ANALISIS DAMPAK VARIABEL VARIABEL MARKETING MIX DALAM MEMBANGUN KEPUASAN PELANGGAN TOKO MEBEL DINA PARE KEDIRI (Studi Pada Pelanggan Toko Mebel Dina Pare Kediri)

ABSTRACT Competition in today's business world is so tight that as the number of business people increases to meet consumer needs, both goods and services, businesses are increasingly facing challenges to get the market share they want. Meeting the needs and desires of consumers requires a marketing concept called the marketing mix where to apply a good marketing mix is to pay attention to the elements of supply, price, promotion and distribution of selling products. In marketing there is a marketing strategy called the marketing mix or marketing mix which has an important role in influencing consumers to be able to buy a product or service offered by the company. The mix used in this study are products, prices, places and promotions. This research was conducted at the Dina Pare Furniture Shop located on Wr. Supratman No.35 Pare Kediri. This research was conducted to find out the impact of 4p variables affecting customer satisfaction in the Furniture Shop. From this study it was found that the product mix marketing variable, price, place and promotion affect customer satisfaction. Furniture Dina has good quality products, has a good model, there are many variations and the design of the products sold in the Dina Furniture Shop is interesting. Prices of Furniture in Toko Dina are cheap The price of the product in the Dina Store competes with other stores. Shop Dina has a fixed discount. The shop has a discount every month. The price of products in Dina Stores is affordable. The advertisement owned by the Dina Shop is interesting. Ads owned by Dina Stores according to the product. Dina's store has a brochure ad. Attractive store promotions are attractive sales. Clean storefront The location of Toko Dina is easily accessible to customers. Toko Dina has an outdoor parking lot. Shops are driven by public transportation. Toko Dina is comfortable. And supported by Shop Customer results, Dina is happy, Dina's Shop Customers want to buy again. Customers are willing to buy products at Dina Stores. Customers are willing to recommend. Customers happy or satisfied. Products at Dina Shop are priced accordingly. Keywords: Marketing mix, customer satisfactio

Dot-based ELISA and RIA: Two rapid assays that screen hybridoma supernatants against whole live cells

Two assays are described that are suitable for the screening of large numbers of hybridoma supernatants as well as for the screening of a wide range of tissues for the distribution of a given cell surface antigen. These assays use whole live cells as targets, avoiding fixation or extraction, which could alter the antigenic structural profile. The assays are rapid, simple and inexpensive. Their advantages and applications are discussed.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/24666/1/0000079.pd

Improved production by domain inversion of single-chain Fv antibody fragment against high molecular weight proteoglycan for the radioimmunotargeting of melanoma.

Melanoma is among the few cancers with rising incidence. Currently there is no effective treatment for metastatic disease, but improved detection of melanoma has the potential to benefit the management of patients with early disease. Radioimmunodection by imaging with single-chain Fv (scFv) antibody fragments is one such emerging diagnostic method. However, the amount of scFv that can be produced at a scale suitable for use in patients is limiting. We have previously shown that the bacterial expression of a scFv derived from a monoclonal antibody (MAb) specific for melanoma-associated proteoglycan can be increased by light chain shuffling. In this report we show that a further increase in expression yield can be obtained by reversing the usual V(H)-V(L) orientation of scFvs to V(L)-V(H). Such seemingly minor changes have previously been reported to have unexpected effects on the in vitro and in vivo binding properties of recombinant antibodies. Our results show that reversal of the V domain orientation of the scFv improves expression by 150% without an adverse effect on melanoma binding in vitro and tumor targeting in vivo. Therefore, our results show that alteration of V domain orientation can improve the production yield of clinically useful antibody fragments. When used in combination with other antibody engineering approaches for increased antibody production changing the domain orientation is a simple strategy to achieve significant improvements in the production of scFvs for tumor radioimmunodetection for patient studies

Spatiotemporal dynamics of nicotinic acetylcholine receptors and lipid platforms

Abstract: The relationships between neurotransmitter receptors and their membrane environment are complex, mutual (bidirectional) and physiologically important. Some of these relationships are established with subsets of the membrane lipid population, in the form of lipid platforms, lateral heterogeneities of the bilayer lipid having a dynamic chemical composition distinct from that of the bulk membrane. In addition to the equilibrium between the biosynthetic production, exocytic delivery and recycling of receptors on the one hand, and the endocytic internalization on the other, lateral diffusion, clustering and anchorage of receptors at the lipid platforms play key roles in determining the amount of active receptors at the synapse. Mobile receptors traffic between reservoir non-synaptic membranes and the synapse predominantly by thermally driven Brownian motion, and become immobilized at the perisynaptic region or the synapse proper by various mechanisms. These comprise: (a) clustering mediated by homotropic inter-molecular receptor-receptor associations; (b) heterotropic associations with non-receptor scaffolding proteins or the subjacent cytoskeletal meshwork, leading to diffusional “trapping”, and (c) protein-lipid interactions, particularly with the neutral lipid cholesterol. Preceded by a brief introduction on the currently used methods to study protein lateral mobility in membranes, this review assesses the contribution of some of these mechanisms to the supramolecular organization and dynamics of the paradigm neurotransmitter receptor of muscle and neuronal cells—the nicotinic acetylcholine receptor (nAChR). The translational mobility of nAChRs at these two cell surfaces differs in terms of diffusion coefficients and residence intervals at the synapse, which cover an ample range of time regimes. Neuronal α7 nAChRs exhibit diffusion coefficients similar to those of other neurotransmitter receptors and spend part of their lifetime confined to the perisynaptic region of glutamatergic (excitatory) and GABAergic (inhibitory) synapses; they may also be involved in the regulation of the dynamic equilibrium between excitation and inhibition in brain