Use of recurrence quantification analysis to examine associations between changes in text structure across an expressive writing intervention and reductions in distress symptoms in women wth breast cancer

The current study presents an exploratory analysis of using Recurrence Quantification Analysis (RQA) to analyze text data from an Expressive Writing Intervention (EWI) for Danish women treated for Breast Cancer. The analyses are based on the analysis of essays from a subsample with the average age 54.6 years (SD = 9.0), who completed questionnaires for cancer-related distress (IES) and depression symptoms (BDI-SF). The results show a significant association between an increase in recurrent patterns of text structure from first to last writing session and a decrease in cancer-related distress at 3 months post-intervention. Furthermore, the change in structure from first to last essay displayed a moderate, but significant correlation with change in cancer-related distress from baseline to 9 months post-intervention. The results suggest that changes in recurrence patterns of text structure might be an indicator of cognitive restructuring that leads to amelioration of cancer-specific distress

Using measures of reading time regularity (RTR) to quantify eye movement dynamics, and how they are shaped by linguistic information

In this article, we present the concept of reading time regularity (RTR) as a measure to capture reading process dynamics. The first study is concerned with examining one of the assumptions of RTR, namely, that process measures of reading, such as eye movement fluctuations and fixation durations, exhibit higher regularity when contingent on sequentially structured information, such as texts. To test this, eye movements of 26 German native speakers were recorded during reading-unrelated and reading-related tasks. To analyze the data, we used recurrence quantification analysis and sample entropy analysis to quantify the degree of temporal structure in time series of gaze steps and fixation durations. The results showed that eye movements become more regular in reading compared to nonreading conditions. These effects were most prominent when calculated on the basis of gaze step data. In a second study, eye movements of 27 native speakers of German were recorded for five conditions with increasing linguistic information. The results replicate the findings of the first study, verifying that these effects are not due to mere differences in task instructions between conditions. Implications for the concept of RTR and for future studies using these metrics in reading research are discussed

Unidimensional and Multidimensional Methods for Recurrence Quantification Analysis with crqa

Recurrence quantification analysis is a widely used method for characterizing patterns in time series. This article presents a comprehensive survey for conducting a wide range of recurrence based analyses to quantify the dynamical structure of single and multivariate time series and capture coupling properties underlying leader-follower relationships. The basics of recurrence quantification analysis (RQA) and all its variants are formally introduced step-by-step from the simplest auto recurrence to the most advanced multivariate case. Importantly, we show how such RQA methods can be deployed under a single computational framework in R using a substantially renewed version of our crqa 2.0 package. This package includes implementations of several recent advances in recurrence based analysis, among them applications to multivariate data and improved entropy calculations for categorical data. We show concrete applications of our package to example data, together with a detailed description of its functions and some guidelines on their usage

Global temporal typing patterns in foreign language writing: Exploring language proficiency through recurrence quantification analysis (RQA)

Recurrence quantification analysis (RQA) is a time-series analysis method that uses autocorrelation properties of typing data to detect regularities within the writing process. The following paper first gives a detailed introduction to RQA and its application to time series data. We then apply RQA to keystroke logging data of first and foreign language writing to illustrate how outcome measures of RQA can be understood as skill-driven constraints on keyboard typing performance. Forty native German students performed two prompted writing assignments, one in German and one in English, a standardized copy task, and a standardized English placement test. We assumed more fluent and skilled writing to reveal more structured typing time series patterns. Accordingly, we expected writing in a well-mastered first language to coincide with higher values in relevant RQA measures as compared to writing in a foreign language. Results of mixed model ANOVAs confirmed our hypothesis. We further observed that RQA measures tend to be higher, thus indicating more structured data, whenever parameters of pause, burst, and revision analyses indicate more fluent writing. Multiple regression analyses revealed that, in addition to typing skills, language proficiency significantly predicts outcomes of RQA. Thus, the present data emphasize RQA being a valuable resource for studying time series data that yields meaningful information about the effort a writer must exert during text production

Unidimensional and Multidimensional Methods for Recurrence Quantification Analysis with crqa

Recurrence quantification analysis is a widely used method for characterizing patterns in time series. This article presents a comprehensive survey for conducting a wide range of recurrence-based analyses to quantify the dynamical structure of single and multivariate time series, and to capture coupling properties underlying leader-follower relationships. The basics of recurrence quantification analysis (RQA) and all its variants are formally introduced step-by-step from the simplest autorecurrence to the most advanced multivariate case. Importantly, we show how such RQA methods can be deployed under a single computational framework in R using a substantially renewed version our crqa 2.0 package. This package includes implementations of several recent advances in recurrence-based analysis, among them applications to multivariate data, and improved entropy calculations for categorical data. We show concrete applications of our package to example data, together with a detailed description of its functions and some guidelines on their usage

The mycotoxin phomoxanthone A disturbs the form and function of the inner mitochondrial membrane.

Mitochondria are cellular organelles with crucial functions in the generation and distribution of ATP, the buffering of cytosolic Ca2+ and the initiation of apoptosis. Compounds that interfere with these functions are termed mitochondrial toxins, many of which are derived from microbes, such as antimycin A, oligomycin A, and ionomycin. Here, we identify the mycotoxin phomoxanthone A (PXA), derived from the endophytic fungus Phomopsis longicolla, as a mitochondrial toxin. We show that PXA elicits a strong release of Ca2+ from the mitochondria but not from the ER. In addition, PXA depolarises the mitochondria similarly to protonophoric uncouplers such as CCCP, yet unlike these, it does not increase but rather inhibits cellular respiration and electron transport chain activity. The respiration-dependent mitochondrial network structure rapidly collapses into fragments upon PXA treatment. Surprisingly, this fragmentation is independent from the canonical mitochondrial fission and fusion mediators DRP1 and OPA1, and exclusively affects the inner mitochondrial membrane, leading to cristae disruption, release of pro-apoptotic proteins, and apoptosis. Taken together, our results suggest that PXA is a mitochondrial toxin with a novel mode of action that might prove a useful tool for the study of mitochondrial ion homoeostasis and membrane dynamics