Expression profiles of acute lymphoblastic and myeloblastic leukemias with ALL-1 rearrangements

The ALL-1 gene is directly involved in 5-10% of ALLs and AMLs by fusion to other genes or through internal rearrangements. DNA microarrays were utilized to determine expression profiles of ALLs and AMLs with ALL-1 rearrangements. These profiles distinguish those tumors from other ALLs and AMLs. The expression patterns of ALL-1-associated tumors, in particular ALLs, involve oncogenes, tumor suppressors, anti apoptotic genes, drug resistance genes etc., and correlate with the aggressive nature of the tumors. The genes whose expression differentiates between ALLs with and without ALL-1 rearrangement were further divided into several groups enabling separation of ALL-1- associated ALLs into two subclasses. Further, AMLs with partial duplication of ALL-1 vary in their expression pattern from AMLs in which ALL-1 had undergone fusion to other genes. The extensive analysis described here draws attention to genes which might have a direct role in pathogenesis

Magnetic resonance imaging of anterior cruciate ligament rupture

BACKGROUND: Magnetic resonance (MR) imaging is a useful diagnostic tool for the assessment of knee joint injury. Anterior cruciate ligament repair is a commonly performed orthopaedic procedure. This paper examines the concordance between MR imaging and arthroscopic findings. METHODS: Between February, 1996 and February, 1998, 48 patients who underwent magnetic resonance (MR) imaging of the knee were reported to have complete tears of the anterior cruciate ligament (ACL). Of the 48 patients, 36 were male, and 12 female. The average age was 27 years (range: 15 to 45). Operative reconstruction using a patellar bone-tendon-bone autograft was arranged for each patient, and an arthroscopic examination was performed to confirm the diagnosis immediately prior to reconstructive surgery. RESULTS: In 16 of the 48 patients, reconstructive surgery was cancelled when incomplete lesions were noted during arthroscopy, making reconstructive surgery unnecessary. The remaining 32 patients were found to have complete tears of the ACL, and therefore underwent reconstructive surgery. Using arthroscopy as an independent, reliable reference standard for ACL tear diagnosis, the reliability of MR imaging was evaluated. The true positive rate for complete ACL tear diagnosis with MR imaging was 67%, making the possibility of a false-positive report of "complete ACL tear" inevitable with MR imaging. CONCLUSIONS: Since conservative treatment is sufficient for incomplete ACL tears, the decision to undertake ACL reconstruction should not be based on MR findings alone

Protocol requirements and diagnostic value of PET/MR imaging for liver metastasis detection

PURPOSE: To compare the accuracy of PET/MR imaging with that of FDG PET/CT and to determine the MR sequences necessary for the detection of liver metastasis using a trimodality PET/CT/MR set-up. METHODS: Included in this single-centre IRB-approved study were 55 patients (22 women, age 61 ± 11 years) with suspected liver metastases from gastrointestinal cancer. Imaging using a trimodality PET/CT/MR set-up (time-of-flight PET/CT and 3-T whole-body MR imager) comprised PET, low-dose CT, contrast-enhanced (CE) CT of the abdomen, and MR with T1-W/T2-W, diffusion-weighted (DWI), and dynamic CE imaging. Two readers evaluated the following image sets for liver metastasis: PET/CT (set A), PET/CECT (B), PET/MR including T1-W/T2-W (C), T1-W/T2-W with either DWI (D) or CE imaging (E), and a combination (F). The accuracy of each image set was determined by receiver-operating characteristic analysis using image set B as the standard of reference. RESULTS: Of 120 liver lesions in 21/55 patients (38 %), 79 (66 %) were considered malignant, and 63/79 (80 %) showed abnormal FDG uptake. Accuracies were 0.937 (95 % CI 89.5 - 97.9 %) for image set A, 1.00 (95 % CI 99.9 - 100.0 %) for set C, 0.998 (95 % CI 99.4 - 100.0 %) for set D, 0.997 (95 % CI 99.3 - 100.0 %) for set E, and 0.995 (95 % CI 99.0 - 100.0 %) for set F. Differences were significant for image sets D - F (P < 0.05) when including lesions without abnormal FDG uptake. As shown by follow-up imaging after 50 - 177 days, the use of image sets D and both sets E and F led to the detection of metastases in one and three patients, respectively, and further metastases in the contralateral lobe in two patients negative on PET/CECT (P = 0.06). CONCLUSION: PET/MR imaging with T1-W/T2-W sequences results in similar diagnostic accuracy for the detection of liver metastases to PET/CECT. To significantly improve the characterization of liver lesions, we recommend the use of dynamic CE imaging sequences. PET/MR imaging has a diagnostic impact on clinical decision making

Third Sonata Colors of July , Movement 3: Piano Solo Performed by Phyllis Rappeport

1 audio recording (6 minutes 9 seconds) Op. 11 (1999) : Third Sonata Colors of July”Movement 3 (of 3), composed by Emmanuel Dubois. Piano solo performed by WMU faculty member and pianist Phyllis Rappeport (1929-2020). Rappeport retired from Western Michigan University as Professor Emerita of Music in 1996.https://scholarworks.wmich.edu/emmanuel-dubois-compositions/1051/thumbnail.jp

Third Sonata Colors of July , Movement 2: Piano Solo Performed by Phyllis Rappeport

1 audio recording (4 minutes 52 seconds) Op. 11 (1999) : Third Sonata Colors of July”Movement 2 (of 3), composed by Emmanuel Dubois. Piano solo performed by WMU faculty member and pianist Phyllis Rappeport (1929-2020). Rappeport retired from Western Michigan University as Professor Emerita of Music in 1996.https://scholarworks.wmich.edu/emmanuel-dubois-compositions/1050/thumbnail.jp

First Sonata Colors of July”, Movement 2: Piano Solo Performed by Phyllis Rappeport

1 audio recording (5 minutes 37 seconds) Op 2a (1996, Rev. 2005) : First Sonata Colors of July”, Movement 2 (of 3), composed by Emmanuel Dubois. Piano solo performed by WMU faculty member and pianist Phyllis Rappeport (1929-2020). Rappeport retired from Western Michigan University as Professor Emerita of Music in 1996.https://scholarworks.wmich.edu/emmanuel-dubois-compositions/1043/thumbnail.jp

First Sonata Colors of July”, Movement 3: Piano Solo Performed by Phyllis Rappeport

1 audio recording (4 minutes 13 seconds) Op 2a (1996, Rev. 2005) : First Sonata Colors of July”, Movement 3 (of 3), composed by Emmanuel Dubois. Piano solo performed by WMU faculty member and pianist Phyllis Rappeport (1929-2020). Rappeport retired from Western Michigan University as Professor Emerita of Music in 1996.https://scholarworks.wmich.edu/emmanuel-dubois-compositions/1044/thumbnail.jp