Spectroscopic detection of brain propylene glycol in neonates: Effects of different pharmaceutical formulations of phenobarbital

Background The first choice for treatment of neonatal convulsions is intravenous phenobarbital, which contains propylene glycol (PG) as a solvent. Although PG is generally considered safe, the dosage can exceed safety thresholds in neonates. High PG levels can cause lactic acidosis. Purpose/Hypothesis To investigate a relationship between brain PG concentration and medication administered to neonates, and to study if a correlation between spectroscopically detected PG and lactate was present. Study Type Retrospective. Population Forty‐one neonates who underwent MRI/MRS. Field Strength/Sequence Short echo time single voxel MRS at 1.5T. Assessment Spectra were quantified. Concentrations of PG were correlated with medication administered, because intravenously administered phenobarbital solutions contained 10, 25, or 50 mg phenobarbital per ml, all containing 350 mg PG per ml. The interval between medication and MRI/MRS was determined. Statistical Tests Chi‐square test, Student's t‐test, Mann–Whitney U‐test and Spearman correlation. Results Eighteen neonates had brain PG >1 mM (median 3.4 mM, maximum 9.5 mM). All 18 neonates with high brain PG and 14 neonates with low brain PG (<1 mM) received phenobarbital as the only source of PG. Nine neonates did not receive any phenobarbital/PG‐containing medication. Neonates with high brain PG more often received 10 mg/ml phenobarbital, resulting in higher PG dose (high vs. low brain PG (median [interquartile range]: 1400 [595] vs. 350 [595] mg/kg, respectively, P < 0.01). In addition, the interval between the last phenobarbital dose and MRI was shorter in the high brain PG group (high vs. low brain PG: 16 [21] vs. 95 [83] hours, respectively, P < 0.001). Within neonates that received phenobarbital, there was no conclusive correlation between spectroscopically detected PG and lactate (Spearman's rho = 0.23, P = 0.10). Data Conclusion These MRS findings may increase awareness of potentially toxic PG concentrations in the neonatal brain due to intravenous phenobarbital administration and its dependence on the phenobarbital formulation used. Level of Evidence:4Technical Efficacy:Stage

Reconciling the Cretaceous breakup and demise of the Phoenix Plate with East Gondwana orogenesis in New Zealand

Following hundreds of millions of years of subduction in all circum-Pacific margins, the Pacific Plate started to share a mid-ocean ridge connection with continental Antarctica during a Late Cretaceous south Pacific plate reorganization. This reorganization was associated with the cessation of subduction of the remnants of the Phoenix Plate along the Zealandia margin of East Gondwana, but estimates for the age of this cessation from global plate reconstructions (∼86 Ma) are significantly younger than those based on overriding plate geological records (105–100 Ma). To find where this discrepancy comes from, we first evaluate whether incorporating the latest available marine magnetic anomaly interpretations change the plate kinematic estimate for the end of convergence. We then identify ways to reconcile the outcome of the reconstruction with geological records of subduction along the Gondwana margin of New Zealand and New Caledonia. We focus on the plate kinematic evolution of the Phoenix Plate from 150 Ma onward, from its original spreading relative to the Pacific Plate, through its break-up during emplacement of the Ontong Java Nui Large Igneous Province into four plates (Manihiki, Hikurangi, Chasca, and Aluk), through to the end of their subduction below East Gondwana, to today. Our updated reconstruction is in line with previous compilations in demonstrating that as much as 800–1100 km of convergence occurred between the Pacific Plate and Zealandia after 100 Ma, which was accommodated until 90–85 Ma. Even more convergence occurred at the New Zealand sector owing to spreading of the Hikurangi Plate relative to the Pacific Plate at the Osbourn Trough, with the most recent age constraints suggesting that spreading may have continued until 79 Ma. The end of subduction below most of East Gondwana coincides with a change in relative plate motion between the Pacific Plate and East Gondwana from westerly to northerly, of which the cause remains unknown. In addition, the arrival of the Hikurangi Plateau in the subduction zone occurred independent from, and did not likely cause, the change in Pacific Plate motion. Finally, our plate reconstruction suggests that the previously identified geochemical change in the New Zealand arc around 105–100 Ma that was considered evidence of subduction cessation, may have been caused by Aluk-Hikurangi ridge subduction instead. The final stages of convergence before subduction cessation must have been accommodated by subduction without or with less accretion. This is common in oceanic subduction zones but makes dating the cessation of subduction from geological records alone challenging

Effect of a Coadsorbent on the Performance of Dye-Sensitized TiO2 Solar Cells: Shielding versus Band-Edge Movement

The objective of this research is to determine the operational characteristics key to efficient, low-cost, stable solar cells based on dye-sensitized mesoporous films (in collaboration with DOE's Office of Science Program). Toward this end, we have investigated the mechanism by which the adsorbent chenodeoxycholate, cografted with a sensitizer onto TiO2 nanocrystals, improves the open-circuit photovoltage (VOC) and short-circuit photocurrent density (JSC). We find that adding chenodeoxycholate not only shifts the TiO2 conduction-band edge to negative potentials but also accelerates the rate of recombination. The net effect of these opposing phenomena is to produce a higher photovoltage. It is also found that chenodeoxycholate reduces the dye loading significantly but has only a modest effect on JSC. Implications of these results to developing more efficient cells are discussed

Control of quantum dot emission by colloidal plasmonic pyramids in a liquid crystal

We study the plasmon-enhanced fluorescence of a single semiconducting quantum dot near the apex of a colloidal gold pyramid spatially localized by the elastic forces of the liquid crystal host. The gold pyramid particles were manipulated within the liquid crystal medium by laser tweezers, enabling the self-assembly of a semiconducting quantum dot dispersed in the medium near the apex of the gold pyramid, allowing us to probe the plasmon-exciton interactions. We demonstrate the effect of plasmon coupling on the fluorescence lifetime and the blinking properties of the quantum dot. Our results demonstrate that topological defects around colloidal particles in liquid crystal combined with laser tweezers provide a platform for plasmon exciton interaction studies and potentially could be extended to the scale of composite materials for nanophotonic applications.</p

The Effect of Transposable Element Insertions on Gene Expression Evolution in Rodents

Background:Many genomes contain a substantial number of transposable elements (TEs), a few of which are known to be involved in regulating gene expression. However, recent observations suggest that TEs may have played a very important role in the evolution of gene expression because many conserved non-genic sequences, some of which are know to be involved in gene regulation, resemble TEs. Results:Here we investigate whether new TE insertions affect gene expression profiles by testing whether gene expression divergence between mouse and rat is correlated to the numbers of new transposable elements inserted near genes. We show that expression divergence is significantly correlated to the number of new LTR and SINE elements, but not to the numbers of LINEs. We also show that expression divergence is not significantly correlated to the numbers of ancestral TEs in most cases, which suggests that the correlations between expression divergence and the numbers of new TEs are causal in nature. We quantify the effect and estimate that TE insertion has accounted for ~20% (95% confidence interval: 12% to 26%) of all expression profile divergence in rodents. Conclusions:We conclude that TE insertions may have had a major impact on the evolution of gene expression levels in rodents

MouseIndelDB: a database integrating genomic indel polymorphisms that distinguish mouse strains

MouseIndelDB is an integrated database resource containing thousands of previously unreported mouse genomic indel (insertion and deletion) polymorphisms ranging from ∼100 nt to 10 Kb in size. The database currently includes polymorphisms identified from our alignment of 26 million whole-genome shotgun sequence traces from four laboratory mouse strains mapped against the reference C57BL/6J genome using GMAP. They can be queried on a local level by chromosomal coordinates, nearby gene names or other genomic feature identifiers, or in bulk format using categories including mouse strain(s), class of polymorphism(s) and chromosome number. The results of such queries are presented either as a custom track on the UCSC mouse genome browser or in tabular format. We anticipate that the MouseIndelDB database will be widely useful for research in mammalian genetics, genomics, and evolutionary biology. Access to the MouseIndelDB database is freely available at: http://variation.osu.edu/

In situ interface engineering for probing the limit of quantum dot photovoltaic devices.

Quantum dot (QD) photovoltaic devices are attractive for their low-cost synthesis, tunable band gap and potentially high power conversion efficiency (PCE). However, the experimentally achieved efficiency to date remains far from ideal. Here, we report an in-situ fabrication and investigation of single TiO2-nanowire/CdSe-QD heterojunction solar cell (QDHSC) using a custom-designed photoelectric transmission electron microscope (TEM) holder. A mobile counter electrode is used to precisely tune the interface area for in situ photoelectrical measurements, which reveals a strong interface area dependent PCE. Theoretical simulations show that the simplified single nanowire solar cell structure can minimize the interface area and associated charge scattering to enable an efficient charge collection. Additionally, the optical antenna effect of nanowire-based QDHSCs can further enhance the absorption and boost the PCE. This study establishes a robust 'nanolab' platform in a TEM for in situ photoelectrical studies and provides valuable insight into the interfacial effects in nanoscale solar cells