10 research outputs found
Chemokine-induced secretion of gelatinase B in primary human monocytes
Chemokines help control normal leukocyte trafficking as well as their infiltration into tissues during acute and chronic inflammation. Matrix metalloproteinases (MMPs) help support the extravasation and infiltration of leukocytes through limited proteolysis of basement membranes and matrix material. The effect of the chemokines RANTES/CCL5, MCP-1/CCL and SDF-1 /CXCL12 on secretion of the matrix metalloproteinase B and its endogenous inhibitor TIMP-1 was studied. RANTES/CCL5 and SDF-1/CXCL12 were found to induce MMP-9 secretion in primary human monocytes while TIMP-1 secretion was not affected. RANTES/CCL5 effects were mediated through CCR1 because the CCR1 antagonist BX471 was found to effectively block RANTES/CCL5-induced MMP-9 secretion
Jacalin, another marker for histiocytes in paraffin-embedded tissues
Jacalin is a lectin which reacts with Dgalactose.
We have tested jacalin on 75 samples of
different formalin- and alcohol-fixed tissues. A
consistent cytoplasmic stain of the histiocytes was
observed in paraffin-embedded tissues in al1 cases
studied of reactive sinus histiocytosis, macrophages in
clear centres of follicular hyperplasia, in tuberculosis
granulomas and in osteoclast-like giant cells in a breast
carcinoma. We failed to find any clear binding of jacalin
to the cells of eosinophilic granulomas, giant cell tumors
of tendon sheath, pleomorphic malignant fibrous
histiocytomas, Hodgkin's disease, melanomas, nevi or
signet ring cell carcinomas of the breast and stomach. It
seems that jacalin is a good marker for free histiocytesl
macrophages, not for fixed histiocytes and tumors
related to them. This lectin might play a role in
differential diagnosis with histiocyte mimicking
processes