168 research outputs found
Incongruence between transcriptional and vascular pathophysiological cell states
Research in R.B.’s laboratory was supported by the European Research
Council Starting Grant AngioGenesHD (638028) and Consolidator
Grant AngioUnrestUHD (101001814), the CNIC Intramural Grant
Program Severo Ochoa (11-2016-IGP-SEV-2015-0505), the Ministerio
de Ciencia e Innovación (MCIN) (SAF2013-44329-P, RYC-2013-
13209, and SAF2017-89299-P) and ‘La Caixa’ Banking Foundation
(HR19-00120). J.V.’s laboratory was supported by MCIN (PGC2018-
097019-B-I00 and PID2021-122348NB-I00) and La Caixa (HR17-00247
and HR22-00253). K.G.’s laboratory was supported by Knut and
Alice Wallenberg Foundation (2020.0057) and Vetenskapsrådet
(2021-04896). The CNIC is supported by Instituto de Salud Carlos
III, MCIN, and the Pro CNIC Foundation, and is a Severo Ochoa
Center of Excellence (grant CEX2020-001041-S funded by MCIN/
AEI/10.13039/501100011033). Microscopy experiments were
performed at the Microscopy and Dynamic Imaging Unit, CNIC,
ICTS-ReDib, co-funded by MCIN/AEI/10.13039/501100011033 and
FEDER ‘Una manera de hacer Europa’ (ICTS-2018-04-CNIC-16). M.F.-C.
was supported by PhD fellowships from La Caixa (CX_E-2015-01)
and Boehringer Ingelheim travel grants. S.M. was supported by the
Austrian Science Fund (J4358). A.R. was supported by the Youth
Employment Initiative (PEJD-2019-PRE/BMD-16990). L.G.-O. was
supported by the Spanish Ministry of Economy and Competitiveness
(PRE2018-085283). We thank S. Bartlett (CNIC) for English editing,
as well as the members of the Transgenesis, Microscopy, Genomics,
Citometry and Bioinformatic units at CNIC. We also thank F. Radtke
(Swiss Institute for Experimental Cancer Research), R. H. Adams (Max
Planck Institute for Molecular Biomedicine), F. Alt (Boston Children’s
Hospital, Harvard Medical School), T. Honjo (Kyoto University Institute
for Advanced Studies), I. Flores (CNIC), J. Lewis (Cancer Research
UK London Research Institute), S. Habu (Tokai University School of
Medicine), T. Gridley (Maine Health Institute for Research) and C.
Brakebusch (Biotech Research and Innovation Centre) for sharing the
Dll4floxed, Notch1floxed, Notch2floxed, Cdh5(PAC)-creERT2, Myc floxed,
Rbpj floxed, p21−/−, Jag1floxed, Dll1floxed, Jag2floxed and Rac1floxed mice.S
Novel Avian Influenza H7N3 Strain Outbreak, British Columbia
Genome sequences of chicken (low pathogenic avian influenza [LPAI] and highly pathogenic avian influenza [HPAI]) and human isolates from a 2004 outbreak of H7N3 avian influenza in Canada showed a novel insertion in the HA0 cleavage site of the human and HPAI isolate. This insertion likely occurred by recombination between the hemagglutination and matrix genes in the LPAI virus
Thermolysin activation mutants with changes in the fusogenic region of an influenza virus hemagglutinin.
Influenza virus A/seal/Mass/1/80 (H7N7) mutants were obtained; the hemagglutinins (HAs) of the mutants were not activated by trypsin, as in the wild-type virus, but by thermolysin. The mutants grew efficiently under multiple replication cycle conditions and formed plaques in chicken embryo cells only when thermolysin was added to the culture medium. They exhibited hemolytic activity and induced protective immunity in chickens after an asymptomatic course of infection. Nucleotide sequencing of the HA gene and direct amino acid sequencing showed that insertion of a single leucine into the fusion peptide of the HA2 chain close to the cleavage site and a shift of the cleavage site toward the C terminus by one amino acid were responsible for the changes in the biological properties of the thermolysin activation mutants. Revertants could be obtained when trypsin or trypsin-like endoproteases were present in the virus-producing system
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