Long-term follow-up after bariatric surgery in a national cohort

International audienc

Model of translocon implication in pancreatic beta cells.

<p>Chronic palmitate causes ER calcium depletion due to a greater ER calcium leak through the translocon, triggering ER stress and decreased glucose-stimulated insulin release, indicating an altered metabolism-secretion coupling. Addition of anisomycin reduced ER stress and ER calcium leak from translocon, leading to restored insulin secretion.</p

Anisomycin restores GSIS in palmitate condition in human islets.

<p>GSIS were measured after 48h BSA (white bar) + anisomycin (200 nM; grey bars) or palmitate (black bar) treatment + anisomycin (200 nM; hatched bars) in 6 human islet different preparations. *p<0.05.</p

Translocon inhibition partially prevents palmitate-induced reticular calcium depletion.

<p>Quantification of fluorescence ratio (F340/F380) of resting calcium (<b>a</b>) and of reticular calcium release induced by 1 μM thapsigargin (<b>b</b>) in 4 different human islets preparations (11 cells per preparation) under 48h BSA (white bar) + anisomycin (grey bars and palmitate (black bar) conditions + anisomycin (hatched bars). *p<0.05, **p<0.01.</p

Puromycin and anisomycin acute effects on calcium homeostasis in human islets.

<p>Puromycin and anisomcyin effects were evaluated in 4 different human islets preparation (44 different cells) (<b>a</b>-<b>e</b>). Typical cytosolic calcium traces in response to 200 μM thapsigargin (<b>a</b>) or to 1 μM puromycin (<b>b</b>) under control conditions and after 30 min incubation with 200 μM anisomycin. Cumulative data of peak cytosolic calcium increases evoked by puromycin responses under control conditions and with anisomycin 200 μM (<b>c</b>). Cumulative data of peak cytosolic calcium increases evoked by thapsigargin responses (<b>d</b>) and (<b>e</b>) resting fluorescence (F340/F380) under control conditions ± puromycin or anisomycin pretreatment. *p<0.05, **p<0.01. Measures were assessed in a calcium-free medium. Preparations were done in duplicate.</p

Translocon inhibition partially modulates palmitate-induced ER stress.

<p>ER stress mRNA expression markers were measured after 48h BSA (white bar) + anisomycin (grey bars) or palmitate (black bar) + anisomycin (hatched bars) treatment in 5 different human islet preparations. ***p<0.001;**p<0.01;*p<0.05.</p

Characteristics of human islet donors.

<p>Characteristics of human islet donors.</p