Participatory Technology and Constraints Assessment to Improve the Livelihood of Beekeepers in Tigray Region, northern Ethiopia

Beekeeping is a long-standing practice in the rural communities of Ethiopia and appears as ancient history of the country. A three–part assessment and diagnostic study (Livelihood systems assessment, integrated honeybee management needs assessment and diagnostic survey) was undertaken from 2006-2008 in Tigray, Ethiopia to identify market and technological constraints facing the honey sub sector and asses bee pests and the control measures taken. Information was gathered through PRA, interview with key informants and integrated honeybee management diagnostic survey in the rural areas. The assessment and diagnostic activities were undertaken in six zones (Western, North western, Central, Eastern, Southern and Southeastern zones of the region). One hundred nineteen beekeepers were randomly selected. Information on the adoption of new technology, availability of honeybee pests, average annual harvest of honey, honeybee pest controlling measures, market and technological constraints etc. were sought from the beekeepers. Honey yield was markedly different for the traditional and modern hives. On average, it was about 8-15 kg/hive and 20-30 kg/hive from the traditional and modern hives respectively. Lack of adequate bee forages, poor market, lack of trained development agents, inadequate government support, bee pests and inadequate training are mainly the problems facing the honey sub sector in the region. There are different kinds of bee pests and predators. Honey is harvested twice a year. From this study it was realized that almost all beekeeping practices are traditional except little intervention with improved beekeeping practices. Key words: Beekeeping, Bee management, Beehive, Tigray

Intestinal Helminthes Infections and Re-Infections with Special Emphasis on Schistosomiasis Mansoni in Waja, North Ethiopia

To determine the status of infection and re-infection caused by Schistosoma mansoni in a small town of Waja, northern Ethiopia, stool samples were collected from primary school children in two rounds (in mid June and mid September 2004) and were examined using the Kato thick smear method. In addition, water bodies that might serve as biotopes for the intermediate host snails were located and searched using scoops. During the first survey, the prevalence of S. mansoni among the 224 children (119 male and 105 females) sampled was 27.1%. S. mansoni prevalence increased from 27.1% to 36.4% (P< 0.05), during the 2nd survey, three months following the treatment of the positive cases. Similarly, an increase in the prevalence of T. trichiura was observed (from 16 to 30.7%), whereas that of Ascaris lumbricoides decreased during the second survey (from 50 to 42.8%) (

Environmental and Social Sustainability of Urban And Periurban Agriculture (UPA) In Selected Towns Of Ethiopia

Today nearly a billion of world population makes their daily bread from UPA, the sector that has received only little attention. This paper tries to assess the sustainability of urban agriculture from social and environmental point of view. The paper used a combination of primary and secondary data.  The primary data is collected through questionnaires and structured interview specially designed for this study. About 280 households are selected using proportionate-stratified sampling techniques, the stratification being the size of urban farmers in each of the town. The study shows that the environmental and health aspect of sustainability is closer to the yellow line mainly due to extensive use of chemical inputs. From social and political point of view, UPA is inclined to be unsustainable. Thus, city municipalities and national policy makers have provide the necessary support and grant legal recognition that matches the contribution of UPA to the national economic development. Key words: sustainability, urban and peri-urban, agriculture, Ethiopi

Plasma Soluble Human Elastin Fragments as an Intra-Aneurysmal Localized Biomarker for Ruptured Intracranial Aneurysm

Background—Fragmentation of the tunica media is a hallmark of intracranial aneurysm formation, often leading to aneurysmal progression and subsequent rupture. The objective of this study is to determine the plasma level of elastin fragments in the lumen of ruptured versus unruptured human intracranial aneurysms. Methods and Results—One hundred consecutive patients with/without ruptured saccular intracranial aneurysms undergoing endovascular coiling or stent-assisted coiling were recruited. Blood samples were collected from the lumen of intracranial aneurysm using a microcatheter. The tip of the microcatheter was placed inside the aneurysm’s sac in close proximity to the inner wall of the dome. Plasma levels of elastin fragments were measured using an ELISA-based method. Mean plasma level of soluble human elastin fragments was significantly greater in ruptured aneurysms when compared with nonruptured aneurysms (102.0±15.5 versus 39.3±9.6 ng/mL; P\u3c0.001). Mean plasma level of soluble human elastin fragments did not have significant correlation with age, sex, size, or aneurysm location. Conclusions—The present study revealed that a significantly higher concentration of soluble human elastin fragments in the lumen of ruptured intracranial aneurysms when compared with nonruptured ones. © 2018 The Authors

Treatment outcome of children with severe acute malnutrition admitted to therapeutic feeding centers in Southern Region of Ethiopia

Background: Inadequate intake of carbohydrates, proteins, vitamins and minerals is one of the causes for malnutrition. It often affects young children and contributes to more than 60% of deaths in children in developing countries. One in four of malnourished children receiving traditional treatment die during or soon after treatment. The study aimed to assess the treatment outcome of children treated in therapeutic feeding centers (TFC) in southern Ethiopia.Methods: A retrospective review of reports submitted by the TFCs was done during 2003-2004. Data was collected from the monthly reports using standardized formats. The variables included age, treatment centers, type of malnutrition and treatment outcome which were analyzed using descriptive statistics.Results: Of 11,335 cases of malnutrition, 47% (5447) had severe wasting and 53% (6103) had edematous malnutrition. Of the total, 87% (11,191) were cured while 3.6% (468) had died. The average length of stay was 25 and 21 days with an average weight gain of 14 and 13.4 g/kg/d for children with severe wasting and edematous malnutrition, respectively. With increasing age, the death rate decrease  and cure rate increased (p < 0.05 for both).Discussion: Our results show that the new management approach  implemented in the TFC improved the treatment outcome of malnourished children compared to the minimum international standard set for  management of severe acute malnutrition which is cure rate of at least 75% and death rate less than 10%, average length of stay of less than30 days and average weight gain of 8g/kg/day

The Genetics of Adverse Drug Outcomes in Type 2 Diabetes:A Systematic Review

Background: Adverse drug reactions (ADR) are a major clinical problem accounting for significant hospital admission rates, morbidity, mortality, and health care costs. One-third of people with diabetes experience at least one ADR. However, there is notable interindividual heterogeneity resulting in patient harm and unnecessary medical costs. Genomics is at the forefront of research to understand interindividual variability, and there are many genotype-drug response associations in diabetes with inconsistent findings. Here, we conducted a systematic review to comprehensively examine and synthesize the effect of genetic polymorphisms on the incidence of ADRs of oral glucose-lowering drugs in people with type 2 diabetes. Methods: A literature search was made to identify articles that included specific results of research on genetic polymorphism and adverse effects associated with oral glucose-lowering drugs. The electronic search was carried out on 3rd October 2020, through Cochrane Library, PubMed, and Web of Science using keywords and MeSH terms. Result: Eighteen articles consisting of 10, 383 subjects were included in this review. Carriers of reduced-function alleles of organic cation transporter 1 (OCT 1, encoded by SLC22A1) or reduced expression alleles of plasma membrane monoamine transporter (PMAT, encoded by SLC29A4) or serotonin transporter (SERT, encoded by SLC6A4) were associated with increased incidence of metformin-related gastrointestinal (GI) adverse effects. These effects were shown to exacerbate by concomitant treatment with gut transporter inhibiting drugs. The CYP2C9 alleles, (*)2 (rs1799853C>T) and (*)3 (rs1057910A>C) that are predictive of low enzyme activity were more common in subjects who experienced hypoglycemia after treatment with sulfonylureas. However, there was no significant association between sulfonylurea-related hypoglycemia and genetic variants in the ATP-binding cassette transporter sub-family C member 8 (ABCC8)/Potassium Inwardly Rectifying Channel Subfamily J Member 11 (KCNJ11). Compared to the wild type, the low enzyme activity C allele at CYP2C8(*)3 (rs1057910A>C) was associated with less weight gain whereas the C allele at rs6123045 in the NFATC2 gene was significantly associated with edema from rosiglitazone treatment. Conclusion: In spite of limited studies investigating genetics and ADR in diabetes, some convincing results are emerging. Genetic variants in genes encoding drug transporters and metabolizing enzymes are implicated in metformin-related GI adverse effects, and sulfonylurea-induced hypoglycemia, respectively. Further studies to investigate newer antidiabetic drugs such as DPP-4i, GLP-1RA, and SGLT2i are warranted. In addition, pharmacogenetic studies that account for race and ethnic differences are required

Key stakeholders and actions to address Lake Beseka’s challenges in Ethiopia: a social network approach

Lake Beseka is a shallow, saline, endorheic lake in the East African Rift Valley of Ethiopia that has dramatically grown in size due to large-scale irrigation development in its catchment area. Recent artificial connections of the lake with the Awash River system to contain lake size have led to a series of changes and impacts on different water users, but are not reflected in lake and Awash River governance and institutions. Understanding who are the key actors affecting Lake Beseka and strengthening their linkages can help identify solutions that sustainably contain or reduce the lake’s size, improve its water quality, and address costs to nearby and downstream populations as well as the environment. Thus, this study analyzed qualitative data collected from net-mapping – a network analysis that identifies actors or stakeholders as well as linkages and relative power positions among stakeholders. The resulting network reflects the complexity of the water governance system including upstream actors who affect the size and quality of the lake as well as downstream actors who suffer from adverse consequences. The Awash Basin Development Authority, Metehara Sugar Factory, regional bureaus, and federal ministries were identified as the most influential actors affecting how Lake Beseka is used and managed. Actors most affected by the lake expansion and quality problems such as downstream communities currently have no role in the governance of the lake. Metehara Municipality, woreda offices, research institutes, and farmers were considered to have moderate influence. Stakeholders who participated in the net-mapping workshops identified flooding, salinity, water-related conflict, and health effects as the four main challenges of the lake. The study suggests that developing multi-stakeholder partnerships or platforms across most influential and most affected actors could support a more comprehensive understanding of the multiple challenges Lake Beseka is posing. It could also foster the development of more integrated solutions that support the different stakeholders in the lake catchment area and the Awash River Basin

Light-Induced Charge Separation in Photosystem I from Different Biological Species Characterized by Multifrequency Electron Paramagnetic Resonance Spectroscopy

Photosystem I (PSI) serves as a model system for studying fundamental processes such as electron transfer (ET) and energy conversion, which are not only central to photosynthesis but also have broader implications for bioenergy production and biomimetic device design. In this study, we employed electron paramagnetic resonance (EPR) spectroscopy to investigate key light-induced charge separation steps in PSI isolated from several green algal and cyanobacterial species. Following photoexcitation, rapid sequential ET occurs through either of two quasi-symmetric branches of donor/acceptor cofactors embedded within the protein core, termed the A and B branches. Using high-frequency (130 GHz) time-resolved EPR (TR-EPR) and deuteration techniques to enhance spectral resolution, we observed that at low temperatures prokaryotic PSI exhibits reversible ET in the A branch and irreversible ET in the B branch, while PSI from eukaryotic counterparts displays either reversible ET in both branches or exclusively in the B branch. Furthermore, we observed a notable correlation between low-temperature charge separation to the terminal [4Fe-4S] clusters of PSI, termed FA and FB, as reflected in the measured FA/FB ratio. These findings enhance our understanding of the mechanistic diversity of PSI's ET across different species and underscore the importance of experimental design in resolving these differences. Though further research is necessary to elucidate the underlying mechanisms and the evolutionary significance of these variations in PSI charge separation, this study sets the stage for future investigations into the complex interplay between protein structure, ET pathways, and the environmental adaptations of photosynthetic organisms