КОМПЛЕКСНАЯ АГРОЭКОЛОГИЧЕСКАЯ ОЦЕНКА ПРИМЕНЕНИЯ НЕТРАДИЦИОННЫХ УДОБРЕНИЙ

The article shows the results on soil recycling of municipal waste. The authors declare that application of unique fertilizers dosed economically efficient does not influence much the qualities of grey forest soil plough-layer. Single application of wastewater sludge dosed as 12t/ha increases humus concentration, cation exchange capacity and environmental pH becomes weakly alkaline. The paper finds out that efficient application of wastewater sludge doesn’t make effect on element burden and doesn’t break ecological balance. The authors conduct research on soil recycling of municipal waste with lime, which causes alkalinization of the soil in order to reduce migration mobility of heavy metals, which restrict their application. The research has shown that all parameters of total heavy metals were lower than admissible concentration unit in the years of fertilizing and in the following years. Soil pollution was minimal according to all criteria. The authors estimated bioenergetics efficiency of single application of wastewater sludge dosed as 12 t/ha when growing potato. Single application of wastewater sludge dosed as 12 t/ha demonstrated that wastewater sludge is high energy fertilizer. Stacking energy in extra yield at single application of wastewater sludge increased energy coefficient for the 3d year. This contributes to soil recycling of wastewater sludge as a fertilizer in case of biogeo-chemical monitoring of heavy metals concentration in the soil for growing environmentally sound production.Приведены результаты исследования почвенной утилизации бытовых отходов. Установлено, что применение нетрадиционных удобрений в экологически обоснованных дозах не оказывает значительного пролонгированного действия на свойства пахотного горизонта серых лесных почв. При одноразовом применении осадков сточных вод (ОСВ) в дозе 12 т/га повышается содержание гумуса, увеличивается ёмкость катионного обмена, pH среды смещается к слабощелочной. Показано, что рациональное использование ОСВ не оказывает значительного влияния на элементную нагрузку и не нарушает экологического равновесия. С целью снижения миграционной подвижности тяжелых металлов, ограничивающих их применение, проведены исследования почвенной утилизации ОСВ совместно с  известью, вызывающей подщелачивание почвы. Установлено, что все показатели валового содержания тяжелых металлов как в годы внесения удобрения, так и  в  последующие были ниже ПДК. Уровень загрязнения почвы по величине суммарного показателя концентрации по всем вариантам минимальный. Оценена биоэнергетическая эффективность и  определены расчётные коэффициенты энергетической эффективности при одноразовом применении ОСВ в дозе 12 т/га при выращивании картофеля. При однократном внесении ОСВ в дозе 12 т/га установлено, что они являются высокоэнергетическим перспективным удобрением. Энергия накопления в  прибавке урожая при одноразовом внесении ОСВ обеспечила наибольшее увеличение коэффициента энергетической эффективности на третий год последействия. Результаты исследований свидетельствуют о возможности почвенной утилизации ОСВ в качестве удобрений при условии организации биогеохимического мониторинга содержания тяжелых металлов в почве для выращивания экологически безопасной продукции

Proximity-dependent initiation of hybridization chain reaction

Sensitive detection of protein interactions and post-translational modifications of native proteins is a challenge for research and diagnostic purposes. A method for this, which could be used in point-of-care devices and high-throughput screening, should be reliable, cost effective and robust. To achieve this, here we design a method (proxHCR) that combines the need for proximal binding with hybridization chain reaction (HCR) for signal amplification. When two oligonucleotide hairpins conjugated to antibodies bind in close proximity, they can be activated to reveal an initiator sequence. This starts a chain reaction of hybridization events between a pair of fluorophore-labelled oligonucleotide hairpins, generating a fluorescent product. In conclusion, we show the applicability of the proxHCR method for the detection of protein interactions and posttranslational modifications in microscopy and flow cytometry. As no enzymes are needed, proxHCR may be an inexpensive and robust alternative to proximity ligation assays

Global assessment of aquatic Isoëtes species ecology

1. Isoetes are iconic but understudied wetland plants, despite having suffered severe losses globally mainly because of alterations in their habitats. We therefore provide the first global ecological assessment of aquatic Isoetes to identify their environmental requirements and to evaluate if taxonomically related species differ in their ecology. 2. The assessment resulted in an extensive new database on aquatic Isoetes, ecological niche analyses, and descriptive species accounts. We compiled a global database that includes all known environmental data collected from 1935 to 2023 regarding aquatic Isoetes. We then evaluated the environmental drivers of 16 species using 2,179 global records. Additionally, we used hypervolume analysis to quantify the ecological niches of the two species with the greatest number of records, finding significant differences and evidence that Isoetes echinospora occupies a wider ecological niche than Isoetes lacustris. 3. Fifty-nine species (30% of the c. 200 Isoetes species known today) were categorised as aquatic and were mainly reported in the Americas and northern Europe. About 38% of the aquatic species are threatened with extinction or are endemic to a small region, according to the International Union for Conservation of Nature's Red List in 2023. Many species were determined to be sensitive to certain water physical and chemical factors, generally preferring oligotrophic conditions such as low total phosphorus, moderate total nitrogen, moderate to low pH, and low conductivity. 4. This analysis includes ecological data in the assessment of rare/threatened aquatic plants globally. This new database and the ecological analyses completed defined the ecological requirements of several species and identified knowledge gaps, which can aid management actions and future research. 5. This paper highlights ecological significance and environmental sensitivities of aquatic Isoetes. The current level of knowledge is inadequate for a large proportion of known taxa. We affirm the extreme need to support global, collaborative initiatives on which to build future conservation strategies

ЭКОЛОГИЧЕСКАЯ РОЛЬ ЛЕСОВ В СИБИРИ

The article explores environmental situation in concern of the forests in Siberia and their role as they are very important in the economic aspect. The forests influence the natural processes in the country and bordering countries; they produce bioenergy and oxygen as well. The role of the forests is to influence ionic air regime, purify the air from hazardous gases and dust, reduce noise pollution level etc. The authors found out 1 m3 of the air in Siberian industrial cities contain from 100 to 500 thousands of dust and soot particles whereas the forests contain dust and soot particles 1000 times less. One hectare of forest can keep 32–68 tons of dust a year; developed wooden protective stands (40–45 m width) reduce noise pollution level from the transport on 17–23 dB. Volatile production of the forest appeared to be the important factor of their health-related features. The forests in extremely continental climate of Siberia influence such abiotic environmental factors as wind regime, solar radiation, air and soil temperature regime, precipitation, humidity etc. The publication shows that average yearly wind speed is up to 8 m/sec in bare Siberian tundra, 5 m/sec in sparsely wooded steppe (8 forest cover percent), 4 m/sec in the forest-steppe (18 forest cover percent) and 2.5–3 m/sec in taiga zone (40–50 forest cover percent). Abatement of wind speed influences better climate and V. V. The authors outline that beam solar radiation in the pinetum is 45 %, 30 % in the hardwood and 25 % in spruce forest. Increasing of forest cover percent from 30 to 70 affects low air temperature during vegetation period on 5…13 ºС in regards the average temperature of surrounding area. Soil temperature at a depth of 0.4 meter in December in the forest-steppe area of Western Siberia is –4,7 ºС, and in the southern taiga –1,2 ºС; soil temperature in June in the forest-steppe is +15,6 ºС, and +10 ºС in the southern taiga. Snow cover of leafed forests contains 15–30 % water more and softwood contains 5–10 % water more than in the open biota. Forests influence greatly hydrological regime of rivers. The total square of water-conservation forest in Siberia is 19.6 mln ha. Forests are greatly concerned with wind erosion control and dust whirls damaging the crops. Wind erosion areas in Western Siberia are spread in the steppe zone, mostly in Kulundinskaia steppe covering 17 mln ha. The article declares that crops and sowings margined with permeable shelterbelt produce crop yield 15–20 % higher than that in treeless areas.Приведено экологическое состояние и  роль лесных ресурсов в Сибири, которые имеют важное народно-хозяйственное значение. Они оказывают смягчающее воздействие на природные процессы на территории страны и смежных государств, являются аккумулятором и носителем биоэнергии и фабрикой кислорода. Роль лесов заключается также в способности положительно влиять на ионный режим воздуха, очищать его от вредных газов и пыли, снижать уровень шума и др. В 1 м3 воздуха сибирских индустриальных центров содержится от 100 до 500 тыс. частиц пыли и сажи, в лесу их почти в 1000 раз меньше. Гектар леса в состоянии задержать от 32 до 68 т пыли в год. Хорошо развитые древесные защитные насаждения шириной до 40–45 м снижают уровень шума от городского транспорта на 17–23 дБ. Важным фактором, обусловливающим лечебно-оздоровительные функции лесов, является их фитонцидность. В условиях резко-континентального климата Сибири лес активно влияет на такие абиотические факторы окружающей среды, как ветровой режим, солнечная радиация, температурный режим воздуха и почв, атмосферные осадки, влажность воздуха и др. Так, в безлесной сибирской тундре средняя годовая скорость ветра достигает 8 м/с, в малолесной степи (лесистость 8%)  – 5, в  лесостепной зоне (лесистость 18%) – 4, в лесной таежной зоне (лесистость 40–50%) – 2,5–3 м/с. Уменьшение силы ветра влияет на улучшение климата, и наоборот. Установлено, что по сравнению с открытой местностью прямая и рассеянная солнечная радиация в сосновом насаждении составляет 45%, в лиственном – 30, в еловом – 25. Увеличение лесистости с 30 до 70% обусловливает снижение температуры воздуха в течение вегетационного периода на 5–13ºС в сравнении со средней температурой окружающей местности. В лесостепной зоне Западной Сибири в декабре почва на глуби- не 0,4 м имеет температуру в среднем –4,7ºС, а в южной тайге –1,2ºС, в июне обратная картина; в лесостепи температура почвы +15,6ºС, в южной тайге +10 ºС. В лиственных лесах в снежном покрове накапливается воды на 15–30, а  в  хвойных – на 5–10% больше, чем на открытых биотопах. Леса оказывают мощное влияние на гидрологический режим рек. Общая площадь выделенных водоохранных лесов в Сибири составляет 19,6 млн га. Особенно велика роль лесов и лесных полос в борьбе с ветровой эрозией, с губительными для урожаев пыльными бурями. Районы действия ветровой эрозии в Западной Сибири распространены в степной зоне, преимущественно в Кулундинской степи, занимающей 17 млн га. Посевы зерновых и других сельскохозяйственных культур, окаймленные продуваемыми лесополосами, дают урожайность на 15–20% выше, чем посевы, размещенные на безлесных площадях

Bacterial Surface Appendages Strongly Impact Nanomechanical and Electrokinetic Properties of Escherichia coli Cells Subjected to Osmotic Stress

The physicochemical properties and dynamics of bacterial envelope, play a major role in bacterial activity. In this study, the morphological, nanomechanical and electrohydrodynamic properties of Escherichia coli K-12 mutant cells were thoroughly investigated as a function of bulk medium ionic strength using atomic force microscopy (AFM) and electrokinetics (electrophoresis). Bacteria were differing according to genetic alterations controlling the production of different surface appendages (short and rigid Ag43 adhesins, longer and more flexible type 1 fimbriae and F pilus). From the analysis of the spatially resolved force curves, it is shown that cells elasticity and turgor pressure are not only depending on bulk salt concentration but also on the presence/absence and nature of surface appendage. In 1 mM KNO3, cells without appendages or cells surrounded by Ag43 exhibit large Young moduli and turgor pressures (∼700–900 kPa and ∼100–300 kPa respectively). Under similar ionic strength condition, a dramatic ∼50% to ∼70% decrease of these nanomechanical parameters was evidenced for cells with appendages. Qualitatively, such dependence of nanomechanical behavior on surface organization remains when increasing medium salt content to 100 mM, even though, quantitatively, differences are marked to a much smaller extent. Additionally, for a given surface appendage, the magnitude of the nanomechanical parameters decreases significantly when increasing bulk salt concentration. This effect is ascribed to a bacterial exoosmotic water loss resulting in a combined contraction of bacterial cytoplasm together with an electrostatically-driven shrinkage of the surface appendages. The former process is demonstrated upon AFM analysis, while the latter, inaccessible upon AFM imaging, is inferred from electrophoretic data interpreted according to advanced soft particle electrokinetic theory. Altogether, AFM and electrokinetic results clearly demonstrate the intimate relationship between structure/flexibility and charge of bacterial envelope and propensity of bacterium and surface appendages to contract under hypertonic conditions