Unsere Pflanzenwelt im Umbruch

In unserer Zeit jagen sich die Hiobsbotschaften über die Verarmung der Umwelt. Ist es möglich, rasch eine ausgewogene Übersicht der wirklichen Lage zu gewinnen? Für die höheren Pflanzen des Kreises Höxter östlich vom 9.Meridian seit 1976 ja! Damals erschien der "Atlas zur Flora von Südniedersachsen" von H. HAEUPLER. Er umfaßt zu 96% auch die Fläche des Kreises Höxter. Im Gegensatz zu den geschriebenen Floren, die nur bei den selteneren Arten Verbreitungsangaben bringen, arbeitet der Atlas bei allen Arten gleichmäßig flächendeckend. Das ganze Gebiet ist in Grundfelder eingeteilt, hier in Viertel der "Topographischen Karte 1: 25.000", die früher "Meßtischblatt" hieß. In jedem dieser "MTB-Quadranten" hakt mindestens ein Bearbeiter alle Pflanzenarten, die er dort findet, in einer vorgedruckten Liste an. Jede solche Feststellung erscheint in der Karte als Punkt, ältere Angaben, die nach 1945 nicht mehr bestätigt werden konnten, als Kreis

Mechanics of the Ski-Snow Contact

Two outstanding questions of the ski-snow friction are considered: the deformation mode of the snow and the real contact area. The deformation of hard, well sintered snow in a short time impact has been measured with a special linear friction tester. Four types of deformations have been identified: brittle fracture of bonds, plastic deformation of ice at the contact spots, elastic and delayed elastic deformation of the snow matrix. The latter is the dominant deformation in the ski-snow contact. Based on the measured loading curves the mechanical energy dissipation of snow deformation in skiing on hard snow has been determined and found negligible compared to the thermal energy dissipation. A mechanical model consisting of ice spheres supported by rheological elements (a non-linear spring in series with a Kelvin element) is proposed to model the deformation of snow in the ski-snow contact. The model can describe the delayed elastic behaviour of snow. Coupled with the complete topographical description of the snow surface obtained from X-ray micro computer tomography measurements, the model predicts the number and area of contact spots between ski and snow. An average contact spot size of 110μm, and a relative real contact area of 0.4% has been foun

Modeling of Bearing Voltage in Electric Machines Based on Electromagnetic FEA and Measured Bearing Capacitance

Bearing voltages and associated bearing currents in electric machines driven by pulsewidth modulation converters with high switching frequencies and high dv/dt can cause premature bearing failures. This article proposes a new modeling approach for the prediction of steady-state and transient bearing voltages based on two-dimensional (2-D) electromagnetic finite element analysis with coupled external circuits using measured bearing capacitance values. The distributed-element external circuit was employed mainly to take into account the influence of wire distribution and frequency dependency, which are typically neglected by traditional equivalent circuits. The developed model was then used to simulate bearing voltages for various scenarios and evaluate the effectiveness of several easy-to-implement bearing voltage reduction methods from the perspective of machine design and manufacturing, such as using the insulated shaft and/or bearings, introducing additional insulation in the rotor, and changing the material of machine components. Experimental measurements are also provided to facilitate the analysis and validate the proposed approach

Evaluation of Bearing Voltage Reduction in Electric Machines by Using Insulated Shaft and Bearings

Bearing voltages and corresponding currents in electric machines driven by pulse width modulation (PWM) converters with fast switching and high dv/dt can cause premature bearing failures. This paper evaluates the bearing voltage reduction by using insulated shafts and bearings. An equivalent circuit representation of electric machines taking into account high-frequency effects is developed to show the production mechanism of bearing voltage, based on which simulations are performed with detailed finite element models for transient and simplified equivalent circuit for steady-state analysis. The steady-state equivalent circuit is then calibrated, by combined numerical calculations and experimental measurements, and used to predict the bearing voltage for various scenarios, proving the effectiveness of using insulated shaft and bearings in reducing the steady-state bearing voltage

Combined Numerical and Experimental Determination of Ball Bearing Capacitances for Bearing Current Prediction

High-frequency voltages across the steel ball bearings and the corresponding currents can cause premature bearing failures in electric machines driven by PWM converters. The bearing voltage, one of the most commonly-used failure indicators, depends heavily on the bearing capacitance. This paper presents a combined numerical and experimental approach for the calculation of ball bearing capacitances to address the uncertainty introduced by lubricant property, lubrication status and other metal parts, such as seals and ball retainers. Based on the obtained capacitance breakdown, the influences of temperature, speed and bearing load (radial, axial or combined) on the capacitance are studied. Measurements and associated results of bearing capacitances are provided to validate the proposed method

A Systematic Study on the Effects of Dimensional and Materials Tolerances on Permanent Magnet Synchronous Machines Based on the IEEE Std 1812

In the process of designing and manufacturing an electrical machine, a systematic study of dimensional and material tolerances is of the utmost importance. This paper proposes a systematic method by which the effect of design specification variations on permanent magnet (PM) synchronous machine performance may be identified and quantified. The method combines design of experiments techniques, open-circuit and short-circuit physical measurements, and virtual test simulations conducted based on the recently approved IEEE Std 1812 testing guide. Three case studies, two provided by a spoke-type PM radial field machine configuration, in two designs with different electromagnetic loading, and an axial flux PM machine are discussed. It is shown that based on the output performance, out of specification tolerances for magnet remanence, steel grade, as well as dimensional variables, and stator to rotor eccentricity, may be identified under certain conditions. It is also exemplified that the ratings, magnetic loading, and configuration of the machine play critical roles and should be thoroughly considered as part of the studies

siRNA carrying an (E)-vinylphosphonate moiety at the 5' end of the guide strand augments gene silencing by enhanced binding to human Argonaute-2

Efficient gene silencing by RNA interference (RNAi) in vivo requires the recognition and binding of the 5'- phosphate of the guide strand of an siRNA by the Argonaute protein. However, for exogenous siRNAs it is limited by the rapid removal of the 5'- phosphate of the guide strand by metabolic enzymes. Here, we have determined the crystal structure of human Argonaute-2 in complex with the metabolically stable 5'-(E)-vinylphosphonate (5'-E-VP) guide RNA at 2.5-A resolution. The structure demonstrates how the 5' binding site in the Mid domain of human Argonaute-2 is able to adjust the key residues in the 5'-nucleotide binding pocket to compensate for the change introduced by the modified nucleotide. This observation also explains improved binding affinity of the 5'-E-VP -modified siRNA to human Argonaute-2 in-vitro, as well as the enhanced silencing in the context of the trivalent N-acetylgalactosamine (GalNAc)-conjugated siRNA in mice relative to the un-modified siRNA

Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions

Methods for site-specific modification of proteins should be quantitative and versatile with respect to the nature and size of the biological or chemical targets involved. They should require minimal modification of the target, and the underlying reactions should be completed in a reasonable amount of time under physiological conditions. Sortase-mediated transpeptidation reactions meet these criteria and are compatible with other labeling methods. Here we describe the expression and purification conditions for two sortase A enzymes that have different recognition sequences. We also provide a protocol that allows the functionalization of any given protein at its C terminus, or, for select proteins, at an internal site. The target protein is engineered with a sortase-recognition motif (LPXTG) at the place where modification is desired. Upon recognition, sortase cleaves the protein between the threonine and glycine residues, facilitating the attachment of an exogenously added oligoglycine peptide modified with the functional group of choice (e.g., fluorophore, biotin, protein or lipid). Expression and purification of sortase takes ∼3 d, and sortase-mediated reactions take only a few minutes, but reaction times can be extended to increase yields.National Institutes of Health (U.S.) (Grant RO1 AI08787

Site-Specific Chemoenzymatic Labeling of Aerolysin Enables the Identification of New Aerolysin Receptors

Aerolysin is a secreted bacterial toxin that perforates the plasma membrane of a target cell with lethal consequences. Previously explored native and epitope-tagged forms of the toxin do not allow site-specific modification of the mature toxin with a probe of choice. We explore sortase-mediated transpeptidation reactions (sortagging) to install fluorophores and biotin at three distinct sites in aerolysin, without impairing binding of the toxin to the cell membrane and with minimal impact on toxicity. Using a version of aerolysin labeled with different fluorophores at two distinct sites we followed the fate of the C-terminal peptide independently from the N-terminal part of the toxin, and show its loss in the course of intoxication. Making use of the biotinylated version of aerolysin, we identify mesothelin, urokinase plasminogen activator surface receptor (uPAR, CD87), glypican-1, and CD59 glycoprotein as aerolysin receptors, all predicted or known to be modified with a glycosylphosphatidylinositol anchor. The sortase-mediated reactions reported here can be readily extended to other pore forming proteins.National Institutes of Health (U.S.) (grant R01 AI087879