73 research outputs found
UV spectrophotometry method for the monitoring of galacto-oligosaccharides production
Monitoring the industrial production of galacto-oligosaccharides (GOS) requires a fast and accurate
methodology able to quantify, in real time, the substrate level and the product yield. In this work, a simple,
fast and inexpensive UV spectrophotometric method, together with partial least squares regression
(PLS) and artificial neural networks (ANN), was applied to simultaneously estimate the products (GOS)
and the substrate (lactose) concentrations in fermentation samples. The selected multiple models were
trained and their prediction abilities evaluated by cross-validation and external validation being the
results obtained compared with HPLC measurements. ANN models, generated from absorbance spectra
data of the fermentation samples, gave, in general, the best performance being able to accurately and precisely
predict lactose and total GOS levels, with standard error of prediction lower than 13 g kg 1 and
coefficient of determination for the external validation set of 0.93â0.94, showing residual predictive deviations
higher than five, whereas lower precision was obtained with the multiple model generated with
PLS. The results obtained show that UV spectrophotometry allowed an accurate and non-destructive
determination of sugars in fermentation samples and could be used as a fast alternative method for monitoring
GOS production.Fundação para a CiĂȘncia e a Tecnologia (FCT) - Bolsa de doutouramento SFRH/BDE/15510/2004AgĂȘncia da Inovação â Programa IDEIA (Potugal
α-D-glucan-based dendritic nanoparticles prepared by <i>in vitro</i> enzymatic chain extension of glycogen
International audienc
Structural characterization of the maltose acceptor-products synthesized by Leuconostoc mesentoeroides NRRL B-1299 dextransucrase
International audienc
Characterization of dextransucrases from Leuconostoc mesenteroides NRRL B-1299
International audienc
Characterization of the different dextransucrase activities excreted in glucose, fructose or sucrose medium by Leuconostoc mesentoeroides NRRL B-1299
International audienc
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