reliably orienting the cross-sectional contours to a well-defined orientation and close to the bone's true anatomical position, which provides a significant advantage over the latex cast method. © 2019 Elsevier B.V

Identification of skeletal remains from a Mycenaean burial in Kastrouli-Desfina, Greece

The present work details the findings of the anthropological study of the skeletal material unearthed from the Kastrouli archaeological site on July 2016. The skeletal material was significantly deteriorated with most of the bones fragmented beyond a level to yield any information. Despite almost no single bone was found intact, hence no metrics for stature estimation are provided herein, a number of sufficiently preserved femur bones along with numerous teeth were identified and allowed for MNI estimates for this commingled burial. We identified 15 adults along with 2 subadults, an infant and a fetus. Skeletal remains of domesticated animals were also recovered from the same undisturbed context, for which the recovered archaeological artifacts suggest that the tomb was Mycenaean/Late Helladic in date. © 2017 MAA

Landmark based sex discrimination on the crania of archaeological Greek populations. A comparative study based on the cranial sexual dimorphism of a modern Greek population

The estimation of sex is a fundamental step for physical anthropologists. The present study confirms the presence of sexual dimorphism in cranial traits of a modern Greek population and produces sex predicting logistic regression equations, which are subsequently applied on the crania of archaeological Greek populations. This study uses 24 landmarks and 25 traits based on distance and angle measurements. Equations, were formulated for the cranium in whole, as well as, isolated cranial regions and single traits. The application of these equations on the archaeological Greek populations yielded an accuracy of classification over 70% in the sphenoid region, the maxilla and the cranium in whole and for three single traits. Hence, the results suggest that our equations based on the modern Greek population can successfully be used in sex prediction of archaeological Greek populations. © 2017 MAA

Sex determination by three-dimensional geometric morphometrics of the vault and midsagittal curve of the neurocranium in a modern Greek population sample

The aim of this study is to assess sexual dimorphism of adult crania in the vault and midsagittal curve of the vault using three-dimensional geometric morphometric methods. The study sample consisted of 176 crania of known sex (94 males, 82 females) belonging to individuals who lived during the 20th century in Greece. The three-dimensional co-ordinates of 31 ecto-cranial landmarks and 30 semi-landmarks were digitized using a MicroScribe 3DX contact digitizer. Generalized Procrustes analysis (GPA) was used to obtain size and shape variables for statistical analysis. Shape, size and form analyses were carried out by logistic regression and three discriminant function analyses. Results indicate that there are shape differences between sexes. Females in the region of the parietal bones are narrower and the axis forming the frontal and occipital bones is more elongated; the frontal bone is more vertical. Sex-specific shape differences give better classification results in the vault (79%) compared with the midsagittal curve of the neurocranium (68.8%). Size alone yielded better results for cranial vault (82%), while for the midsagittal curve of the vault the result is poorer (68.1%). As anticipated, the classification accuracy improves when both size and shape are combined (89.2% for vault, and 79.4% for midsagittal curve of the vault). These latter findings imply that, in contrast to the midsagittal curve of the neurocranium, the shape of the cranial vault can be used as an indicator of sex in the modern Greek population. © 2016